Putative ER--golgi transport protein erv25

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

transmembrane emp24 domain-containing protein

Species:

Leishmania major

UniProt:

Q4Q1K6_LEIMA

TriTrypDb:

LmjF.36.2150 , LMJLV39_360029000 , LMJSD75_360029200

Length:

268

Annotations

LeishMANIAdb annotations

Conserved eukaryotic protein. Probable receptor for ER-Golgi trafficking of GPI-anchored proteins. Related to metazoan Tmed10.. Localization: ER (by homology) / Golgi (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 25

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 10, no: 0
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	2
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	2
GO:0005794	Golgi apparatus	5	2
GO:0016020	membrane	2	10
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	2
GO:0030135	coated vesicle	7	2
GO:0031410	cytoplasmic vesicle	6	2
GO:0031982	vesicle	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097708	intracellular vesicle	5	2
GO:0110165	cellular anatomical entity	1	10

Expansion

Sequence features

Q4Q1K6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q1K6

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0006886	intracellular protein transport	4	2
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	2
GO:0006996	organelle organization	4	2
GO:0007030	Golgi organization	5	2
GO:0008104	protein localization	4	2
GO:0009987	cellular process	1	2
GO:0015031	protein transport	4	2
GO:0016043	cellular component organization	3	2
GO:0016192	vesicle-mediated transport	4	2
GO:0033036	macromolecule localization	2	2
GO:0045184	establishment of protein localization	3	2
GO:0046907	intracellular transport	3	2
GO:0048193	Golgi vesicle transport	5	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0070727	cellular macromolecule localization	3	2
GO:0071702	organic substance transport	4	2
GO:0071705	nitrogen compound transport	4	2
GO:0071840	cellular component organization or biogenesis	2	2

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	87	91	PF00656	0.401
CLV_NRD_NRD_1	166	168	PF00675	0.571
CLV_NRD_NRD_1	262	264	PF00675	0.456
CLV_PCSK_KEX2_1	165	167	PF00082	0.504
CLV_PCSK_KEX2_1	205	207	PF00082	0.489
CLV_PCSK_PC1ET2_1	165	167	PF00082	0.504
CLV_PCSK_PC1ET2_1	205	207	PF00082	0.541
CLV_PCSK_SKI1_1	137	141	PF00082	0.558
CLV_PCSK_SKI1_1	185	189	PF00082	0.504
CLV_PCSK_SKI1_1	20	24	PF00082	0.686
CLV_PCSK_SKI1_1	205	209	PF00082	0.357
CLV_PCSK_SKI1_1	256	260	PF00082	0.321
DEG_APCC_DBOX_1	190	198	PF00400	0.370
DEG_MDM2_SWIB_1	233	241	PF02201	0.370
DOC_CDC14_PxL_1	15	23	PF14671	0.468
DOC_CYCLIN_RxL_1	199	209	PF00134	0.227
DOC_MAPK_gen_1	137	145	PF00069	0.391
DOC_MAPK_gen_1	165	174	PF00069	0.315
DOC_MAPK_MEF2A_6	137	145	PF00069	0.391
DOC_PP1_RVXF_1	47	54	PF00149	0.370
DOC_PP1_RVXF_1	8	14	PF00149	0.653
DOC_USP7_MATH_1	34	38	PF00917	0.583
DOC_WW_Pin1_4	74	79	PF00397	0.393
LIG_14-3-3_CanoR_1	10	16	PF00244	0.580
LIG_14-3-3_CanoR_1	224	233	PF00244	0.247
LIG_Actin_WH2_2	243	261	PF00022	0.363
LIG_BRCT_BRCA1_1	127	131	PF00533	0.393
LIG_CORNRBOX	243	251	PF00104	0.488
LIG_FHA_1	202	208	PF00498	0.249
LIG_FHA_1	60	66	PF00498	0.314
LIG_FHA_1	90	96	PF00498	0.343
LIG_FHA_2	174	180	PF00498	0.290
LIG_IRF3_LxIS_1	211	218	PF10401	0.227
LIG_LIR_Gen_1	230	241	PF02991	0.312
LIG_LIR_Gen_1	42	51	PF02991	0.387
LIG_LIR_Nem_3	230	236	PF02991	0.321
LIG_LIR_Nem_3	42	46	PF02991	0.355
LIG_NRBOX	20	26	PF00104	0.515
LIG_Pex14_2	233	237	PF04695	0.356
LIG_SH2_SRC	177	180	PF00017	0.399
LIG_SH2_STAP1	97	101	PF00017	0.393
LIG_SH2_STAT3	175	178	PF00017	0.393
LIG_SH2_STAT5	175	178	PF00017	0.358
LIG_SH2_STAT5	193	196	PF00017	0.183
LIG_TRAF2_1	176	179	PF00917	0.393
LIG_TYR_ITIM	41	46	PF00017	0.338
MOD_CK1_1	229	235	PF00069	0.313
MOD_CK1_1	63	69	PF00069	0.393
MOD_CK1_1	74	80	PF00069	0.385
MOD_CK2_1	173	179	PF00069	0.370
MOD_GlcNHglycan	117	120	PF01048	0.546
MOD_GlcNHglycan	200	204	PF01048	0.586
MOD_GlcNHglycan	247	250	PF01048	0.455
MOD_GlcNHglycan	36	39	PF01048	0.616
MOD_GlcNHglycan	73	76	PF01048	0.544
MOD_GlcNHglycan	86	89	PF01048	0.487
MOD_GSK3_1	225	232	PF00069	0.275
MOD_GSK3_1	59	66	PF00069	0.283
MOD_NEK2_1	1	6	PF00069	0.626
MOD_NEK2_1	125	130	PF00069	0.393
MOD_NEK2_1	201	206	PF00069	0.385
MOD_NEK2_1	215	220	PF00069	0.361
MOD_NEK2_1	257	262	PF00069	0.520
MOD_NEK2_1	32	37	PF00069	0.553
MOD_NEK2_1	84	89	PF00069	0.364
MOD_PIKK_1	60	66	PF00454	0.367
MOD_PIKK_1	77	83	PF00454	0.193
MOD_PKA_2	225	231	PF00069	0.300
MOD_PKA_2	84	90	PF00069	0.364
MOD_PKB_1	224	232	PF00069	0.321
MOD_Plk_1	104	110	PF00069	0.370
MOD_Plk_1	229	235	PF00069	0.335
MOD_Plk_2-3	173	179	PF00069	0.290
MOD_ProDKin_1	74	80	PF00069	0.393
MOD_SUMO_for_1	149	152	PF00179	0.380
MOD_SUMO_rev_2	129	139	PF00179	0.379
MOD_SUMO_rev_2	179	187	PF00179	0.342
TRG_DiLeu_BaLyEn_6	17	22	PF01217	0.523
TRG_DiLeu_BaLyEn_6	203	208	PF01217	0.338
TRG_ENDOCYTIC_2	43	46	PF00928	0.364
TRG_ER_diLys_1	264	268	PF00400	0.624
TRG_NLS_MonoCore_2	262	267	PF00514	0.669
TRG_NLS_MonoExtC_3	164	170	PF00514	0.321
TRG_NLS_MonoExtN_4	263	268	PF00514	0.684
TRG_Pf-PMV_PEXEL_1	206	210	PF00026	0.538

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7A9	Leptomonas seymouri	55%	100%
A0A1X0P7Z0	Trypanosomatidae	33%	100%
A0A3S7XAW3	Leishmania donovani	93%	100%
A0A422N537	Trypanosoma rangeli	27%	100%
A4HP84	Leishmania braziliensis	79%	100%
A4IDI9	Leishmania infantum	93%	100%
D0A380	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
E9ASZ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
P0CN72	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	26%	100%
P0CN73	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	26%	100%
V5BBT5	Trypanosoma cruzi	34%	100%