RNA helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

RNA helicase

Gene product:

ATP-dependent RNA and DNA helicase, mitochondrial, putative

Species:

Leishmania major

UniProt:

Q4Q2T3_LEIMA

TriTrypDb:

LmjF.34.2620 , LMJLV39_340031600 , LMJSD75_340031700

Length:

655

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0000177	cytoplasmic exosome (RNase complex)	5	2
GO:0000178	exosome (RNase complex)	4	2
GO:0032991	protein-containing complex	1	2
GO:0045025	mitochondrial degradosome	3	2
GO:0098798	mitochondrial protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:1905354	exoribonuclease complex	3	2
GO:0005739	mitochondrion	5	10
GO:0043226	organelle	2	10
GO:0043227	membrane-bounded organelle	3	10
GO:0043229	intracellular organelle	3	10
GO:0043231	intracellular membrane-bounded organelle	4	10
GO:0110165	cellular anatomical entity	1	11
GO:0005759	mitochondrial matrix	5	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1

Expansion

Sequence features

Q4Q2T3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q2T3

Function

Biological processes
Term	Name	Level	Count
GO:0000959	mitochondrial RNA metabolic process	6	2
GO:0000963	mitochondrial RNA processing	6	2
GO:0000965	mitochondrial RNA 3'-end processing	7	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006401	RNA catabolic process	5	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009987	cellular process	1	2
GO:0010467	gene expression	4	2
GO:0016070	RNA metabolic process	5	2
GO:0019439	aromatic compound catabolic process	4	2
GO:0031123	RNA 3'-end processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034655	nucleobase-containing compound catabolic process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0044270	cellular nitrogen compound catabolic process	4	2
GO:0046483	heterocycle metabolic process	3	2
GO:0046700	heterocycle catabolic process	4	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:0140053	mitochondrial gene expression	5	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901361	organic cyclic compound catabolic process	4	2
GO:1901575	organic substance catabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003724	RNA helicase activity	3	13
GO:0003824	catalytic activity	1	13
GO:0004386	helicase activity	2	13
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0008186	ATP-dependent activity, acting on RNA	2	13
GO:0016787	hydrolase activity	2	13
GO:0016817	hydrolase activity, acting on acid anhydrides	3	13
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	13
GO:0140640	catalytic activity, acting on a nucleic acid	2	13
GO:0140657	ATP-dependent activity	1	13
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:0016462	pyrophosphatase activity	5	1
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	1
GO:0016887	ATP hydrolysis activity	7	1
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	287	291	PF00656	0.399
CLV_MEL_PAP_1	623	629	PF00089	0.241
CLV_NRD_NRD_1	108	110	PF00675	0.356
CLV_NRD_NRD_1	241	243	PF00675	0.459
CLV_NRD_NRD_1	391	393	PF00675	0.431
CLV_NRD_NRD_1	473	475	PF00675	0.539
CLV_NRD_NRD_1	84	86	PF00675	0.468
CLV_PCSK_KEX2_1	107	109	PF00082	0.355
CLV_PCSK_KEX2_1	251	253	PF00082	0.420
CLV_PCSK_PC1ET2_1	251	253	PF00082	0.431
CLV_PCSK_PC7_1	104	110	PF00082	0.385
CLV_PCSK_SKI1_1	109	113	PF00082	0.352
CLV_PCSK_SKI1_1	210	214	PF00082	0.309
CLV_PCSK_SKI1_1	22	26	PF00082	0.535
CLV_PCSK_SKI1_1	283	287	PF00082	0.352
CLV_PCSK_SKI1_1	321	325	PF00082	0.484
CLV_PCSK_SKI1_1	366	370	PF00082	0.332
CLV_PCSK_SKI1_1	385	389	PF00082	0.302
CLV_PCSK_SKI1_1	512	516	PF00082	0.380
CLV_PCSK_SKI1_1	532	536	PF00082	0.232
CLV_Separin_Metazoa	522	526	PF03568	0.506
DEG_APCC_DBOX_1	241	249	PF00400	0.497
DEG_APCC_DBOX_1	288	296	PF00400	0.433
DOC_CYCLIN_RxL_1	509	519	PF00134	0.467
DOC_CYCLIN_RxL_1	522	531	PF00134	0.450
DOC_CYCLIN_RxL_1	633	643	PF00134	0.504
DOC_MAPK_gen_1	153	162	PF00069	0.443
DOC_MAPK_gen_1	251	258	PF00069	0.391
DOC_MAPK_gen_1	282	288	PF00069	0.452
DOC_MAPK_MEF2A_6	251	258	PF00069	0.395
DOC_MAPK_MEF2A_6	474	483	PF00069	0.529
DOC_PP1_RVXF_1	530	536	PF00149	0.420
DOC_PP2B_LxvP_1	213	216	PF13499	0.299
DOC_SPAK_OSR1_1	567	571	PF12202	0.435
DOC_USP7_MATH_1	116	120	PF00917	0.345
DOC_USP7_MATH_1	329	333	PF00917	0.593
DOC_USP7_MATH_1	342	346	PF00917	0.664
DOC_USP7_MATH_1	354	358	PF00917	0.745
DOC_USP7_MATH_1	411	415	PF00917	0.479
DOC_USP7_MATH_1	441	445	PF00917	0.436
DOC_USP7_UBL2_3	393	397	PF12436	0.411
DOC_USP7_UBL2_3	86	90	PF12436	0.523
DOC_WW_Pin1_4	179	184	PF00397	0.320
DOC_WW_Pin1_4	256	261	PF00397	0.390
DOC_WW_Pin1_4	345	350	PF00397	0.748
DOC_WW_Pin1_4	355	360	PF00397	0.597
DOC_WW_Pin1_4	475	480	PF00397	0.435
LIG_14-3-3_CanoR_1	385	394	PF00244	0.345
LIG_14-3-3_CanoR_1	427	431	PF00244	0.345
LIG_14-3-3_CanoR_1	474	479	PF00244	0.464
LIG_14-3-3_CanoR_1	525	530	PF00244	0.422
LIG_14-3-3_CanoR_1	626	632	PF00244	0.426
LIG_14-3-3_CanoR_1	77	84	PF00244	0.499
LIG_Actin_WH2_2	503	518	PF00022	0.442
LIG_APCC_ABBA_1	462	467	PF00400	0.496
LIG_BIR_II_1	1	5	PF00653	0.315
LIG_BRCT_BRCA1_1	413	417	PF00533	0.491
LIG_BRCT_BRCA1_1	501	505	PF00533	0.400
LIG_BRCT_BRCA1_1	56	60	PF00533	0.265
LIG_BRCT_BRCA1_1	623	627	PF00533	0.494
LIG_BRCT_BRCA1_2	623	629	PF00533	0.522
LIG_deltaCOP1_diTrp_1	203	208	PF00928	0.313
LIG_deltaCOP1_diTrp_1	53	60	PF00928	0.503
LIG_eIF4E_1	100	106	PF01652	0.463
LIG_FHA_1	207	213	PF00498	0.289
LIG_FHA_1	322	328	PF00498	0.439
LIG_FHA_1	363	369	PF00498	0.422
LIG_FHA_1	370	376	PF00498	0.293
LIG_FHA_1	456	462	PF00498	0.373
LIG_FHA_1	476	482	PF00498	0.362
LIG_FHA_1	528	534	PF00498	0.496
LIG_FHA_1	56	62	PF00498	0.475
LIG_FHA_1	643	649	PF00498	0.459
LIG_FHA_2	18	24	PF00498	0.463
LIG_FHA_2	180	186	PF00498	0.341
LIG_FHA_2	606	612	PF00498	0.367
LIG_FHA_2	628	634	PF00498	0.522
LIG_LIR_Apic_2	134	140	PF02991	0.310
LIG_LIR_Gen_1	275	285	PF02991	0.426
LIG_LIR_Gen_1	487	497	PF02991	0.463
LIG_LIR_Gen_1	618	627	PF02991	0.433
LIG_LIR_Gen_1	79	88	PF02991	0.534
LIG_LIR_Nem_3	275	280	PF02991	0.407
LIG_LIR_Nem_3	300	304	PF02991	0.478
LIG_LIR_Nem_3	487	491	PF02991	0.429
LIG_LIR_Nem_3	618	623	PF02991	0.433
LIG_LIR_Nem_3	79	84	PF02991	0.512
LIG_PCNA_yPIPBox_3	318	327	PF02747	0.487
LIG_PCNA_yPIPBox_3	380	393	PF02747	0.372
LIG_PCNA_yPIPBox_3	612	623	PF02747	0.484
LIG_Pex14_1	56	60	PF04695	0.480
LIG_Pex14_2	627	631	PF04695	0.424
LIG_PTB_Apo_2	231	238	PF02174	0.446
LIG_PTB_Phospho_1	231	237	PF10480	0.442
LIG_SH2_CRK	137	141	PF00017	0.307
LIG_SH2_GRB2like	326	329	PF00017	0.415
LIG_SH2_SRC	326	329	PF00017	0.415
LIG_SH2_STAT5	137	140	PF00017	0.297
LIG_SH2_STAT5	326	329	PF00017	0.504
LIG_SH2_STAT5	537	540	PF00017	0.443
LIG_SH2_STAT5	620	623	PF00017	0.424
LIG_SH3_3	181	187	PF00018	0.458
LIG_SH3_3	26	32	PF00018	0.352
LIG_SH3_3	397	403	PF00018	0.385
LIG_SUMO_SIM_anti_2	72	77	PF11976	0.390
LIG_SUMO_SIM_par_1	254	259	PF11976	0.422
LIG_SUMO_SIM_par_1	284	290	PF11976	0.447
LIG_SUMO_SIM_par_1	477	484	PF11976	0.526
LIG_UBA3_1	464	472	PF00899	0.502
LIG_WRC_WIRS_1	298	303	PF05994	0.238
MOD_CDK_SPxK_1	360	366	PF00069	0.533
MOD_CDK_SPxxK_3	256	263	PF00069	0.502
MOD_CK1_1	119	125	PF00069	0.344
MOD_CK1_1	261	267	PF00069	0.463
MOD_CK1_1	33	39	PF00069	0.513
MOD_CK1_1	345	351	PF00069	0.766
MOD_CK1_1	358	364	PF00069	0.626
MOD_CK1_1	470	476	PF00069	0.598
MOD_CK1_1	477	483	PF00069	0.629
MOD_CK1_1	484	490	PF00069	0.469
MOD_CK1_1	541	547	PF00069	0.316
MOD_CK1_1	65	71	PF00069	0.508
MOD_CK2_1	17	23	PF00069	0.391
MOD_CK2_1	243	249	PF00069	0.487
MOD_CK2_1	354	360	PF00069	0.712
MOD_CK2_1	484	490	PF00069	0.575
MOD_CK2_1	495	501	PF00069	0.513
MOD_CK2_1	605	611	PF00069	0.389
MOD_CK2_1	627	633	PF00069	0.382
MOD_CMANNOS	622	625	PF00535	0.264
MOD_GlcNHglycan	1	4	PF01048	0.589
MOD_GlcNHglycan	118	121	PF01048	0.335
MOD_GlcNHglycan	133	136	PF01048	0.332
MOD_GlcNHglycan	17	20	PF01048	0.314
MOD_GlcNHglycan	333	336	PF01048	0.590
MOD_GlcNHglycan	35	38	PF01048	0.373
MOD_GlcNHglycan	413	416	PF01048	0.499
MOD_GlcNHglycan	443	446	PF01048	0.526
MOD_GlcNHglycan	469	472	PF01048	0.516
MOD_GlcNHglycan	482	486	PF01048	0.567
MOD_GlcNHglycan	542	546	PF01048	0.382
MOD_GSK3_1	175	182	PF00069	0.325
MOD_GSK3_1	327	334	PF00069	0.527
MOD_GSK3_1	338	345	PF00069	0.577
MOD_GSK3_1	354	361	PF00069	0.534
MOD_GSK3_1	470	477	PF00069	0.569
MOD_GSK3_1	495	502	PF00069	0.403
MOD_GSK3_1	55	62	PF00069	0.442
MOD_GSK3_1	601	608	PF00069	0.547
MOD_GSK3_1	642	649	PF00069	0.326
MOD_GSK3_1	76	83	PF00069	0.473
MOD_N-GLC_1	243	248	PF02516	0.410
MOD_N-GLC_1	327	332	PF02516	0.537
MOD_N-GLC_1	338	343	PF02516	0.719
MOD_N-GLC_1	525	530	PF02516	0.337
MOD_N-GLC_2	10	12	PF02516	0.393
MOD_NEK2_1	386	391	PF00069	0.432
MOD_NEK2_1	417	422	PF00069	0.475
MOD_NEK2_1	481	486	PF00069	0.591
MOD_NEK2_1	495	500	PF00069	0.403
MOD_NEK2_1	61	66	PF00069	0.532
MOD_NEK2_1	627	632	PF00069	0.271
MOD_NEK2_1	640	645	PF00069	0.367
MOD_PIKK_1	499	505	PF00454	0.473
MOD_PIKK_1	66	72	PF00454	0.373
MOD_PIKK_1	76	82	PF00454	0.447
MOD_PKA_1	474	480	PF00069	0.462
MOD_PKA_2	354	360	PF00069	0.688
MOD_PKA_2	426	432	PF00069	0.349
MOD_PKA_2	76	82	PF00069	0.482
MOD_PKB_1	265	273	PF00069	0.419
MOD_Plk_1	243	249	PF00069	0.414
MOD_Plk_1	525	531	PF00069	0.378
MOD_Plk_2-3	605	611	PF00069	0.426
MOD_Plk_4	426	432	PF00069	0.463
MOD_Plk_4	484	490	PF00069	0.432
MOD_Plk_4	627	633	PF00069	0.306
MOD_ProDKin_1	179	185	PF00069	0.331
MOD_ProDKin_1	256	262	PF00069	0.386
MOD_ProDKin_1	345	351	PF00069	0.747
MOD_ProDKin_1	355	361	PF00069	0.592
MOD_ProDKin_1	475	481	PF00069	0.439
MOD_SUMO_rev_2	246	253	PF00179	0.468
MOD_SUMO_rev_2	633	643	PF00179	0.382
TRG_DiLeu_BaEn_1	249	254	PF01217	0.408
TRG_DiLeu_BaLyEn_6	227	232	PF01217	0.313
TRG_DiLeu_BaLyEn_6	36	41	PF01217	0.513
TRG_ENDOCYTIC_2	304	307	PF00928	0.362
TRG_ENDOCYTIC_2	537	540	PF00928	0.310
TRG_ENDOCYTIC_2	620	623	PF00928	0.275
TRG_ER_diArg_1	106	109	PF00400	0.379
TRG_ER_diArg_1	130	133	PF00400	0.420
TRG_ER_diArg_1	262	265	PF00400	0.571
TRG_NES_CRM1_1	519	531	PF08389	0.266
TRG_Pf-PMV_PEXEL_1	39	43	PF00026	0.450

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IL37	Leptomonas seymouri	68%	94%
A0A0S4JQD9	Bodo saltans	43%	100%
A0A0S4JTX4	Bodo saltans	43%	100%
A0A1X0PB30	Trypanosomatidae	55%	100%
A0A3R7MLZ5	Trypanosoma rangeli	52%	100%
A0A3S5H7X1	Leishmania donovani	94%	95%
A4HAX9	Leishmania braziliensis	84%	95%
A4IA38	Leishmania infantum	94%	95%
A4IG62	Danio rerio	32%	86%
C9ZM61	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9B553	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	95%
F4KFV7	Arabidopsis thaliana	32%	84%
O74727	Saccharomyces paradoxus	30%	89%
O94445	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
P32580	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	30%	89%
Q01IJ3	Oryza sativa subsp. indica	34%	89%
Q10D00	Oryza sativa subsp. japonica	32%	100%
Q17828	Caenorhabditis elegans	34%	91%
Q295E6	Drosophila pseudoobscura pseudoobscura	35%	86%
Q59TB2	Candida albicans (strain SC5314 / ATCC MYA-2876)	30%	91%
Q5EBA1	Rattus norvegicus	34%	84%
Q5ZJT0	Gallus gallus	32%	82%
Q61SU7	Caenorhabditis briggsae	33%	91%
Q7X745	Oryza sativa subsp. japonica	34%	89%
Q80YD1	Mus musculus	34%	84%
Q8IYB8	Homo sapiens	34%	83%
Q9SMX1	Arabidopsis thaliana	32%	100%
Q9VN03	Drosophila melanogaster	34%	86%
V5DLS8	Trypanosoma cruzi	52%	100%