RuvB-like helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

RuvB-like helicase

Gene product:

ruvB-like DNA helicase, putative

Species:

Leishmania major

UniProt:

Q4Q2T4_LEIMA

TriTrypDb:

LmjF.34.2610 , LMJLV39_340031500 , LMJSD75_340031600

Length:

483

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	yes	yes: 4
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000118	histone deacetylase complex	3	2
GO:0000123	histone acetyltransferase complex	4	12
GO:0000812	Swr1 complex	4	2
GO:0005634	nucleus	5	2
GO:0005737	cytoplasm	2	2
GO:0031011	Ino80 complex	5	12
GO:0031248	protein acetyltransferase complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0035267	NuA4 histone acetyltransferase complex	7	12
GO:0043189	H4/H2A histone acetyltransferase complex	6	12
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0070603	SWI/SNF superfamily-type complex	3	12
GO:0097255	R2TP complex	2	12
GO:0097346	INO80-type complex	4	12
GO:0110165	cellular anatomical entity	1	2
GO:0140513	nuclear protein-containing complex	2	12
GO:0140535	intracellular protein-containing complex	2	12
GO:1902493	acetyltransferase complex	4	12
GO:1902494	catalytic complex	2	12
GO:1902562	H4 histone acetyltransferase complex	5	12
GO:1904949	ATPase complex	3	12
GO:1990234	transferase complex	3	12

Expansion

Sequence features

Q4Q2T4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q2T4

Function

Biological processes
Term	Name	Level	Count
GO:0000491	small nucleolar ribonucleoprotein complex assembly	7	2
GO:0000492	box C/D snoRNP assembly	8	2
GO:0006325	chromatin organization	4	2
GO:0006338	chromatin remodeling	5	2
GO:0006355	regulation of DNA-templated transcription	6	2
GO:0006357	regulation of transcription by RNA polymerase II	7	2
GO:0006473	protein acetylation	6	2
GO:0006475	internal protein amino acid acetylation	7	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009889	regulation of biosynthetic process	4	2
GO:0009987	cellular process	1	2
GO:0010468	regulation of gene expression	5	2
GO:0010556	regulation of macromolecule biosynthetic process	5	2
GO:0016043	cellular component organization	3	2
GO:0016570	histone modification	5	2
GO:0016573	histone acetylation	6	2
GO:0018193	peptidyl-amino acid modification	5	2
GO:0018205	peptidyl-lysine modification	6	2
GO:0018393	internal peptidyl-lysine acetylation	8	2
GO:0018394	peptidyl-lysine acetylation	7	2
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	2
GO:0019222	regulation of metabolic process	3	2
GO:0019538	protein metabolic process	3	2
GO:0022607	cellular component assembly	4	2
GO:0022618	ribonucleoprotein complex assembly	6	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031326	regulation of cellular biosynthetic process	5	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043543	protein acylation	5	2
GO:0043933	protein-containing complex organization	4	2
GO:0044238	primary metabolic process	2	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051252	regulation of RNA metabolic process	5	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065003	protein-containing complex assembly	5	2
GO:0065007	biological regulation	1	2
GO:0071704	organic substance metabolic process	2	2
GO:0071826	ribonucleoprotein complex subunit organization	5	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:0080090	regulation of primary metabolic process	4	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1903506	regulation of nucleic acid-templated transcription	7	2
GO:2001141	regulation of RNA biosynthetic process	6	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003678	DNA helicase activity	3	12
GO:0003824	catalytic activity	1	12
GO:0004386	helicase activity	2	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0008094	ATP-dependent activity, acting on DNA	2	12
GO:0016462	pyrophosphatase activity	5	11
GO:0016787	hydrolase activity	2	12
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	11
GO:0016887	ATP hydrolysis activity	7	11
GO:0017076	purine nucleotide binding	4	12
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	11
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140097	catalytic activity, acting on DNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0140657	ATP-dependent activity	1	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	35	41	PF00089	0.388
CLV_NRD_NRD_1	122	124	PF00675	0.302
CLV_NRD_NRD_1	235	237	PF00675	0.302
CLV_NRD_NRD_1	416	418	PF00675	0.316
CLV_NRD_NRD_1	438	440	PF00675	0.432
CLV_NRD_NRD_1	51	53	PF00675	0.313
CLV_PCSK_KEX2_1	122	124	PF00082	0.302
CLV_PCSK_KEX2_1	235	237	PF00082	0.302
CLV_PCSK_KEX2_1	416	418	PF00082	0.302
CLV_PCSK_KEX2_1	438	440	PF00082	0.439
CLV_PCSK_KEX2_1	51	53	PF00082	0.313
CLV_PCSK_PC1ET2_1	235	237	PF00082	0.302
CLV_PCSK_PC1ET2_1	416	418	PF00082	0.302
CLV_PCSK_SKI1_1	12	16	PF00082	0.654
CLV_PCSK_SKI1_1	401	405	PF00082	0.302
CLV_PCSK_SKI1_1	449	453	PF00082	0.527
CLV_PCSK_SKI1_1	69	73	PF00082	0.312
DEG_APCC_DBOX_1	400	408	PF00400	0.502
DEG_SCF_FBW7_2	226	232	PF00400	0.502
DOC_CKS1_1	226	231	PF01111	0.502
DOC_CKS1_1	94	99	PF01111	0.565
DOC_CYCLIN_RxL_1	23	34	PF00134	0.502
DOC_CYCLIN_yCln2_LP_2	317	323	PF00134	0.502
DOC_MAPK_DCC_7	289	298	PF00069	0.502
DOC_MAPK_gen_1	122	129	PF00069	0.502
DOC_MAPK_gen_1	416	424	PF00069	0.493
DOC_MAPK_gen_1	428	435	PF00069	0.494
DOC_MAPK_gen_1	51	58	PF00069	0.503
DOC_MAPK_MEF2A_6	122	129	PF00069	0.502
DOC_MAPK_MEF2A_6	289	298	PF00069	0.502
DOC_MAPK_MEF2A_6	342	351	PF00069	0.502
DOC_MAPK_MEF2A_6	428	437	PF00069	0.508
DOC_MAPK_MEF2A_6	51	60	PF00069	0.502
DOC_USP7_MATH_1	37	41	PF00917	0.543
DOC_WW_Pin1_4	225	230	PF00397	0.502
DOC_WW_Pin1_4	316	321	PF00397	0.502
DOC_WW_Pin1_4	93	98	PF00397	0.493
LIG_14-3-3_CanoR_1	38	45	PF00244	0.502
LIG_14-3-3_CanoR_1	417	423	PF00244	0.502
LIG_14-3-3_CanoR_1	438	442	PF00244	0.445
LIG_Actin_WH2_2	387	403	PF00022	0.504
LIG_Actin_WH2_2	423	440	PF00022	0.502
LIG_APCC_ABBAyCdc20_2	449	455	PF00400	0.531
LIG_BIR_II_1	1	5	PF00653	0.628
LIG_BRCT_BRCA1_1	103	107	PF00533	0.502
LIG_BRCT_BRCA1_1	212	216	PF00533	0.563
LIG_Clathr_ClatBox_1	432	436	PF01394	0.607
LIG_FHA_1	112	118	PF00498	0.502
LIG_FHA_1	131	137	PF00498	0.502
LIG_FHA_1	157	163	PF00498	0.498
LIG_FHA_1	170	176	PF00498	0.478
LIG_FHA_1	198	204	PF00498	0.511
LIG_FHA_1	221	227	PF00498	0.502
LIG_FHA_1	240	246	PF00498	0.502
LIG_FHA_1	264	270	PF00498	0.502
LIG_FHA_1	419	425	PF00498	0.588
LIG_FHA_2	13	19	PF00498	0.618
LIG_FHA_2	242	248	PF00498	0.496
LIG_FHA_2	456	462	PF00498	0.499
LIG_IBAR_NPY_1	361	363	PF08397	0.607
LIG_LIR_Apic_2	339	344	PF02991	0.502
LIG_LIR_Gen_1	213	221	PF02991	0.545
LIG_LIR_Gen_1	308	317	PF02991	0.501
LIG_LIR_Gen_1	385	394	PF02991	0.588
LIG_LIR_Gen_1	95	106	PF02991	0.502
LIG_LIR_Nem_3	308	313	PF02991	0.501
LIG_LIR_Nem_3	385	391	PF02991	0.588
LIG_LIR_Nem_3	95	101	PF02991	0.494
LIG_Pex14_2	258	262	PF04695	0.502
LIG_SH2_CRK	341	345	PF00017	0.502
LIG_SH2_STAT3	402	405	PF00017	0.502
LIG_SH2_STAT5	402	405	PF00017	0.502
LIG_SH2_STAT5	431	434	PF00017	0.563
LIG_SH3_3	223	229	PF00018	0.502
LIG_SH3_3	317	323	PF00018	0.502
LIG_SH3_3	91	97	PF00018	0.586
LIG_SUMO_SIM_anti_2	247	253	PF11976	0.502
LIG_SUMO_SIM_anti_2	440	446	PF11976	0.474
LIG_SUMO_SIM_par_1	139	144	PF11976	0.513
LIG_SUMO_SIM_par_1	192	197	PF11976	0.502
LIG_SUMO_SIM_par_1	241	247	PF11976	0.496
LIG_SUMO_SIM_par_1	303	308	PF11976	0.502
LIG_SUMO_SIM_par_1	323	329	PF11976	0.563
LIG_SUMO_SIM_par_1	347	353	PF11976	0.502
LIG_SUMO_SIM_par_1	419	426	PF11976	0.516
LIG_TRAF2_1	364	367	PF00917	0.588
LIG_UBA3_1	106	113	PF00899	0.502
LIG_UBA3_1	179	187	PF00899	0.502
LIG_UBA3_1	390	397	PF00899	0.502
MOD_CK1_1	220	226	PF00069	0.494
MOD_CK1_1	420	426	PF00069	0.522
MOD_CK1_1	79	85	PF00069	0.588
MOD_CK2_1	109	115	PF00069	0.502
MOD_CK2_1	12	18	PF00069	0.629
MOD_CK2_1	164	170	PF00069	0.502
MOD_CK2_1	241	247	PF00069	0.502
MOD_CK2_1	361	367	PF00069	0.502
MOD_CK2_1	455	461	PF00069	0.664
MOD_GlcNHglycan	219	222	PF01048	0.354
MOD_GlcNHglycan	78	81	PF01048	0.388
MOD_GSK3_1	101	108	PF00069	0.499
MOD_GSK3_1	111	118	PF00069	0.488
MOD_GSK3_1	164	171	PF00069	0.516
MOD_GSK3_1	197	204	PF00069	0.512
MOD_GSK3_1	206	213	PF00069	0.475
MOD_GSK3_1	357	364	PF00069	0.502
MOD_GSK3_1	416	423	PF00069	0.588
MOD_LATS_1	128	134	PF00433	0.607
MOD_N-GLC_1	461	466	PF02516	0.750
MOD_NEK2_1	109	114	PF00069	0.502
MOD_NEK2_1	121	126	PF00069	0.502
MOD_NEK2_1	179	184	PF00069	0.494
MOD_NEK2_1	206	211	PF00069	0.502
MOD_NEK2_1	356	361	PF00069	0.607
MOD_NEK2_1	390	395	PF00069	0.513
MOD_NEK2_1	406	411	PF00069	0.457
MOD_PIKK_1	115	121	PF00454	0.502
MOD_PIKK_1	336	342	PF00454	0.527
MOD_PIKK_1	361	367	PF00454	0.592
MOD_PIKK_1	38	44	PF00454	0.502
MOD_PKA_1	416	422	PF00069	0.515
MOD_PKA_2	121	127	PF00069	0.502
MOD_PKA_2	146	152	PF00069	0.502
MOD_PKA_2	201	207	PF00069	0.573
MOD_PKA_2	336	342	PF00069	0.502
MOD_PKA_2	37	43	PF00069	0.513
MOD_PKA_2	416	422	PF00069	0.515
MOD_PKA_2	437	443	PF00069	0.501
MOD_Plk_1	12	18	PF00069	0.650
MOD_Plk_1	141	147	PF00069	0.511
MOD_Plk_1	169	175	PF00069	0.497
MOD_Plk_1	31	37	PF00069	0.588
MOD_Plk_1	455	461	PF00069	0.692
MOD_Plk_2-3	105	111	PF00069	0.502
MOD_Plk_2-3	192	198	PF00069	0.513
MOD_ProDKin_1	225	231	PF00069	0.502
MOD_ProDKin_1	316	322	PF00069	0.502
MOD_ProDKin_1	93	99	PF00069	0.493
MOD_SUMO_for_1	129	132	PF00179	0.502
MOD_SUMO_for_1	269	272	PF00179	0.502
MOD_SUMO_rev_2	265	271	PF00179	0.588
MOD_SUMO_rev_2	420	430	PF00179	0.502
TRG_DiLeu_BaEn_3	366	372	PF01217	0.551
TRG_DiLeu_BaEn_4	378	384	PF01217	0.588
TRG_ENDOCYTIC_2	431	434	PF00928	0.502
TRG_ER_diArg_1	121	123	PF00400	0.502
TRG_ER_diArg_1	437	439	PF00400	0.446
TRG_ER_diArg_1	465	468	PF00400	0.739
TRG_NES_CRM1_1	134	146	PF08389	0.607
TRG_NES_CRM1_1	425	436	PF08389	0.503
TRG_NLS_Bipartite_1	51	66	PF00514	0.607
TRG_Pf-PMV_PEXEL_1	27	32	PF00026	0.302

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I796	Leptomonas seymouri	94%	100%
A0A0N1IKW9	Leptomonas seymouri	43%	100%
A0A0S4JTM7	Bodo saltans	85%	100%
A0A0S4KMK6	Bodo saltans	44%	100%
A0A1X0P9Z8	Trypanosomatidae	45%	100%
A0A1X0PAP4	Trypanosomatidae	87%	100%
A0A3Q8IEM3	Leishmania donovani	43%	100%
A0A3Q8IFN2	Leishmania donovani	99%	100%
A0A422NEN7	Trypanosoma rangeli	44%	100%
A0A422NS36	Trypanosoma rangeli	86%	100%
A4HAX8	Leishmania braziliensis	97%	100%
A4HB68	Leishmania braziliensis	43%	100%
A4IA37	Leishmania infantum	99%	100%
A4IAB6	Leishmania infantum	43%	100%
C9ZLY0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	100%
C9ZM62	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	85%	100%
E9B552	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
E9B5D9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	43%	100%
O17607	Caenorhabditis elegans	43%	100%
O94692	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	64%	100%
P0CR26	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	44%	100%
P0CR27	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	44%	100%
P0CR28	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	65%	100%
P0CR29	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	65%	100%
P60122	Mus musculus	43%	100%
P60123	Rattus norvegicus	43%	100%
P83571	Danio rerio	69%	100%
Q03940	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	44%	100%
Q0IFL2	Aedes aegypti	43%	100%
Q12464	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	64%	100%
Q16TA2	Aedes aegypti	67%	100%
Q29AK9	Drosophila pseudoobscura pseudoobscura	44%	100%
Q29DI0	Drosophila pseudoobscura pseudoobscura	69%	100%
Q2TBU9	Bos taurus	71%	100%
Q4I948	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	61%	100%
Q4ICA8	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	44%	100%
Q4P112	Ustilago maydis (strain 521 / FGSC 9021)	43%	99%
Q4P6N7	Ustilago maydis (strain 521 / FGSC 9021)	64%	100%
Q4Q2J4	Leishmania major	43%	100%
Q4WKH9	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	62%	100%
Q4WPW8	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	44%	100%
Q54UW5	Dictyostelium discoideum	63%	100%
Q5A0W7	Candida albicans (strain SC5314 / ATCC MYA-2876)	45%	100%
Q5AGZ9	Candida albicans (strain SC5314 / ATCC MYA-2876)	62%	97%
Q5BBV9	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	44%	100%
Q5BGK3	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	61%	100%
Q6BI60	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	44%	100%
Q6BSB8	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	61%	100%
Q6C3X6	Yarrowia lipolytica (strain CLIB 122 / E 150)	62%	100%
Q6CB52	Yarrowia lipolytica (strain CLIB 122 / E 150)	45%	100%
Q6CQA9	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	45%	100%
Q6CT29	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	63%	100%
Q6FSF1	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	62%	100%
Q6FU78	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	44%	100%
Q750R1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	44%	100%
Q755G5	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	64%	100%
Q873C7	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	61%	100%
Q8AWW7	Danio rerio	43%	100%
Q8STP2	Encephalitozoon cuniculi (strain GB-M1)	41%	100%
Q8SU27	Encephalitozoon cuniculi (strain GB-M1)	45%	100%
Q8WZS3	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	43%	100%
Q9C0X6	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	43%	100%
Q9DE26	Xenopus laevis	44%	100%
Q9DE27	Xenopus laevis	69%	100%
Q9FMR9	Arabidopsis thaliana	43%	100%
Q9GZH2	Caenorhabditis elegans	55%	100%
Q9V3K3	Drosophila melanogaster	68%	100%
Q9VH07	Drosophila melanogaster	44%	100%
Q9WTM5	Mus musculus	71%	100%
Q9Y230	Homo sapiens	71%	100%
Q9Y265	Homo sapiens	43%	100%
V5B579	Trypanosoma cruzi	86%	100%
V5BQJ1	Trypanosoma cruzi	45%	100%