DNA topoisomerase I

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

DNA topoisomerase I

Gene product:

DNA topoisomerase IB, large subunit

Species:

Leishmania major

UniProt:

Q4Q2K0_LEIMA

TriTrypDb:

LmjF.34.3440 , LMJLV39_340040100 , LMJSD75_340040400

Length:

634

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0005654	nucleoplasm	2	2
GO:0005694	chromosome	5	12
GO:0005730	nucleolus	5	2
GO:0016020	membrane	2	2
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043228	non-membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0043232	intracellular non-membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

Q4Q2K0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q2K0

PDB structure(s): 2b9s_A

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006260	DNA replication	5	2
GO:0006265	DNA topological change	5	12
GO:0006355	regulation of DNA-templated transcription	6	2
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006996	organelle organization	4	12
GO:0007059	chromosome segregation	2	2
GO:0008152	metabolic process	1	12
GO:0009889	regulation of biosynthetic process	4	2
GO:0009987	cellular process	1	12
GO:0010468	regulation of gene expression	5	2
GO:0010556	regulation of macromolecule biosynthetic process	5	2
GO:0016043	cellular component organization	3	12
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	2
GO:0019222	regulation of metabolic process	3	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031326	regulation of cellular biosynthetic process	5	2
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051252	regulation of RNA metabolic process	5	2
GO:0051276	chromosome organization	5	12
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065007	biological regulation	1	2
GO:0071103	DNA conformation change	6	12
GO:0071704	organic substance metabolic process	2	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0080090	regulation of primary metabolic process	4	2
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1903506	regulation of nucleic acid-templated transcription	7	2
GO:2001141	regulation of RNA biosynthetic process	6	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003677	DNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0003916	DNA topoisomerase activity	3	12
GO:0003917	DNA topoisomerase type I (single strand cut, ATP-independent) activity	4	12
GO:0005488	binding	1	12
GO:0016853	isomerase activity	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140097	catalytic activity, acting on DNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	155	159	PF00656	0.243
CLV_C14_Caspase3-7	399	403	PF00656	0.337
CLV_MEL_PAP_1	311	317	PF00089	0.222
CLV_NRD_NRD_1	102	104	PF00675	0.252
CLV_NRD_NRD_1	121	123	PF00675	0.281
CLV_NRD_NRD_1	172	174	PF00675	0.368
CLV_NRD_NRD_1	273	275	PF00675	0.259
CLV_NRD_NRD_1	376	378	PF00675	0.257
CLV_NRD_NRD_1	44	46	PF00675	0.368
CLV_NRD_NRD_1	475	477	PF00675	0.276
CLV_NRD_NRD_1	578	580	PF00675	0.630
CLV_PCSK_KEX2_1	102	104	PF00082	0.262
CLV_PCSK_KEX2_1	11	13	PF00082	0.625
CLV_PCSK_KEX2_1	115	117	PF00082	0.285
CLV_PCSK_KEX2_1	121	123	PF00082	0.255
CLV_PCSK_KEX2_1	172	174	PF00082	0.361
CLV_PCSK_KEX2_1	44	46	PF00082	0.222
CLV_PCSK_KEX2_1	531	533	PF00082	0.503
CLV_PCSK_KEX2_1	587	589	PF00082	0.649
CLV_PCSK_KEX2_1	613	615	PF00082	0.587
CLV_PCSK_PC1ET2_1	11	13	PF00082	0.625
CLV_PCSK_PC1ET2_1	115	117	PF00082	0.256
CLV_PCSK_PC1ET2_1	531	533	PF00082	0.503
CLV_PCSK_PC1ET2_1	587	589	PF00082	0.649
CLV_PCSK_PC1ET2_1	613	615	PF00082	0.582
CLV_PCSK_SKI1_1	103	107	PF00082	0.222
CLV_PCSK_SKI1_1	110	114	PF00082	0.222
CLV_PCSK_SKI1_1	151	155	PF00082	0.306
CLV_PCSK_SKI1_1	274	278	PF00082	0.222
CLV_PCSK_SKI1_1	310	314	PF00082	0.222
CLV_PCSK_SKI1_1	420	424	PF00082	0.252
CLV_PCSK_SKI1_1	459	463	PF00082	0.368
CLV_PCSK_SKI1_1	499	503	PF00082	0.465
CLV_PCSK_SKI1_1	548	552	PF00082	0.667
CLV_PCSK_SKI1_1	558	562	PF00082	0.552
CLV_PCSK_SKI1_1	564	568	PF00082	0.602
CLV_PCSK_SKI1_1	616	620	PF00082	0.600
CLV_PCSK_SKI1_1	72	76	PF00082	0.256
DEG_APCC_DBOX_1	475	483	PF00400	0.156
DEG_MDM2_SWIB_1	105	113	PF02201	0.222
DEG_Nend_UBRbox_1	1	4	PF02207	0.660
DEG_SCF_FBW7_2	76	81	PF00400	0.222
DEG_SPOP_SBC_1	256	260	PF00917	0.256
DOC_ANK_TNKS_1	179	186	PF00023	0.237
DOC_CYCLIN_RxL_1	115	129	PF00134	0.223
DOC_CYCLIN_RxL_1	471	483	PF00134	0.156
DOC_MAPK_gen_1	377	384	PF00069	0.229
DOC_MAPK_gen_1	459	468	PF00069	0.287
DOC_MAPK_gen_1	613	620	PF00069	0.482
DOC_PP2B_LxvP_1	55	58	PF13499	0.316
DOC_USP7_MATH_1	257	261	PF00917	0.336
DOC_USP7_MATH_1	290	294	PF00917	0.280
DOC_USP7_MATH_1	566	570	PF00917	0.618
DOC_USP7_MATH_1	578	582	PF00917	0.666
DOC_USP7_MATH_1	586	590	PF00917	0.615
DOC_USP7_MATH_1	598	602	PF00917	0.613
DOC_USP7_UBL2_3	147	151	PF12436	0.224
DOC_USP7_UBL2_3	275	279	PF12436	0.380
DOC_USP7_UBL2_3	455	459	PF12436	0.256
DOC_USP7_UBL2_3	563	567	PF12436	0.566
DOC_USP7_UBL2_3	571	575	PF12436	0.531
DOC_USP7_UBL2_3	576	580	PF12436	0.594
DOC_USP7_UBL2_3	583	587	PF12436	0.500
DOC_USP7_UBL2_3	7	11	PF12436	0.590
DOC_WW_Pin1_4	376	381	PF00397	0.282
DOC_WW_Pin1_4	74	79	PF00397	0.234
LIG_14-3-3_CanoR_1	284	288	PF00244	0.279
LIG_14-3-3_CanoR_1	373	380	PF00244	0.280
LIG_14-3-3_CanoR_1	476	480	PF00244	0.255
LIG_14-3-3_CanoR_1	579	586	PF00244	0.496
LIG_APCC_ABBA_1	65	70	PF00400	0.270
LIG_BRCT_BRCA1_1	457	461	PF00533	0.368
LIG_CaM_IQ_9	463	479	PF13499	0.287
LIG_EVH1_2	183	187	PF00568	0.237
LIG_FHA_1	207	213	PF00498	0.249
LIG_FHA_1	476	482	PF00498	0.264
LIG_FHA_1	607	613	PF00498	0.699
LIG_FHA_2	144	150	PF00498	0.222
LIG_FHA_2	468	474	PF00498	0.350
LIG_FHA_2	75	81	PF00498	0.222
LIG_HCF-1_HBM_1	92	95	PF13415	0.237
LIG_LIR_Gen_1	235	246	PF02991	0.243
LIG_LIR_Gen_1	366	374	PF02991	0.299
LIG_LIR_Gen_1	383	393	PF02991	0.146
LIG_LIR_Gen_1	43	54	PF02991	0.237
LIG_LIR_Gen_1	524	533	PF02991	0.392
LIG_LIR_Gen_1	85	95	PF02991	0.222
LIG_LIR_Nem_3	107	112	PF02991	0.224
LIG_LIR_Nem_3	127	131	PF02991	0.282
LIG_LIR_Nem_3	366	371	PF02991	0.269
LIG_LIR_Nem_3	383	388	PF02991	0.145
LIG_LIR_Nem_3	43	49	PF02991	0.236
LIG_LIR_Nem_3	524	528	PF02991	0.476
LIG_PDZ_Class_2	629	634	PF00595	0.584
LIG_Pex14_1	512	516	PF04695	0.379
LIG_Pex14_2	101	105	PF04695	0.222
LIG_SH2_CRK	368	372	PF00017	0.282
LIG_SH2_GRB2like	16	19	PF00017	0.436
LIG_SH2_GRB2like	357	360	PF00017	0.237
LIG_SH2_GRB2like	439	442	PF00017	0.222
LIG_SH2_PTP2	252	255	PF00017	0.222
LIG_SH2_SRC	60	63	PF00017	0.282
LIG_SH2_STAT3	135	138	PF00017	0.156
LIG_SH2_STAT5	135	138	PF00017	0.391
LIG_SH2_STAT5	166	169	PF00017	0.294
LIG_SH2_STAT5	252	255	PF00017	0.222
LIG_SH2_STAT5	306	309	PF00017	0.280
LIG_SH2_STAT5	439	442	PF00017	0.236
LIG_SH2_STAT5	474	477	PF00017	0.256
LIG_SH3_1	423	429	PF00018	0.216
LIG_SH3_3	178	184	PF00018	0.237
LIG_SH3_3	215	221	PF00018	0.271
LIG_SH3_3	423	429	PF00018	0.216
LIG_SH3_3	51	57	PF00018	0.226
LIG_SUMO_SIM_anti_2	360	366	PF11976	0.340
LIG_SUMO_SIM_anti_2	445	451	PF11976	0.256
LIG_SUMO_SIM_anti_2	478	483	PF11976	0.337
LIG_SUMO_SIM_par_1	477	483	PF11976	0.359
LIG_TRAF2_1	146	149	PF00917	0.222
LIG_TRAF2_1	493	496	PF00917	0.470
LIG_TRAF2_1	589	592	PF00917	0.554
LIG_TRAF2_1	77	80	PF00917	0.223
LIG_TRAF2_2	390	395	PF00917	0.368
LIG_UBA3_1	195	200	PF00899	0.249
LIG_UBA3_1	465	471	PF00899	0.350
LIG_UBA3_1	599	607	PF00899	0.626
LIG_UBA3_1	86	91	PF00899	0.368
LIG_WRC_WIRS_1	397	402	PF05994	0.227
LIG_WW_1	57	60	PF00397	0.237
MOD_CK1_1	472	478	PF00069	0.243
MOD_CK1_1	515	521	PF00069	0.523
MOD_CK1_1	544	550	PF00069	0.552
MOD_CK1_1	554	560	PF00069	0.487
MOD_CK2_1	105	111	PF00069	0.237
MOD_CK2_1	143	149	PF00069	0.222
MOD_CK2_1	283	289	PF00069	0.350
MOD_CK2_1	3	9	PF00069	0.598
MOD_CK2_1	467	473	PF00069	0.436
MOD_CK2_1	490	496	PF00069	0.535
MOD_CK2_1	586	592	PF00069	0.643
MOD_CK2_1	74	80	PF00069	0.222
MOD_Cter_Amidation	113	116	PF01082	0.156
MOD_Cter_Amidation	170	173	PF01082	0.222
MOD_GlcNHglycan	404	407	PF01048	0.241
MOD_GlcNHglycan	544	547	PF01048	0.610
MOD_GlcNHglycan	551	554	PF01048	0.511
MOD_GlcNHglycan	564	567	PF01048	0.507
MOD_GlcNHglycan	568	571	PF01048	0.616
MOD_GSK3_1	372	379	PF00069	0.382
MOD_GSK3_1	408	415	PF00069	0.222
MOD_GSK3_1	536	543	PF00069	0.498
MOD_GSK3_1	554	561	PF00069	0.526
MOD_GSK3_1	562	569	PF00069	0.555
MOD_LATS_1	453	459	PF00433	0.256
MOD_N-GLC_1	412	417	PF02516	0.222
MOD_NEK2_1	105	110	PF00069	0.237
MOD_NEK2_1	466	471	PF00069	0.384
MOD_NEK2_2	257	262	PF00069	0.237
MOD_NEK2_2	328	333	PF00069	0.222
MOD_NEK2_2	351	356	PF00069	0.237
MOD_PIKK_1	388	394	PF00454	0.368
MOD_PK_1	490	496	PF00069	0.535
MOD_PKA_1	141	147	PF00069	0.220
MOD_PKA_1	490	496	PF00069	0.464
MOD_PKA_1	541	547	PF00069	0.625
MOD_PKA_1	562	568	PF00069	0.626
MOD_PKA_1	579	585	PF00069	0.620
MOD_PKA_2	283	289	PF00069	0.237
MOD_PKA_2	372	378	PF00069	0.256
MOD_PKA_2	475	481	PF00069	0.315
MOD_PKA_2	578	584	PF00069	0.500
MOD_Plk_1	12	18	PF00069	0.451
MOD_Plk_1	206	212	PF00069	0.234
MOD_Plk_1	232	238	PF00069	0.277
MOD_Plk_1	412	418	PF00069	0.222
MOD_Plk_4	234	240	PF00069	0.231
MOD_Plk_4	283	289	PF00069	0.361
MOD_Plk_4	396	402	PF00069	0.242
MOD_Plk_4	82	88	PF00069	0.222
MOD_ProDKin_1	376	382	PF00069	0.282
MOD_ProDKin_1	74	80	PF00069	0.234
MOD_SUMO_for_1	1	4	PF00179	0.613
MOD_SUMO_for_1	10	13	PF00179	0.605
MOD_SUMO_for_1	268	271	PF00179	0.222
MOD_SUMO_for_1	40	43	PF00179	0.483
MOD_SUMO_rev_2	144	153	PF00179	0.294
MOD_SUMO_rev_2	424	432	PF00179	0.353
MOD_SUMO_rev_2	539	544	PF00179	0.537
MOD_SUMO_rev_2	70	77	PF00179	0.237
TRG_DiLeu_BaEn_1	335	340	PF01217	0.368
TRG_DiLeu_BaEn_1	366	371	PF01217	0.368
TRG_ENDOCYTIC_2	252	255	PF00928	0.224
TRG_ENDOCYTIC_2	368	371	PF00928	0.237
TRG_ENDOCYTIC_2	95	98	PF00928	0.368
TRG_ER_diArg_1	101	103	PF00400	0.268
TRG_ER_diArg_1	120	122	PF00400	0.279
TRG_ER_diArg_1	187	190	PF00400	0.222
TRG_ER_diArg_1	44	46	PF00400	0.368
TRG_ER_diArg_1	535	538	PF00400	0.529
TRG_NLS_Bipartite_1	476	494	PF00514	0.413
TRG_NLS_Bipartite_1	531	552	PF00514	0.605
TRG_NLS_MonoExtC_3	489	495	PF00514	0.510
TRG_Pf-PMV_PEXEL_1	274	278	PF00026	0.316

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0L0P4F8	Candida auris	50%	85%
A0A0N0P5Q3	Leptomonas seymouri	79%	98%
A0A0S4IPT9	Bodo saltans	55%	98%
A0A1X0PA36	Trypanosomatidae	65%	95%
A0A3Q8IHS4	Leishmania donovani	96%	100%
A0A3R7MDP0	Trypanosoma rangeli	65%	95%
A4HB62	Leishmania braziliensis	89%	100%
A9Q1D5	Pan troglodytes	44%	100%
C9ZLY6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	91%
E9B5D3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
O17966	Caenorhabditis elegans	49%	79%
P04786	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	44%	82%
P07799	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	54%	78%
P11387	Homo sapiens	51%	83%
P30181	Arabidopsis thaliana	52%	69%
P41512	Xenopus laevis	48%	76%
P93119	Daucus carota	49%	80%
Q00313	Candida albicans	49%	81%
Q04750	Mus musculus	51%	83%
Q07050	Cricetulus griseus	51%	83%
Q54RC3	Dictyostelium discoideum	48%	71%
Q6IM78	Rattus norvegicus	45%	100%
Q7YR26	Chlorocebus aethiops	51%	83%
Q8R4U6	Mus musculus	46%	100%
Q969P6	Homo sapiens	44%	100%
Q9FJ79	Arabidopsis thaliana	53%	69%
Q9GPZ9	Leishmania infantum	96%	100%
Q9WUL0	Rattus norvegicus	51%	83%
V5BQI4	Trypanosoma cruzi	63%	95%