Putative small nuclear ribonucleaoprotein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

U2 small nuclear ribonucleoprotein 16.5K, putative

Species:

Leishmania major

UniProt:

Q4Q224_LEIMA

TriTrypDb:

LmjF.36.0535 , LMJLV39_360010800 , LMJSD75_360010700

Length:

141

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0000243	commitment complex	5	2
GO:0005634	nucleus	5	5
GO:0005681	spliceosomal complex	3	2
GO:0005682	U5 snRNP	5	2
GO:0005684	U2-type spliceosomal complex	4	2
GO:0005685	U1 snRNP	5	2
GO:0005686	U2 snRNP	5	2
GO:0005687	U4 snRNP	5	2
GO:0005689	U12-type spliceosomal complex	4	2
GO:0030532	small nuclear ribonucleoprotein complex	3	2
GO:0032991	protein-containing complex	1	8
GO:0034715	pICln-Sm protein complex	3	2
GO:0034719	SMN-Sm protein complex	3	2
GO:0043226	organelle	2	5
GO:0043227	membrane-bounded organelle	3	5
GO:0043229	intracellular organelle	3	5
GO:0043231	intracellular membrane-bounded organelle	4	5
GO:0071011	precatalytic spliceosome	4	2
GO:0071013	catalytic step 2 spliceosome	3	2
GO:0097525	spliceosomal snRNP complex	4	2
GO:0097526	spliceosomal tri-snRNP complex	5	2
GO:0110165	cellular anatomical entity	1	5
GO:0120114	Sm-like protein family complex	2	2
GO:0140513	nuclear protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:1990904	ribonucleoprotein complex	2	8

Expansion

Sequence features

Q4Q224

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q224

Function

Biological processes
Term	Name	Level	Count
GO:0000375	RNA splicing, via transesterification reactions	8	2
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	2
GO:0000380	alternative mRNA splicing, via spliceosome	9	2
GO:0000387	spliceosomal snRNP assembly	7	2
GO:0000398	mRNA splicing, via spliceosome	8	2
GO:0006139	nucleobase-containing compound metabolic process	3	7
GO:0006396	RNA processing	6	7
GO:0006397	mRNA processing	7	2
GO:0006725	cellular aromatic compound metabolic process	3	7
GO:0006807	nitrogen compound metabolic process	2	7
GO:0008152	metabolic process	1	7
GO:0008380	RNA splicing	7	2
GO:0009987	cellular process	1	7
GO:0016043	cellular component organization	3	2
GO:0016070	RNA metabolic process	5	7
GO:0016071	mRNA metabolic process	6	2
GO:0022607	cellular component assembly	4	2
GO:0022618	ribonucleoprotein complex assembly	6	2
GO:0034641	cellular nitrogen compound metabolic process	3	7
GO:0043170	macromolecule metabolic process	3	7
GO:0043933	protein-containing complex organization	4	2
GO:0044237	cellular metabolic process	2	7
GO:0044238	primary metabolic process	2	7
GO:0046483	heterocycle metabolic process	3	7
GO:0065003	protein-containing complex assembly	5	2
GO:0071704	organic substance metabolic process	2	7
GO:0071826	ribonucleoprotein complex subunit organization	5	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:0090304	nucleic acid metabolic process	4	7
GO:1901360	organic cyclic compound metabolic process	3	7

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	2
GO:0003723	RNA binding	4	2
GO:0005488	binding	1	2
GO:0097159	organic cyclic compound binding	2	2
GO:1901363	heterocyclic compound binding	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	137	139	PF00675	0.700
CLV_NRD_NRD_1	93	95	PF00675	0.593
CLV_PCSK_KEX2_1	137	139	PF00082	0.636
CLV_PCSK_KEX2_1	93	95	PF00082	0.608
CLV_PCSK_SKI1_1	103	107	PF00082	0.574
CLV_PCSK_SKI1_1	76	80	PF00082	0.355
DOC_CDC14_PxL_1	105	113	PF14671	0.639
DOC_CDC14_PxL_1	84	92	PF14671	0.569
DOC_CYCLIN_yCln2_LP_2	79	82	PF00134	0.490
DOC_MAPK_gen_1	102	110	PF00069	0.490
DOC_MAPK_gen_1	71	80	PF00069	0.600
DOC_MAPK_MEF2A_6	71	79	PF00069	0.597
DOC_MAPK_RevD_3	109	125	PF00069	0.717
DOC_PP2B_LxvP_1	79	82	PF13499	0.490
DOC_USP7_MATH_1	19	23	PF00917	0.362
DOC_USP7_MATH_1	2	6	PF00917	0.703
DOC_USP7_MATH_1	57	61	PF00917	0.568
DOC_USP7_MATH_1	86	90	PF00917	0.560
DOC_USP7_UBL2_3	102	106	PF12436	0.496
DOC_USP7_UBL2_3	125	129	PF12436	0.745
LIG_14-3-3_CanoR_1	67	71	PF00244	0.599
LIG_Actin_WH2_2	98	114	PF00022	0.670
LIG_BIR_II_1	1	5	PF00653	0.713
LIG_BRCT_BRCA1_1	13	17	PF00533	0.540
LIG_FHA_1	6	12	PF00498	0.633
LIG_LIR_Gen_1	14	24	PF02991	0.399
LIG_LIR_Nem_3	59	65	PF02991	0.597
LIG_MYND_1	109	113	PF01753	0.653
LIG_NRP_CendR_1	138	141	PF00754	0.758
LIG_SH2_NCK_1	65	69	PF00017	0.597
LIG_SH3_3	107	113	PF00018	0.664
LIG_SH3_3	75	81	PF00018	0.518
LIG_TRAF2_1	96	99	PF00917	0.389
MOD_CK1_1	22	28	PF00069	0.514
MOD_CK1_1	5	11	PF00069	0.648
MOD_CK2_1	93	99	PF00069	0.470
MOD_GSK3_1	1	8	PF00069	0.755
MOD_GSK3_1	66	73	PF00069	0.597
MOD_N-GLC_1	5	10	PF02516	0.696
MOD_NEK2_1	1	6	PF00069	0.766
MOD_NEK2_1	101	106	PF00069	0.504
MOD_NEK2_1	41	46	PF00069	0.596
MOD_PK_1	93	99	PF00069	0.584
MOD_PKA_1	93	99	PF00069	0.545
MOD_PKA_2	22	28	PF00069	0.560
MOD_PKA_2	66	72	PF00069	0.536
MOD_PKA_2	93	99	PF00069	0.626
MOD_Plk_1	58	64	PF00069	0.501
MOD_Plk_4	11	17	PF00069	0.574
MOD_SUMO_rev_2	95	104	PF00179	0.532
TRG_ENDOCYTIC_2	65	68	PF00928	0.597
TRG_ER_diArg_1	136	138	PF00400	0.638
TRG_ER_diArg_1	92	94	PF00400	0.668

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A1X0P880	Trypanosomatidae	45%	100%
A0A3S5ISK5	Trypanosoma rangeli	38%	100%
A0A3S7XAB5	Leishmania donovani	89%	100%
A4HNR2	Leishmania braziliensis	77%	100%
A4ICT3	Leishmania infantum	89%	100%
D0A2M1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	100%
E9ASH1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%