Putative PUF1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Putative PUF1

Gene product:

PUF1, putative

Species:

Leishmania major

UniProt:

Q4Q274_LEIMA

TriTrypDb:

LmjF.36.0050 , LMJLV39_360005400 , LMJSD75_360005400

Length:

553

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 13
NetGPI	no	yes: 0, no: 14

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

Q4Q274

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q274

Function

Biological processes
Term	Name	Level	Count
GO:0010468	regulation of gene expression	5	2
GO:0010608	post-transcriptional regulation of gene expression	6	2
GO:0019222	regulation of metabolic process	3	2
GO:0050789	regulation of biological process	2	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065007	biological regulation	1	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	15
GO:0003723	RNA binding	4	15
GO:0003729	mRNA binding	5	2
GO:0005488	binding	1	15
GO:0097159	organic cyclic compound binding	2	15
GO:1901363	heterocyclic compound binding	2	15

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	13	15	PF00675	0.461
CLV_NRD_NRD_1	442	444	PF00675	0.453
CLV_NRD_NRD_1	449	451	PF00675	0.489
CLV_PCSK_KEX2_1	13	15	PF00082	0.477
CLV_PCSK_KEX2_1	44	46	PF00082	0.596
CLV_PCSK_KEX2_1	442	444	PF00082	0.455
CLV_PCSK_KEX2_1	449	451	PF00082	0.503
CLV_PCSK_PC1ET2_1	44	46	PF00082	0.650
CLV_PCSK_PC7_1	445	451	PF00082	0.581
CLV_PCSK_SKI1_1	104	108	PF00082	0.392
CLV_PCSK_SKI1_1	113	117	PF00082	0.443
CLV_PCSK_SKI1_1	140	144	PF00082	0.463
CLV_PCSK_SKI1_1	224	228	PF00082	0.470
CLV_PCSK_SKI1_1	343	347	PF00082	0.391
CLV_PCSK_SKI1_1	364	368	PF00082	0.323
CLV_PCSK_SKI1_1	374	378	PF00082	0.283
CLV_PCSK_SKI1_1	389	393	PF00082	0.383
CLV_PCSK_SKI1_1	449	453	PF00082	0.619
CLV_PCSK_SKI1_1	69	73	PF00082	0.487
CLV_PCSK_SKI1_1	75	79	PF00082	0.472
DEG_APCC_DBOX_1	342	350	PF00400	0.384
DEG_APCC_DBOX_1	74	82	PF00400	0.479
DOC_CYCLIN_RxL_1	443	456	PF00134	0.520
DOC_CYCLIN_yCln2_LP_2	472	475	PF00134	0.664
DOC_PP2B_LxvP_1	381	384	PF13499	0.510
DOC_PP2B_LxvP_1	472	475	PF13499	0.720
DOC_PP4_FxxP_1	148	151	PF00568	0.479
DOC_PP4_FxxP_1	510	513	PF00568	0.714
DOC_USP7_MATH_1	122	126	PF00917	0.412
DOC_USP7_MATH_1	182	186	PF00917	0.408
DOC_USP7_MATH_1	422	426	PF00917	0.523
DOC_USP7_MATH_1	490	494	PF00917	0.665
DOC_USP7_UBL2_3	435	439	PF12436	0.400
DOC_WW_Pin1_4	367	372	PF00397	0.372
DOC_WW_Pin1_4	427	432	PF00397	0.426
LIG_14-3-3_CanoR_1	140	145	PF00244	0.493
LIG_14-3-3_CanoR_1	183	191	PF00244	0.396
LIG_Actin_WH2_2	284	299	PF00022	0.361
LIG_Actin_WH2_2	354	369	PF00022	0.286
LIG_Actin_WH2_2	413	430	PF00022	0.431
LIG_Actin_WH2_2	61	77	PF00022	0.412
LIG_AP2alpha_2	301	303	PF02296	0.286
LIG_BIR_II_1	1	5	PF00653	0.557
LIG_BIR_III_4	121	125	PF00653	0.462
LIG_BIR_III_4	70	74	PF00653	0.443
LIG_BRCT_BRCA1_1	227	231	PF00533	0.480
LIG_BRCT_BRCA1_1	26	30	PF00533	0.488
LIG_CaM_IQ_9	84	100	PF13499	0.531
LIG_EVH1_2	535	539	PF00568	0.668
LIG_FHA_1	137	143	PF00498	0.474
LIG_FHA_1	200	206	PF00498	0.460
LIG_FHA_1	283	289	PF00498	0.335
LIG_FHA_1	336	342	PF00498	0.381
LIG_FHA_1	539	545	PF00498	0.681
LIG_FHA_2	141	147	PF00498	0.539
LIG_FHA_2	150	156	PF00498	0.506
LIG_FHA_2	192	198	PF00498	0.353
LIG_FHA_2	404	410	PF00498	0.353
LIG_FHA_2	44	50	PF00498	0.612
LIG_LIR_Apic_2	146	151	PF02991	0.480
LIG_LIR_Apic_2	509	513	PF02991	0.726
LIG_LIR_Gen_1	199	209	PF02991	0.449
LIG_LIR_Gen_1	228	238	PF02991	0.516
LIG_LIR_Gen_1	249	260	PF02991	0.395
LIG_LIR_Gen_1	499	508	PF02991	0.679
LIG_LIR_Nem_3	196	201	PF02991	0.431
LIG_LIR_Nem_3	228	234	PF02991	0.491
LIG_LIR_Nem_3	249	255	PF02991	0.380
LIG_LIR_Nem_3	301	306	PF02991	0.337
LIG_LIR_Nem_3	392	398	PF02991	0.287
LIG_LIR_Nem_3	499	503	PF02991	0.653
LIG_MYND_1	471	475	PF01753	0.728
LIG_PCNA_yPIPBox_3	214	226	PF02747	0.368
LIG_PCNA_yPIPBox_3	504	515	PF02747	0.648
LIG_Pex14_2	103	107	PF04695	0.390
LIG_PTB_Apo_2	426	433	PF02174	0.395
LIG_PTB_Phospho_1	426	432	PF10480	0.399
LIG_SH2_CRK	201	205	PF00017	0.461
LIG_SH2_CRK	476	480	PF00017	0.721
LIG_SH2_GRB2like	337	340	PF00017	0.393
LIG_SH2_NCK_1	248	252	PF00017	0.430
LIG_SH2_STAP1	201	205	PF00017	0.461
LIG_SH2_STAP1	248	252	PF00017	0.451
LIG_SH2_STAP1	337	341	PF00017	0.418
LIG_SH2_STAT3	306	309	PF00017	0.286
LIG_SH2_STAT3	337	340	PF00017	0.468
LIG_SH2_STAT3	398	401	PF00017	0.286
LIG_SH2_STAT5	201	204	PF00017	0.436
LIG_SH2_STAT5	306	309	PF00017	0.286
LIG_SH2_STAT5	337	340	PF00017	0.393
LIG_SH2_STAT5	398	401	PF00017	0.286
LIG_SH2_STAT5	86	89	PF00017	0.486
LIG_SH3_3	201	207	PF00018	0.467
LIG_SH3_3	296	302	PF00018	0.308
LIG_SH3_3	468	474	PF00018	0.696
LIG_SH3_3	529	535	PF00018	0.704
LIG_SUMO_SIM_anti_2	159	167	PF11976	0.375
LIG_SUMO_SIM_par_1	159	167	PF11976	0.370
LIG_TRAF2_1	194	197	PF00917	0.286
LIG_TRAF2_1	2	5	PF00917	0.488
LIG_TRAF2_1	467	470	PF00917	0.712
LIG_UBA3_1	420	428	PF00899	0.434
LIG_WRC_WIRS_1	507	512	PF05994	0.726
MOD_CDK_SPxxK_3	367	374	PF00069	0.382
MOD_CK1_1	237	243	PF00069	0.582
MOD_CK1_1	405	411	PF00069	0.352
MOD_CK1_1	438	444	PF00069	0.521
MOD_CK1_1	538	544	PF00069	0.560
MOD_CK2_1	149	155	PF00069	0.545
MOD_CK2_1	191	197	PF00069	0.286
MOD_CK2_1	200	206	PF00069	0.328
MOD_CK2_1	43	49	PF00069	0.588
MOD_GlcNHglycan	136	139	PF01048	0.504
MOD_GlcNHglycan	227	230	PF01048	0.510
MOD_GlcNHglycan	238	242	PF01048	0.532
MOD_GlcNHglycan	482	485	PF01048	0.735
MOD_GlcNHglycan	51	54	PF01048	0.605
MOD_GlcNHglycan	546	549	PF01048	0.704
MOD_GSK3_1	136	143	PF00069	0.488
MOD_GSK3_1	167	174	PF00069	0.433
MOD_GSK3_1	19	26	PF00069	0.473
MOD_GSK3_1	220	227	PF00069	0.481
MOD_GSK3_1	283	290	PF00069	0.377
MOD_GSK3_1	30	37	PF00069	0.490
MOD_GSK3_1	399	406	PF00069	0.311
MOD_GSK3_1	498	505	PF00069	0.646
MOD_N-GLC_1	122	127	PF02516	0.396
MOD_N-GLC_1	218	223	PF02516	0.378
MOD_N-GLC_1	292	297	PF02516	0.496
MOD_N-GLC_1	335	340	PF02516	0.366
MOD_N-GLC_1	49	54	PF02516	0.668
MOD_N-GLC_1	544	549	PF02516	0.690
MOD_NEK2_1	134	139	PF00069	0.469
MOD_NEK2_1	225	230	PF00069	0.444
MOD_NEK2_1	292	297	PF00069	0.383
MOD_NEK2_1	366	371	PF00069	0.423
MOD_NEK2_1	391	396	PF00069	0.330
MOD_PIKK_1	182	188	PF00454	0.396
MOD_PIKK_1	411	417	PF00454	0.343
MOD_PIKK_1	459	465	PF00454	0.657
MOD_PKA_2	182	188	PF00069	0.394
MOD_Plk_1	218	224	PF00069	0.436
MOD_Plk_1	24	30	PF00069	0.563
MOD_Plk_1	292	298	PF00069	0.476
MOD_Plk_1	490	496	PF00069	0.710
MOD_Plk_4	200	206	PF00069	0.414
MOD_Plk_4	283	289	PF00069	0.326
MOD_Plk_4	422	428	PF00069	0.431
MOD_Plk_4	506	512	PF00069	0.635
MOD_ProDKin_1	367	373	PF00069	0.372
MOD_ProDKin_1	427	433	PF00069	0.420
MOD_SUMO_for_1	321	324	PF00179	0.454
MOD_SUMO_for_1	434	437	PF00179	0.437
MOD_SUMO_rev_2	2	8	PF00179	0.584
MOD_SUMO_rev_2	48	52	PF00179	0.678
TRG_DiLeu_BaEn_1	160	165	PF01217	0.349
TRG_DiLeu_BaLyEn_6	130	135	PF01217	0.507
TRG_DiLeu_BaLyEn_6	447	452	PF01217	0.524
TRG_DiLeu_BaLyEn_6	468	473	PF01217	0.667
TRG_ENDOCYTIC_2	201	204	PF00928	0.464
TRG_ENDOCYTIC_2	248	251	PF00928	0.355
TRG_ER_diArg_1	442	445	PF00400	0.484
TRG_ER_diArg_1	449	451	PF00400	0.532
TRG_Pf-PMV_PEXEL_1	166	171	PF00026	0.498
TRG_Pf-PMV_PEXEL_1	19	23	PF00026	0.430
TRG_Pf-PMV_PEXEL_1	374	379	PF00026	0.314
TRG_Pf-PMV_PEXEL_1	45	49	PF00026	0.656

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P473	Leptomonas seymouri	80%	99%
A0A0S4IY52	Bodo saltans	29%	78%
A0A0S4JMB8	Bodo saltans	46%	79%
A0A1X0NHM5	Trypanosomatidae	29%	98%
A0A1X0P8D8	Trypanosomatidae	46%	96%
A0A3Q8IK20	Leishmania donovani	98%	100%
A0A3R7NHA6	Trypanosoma rangeli	45%	97%
A0A422N8V0	Trypanosoma rangeli	28%	100%
A4HNL0	Leishmania braziliensis	89%	100%
A4ICY3	Leishmania infantum	99%	100%
C9ZII7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	82%
D0A2G7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	97%
E9ASC2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
E9AUS6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	100%
Q4QCQ8	Leishmania major	24%	100%
Q9C9R6	Arabidopsis thaliana	28%	85%
V5B1W9	Trypanosoma cruzi	29%	99%
V5BV90	Trypanosoma cruzi	46%	91%