Asparagine--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

asparaginyl-tRNA synthetase, putative

Species:

Leishmania major

UniProt:

Q4Q2W6_LEIMA

TriTrypDb:

LmjF.34.2340 , LMJLV39_340028100 , LMJSD75_340029000

Length:

890

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

Q4Q2W6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q2W6

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006399	tRNA metabolic process	7	12
GO:0006418	tRNA aminoacylation for protein translation	6	12
GO:0006421	asparaginyl-tRNA aminoacylation	7	10
GO:0006520	amino acid metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0019752	carboxylic acid metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043038	amino acid activation	4	12
GO:0043039	tRNA aminoacylation	5	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043436	oxoacid metabolic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044281	small molecule metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901564	organonitrogen compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003676	nucleic acid binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004812	aminoacyl-tRNA ligase activity	4	12
GO:0004816	asparagine-tRNA ligase activity	5	10
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	826	830	PF00656	0.469
CLV_C14_Caspase3-7	834	838	PF00656	0.417
CLV_C14_Caspase3-7	874	878	PF00656	0.450
CLV_MEL_PAP_1	692	698	PF00089	0.262
CLV_NRD_NRD_1	118	120	PF00675	0.528
CLV_NRD_NRD_1	175	177	PF00675	0.703
CLV_NRD_NRD_1	380	382	PF00675	0.329
CLV_NRD_NRD_1	730	732	PF00675	0.235
CLV_NRD_NRD_1	848	850	PF00675	0.231
CLV_PCSK_FUR_1	173	177	PF00082	0.610
CLV_PCSK_KEX2_1	118	120	PF00082	0.563
CLV_PCSK_KEX2_1	175	177	PF00082	0.703
CLV_PCSK_KEX2_1	380	382	PF00082	0.249
CLV_PCSK_KEX2_1	656	658	PF00082	0.233
CLV_PCSK_KEX2_1	730	732	PF00082	0.216
CLV_PCSK_KEX2_1	832	834	PF00082	0.292
CLV_PCSK_KEX2_1	848	850	PF00082	0.330
CLV_PCSK_PC1ET2_1	656	658	PF00082	0.292
CLV_PCSK_PC1ET2_1	832	834	PF00082	0.329
CLV_PCSK_PC7_1	652	658	PF00082	0.239
CLV_PCSK_SKI1_1	246	250	PF00082	0.249
CLV_PCSK_SKI1_1	279	283	PF00082	0.235
CLV_PCSK_SKI1_1	380	384	PF00082	0.304
CLV_PCSK_SKI1_1	394	398	PF00082	0.370
CLV_PCSK_SKI1_1	92	96	PF00082	0.610
CLV_Separin_Metazoa	578	582	PF03568	0.467
DEG_APCC_DBOX_1	575	583	PF00400	0.439
DEG_SCF_FBW7_1	190	197	PF00400	0.552
DOC_CKS1_1	191	196	PF01111	0.554
DOC_CYCLIN_yCln2_LP_2	165	171	PF00134	0.588
DOC_CYCLIN_yCln2_LP_2	522	528	PF00134	0.435
DOC_MAPK_DCC_7	98	107	PF00069	0.459
DOC_MAPK_gen_1	254	264	PF00069	0.434
DOC_MAPK_gen_1	367	376	PF00069	0.529
DOC_MAPK_gen_1	470	479	PF00069	0.432
DOC_MAPK_gen_1	574	582	PF00069	0.439
DOC_MAPK_gen_1	730	740	PF00069	0.455
DOC_MAPK_MEF2A_6	98	107	PF00069	0.459
DOC_MAPK_RevD_3	163	176	PF00069	0.578
DOC_PP1_RVXF_1	862	869	PF00149	0.425
DOC_PP2B_LxvP_1	165	168	PF13499	0.655
DOC_PP2B_LxvP_1	522	525	PF13499	0.435
DOC_USP7_MATH_1	139	143	PF00917	0.673
DOC_USP7_MATH_1	210	214	PF00917	0.736
DOC_USP7_MATH_1	322	326	PF00917	0.550
DOC_USP7_MATH_1	331	335	PF00917	0.469
DOC_USP7_MATH_1	549	553	PF00917	0.474
DOC_USP7_MATH_1	61	65	PF00917	0.457
DOC_USP7_MATH_1	650	654	PF00917	0.456
DOC_WW_Pin1_4	153	158	PF00397	0.562
DOC_WW_Pin1_4	183	188	PF00397	0.622
DOC_WW_Pin1_4	190	195	PF00397	0.625
DOC_WW_Pin1_4	271	276	PF00397	0.429
DOC_WW_Pin1_4	445	450	PF00397	0.487
DOC_WW_Pin1_4	593	598	PF00397	0.435
DOC_WW_Pin1_4	721	726	PF00397	0.518
DOC_WW_Pin1_4	82	87	PF00397	0.739
LIG_14-3-3_CanoR_1	148	157	PF00244	0.643
LIG_14-3-3_CanoR_1	16	25	PF00244	0.601
LIG_14-3-3_CanoR_1	173	179	PF00244	0.604
LIG_14-3-3_CanoR_1	380	387	PF00244	0.525
LIG_14-3-3_CanoR_1	476	480	PF00244	0.435
LIG_14-3-3_CanoR_1	657	665	PF00244	0.427
LIG_14-3-3_CanoR_1	92	101	PF00244	0.717
LIG_Actin_WH2_2	372	388	PF00022	0.492
LIG_APCC_ABBA_1	526	531	PF00400	0.405
LIG_APCC_ABBA_1	580	585	PF00400	0.439
LIG_BRCT_BRCA1_1	608	612	PF00533	0.425
LIG_CtBP_PxDLS_1	49	53	PF00389	0.468
LIG_eIF4E_1	822	828	PF01652	0.529
LIG_FHA_1	195	201	PF00498	0.545
LIG_FHA_1	229	235	PF00498	0.425
LIG_FHA_1	297	303	PF00498	0.465
LIG_FHA_1	308	314	PF00498	0.397
LIG_FHA_1	356	362	PF00498	0.396
LIG_FHA_1	484	490	PF00498	0.516
LIG_FHA_1	54	60	PF00498	0.604
LIG_FHA_1	618	624	PF00498	0.425
LIG_FHA_1	633	639	PF00498	0.466
LIG_FHA_1	799	805	PF00498	0.455
LIG_FHA_2	33	39	PF00498	0.555
LIG_FHA_2	364	370	PF00498	0.556
LIG_FHA_2	601	607	PF00498	0.432
LIG_FHA_2	608	614	PF00498	0.416
LIG_FHA_2	661	667	PF00498	0.435
LIG_FHA_2	689	695	PF00498	0.512
LIG_FHA_2	797	803	PF00498	0.449
LIG_LIR_Gen_1	478	484	PF02991	0.429
LIG_LIR_Gen_1	531	540	PF02991	0.486
LIG_LIR_Gen_1	609	617	PF02991	0.425
LIG_LIR_Gen_1	660	669	PF02991	0.469
LIG_LIR_Gen_1	673	681	PF02991	0.535
LIG_LIR_Gen_1	682	693	PF02991	0.452
LIG_LIR_Gen_1	775	784	PF02991	0.529
LIG_LIR_Nem_3	436	441	PF02991	0.389
LIG_LIR_Nem_3	478	482	PF02991	0.429
LIG_LIR_Nem_3	531	536	PF02991	0.477
LIG_LIR_Nem_3	589	595	PF02991	0.538
LIG_LIR_Nem_3	609	615	PF02991	0.339
LIG_LIR_Nem_3	660	665	PF02991	0.493
LIG_LIR_Nem_3	673	677	PF02991	0.529
LIG_LIR_Nem_3	682	688	PF02991	0.452
LIG_LIR_Nem_3	726	732	PF02991	0.444
LIG_LIR_Nem_3	839	844	PF02991	0.425
LIG_MYND_1	86	90	PF01753	0.567
LIG_Pex14_1	588	592	PF04695	0.529
LIG_Pex14_2	583	587	PF04695	0.435
LIG_Pex14_2	778	782	PF04695	0.467
LIG_Rb_pABgroove_1	551	559	PF01858	0.404
LIG_RPA_C_Fungi	340	352	PF08784	0.279
LIG_SH2_CRK	533	537	PF00017	0.337
LIG_SH2_CRK	642	646	PF00017	0.207
LIG_SH2_NCK_1	533	537	PF00017	0.337
LIG_SH2_SRC	630	633	PF00017	0.386
LIG_SH2_SRC	734	737	PF00017	0.318
LIG_SH2_STAP1	734	738	PF00017	0.319
LIG_SH2_STAP1	824	828	PF00017	0.278
LIG_SH2_STAT3	622	625	PF00017	0.293
LIG_SH2_STAT3	844	847	PF00017	0.259
LIG_SH2_STAT5	463	466	PF00017	0.353
LIG_SH2_STAT5	622	625	PF00017	0.271
LIG_SH2_STAT5	630	633	PF00017	0.244
LIG_SH2_STAT5	642	645	PF00017	0.139
LIG_SH2_STAT5	844	847	PF00017	0.259
LIG_SH3_1	722	728	PF00018	0.266
LIG_SH3_2	725	730	PF14604	0.262
LIG_SH3_2	87	92	PF14604	0.613
LIG_SH3_3	184	190	PF00018	0.687
LIG_SH3_3	286	292	PF00018	0.358
LIG_SH3_3	443	449	PF00018	0.490
LIG_SH3_3	592	598	PF00018	0.259
LIG_SH3_3	712	718	PF00018	0.301
LIG_SH3_3	722	728	PF00018	0.318
LIG_SH3_3	792	798	PF00018	0.296
LIG_SH3_3	803	809	PF00018	0.250
LIG_SH3_3	84	90	PF00018	0.615
LIG_SH3_3	98	104	PF00018	0.463
LIG_SUMO_SIM_anti_2	801	808	PF11976	0.259
LIG_SUMO_SIM_par_1	103	108	PF11976	0.559
LIG_SUMO_SIM_par_1	358	366	PF11976	0.181
LIG_TRAF2_1	417	420	PF00917	0.745
LIG_TRAF2_2	705	710	PF00917	0.330
LIG_UBA3_1	869	876	PF00899	0.404
LIG_WRC_WIRS_1	298	303	PF05994	0.251
LIG_WRC_WIRS_1	671	676	PF05994	0.404
LIG_WRC_WIRS_1	786	791	PF05994	0.259
MOD_CDK_SPK_2	271	276	PF00069	0.259
MOD_CDK_SPxK_1	153	159	PF00069	0.441
MOD_CDK_SPxK_1	190	196	PF00069	0.660
MOD_CDK_SPxK_1	721	727	PF00069	0.404
MOD_CK1_1	130	136	PF00069	0.581
MOD_CK1_1	142	148	PF00069	0.684
MOD_CK1_1	152	158	PF00069	0.580
MOD_CK1_1	220	226	PF00069	0.641
MOD_CK1_1	475	481	PF00069	0.274
MOD_CK1_1	539	545	PF00069	0.403
MOD_CK1_1	660	666	PF00069	0.259
MOD_CK1_1	785	791	PF00069	0.279
MOD_CK1_1	80	86	PF00069	0.705
MOD_CK2_1	219	225	PF00069	0.548
MOD_CK2_1	250	256	PF00069	0.253
MOD_CK2_1	271	277	PF00069	0.243
MOD_CK2_1	32	38	PF00069	0.550
MOD_CK2_1	452	458	PF00069	0.405
MOD_CK2_1	600	606	PF00069	0.293
MOD_CK2_1	607	613	PF00069	0.293
MOD_CK2_1	660	666	PF00069	0.259
MOD_CK2_1	688	694	PF00069	0.371
MOD_CK2_1	796	802	PF00069	0.280
MOD_GlcNHglycan	132	135	PF01048	0.583
MOD_GlcNHglycan	210	213	PF01048	0.737
MOD_GlcNHglycan	219	222	PF01048	0.503
MOD_GlcNHglycan	333	336	PF01048	0.339
MOD_GSK3_1	135	142	PF00069	0.656
MOD_GSK3_1	149	156	PF00069	0.586
MOD_GSK3_1	190	197	PF00069	0.572
MOD_GSK3_1	215	222	PF00069	0.511
MOD_GSK3_1	327	334	PF00069	0.275
MOD_GSK3_1	545	552	PF00069	0.377
MOD_GSK3_1	564	571	PF00069	0.259
MOD_GSK3_1	596	603	PF00069	0.304
MOD_GSK3_1	617	624	PF00069	0.290
MOD_GSK3_1	62	69	PF00069	0.585
MOD_GSK3_1	630	637	PF00069	0.361
MOD_GSK3_1	684	691	PF00069	0.353
MOD_GSK3_1	77	84	PF00069	0.764
MOD_GSK3_1	796	803	PF00069	0.297
MOD_NEK2_1	112	117	PF00069	0.641
MOD_NEK2_1	135	140	PF00069	0.747
MOD_NEK2_1	208	213	PF00069	0.658
MOD_NEK2_1	307	312	PF00069	0.274
MOD_NEK2_1	568	573	PF00069	0.314
MOD_NEK2_1	600	605	PF00069	0.285
MOD_NEK2_1	623	628	PF00069	0.265
MOD_NEK2_1	66	71	PF00069	0.668
MOD_NEK2_1	684	689	PF00069	0.339
MOD_NEK2_1	81	86	PF00069	0.567
MOD_NEK2_1	815	820	PF00069	0.317
MOD_NEK2_1	836	841	PF00069	0.335
MOD_NEK2_1	97	102	PF00069	0.656
MOD_NEK2_2	607	612	PF00069	0.259
MOD_PIKK_1	307	313	PF00454	0.259
MOD_PIKK_1	600	606	PF00454	0.293
MOD_PIKK_1	621	627	PF00454	0.259
MOD_PIKK_1	703	709	PF00454	0.321
MOD_PK_1	18	24	PF00069	0.495
MOD_PKA_1	380	386	PF00069	0.181
MOD_PKA_2	17	23	PF00069	0.531
MOD_PKA_2	174	180	PF00069	0.557
MOD_PKA_2	380	386	PF00069	0.413
MOD_PKA_2	475	481	PF00069	0.274
MOD_PKA_2	501	507	PF00069	0.259
MOD_PKA_2	508	514	PF00069	0.259
MOD_PKA_2	564	570	PF00069	0.259
MOD_PKA_2	97	103	PF00069	0.505
MOD_PKB_1	16	24	PF00069	0.699
MOD_PKB_1	470	478	PF00069	0.259
MOD_Plk_1	549	555	PF00069	0.514
MOD_Plk_1	660	666	PF00069	0.259
MOD_Plk_1	836	842	PF00069	0.321
MOD_Plk_2-3	670	676	PF00069	0.366
MOD_Plk_2-3	796	802	PF00069	0.295
MOD_Plk_4	297	303	PF00069	0.369
MOD_Plk_4	32	38	PF00069	0.608
MOD_Plk_4	475	481	PF00069	0.395
MOD_Plk_4	542	548	PF00069	0.491
MOD_Plk_4	568	574	PF00069	0.353
MOD_Plk_4	596	602	PF00069	0.259
MOD_Plk_4	607	613	PF00069	0.259
MOD_Plk_4	62	68	PF00069	0.460
MOD_Plk_4	684	690	PF00069	0.361
MOD_Plk_4	785	791	PF00069	0.259
MOD_Plk_4	837	843	PF00069	0.305
MOD_ProDKin_1	153	159	PF00069	0.563
MOD_ProDKin_1	183	189	PF00069	0.621
MOD_ProDKin_1	190	196	PF00069	0.626
MOD_ProDKin_1	271	277	PF00069	0.265
MOD_ProDKin_1	445	451	PF00069	0.492
MOD_ProDKin_1	593	599	PF00069	0.274
MOD_ProDKin_1	721	727	PF00069	0.389
MOD_ProDKin_1	82	88	PF00069	0.741
MOD_SUMO_for_1	417	420	PF00179	0.665
MOD_SUMO_for_1	425	428	PF00179	0.666
MOD_SUMO_for_1	518	521	PF00179	0.304
MOD_SUMO_rev_2	271	281	PF00179	0.266
MOD_SUMO_rev_2	407	415	PF00179	0.563
TRG_DiLeu_BaLyEn_6	861	866	PF01217	0.318
TRG_ENDOCYTIC_2	533	536	PF00928	0.336
TRG_ENDOCYTIC_2	592	595	PF00928	0.404
TRG_ENDOCYTIC_2	642	645	PF00928	0.259
TRG_ENDOCYTIC_2	824	827	PF00928	0.278
TRG_ER_diArg_1	117	119	PF00400	0.634
TRG_ER_diArg_1	16	19	PF00400	0.568
TRG_ER_diArg_1	172	175	PF00400	0.707
TRG_ER_diArg_1	379	381	PF00400	0.404
TRG_ER_diArg_1	729	731	PF00400	0.264
TRG_ER_diArg_1	8	11	PF00400	0.640
TRG_ER_diArg_1	848	850	PF00400	0.407
TRG_NES_CRM1_1	400	411	PF08389	0.347
TRG_Pf-PMV_PEXEL_1	176	181	PF00026	0.475
TRG_Pf-PMV_PEXEL_1	657	661	PF00026	0.259

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMR3	Leptomonas seymouri	76%	100%
A0A0S4JDH2	Bodo saltans	59%	100%
A0A1X0P9Y4	Trypanosomatidae	64%	100%
A0A3R7P1H1	Trypanosoma rangeli	62%	100%
A0A3S7X7X7	Leishmania donovani	94%	100%
A4HAU6	Leishmania braziliensis	76%	99%
A4IA13	Leishmania infantum	94%	100%
C9ZMM2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	100%
E9B520	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
Q554D9	Dictyostelium discoideum	39%	100%
V5DGS5	Trypanosoma cruzi	63%	100%