Defective in cullin neddylation protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Cullin binding, putative

Species:

Leishmania major

UniProt:

Q4Q2B4_LEIMA

TriTrypDb:

LmjF.34.4270 , LMJLV39_340049300 , LMJSD75_340049700

Length:

236

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000151	ubiquitin ligase complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:1990234	transferase complex	3	2
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

Q4Q2B4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q2B4

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009893	positive regulation of metabolic process	4	2
GO:0010604	positive regulation of macromolecule metabolic process	5	2
GO:0019222	regulation of metabolic process	3	2
GO:0019538	protein metabolic process	3	2
GO:0031396	regulation of protein ubiquitination	8	2
GO:0031398	positive regulation of protein ubiquitination	9	2
GO:0031399	regulation of protein modification process	6	2
GO:0031401	positive regulation of protein modification process	7	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0036211	protein modification process	4	2
GO:0043085	positive regulation of catalytic activity	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044093	positive regulation of molecular function	3	2
GO:0044238	primary metabolic process	2	2
GO:0045116	protein neddylation	7	2
GO:0048518	positive regulation of biological process	3	2
GO:0050789	regulation of biological process	2	2
GO:0050790	regulation of catalytic activity	3	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051173	positive regulation of nitrogen compound metabolic process	5	2
GO:0051246	regulation of protein metabolic process	5	2
GO:0051247	positive regulation of protein metabolic process	6	2
GO:0051338	regulation of transferase activity	4	2
GO:0051347	positive regulation of transferase activity	5	2
GO:0051438	regulation of ubiquitin-protein transferase activity	5	2
GO:0051443	positive regulation of ubiquitin-protein transferase activity	6	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065007	biological regulation	1	2
GO:0065009	regulation of molecular function	2	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:0080090	regulation of primary metabolic process	4	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1903320	regulation of protein modification by small protein conjugation or removal	7	2
GO:1903322	positive regulation of protein modification by small protein conjugation or removal	8	2

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	2
GO:0005515	protein binding	2	2
GO:0019899	enzyme binding	3	2
GO:0031624	ubiquitin conjugating enzyme binding	5	2
GO:0032182	ubiquitin-like protein binding	3	2
GO:0044390	ubiquitin-like protein conjugating enzyme binding	4	2
GO:0097602	cullin family protein binding	3	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	153	155	PF00675	0.419
CLV_PCSK_KEX2_1	153	155	PF00082	0.433
CLV_PCSK_SKI1_1	153	157	PF00082	0.336
DEG_Nend_UBRbox_1	1	4	PF02207	0.597
DOC_MAPK_gen_1	37	47	PF00069	0.507
DOC_MAPK_gen_1	6	13	PF00069	0.651
DOC_MAPK_MEF2A_6	195	203	PF00069	0.237
DOC_MAPK_MEF2A_6	40	47	PF00069	0.461
DOC_MAPK_NFAT4_5	40	48	PF00069	0.470
DOC_USP7_MATH_1	208	212	PF00917	0.447
DOC_USP7_MATH_1	219	223	PF00917	0.411
DOC_USP7_MATH_1	22	26	PF00917	0.491
DOC_USP7_MATH_1	9	13	PF00917	0.643
LIG_14-3-3_CanoR_1	119	129	PF00244	0.312
LIG_14-3-3_CanoR_1	153	158	PF00244	0.412
LIG_14-3-3_CanoR_1	6	12	PF00244	0.578
LIG_BRCT_BRCA1_1	9	13	PF00533	0.539
LIG_deltaCOP1_diTrp_1	192	200	PF00928	0.257
LIG_EH1_1	109	117	PF00400	0.449
LIG_FHA_1	156	162	PF00498	0.337
LIG_FHA_2	97	103	PF00498	0.343
LIG_LIR_Gen_1	135	144	PF02991	0.296
LIG_LIR_Gen_1	44	53	PF02991	0.377
LIG_LIR_Gen_1	78	87	PF02991	0.226
LIG_LIR_Nem_3	192	197	PF02991	0.257
LIG_LIR_Nem_3	44	50	PF02991	0.327
LIG_Pex14_1	164	168	PF04695	0.272
LIG_PTB_Apo_2	138	145	PF02174	0.293
LIG_PTB_Phospho_1	138	144	PF10480	0.293
LIG_REV1ctd_RIR_1	165	174	PF16727	0.287
LIG_SH2_CRK	140	144	PF00017	0.296
LIG_SH2_SRC	108	111	PF00017	0.419
LIG_SH2_SRC	144	147	PF00017	0.306
LIG_SH2_STAT5	142	145	PF00017	0.366
LIG_SH2_STAT5	146	149	PF00017	0.336
LIG_TYR_ITIM	138	143	PF00017	0.409
LIG_UBA3_1	86	95	PF00899	0.332
MOD_CK1_1	132	138	PF00069	0.371
MOD_CK1_1	56	62	PF00069	0.524
MOD_CK2_1	38	44	PF00069	0.396
MOD_CK2_1	76	82	PF00069	0.398
MOD_CK2_1	96	102	PF00069	0.363
MOD_CMANNOS	177	180	PF00535	0.257
MOD_GlcNHglycan	122	125	PF01048	0.256
MOD_GlcNHglycan	131	134	PF01048	0.469
MOD_GlcNHglycan	210	213	PF01048	0.308
MOD_GlcNHglycan	215	218	PF01048	0.306
MOD_GlcNHglycan	221	224	PF01048	0.305
MOD_GlcNHglycan	25	28	PF01048	0.606
MOD_GlcNHglycan	54	58	PF01048	0.552
MOD_GlcNHglycan	62	65	PF01048	0.522
MOD_GSK3_1	18	25	PF00069	0.560
MOD_GSK3_1	199	206	PF00069	0.383
MOD_GSK3_1	56	63	PF00069	0.517
MOD_N-GLC_1	120	125	PF02516	0.262
MOD_NEK2_1	155	160	PF00069	0.334
MOD_NEK2_1	203	208	PF00069	0.353
MOD_NEK2_1	53	58	PF00069	0.553
MOD_NEK2_1	7	12	PF00069	0.473
MOD_NEK2_1	90	95	PF00069	0.329
MOD_NEK2_2	103	108	PF00069	0.361
MOD_PIKK_1	90	96	PF00454	0.409
MOD_PKA_1	153	159	PF00069	0.337
MOD_PKA_2	129	135	PF00069	0.405
MOD_PKA_2	153	159	PF00069	0.388
MOD_PKA_2	7	13	PF00069	0.622
MOD_PKB_1	15	23	PF00069	0.630
MOD_Plk_1	120	126	PF00069	0.356
MOD_Plk_1	76	82	PF00069	0.363
MOD_Plk_2-3	77	83	PF00069	0.371
MOD_Plk_4	103	109	PF00069	0.360
MOD_Plk_4	111	117	PF00069	0.402
MOD_Plk_4	132	138	PF00069	0.375
TRG_DiLeu_BaEn_1	82	87	PF01217	0.314
TRG_ENDOCYTIC_2	140	143	PF00928	0.287
TRG_ENDOCYTIC_2	144	147	PF00928	0.301
TRG_ER_diArg_1	153	155	PF00400	0.438
TRG_NES_CRM1_1	192	204	PF08389	0.399
TRG_Pf-PMV_PEXEL_1	191	196	PF00026	0.317

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I8Z2	Leptomonas seymouri	50%	98%
A0A0S4KLZ2	Bodo saltans	38%	81%
A0A1X0PA72	Trypanosomatidae	35%	96%
A0A3R7K285	Trypanosoma rangeli	36%	86%
A0A3S7X8J3	Leishmania donovani	92%	100%
A4HBF0	Leishmania braziliensis	69%	97%
A4IAJ8	Leishmania infantum	92%	100%
C9ZLP3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
E9B5M2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	100%
Q12395	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	22%	88%
Q54GP1	Dictyostelium discoideum	21%	95%
Q5AWS1	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	23%	77%
Q5RHX6	Danio rerio	24%	84%
Q5ZKU1	Gallus gallus	21%	91%
Q6C0B6	Yarrowia lipolytica (strain CLIB 122 / E 150)	23%	98%
Q6PH85	Homo sapiens	21%	91%
Q8BZJ7	Mus musculus	20%	91%
Q8CCA0	Mus musculus	22%	81%
Q92564	Homo sapiens	22%	81%
Q96GG9	Homo sapiens	21%	91%
Q9QZ73	Mus musculus	21%	91%
Q9U3C8	Caenorhabditis elegans	21%	80%
Q9VUQ8	Drosophila melanogaster	23%	82%
V5BLR0	Trypanosoma cruzi	36%	94%