Putative tRNA pseudouridine synthase A

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase A, putative

Species:

Leishmania major

UniProt:

Q4Q1Q8_LEIMA

TriTrypDb:

LmjF.36.1660 , LMJLV39_360023100 , LMJSD75_360022900

Length:

694

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

Q4Q1Q8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q1Q8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	7
GO:0006364	rRNA processing	8	3
GO:0006396	RNA processing	6	3
GO:0006725	cellular aromatic compound metabolic process	3	7
GO:0006807	nitrogen compound metabolic process	2	7
GO:0008152	metabolic process	1	7
GO:0009987	cellular process	1	7
GO:0016070	RNA metabolic process	5	7
GO:0016072	rRNA metabolic process	7	3
GO:0034470	ncRNA processing	7	3
GO:0034641	cellular nitrogen compound metabolic process	3	7
GO:0034660	ncRNA metabolic process	6	3
GO:0043170	macromolecule metabolic process	3	7
GO:0044237	cellular metabolic process	2	7
GO:0044238	primary metabolic process	2	7
GO:0046483	heterocycle metabolic process	3	7
GO:0071704	organic substance metabolic process	2	7
GO:0090304	nucleic acid metabolic process	4	7
GO:1901360	organic cyclic compound metabolic process	3	7
GO:0001522	pseudouridine synthesis	6	4
GO:0009451	RNA modification	5	4
GO:0043412	macromolecule modification	4	4

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0004730	pseudouridylate synthase activity	5	8
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016829	lyase activity	2	8
GO:0016835	carbon-oxygen lyase activity	3	8
GO:0016836	hydro-lyase activity	4	8
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	131	135	PF00656	0.661
CLV_C14_Caspase3-7	604	608	PF00656	0.451
CLV_C14_Caspase3-7	613	617	PF00656	0.416
CLV_NRD_NRD_1	108	110	PF00675	0.722
CLV_NRD_NRD_1	214	216	PF00675	0.762
CLV_NRD_NRD_1	255	257	PF00675	0.769
CLV_NRD_NRD_1	297	299	PF00675	0.641
CLV_NRD_NRD_1	308	310	PF00675	0.669
CLV_NRD_NRD_1	31	33	PF00675	0.586
CLV_NRD_NRD_1	346	348	PF00675	0.257
CLV_NRD_NRD_1	48	50	PF00675	0.722
CLV_NRD_NRD_1	56	58	PF00675	0.634
CLV_NRD_NRD_1	582	584	PF00675	0.408
CLV_NRD_NRD_1	590	592	PF00675	0.427
CLV_NRD_NRD_1	637	639	PF00675	0.558
CLV_NRD_NRD_1	659	661	PF00675	0.643
CLV_PCSK_FUR_1	29	33	PF00082	0.604
CLV_PCSK_FUR_1	54	58	PF00082	0.673
CLV_PCSK_FUR_1	588	592	PF00082	0.432
CLV_PCSK_KEX2_1	108	110	PF00082	0.722
CLV_PCSK_KEX2_1	255	257	PF00082	0.769
CLV_PCSK_KEX2_1	297	299	PF00082	0.648
CLV_PCSK_KEX2_1	308	310	PF00082	0.669
CLV_PCSK_KEX2_1	31	33	PF00082	0.577
CLV_PCSK_KEX2_1	346	348	PF00082	0.257
CLV_PCSK_KEX2_1	50	52	PF00082	0.696
CLV_PCSK_KEX2_1	56	58	PF00082	0.595
CLV_PCSK_KEX2_1	582	584	PF00082	0.408
CLV_PCSK_KEX2_1	590	592	PF00082	0.427
CLV_PCSK_KEX2_1	637	639	PF00082	0.554
CLV_PCSK_KEX2_1	659	661	PF00082	0.643
CLV_PCSK_PC1ET2_1	50	52	PF00082	0.732
CLV_PCSK_PC7_1	251	257	PF00082	0.616
CLV_PCSK_SKI1_1	114	118	PF00082	0.539
CLV_PCSK_SKI1_1	202	206	PF00082	0.710
CLV_PCSK_SKI1_1	242	246	PF00082	0.777
CLV_PCSK_SKI1_1	261	265	PF00082	0.628
CLV_PCSK_SKI1_1	470	474	PF00082	0.516
CLV_PCSK_SKI1_1	554	558	PF00082	0.403
CLV_PCSK_SKI1_1	596	600	PF00082	0.454
CLV_PCSK_SKI1_1	615	619	PF00082	0.334
DEG_APCC_DBOX_1	152	160	PF00400	0.609
DEG_APCC_DBOX_1	462	470	PF00400	0.507
DOC_CKS1_1	207	212	PF01111	0.692
DOC_CKS1_1	45	50	PF01111	0.683
DOC_MAPK_DCC_7	386	396	PF00069	0.393
DOC_MAPK_gen_1	108	115	PF00069	0.739
DOC_MAPK_gen_1	505	514	PF00069	0.456
DOC_MAPK_gen_1	659	667	PF00069	0.540
DOC_MAPK_MEF2A_6	388	396	PF00069	0.389
DOC_MAPK_MEF2A_6	505	514	PF00069	0.405
DOC_MIT_MIM_1	150	160	PF04212	0.476
DOC_PP1_RVXF_1	326	332	PF00149	0.390
DOC_PP1_RVXF_1	384	390	PF00149	0.413
DOC_PP1_RVXF_1	535	541	PF00149	0.377
DOC_PP2B_LxvP_1	647	650	PF13499	0.628
DOC_PP4_FxxP_1	389	392	PF00568	0.398
DOC_PP4_FxxP_1	499	502	PF00568	0.464
DOC_USP7_MATH_1	116	120	PF00917	0.597
DOC_USP7_MATH_1	224	228	PF00917	0.672
DOC_USP7_MATH_1	288	292	PF00917	0.553
DOC_USP7_MATH_1	319	323	PF00917	0.677
DOC_USP7_MATH_1	430	434	PF00917	0.457
DOC_USP7_MATH_1	503	507	PF00917	0.480
DOC_USP7_MATH_1	549	553	PF00917	0.410
DOC_USP7_MATH_1	654	658	PF00917	0.694
DOC_USP7_MATH_1	84	88	PF00917	0.754
DOC_WW_Pin1_4	141	146	PF00397	0.532
DOC_WW_Pin1_4	206	211	PF00397	0.704
DOC_WW_Pin1_4	266	271	PF00397	0.686
DOC_WW_Pin1_4	44	49	PF00397	0.685
DOC_WW_Pin1_4	596	601	PF00397	0.459
DOC_WW_Pin1_4	93	98	PF00397	0.626
LIG_14-3-3_CanoR_1	114	123	PF00244	0.533
LIG_14-3-3_CanoR_1	202	207	PF00244	0.635
LIG_14-3-3_CanoR_1	223	233	PF00244	0.569
LIG_14-3-3_CanoR_1	308	313	PF00244	0.638
LIG_14-3-3_CanoR_1	507	511	PF00244	0.416
LIG_14-3-3_CanoR_1	537	541	PF00244	0.363
LIG_14-3-3_CanoR_1	659	663	PF00244	0.640
LIG_Actin_WH2_2	144	162	PF00022	0.559
LIG_Actin_WH2_2	621	639	PF00022	0.589
LIG_BRCT_BRCA1_1	168	172	PF00533	0.684
LIG_deltaCOP1_diTrp_1	575	581	PF00928	0.407
LIG_EH_1	366	370	PF12763	0.457
LIG_FHA_1	147	153	PF00498	0.486
LIG_FHA_1	35	41	PF00498	0.609
LIG_FHA_1	462	468	PF00498	0.468
LIG_FHA_1	481	487	PF00498	0.423
LIG_FHA_2	226	232	PF00498	0.633
LIG_FHA_2	34	40	PF00498	0.598
LIG_FHA_2	597	603	PF00498	0.448
LIG_FHA_2	608	614	PF00498	0.425
LIG_Integrin_isoDGR_2	489	491	PF01839	0.599
LIG_Integrin_RGD_1	132	134	PF01839	0.739
LIG_LIR_Apic_2	497	502	PF02991	0.447
LIG_LIR_Gen_1	610	618	PF02991	0.437
LIG_LIR_Nem_3	539	543	PF02991	0.383
LIG_LIR_Nem_3	575	581	PF02991	0.413
LIG_LIR_Nem_3	610	614	PF02991	0.490
LIG_PCNA_PIPBox_1	1	10	PF02747	0.526
LIG_PTB_Apo_2	492	499	PF02174	0.463
LIG_PTB_Apo_2	605	612	PF02174	0.486
LIG_PTB_Phospho_1	605	611	PF10480	0.483
LIG_RPA_C_Fungi	342	354	PF08784	0.435
LIG_SH2_CRK	397	401	PF00017	0.301
LIG_SH2_SRC	611	614	PF00017	0.501
LIG_SH2_STAP1	508	512	PF00017	0.488
LIG_SH2_STAP1	562	566	PF00017	0.526
LIG_SH2_STAT5	465	468	PF00017	0.294
LIG_SH2_STAT5	580	583	PF00017	0.520
LIG_SH2_STAT5	609	612	PF00017	0.585
LIG_SH2_STAT5	7	10	PF00017	0.662
LIG_SH3_3	553	559	PF00018	0.397
LIG_SH3_3	566	572	PF00018	0.393
LIG_SUMO_SIM_par_1	440	445	PF11976	0.301
LIG_TRAF2_1	181	184	PF00917	0.567
LIG_TRAF2_1	24	27	PF00917	0.591
LIG_TRAF2_1	610	613	PF00917	0.467
LIG_TRAF2_2	145	150	PF00917	0.515
LIG_UBA3_1	156	163	PF00899	0.472
LIG_WRC_WIRS_1	61	66	PF05994	0.690
LIG_WW_1	559	562	PF00397	0.415
LIG_WW_3	390	394	PF00397	0.397
MOD_CDC14_SPxK_1	269	272	PF00782	0.504
MOD_CDK_SPK_2	44	49	PF00069	0.518
MOD_CDK_SPK_2	596	601	PF00069	0.459
MOD_CDK_SPxK_1	266	272	PF00069	0.511
MOD_CDK_SPxK_1	44	50	PF00069	0.682
MOD_CDK_SPxxK_3	44	51	PF00069	0.684
MOD_CK1_1	506	512	PF00069	0.402
MOD_CK2_1	33	39	PF00069	0.715
MOD_CK2_1	342	348	PF00069	0.301
MOD_CK2_1	549	555	PF00069	0.416
MOD_CK2_1	596	602	PF00069	0.445
MOD_CK2_1	607	613	PF00069	0.435
MOD_Cter_Amidation	306	309	PF01082	0.594
MOD_GlcNHglycan	18	21	PF01048	0.675
MOD_GlcNHglycan	280	283	PF01048	0.605
MOD_GlcNHglycan	321	324	PF01048	0.636
MOD_GlcNHglycan	354	357	PF01048	0.312
MOD_GlcNHglycan	58	61	PF01048	0.655
MOD_GlcNHglycan	81	84	PF01048	0.646
MOD_GlcNHglycan	86	89	PF01048	0.660
MOD_GSK3_1	18	25	PF00069	0.527
MOD_GSK3_1	202	209	PF00069	0.703
MOD_GSK3_1	308	315	PF00069	0.691
MOD_GSK3_1	403	410	PF00069	0.272
MOD_GSK3_1	56	63	PF00069	0.668
MOD_GSK3_1	654	661	PF00069	0.697
MOD_LATS_1	14	20	PF00433	0.462
MOD_N-GLC_1	361	366	PF02516	0.362
MOD_N-GLC_1	607	612	PF02516	0.493
MOD_NEK2_1	100	105	PF00069	0.600
MOD_NEK2_1	334	339	PF00069	0.440
MOD_NEK2_1	543	548	PF00069	0.413
MOD_NEK2_2	288	293	PF00069	0.613
MOD_NEK2_2	549	554	PF00069	0.414
MOD_NEK2_2	60	65	PF00069	0.657
MOD_NEK2_2	654	659	PF00069	0.697
MOD_PIKK_1	560	566	PF00454	0.534
MOD_PK_1	308	314	PF00069	0.628
MOD_PKA_1	308	314	PF00069	0.710
MOD_PKA_1	56	62	PF00069	0.682
MOD_PKA_2	308	314	PF00069	0.609
MOD_PKA_2	506	512	PF00069	0.400
MOD_PKA_2	536	542	PF00069	0.368
MOD_PKA_2	56	62	PF00069	0.716
MOD_PKA_2	584	590	PF00069	0.408
MOD_PKA_2	658	664	PF00069	0.717
MOD_PKB_1	54	62	PF00069	0.678
MOD_Plk_1	124	130	PF00069	0.699
MOD_Plk_1	283	289	PF00069	0.762
MOD_Plk_1	448	454	PF00069	0.319
MOD_Plk_1	554	560	PF00069	0.405
MOD_Plk_1	607	613	PF00069	0.486
MOD_Plk_1	688	694	PF00069	0.578
MOD_Plk_2-3	607	613	PF00069	0.451
MOD_Plk_4	168	174	PF00069	0.559
MOD_Plk_4	308	314	PF00069	0.706
MOD_Plk_4	456	462	PF00069	0.326
MOD_Plk_4	60	66	PF00069	0.579
MOD_ProDKin_1	141	147	PF00069	0.528
MOD_ProDKin_1	206	212	PF00069	0.703
MOD_ProDKin_1	266	272	PF00069	0.686
MOD_ProDKin_1	44	50	PF00069	0.682
MOD_ProDKin_1	596	602	PF00069	0.452
MOD_ProDKin_1	93	99	PF00069	0.627
MOD_SUMO_for_1	234	237	PF00179	0.663
MOD_SUMO_rev_2	154	161	PF00179	0.437
MOD_SUMO_rev_2	236	244	PF00179	0.635
MOD_SUMO_rev_2	607	617	PF00179	0.439
TRG_DiLeu_BaEn_1	241	246	PF01217	0.678
TRG_DiLeu_BaEn_2	326	332	PF01217	0.524
TRG_DiLeu_BaEn_2	448	454	PF01217	0.361
TRG_DiLeu_BaEn_2	687	693	PF01217	0.487
TRG_DiLeu_BaEn_4	183	189	PF01217	0.585
TRG_ENDOCYTIC_2	138	141	PF00928	0.713
TRG_ENDOCYTIC_2	611	614	PF00928	0.575
TRG_ER_diArg_1	177	180	PF00400	0.515
TRG_ER_diArg_1	29	32	PF00400	0.632
TRG_ER_diArg_1	296	298	PF00400	0.654
TRG_ER_diArg_1	386	389	PF00400	0.400
TRG_ER_diArg_1	467	470	PF00400	0.317
TRG_ER_diArg_1	48	51	PF00400	0.721
TRG_ER_diArg_1	581	583	PF00400	0.413
TRG_ER_diArg_1	588	591	PF00400	0.433
TRG_ER_diArg_1	636	638	PF00400	0.602
TRG_Pf-PMV_PEXEL_1	261	265	PF00026	0.716
TRG_Pf-PMV_PEXEL_1	421	426	PF00026	0.317
TRG_Pf-PMV_PEXEL_1	601	605	PF00026	0.400
TRG_Pf-PMV_PEXEL_1	640	644	PF00026	0.642

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I5W9	Leptomonas seymouri	69%	85%
A0A0S4IST3	Bodo saltans	41%	100%
A0A1X0P7U6	Trypanosomatidae	54%	100%
A0A3R7R867	Trypanosoma rangeli	54%	100%
A0A3S7XAP2	Leishmania donovani	93%	79%
A4HP30	Leishmania braziliensis	84%	100%
A4IDD7	Leishmania infantum	93%	79%
D0A313	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
E9AST8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
V5BPI0	Trypanosoma cruzi	56%	100%