RuvB-like helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

RuvB-like helicase

Gene product:

ruvb-like 1 DNA helicase, putative

Species:

Leishmania major

UniProt:

Q4Q2J4_LEIMA

TriTrypDb:

LmjF.34.3500 , LMJLV39_340040700 , LMJSD75_340041000

Length:

459

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	yes	yes: 4
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000118	histone deacetylase complex	3	2
GO:0000123	histone acetyltransferase complex	4	12
GO:0000812	Swr1 complex	4	2
GO:0005654	nucleoplasm	2	2
GO:0005737	cytoplasm	2	2
GO:0031011	Ino80 complex	5	12
GO:0031248	protein acetyltransferase complex	3	12
GO:0031974	membrane-enclosed lumen	2	2
GO:0031981	nuclear lumen	5	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0032991	protein-containing complex	1	12
GO:0035267	NuA4 histone acetyltransferase complex	7	12
GO:0043189	H4/H2A histone acetyltransferase complex	6	12
GO:0043233	organelle lumen	3	2
GO:0070013	intracellular organelle lumen	4	2
GO:0070603	SWI/SNF superfamily-type complex	3	12
GO:0097014	ciliary plasm	5	2
GO:0097255	R2TP complex	2	12
GO:0097346	INO80-type complex	4	12
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	2
GO:0140513	nuclear protein-containing complex	2	12
GO:0140535	intracellular protein-containing complex	2	12
GO:1902493	acetyltransferase complex	4	12
GO:1902494	catalytic complex	2	12
GO:1902562	H4 histone acetyltransferase complex	5	12
GO:1904949	ATPase complex	3	12
GO:1990234	transferase complex	3	12

Expansion

Sequence features

Q4Q2J4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q2J4

Function

Biological processes
Term	Name	Level	Count
GO:0000491	small nucleolar ribonucleoprotein complex assembly	7	2
GO:0000492	box C/D snoRNP assembly	8	2
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006281	DNA repair	5	12
GO:0006325	chromatin organization	4	2
GO:0006338	chromatin remodeling	5	2
GO:0006355	regulation of DNA-templated transcription	6	2
GO:0006357	regulation of transcription by RNA polymerase II	7	2
GO:0006473	protein acetylation	6	2
GO:0006475	internal protein amino acid acetylation	7	2
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0006974	DNA damage response	4	12
GO:0008152	metabolic process	1	12
GO:0009889	regulation of biosynthetic process	4	2
GO:0009987	cellular process	1	12
GO:0010468	regulation of gene expression	5	2
GO:0010556	regulation of macromolecule biosynthetic process	5	2
GO:0016043	cellular component organization	3	2
GO:0016570	histone modification	5	2
GO:0016573	histone acetylation	6	2
GO:0018193	peptidyl-amino acid modification	5	2
GO:0018205	peptidyl-lysine modification	6	2
GO:0018393	internal peptidyl-lysine acetylation	8	2
GO:0018394	peptidyl-lysine acetylation	7	2
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	2
GO:0019222	regulation of metabolic process	3	2
GO:0019538	protein metabolic process	3	2
GO:0022607	cellular component assembly	4	2
GO:0022618	ribonucleoprotein complex assembly	6	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031326	regulation of cellular biosynthetic process	5	2
GO:0033554	cellular response to stress	3	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	2
GO:0043543	protein acylation	5	2
GO:0043933	protein-containing complex organization	4	2
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0050896	response to stimulus	1	12
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051252	regulation of RNA metabolic process	5	2
GO:0051716	cellular response to stimulus	2	12
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065003	protein-containing complex assembly	5	2
GO:0065007	biological regulation	1	2
GO:0071704	organic substance metabolic process	2	12
GO:0071826	ribonucleoprotein complex subunit organization	5	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:0080090	regulation of primary metabolic process	4	2
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1903506	regulation of nucleic acid-templated transcription	7	2
GO:2001141	regulation of RNA biosynthetic process	6	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003678	DNA helicase activity	3	12
GO:0003824	catalytic activity	1	12
GO:0004386	helicase activity	2	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0008094	ATP-dependent activity, acting on DNA	2	12
GO:0016462	pyrophosphatase activity	5	12
GO:0016787	hydrolase activity	2	12
GO:0016817	hydrolase activity, acting on acid anhydrides	3	12
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	12
GO:0016887	ATP hydrolysis activity	7	12
GO:0017076	purine nucleotide binding	4	12
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140097	catalytic activity, acting on DNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0140657	ATP-dependent activity	1	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	144	148	PF00656	0.471
CLV_NRD_NRD_1	120	122	PF00675	0.271
CLV_PCSK_KEX2_1	120	122	PF00082	0.271
CLV_PCSK_KEX2_1	204	206	PF00082	0.271
CLV_PCSK_PC1ET2_1	204	206	PF00082	0.271
CLV_PCSK_SKI1_1	171	175	PF00082	0.289
CLV_PCSK_SKI1_1	424	428	PF00082	0.210
CLV_PCSK_SKI1_1	67	71	PF00082	0.271
CLV_PCSK_SKI1_1	79	83	PF00082	0.271
DEG_APCC_KENBOX_2	127	131	PF00400	0.532
DEG_SPOP_SBC_1	9	13	PF00917	0.587
DOC_ANK_TNKS_1	379	386	PF00023	0.471
DOC_CKS1_1	411	416	PF01111	0.483
DOC_CYCLIN_RxL_1	21	32	PF00134	0.471
DOC_CYCLIN_yCln2_LP_2	411	417	PF00134	0.483
DOC_MAPK_gen_1	120	127	PF00069	0.471
DOC_MAPK_gen_1	165	175	PF00069	0.471
DOC_MAPK_gen_1	47	56	PF00069	0.472
DOC_MAPK_MEF2A_6	120	127	PF00069	0.471
DOC_MAPK_MEF2A_6	155	164	PF00069	0.483
DOC_MAPK_MEF2A_6	49	58	PF00069	0.471
DOC_MAPK_MEF2A_6	79	86	PF00069	0.471
DOC_MAPK_MEF2A_6	88	96	PF00069	0.471
DOC_MAPK_NFAT4_5	120	128	PF00069	0.471
DOC_MAPK_NFAT4_5	79	87	PF00069	0.471
DOC_PP1_RVXF_1	65	72	PF00149	0.471
DOC_PP4_FxxP_1	71	74	PF00568	0.471
DOC_SPAK_OSR1_1	406	410	PF12202	0.410
DOC_USP7_MATH_1	19	23	PF00917	0.464
DOC_USP7_MATH_1	251	255	PF00917	0.576
DOC_USP7_MATH_1	321	325	PF00917	0.471
DOC_USP7_UBL2_3	284	288	PF12436	0.471
DOC_WW_Pin1_4	410	415	PF00397	0.471
LIG_14-3-3_CanoR_1	205	213	PF00244	0.471
LIG_14-3-3_CanoR_1	268	278	PF00244	0.557
LIG_Actin_RPEL_3	399	418	PF02755	0.471
LIG_Actin_WH2_2	303	321	PF00022	0.471
LIG_Actin_WH2_2	392	408	PF00022	0.471
LIG_BIR_II_1	1	5	PF00653	0.591
LIG_BRCT_BRCA1_1	403	407	PF00533	0.576
LIG_Clathr_ClatBox_1	172	176	PF01394	0.576
LIG_FHA_1	110	116	PF00498	0.471
LIG_FHA_1	273	279	PF00498	0.471
LIG_FHA_1	33	39	PF00498	0.563
LIG_FHA_1	352	358	PF00498	0.480
LIG_FHA_1	372	378	PF00498	0.191
LIG_FHA_1	411	417	PF00498	0.472
LIG_FHA_2	11	17	PF00498	0.650
LIG_FHA_2	240	246	PF00498	0.471
LIG_GBD_Chelix_1	82	90	PF00786	0.283
LIG_Integrin_isoDGR_2	422	424	PF01839	0.280
LIG_KLC1_Yacidic_2	192	197	PF13176	0.471
LIG_LIR_Gen_1	313	322	PF02991	0.471
LIG_LIR_Nem_3	221	226	PF02991	0.471
LIG_LIR_Nem_3	313	318	PF02991	0.471
LIG_NRBOX	356	362	PF00104	0.471
LIG_PCNA_yPIPBox_3	277	290	PF02747	0.471
LIG_SH2_GRB2like	317	320	PF00017	0.557
LIG_SH2_PTP2	223	226	PF00017	0.462
LIG_SH2_SRC	195	198	PF00017	0.471
LIG_SH2_SRC	223	226	PF00017	0.532
LIG_SH2_STAP1	105	109	PF00017	0.483
LIG_SH2_STAT3	289	292	PF00017	0.471
LIG_SH2_STAT3	456	459	PF00017	0.503
LIG_SH2_STAT5	195	198	PF00017	0.471
LIG_SH2_STAT5	212	215	PF00017	0.446
LIG_SH2_STAT5	223	226	PF00017	0.443
LIG_SH2_STAT5	289	292	PF00017	0.471
LIG_SH2_STAT5	317	320	PF00017	0.557
LIG_SH2_STAT5	456	459	PF00017	0.503
LIG_SH3_3	221	227	PF00018	0.471
LIG_SH3_3	89	95	PF00018	0.472
LIG_SUMO_SIM_anti_2	258	265	PF11976	0.483
LIG_SUMO_SIM_anti_2	358	365	PF11976	0.471
LIG_SUMO_SIM_anti_2	428	437	PF11976	0.501
LIG_SUMO_SIM_par_1	160	166	PF11976	0.475
LIG_SUMO_SIM_par_1	308	313	PF11976	0.471
LIG_SUMO_SIM_par_1	358	365	PF11976	0.471
LIG_TRAF2_2	253	258	PF00917	0.471
LIG_UBA3_1	161	168	PF00899	0.557
MOD_CK1_1	261	267	PF00069	0.543
MOD_CK1_1	371	377	PF00069	0.419
MOD_CK2_1	10	16	PF00069	0.649
MOD_CK2_1	372	378	PF00069	0.413
MOD_GlcNHglycan	1	4	PF01048	0.578
MOD_GlcNHglycan	105	108	PF01048	0.268
MOD_GlcNHglycan	199	202	PF01048	0.313
MOD_GlcNHglycan	444	447	PF01048	0.426
MOD_GSK3_1	163	170	PF00069	0.471
MOD_GSK3_1	258	265	PF00069	0.471
MOD_GSK3_1	331	338	PF00069	0.471
MOD_GSK3_1	368	375	PF00069	0.527
MOD_GSK3_1	99	106	PF00069	0.471
MOD_NEK2_1	322	327	PF00069	0.471
MOD_NEK2_1	58	63	PF00069	0.471
MOD_PIKK_1	163	169	PF00454	0.557
MOD_PIKK_1	251	257	PF00454	0.576
MOD_PKA_2	335	341	PF00069	0.471
MOD_Plk_1	239	245	PF00069	0.471
MOD_Plk_1	322	328	PF00069	0.471
MOD_Plk_1	371	377	PF00069	0.407
MOD_Plk_1	400	406	PF00069	0.506
MOD_Plk_2-3	186	192	PF00069	0.483
MOD_Plk_2-3	372	378	PF00069	0.401
MOD_Plk_4	258	264	PF00069	0.471
MOD_Plk_4	372	378	PF00069	0.409
MOD_ProDKin_1	410	416	PF00069	0.471
MOD_SUMO_for_1	4	7	PF00179	0.581
MOD_SUMO_rev_2	102	112	PF00179	0.471
MOD_SUMO_rev_2	437	445	PF00179	0.481
TRG_DiLeu_BaEn_1	430	435	PF01217	0.483
TRG_DiLeu_BaLyEn_6	356	361	PF01217	0.471
TRG_ENDOCYTIC_2	180	183	PF00928	0.471
TRG_ENDOCYTIC_2	223	226	PF00928	0.462
TRG_ER_diArg_1	119	121	PF00400	0.471
TRG_NES_CRM1_1	378	390	PF08389	0.455
TRG_Pf-PMV_PEXEL_1	447	451	PF00026	0.574

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I796	Leptomonas seymouri	44%	95%
A0A0N1IKW9	Leptomonas seymouri	92%	100%
A0A0S4JTM7	Bodo saltans	43%	97%
A0A0S4KMK6	Bodo saltans	82%	100%
A0A1X0P9Z8	Trypanosomatidae	86%	100%
A0A1X0PAP4	Trypanosomatidae	44%	97%
A0A3Q8IEM3	Leishmania donovani	99%	100%
A0A3Q8IFN2	Leishmania donovani	43%	95%
A0A422NEN7	Trypanosoma rangeli	86%	100%
A0A422NS36	Trypanosoma rangeli	44%	97%
A4HAX8	Leishmania braziliensis	43%	95%
A4HB68	Leishmania braziliensis	98%	100%
A4IA37	Leishmania infantum	43%	95%
A4IAB6	Leishmania infantum	99%	100%
C9ZLY0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	85%	100%
C9ZM62	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	97%
E9B552	Leishmania mexicana (strain MHOM/GT/2001/U1103)	43%	95%
E9B5D9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
O17607	Caenorhabditis elegans	59%	96%
O94692	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	44%	99%
P0CR26	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	61%	95%
P0CR27	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	61%	95%
P0CR28	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	44%	99%
P0CR29	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	44%	99%
P60122	Mus musculus	70%	100%
P60123	Rattus norvegicus	70%	100%
P83571	Danio rerio	44%	99%
Q03940	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	65%	99%
Q0IFL2	Aedes aegypti	68%	100%
Q12464	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	43%	97%
Q16TA2	Aedes aegypti	44%	99%
Q29AK9	Drosophila pseudoobscura pseudoobscura	66%	100%
Q29DI0	Drosophila pseudoobscura pseudoobscura	44%	96%
Q2TBU9	Bos taurus	44%	99%
Q4I948	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	43%	97%
Q4ICA8	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	63%	100%
Q4P112	Ustilago maydis (strain 521 / FGSC 9021)	64%	94%
Q4P6N7	Ustilago maydis (strain 521 / FGSC 9021)	45%	96%
Q4Q2T4	Leishmania major	43%	95%
Q4WKH9	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	40%	98%
Q4WPW8	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	65%	100%
Q54UW5	Dictyostelium discoideum	43%	98%
Q5A0W7	Candida albicans (strain SC5314 / ATCC MYA-2876)	64%	100%
Q5AGZ9	Candida albicans (strain SC5314 / ATCC MYA-2876)	43%	92%
Q5BBV9	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	64%	100%
Q5BGK3	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	41%	98%
Q6BI60	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	62%	100%
Q6BSB8	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	42%	96%
Q6C3X6	Yarrowia lipolytica (strain CLIB 122 / E 150)	44%	98%
Q6CB52	Yarrowia lipolytica (strain CLIB 122 / E 150)	62%	100%
Q6CQA9	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	64%	100%
Q6CT29	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	43%	98%
Q6FSF1	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	42%	96%
Q6FU78	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	64%	100%
Q750R1	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	63%	100%
Q755G5	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	42%	98%
Q873C7	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	43%	95%
Q8AWW7	Danio rerio	70%	100%
Q8STP2	Encephalitozoon cuniculi (strain GB-M1)	51%	100%
Q8SU27	Encephalitozoon cuniculi (strain GB-M1)	38%	100%
Q8WZS3	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	62%	100%
Q9C0X6	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	66%	100%
Q9DE26	Xenopus laevis	70%	100%
Q9DE27	Xenopus laevis	44%	99%
Q9FMR9	Arabidopsis thaliana	68%	100%
Q9GZH2	Caenorhabditis elegans	40%	100%
Q9V3K3	Drosophila melanogaster	44%	95%
Q9VH07	Drosophila melanogaster	66%	100%
Q9WTM5	Mus musculus	44%	99%
Q9Y230	Homo sapiens	44%	99%
Q9Y265	Homo sapiens	70%	100%
V5B579	Trypanosoma cruzi	43%	97%
V5BQJ1	Trypanosoma cruzi	86%	100%