Putative dolicholphosphate-mannose synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

dolicholphosphate-mannose synthase, putative

Species:

Leishmania major

UniProt:

Q4Q247_LEIMA

TriTrypDb:

LmjF.36.0310 , LMJLV39_360008400 , LMJSD75_360008200

Length:

230

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 3

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 12

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000814	ESCRT II complex	3	12
GO:0005654	nucleoplasm	2	2
GO:0005737	cytoplasm	2	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0032991	protein-containing complex	1	12
GO:0036452	ESCRT complex	2	12
GO:0097014	ciliary plasm	5	2
GO:0098796	membrane protein complex	2	12
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q247

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q247

Function

Biological processes
Term	Name	Level	Count
GO:0006488	dolichol-linked oligosaccharide biosynthetic process	5	2
GO:0006490	oligosaccharide-lipid intermediate biosynthetic process	4	2
GO:0006629	lipid metabolic process	3	2
GO:0006810	transport	3	12
GO:0006886	intracellular protein transport	4	2
GO:0007034	vacuolar transport	4	2
GO:0008104	protein localization	4	2
GO:0008152	metabolic process	1	2
GO:0009058	biosynthetic process	2	2
GO:0009987	cellular process	1	2
GO:0015031	protein transport	4	2
GO:0016192	vesicle-mediated transport	4	12
GO:0016197	endosomal transport	4	2
GO:0032509	endosome transport via multivesicular body sorting pathway	5	2
GO:0032511	late endosome to vacuole transport via multivesicular body sorting pathway	6	2
GO:0033036	macromolecule localization	2	2
GO:0033365	protein localization to organelle	5	2
GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044255	cellular lipid metabolic process	3	2
GO:0045184	establishment of protein localization	3	2
GO:0045324	late endosome to vacuole transport	5	2
GO:0046907	intracellular transport	3	2
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0070727	cellular macromolecule localization	3	2
GO:0071702	organic substance transport	4	2
GO:0071704	organic substance metabolic process	2	2
GO:0071705	nitrogen compound transport	4	2
GO:0071985	multivesicular body sorting pathway	5	12
GO:0072594	establishment of protein localization to organelle	4	2
GO:0072665	protein localization to vacuole	6	2
GO:0072666	establishment of protein localization to vacuole	5	2
GO:1901135	carbohydrate derivative metabolic process	3	2
GO:1901137	carbohydrate derivative biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0000030	mannosyltransferase activity	5	5
GO:0003824	catalytic activity	1	5
GO:0004582	dolichyl-phosphate beta-D-mannosyltransferase activity	6	5
GO:0005198	structural molecule activity	1	2
GO:0005488	binding	1	2
GO:0005515	protein binding	2	2
GO:0016740	transferase activity	2	5
GO:0016757	glycosyltransferase activity	3	5
GO:0016758	hexosyltransferase activity	4	5
GO:0042802	identical protein binding	3	2
GO:0042803	protein homodimerization activity	4	2
GO:0046983	protein dimerization activity	3	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	198	200	PF00675	0.427
CLV_PCSK_KEX2_1	198	200	PF00082	0.427
CLV_PCSK_KEX2_1	72	74	PF00082	0.313
CLV_PCSK_PC1ET2_1	72	74	PF00082	0.378
CLV_PCSK_SKI1_1	125	129	PF00082	0.523
CLV_PCSK_SKI1_1	205	209	PF00082	0.599
DEG_APCC_DBOX_1	189	197	PF00400	0.433
DOC_ANK_TNKS_1	72	79	PF00023	0.575
DOC_MAPK_gen_1	141	148	PF00069	0.567
DOC_PP4_FxxP_1	65	68	PF00568	0.550
DOC_PP4_FxxP_1	9	12	PF00568	0.377
DOC_WW_Pin1_4	72	77	PF00397	0.595
LIG_14-3-3_CanoR_1	169	177	PF00244	0.491
LIG_14-3-3_CanoR_1	182	189	PF00244	0.389
LIG_14-3-3_CanoR_1	198	207	PF00244	0.408
LIG_14-3-3_CanoR_1	26	31	PF00244	0.534
LIG_14-3-3_CanoR_1	56	66	PF00244	0.507
LIG_Actin_WH2_2	112	127	PF00022	0.623
LIG_BIR_II_1	1	5	PF00653	0.470
LIG_BRCT_BRCA1_1	61	65	PF00533	0.509
LIG_Clathr_ClatBox_1	211	215	PF01394	0.478
LIG_deltaCOP1_diTrp_1	4	9	PF00928	0.477
LIG_FHA_1	134	140	PF00498	0.500
LIG_FHA_1	49	55	PF00498	0.542
LIG_FHA_2	109	115	PF00498	0.623
LIG_FHA_2	145	151	PF00498	0.451
LIG_FHA_2	58	64	PF00498	0.614
LIG_LIR_Apic_2	62	68	PF02991	0.550
LIG_LIR_Gen_1	104	109	PF02991	0.553
LIG_LIR_Gen_1	147	156	PF02991	0.445
LIG_LIR_Gen_1	29	39	PF02991	0.570
LIG_LIR_Gen_1	3	14	PF02991	0.466
LIG_LIR_Nem_3	104	108	PF02991	0.550
LIG_LIR_Nem_3	147	151	PF02991	0.441
LIG_LIR_Nem_3	219	225	PF02991	0.546
LIG_LIR_Nem_3	29	34	PF02991	0.550
LIG_LIR_Nem_3	3	9	PF02991	0.447
LIG_SH2_CRK	222	226	PF00017	0.546
LIG_SH2_SRC	192	195	PF00017	0.541
LIG_SH2_STAT3	41	44	PF00017	0.587
LIG_SH2_STAT3	58	61	PF00017	0.559
LIG_SH2_STAT5	126	129	PF00017	0.535
LIG_SH2_STAT5	166	169	PF00017	0.572
LIG_SH2_STAT5	192	195	PF00017	0.541
LIG_SH2_STAT5	58	61	PF00017	0.544
LIG_SUMO_SIM_par_1	93	99	PF11976	0.448
LIG_TRFH_1	9	13	PF08558	0.362
LIG_WRC_WIRS_1	145	150	PF05994	0.448
MOD_CK1_1	20	26	PF00069	0.595
MOD_CK2_1	144	150	PF00069	0.444
MOD_GlcNHglycan	152	155	PF01048	0.598
MOD_GlcNHglycan	163	166	PF01048	0.578
MOD_GlcNHglycan	171	174	PF01048	0.554
MOD_GlcNHglycan	20	23	PF01048	0.326
MOD_GlcNHglycan	201	204	PF01048	0.594
MOD_GSK3_1	13	20	PF00069	0.556
MOD_GSK3_1	133	140	PF00069	0.560
MOD_GSK3_1	78	85	PF00069	0.552
MOD_NEK2_1	119	124	PF00069	0.515
MOD_NEK2_1	207	212	PF00069	0.534
MOD_NEK2_1	214	219	PF00069	0.541
MOD_NEK2_1	57	62	PF00069	0.521
MOD_NEK2_1	82	87	PF00069	0.534
MOD_PIKK_1	216	222	PF00454	0.613
MOD_PIKK_1	57	63	PF00454	0.559
MOD_PK_1	103	109	PF00069	0.614
MOD_PKA_1	141	147	PF00069	0.573
MOD_PKA_2	168	174	PF00069	0.535
MOD_PKA_2	181	187	PF00069	0.404
MOD_Plk_1	103	109	PF00069	0.574
MOD_Plk_1	119	125	PF00069	0.381
MOD_Plk_4	119	125	PF00069	0.447
MOD_Plk_4	141	147	PF00069	0.579
MOD_Plk_4	207	213	PF00069	0.515
MOD_Plk_4	26	32	PF00069	0.534
MOD_Plk_4	78	84	PF00069	0.543
MOD_ProDKin_1	72	78	PF00069	0.595
TRG_ENDOCYTIC_2	222	225	PF00928	0.457
TRG_ER_diArg_1	198	200	PF00400	0.620
TRG_Pf-PMV_PEXEL_1	125	129	PF00026	0.553

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I8X1	Leptomonas seymouri	81%	99%
A0A0S4JPF6	Bodo saltans	32%	89%
A0A1X0P8B3	Trypanosomatidae	49%	98%
A0A3R7MAA3	Trypanosoma rangeli	50%	95%
A0A3S7XAA6	Leishmania donovani	96%	100%
A4HNN9	Leishmania braziliensis	88%	100%
A4ICV6	Leishmania infantum	96%	100%
D0A2J3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	98%
E9ASE8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
V5BV71	Trypanosoma cruzi	51%	96%