Putative serine/threonine protein kinase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

NEK family Serine/threonine-protein kinase, putative

Species:

Leishmania major

UniProt:

Q4Q1J2_LEIMA

TriTrypDb:

LmjF.36.2290 , LMJLV39_360030400 , LMJSD75_360030600

Length:

476

Annotations

LeishMANIAdb annotations

A large and apprently artificial collection of diverse kinetoplastid protein kinases. None of them appear to be TM.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 16

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 26
NetGPI	no	yes: 0, no: 26

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005952	cAMP-dependent protein kinase complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0051286	cell tip	3	1
GO:0060187	cell pole	2	1
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	1
GO:0110165	cellular anatomical entity	1	1
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1902554	serine/threonine protein kinase complex	6	1
GO:1902911	protein kinase complex	5	1
GO:1990234	transferase complex	3	1

Expansion

Sequence features

Q4Q1J2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q1J2

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	27
GO:0006793	phosphorus metabolic process	3	27
GO:0006796	phosphate-containing compound metabolic process	4	27
GO:0006807	nitrogen compound metabolic process	2	27
GO:0008152	metabolic process	1	27
GO:0009987	cellular process	1	27
GO:0016310	phosphorylation	5	27
GO:0019538	protein metabolic process	3	27
GO:0036211	protein modification process	4	27
GO:0043170	macromolecule metabolic process	3	27
GO:0043412	macromolecule modification	4	27
GO:0044237	cellular metabolic process	2	27
GO:0044238	primary metabolic process	2	27
GO:0071704	organic substance metabolic process	2	27
GO:1901564	organonitrogen compound metabolic process	3	27
GO:0007165	signal transduction	2	1
GO:0018105	peptidyl-serine phosphorylation	6	1
GO:0018193	peptidyl-amino acid modification	5	1
GO:0018209	peptidyl-serine modification	6	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0065007	biological regulation	1	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	27
GO:0003824	catalytic activity	1	27
GO:0004672	protein kinase activity	3	27
GO:0004674	protein serine/threonine kinase activity	4	26
GO:0005488	binding	1	27
GO:0005524	ATP binding	5	27
GO:0016301	kinase activity	4	27
GO:0016740	transferase activity	2	27
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	27
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	27
GO:0017076	purine nucleotide binding	4	27
GO:0030554	adenyl nucleotide binding	5	27
GO:0032553	ribonucleotide binding	3	27
GO:0032555	purine ribonucleotide binding	4	27
GO:0032559	adenyl ribonucleotide binding	5	27
GO:0035639	purine ribonucleoside triphosphate binding	4	27
GO:0036094	small molecule binding	2	27
GO:0043167	ion binding	2	27
GO:0043168	anion binding	3	27
GO:0097159	organic cyclic compound binding	2	27
GO:0097367	carbohydrate derivative binding	2	27
GO:0140096	catalytic activity, acting on a protein	2	27
GO:1901265	nucleoside phosphate binding	3	27
GO:1901363	heterocyclic compound binding	2	27
GO:0004690	cyclic nucleotide-dependent protein kinase activity	5	1
GO:0004691	cAMP-dependent protein kinase activity	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	58	62	PF00656	0.590
CLV_NRD_NRD_1	124	126	PF00675	0.411
CLV_NRD_NRD_1	210	212	PF00675	0.408
CLV_NRD_NRD_1	216	218	PF00675	0.374
CLV_NRD_NRD_1	392	394	PF00675	0.426
CLV_NRD_NRD_1	418	420	PF00675	0.405
CLV_NRD_NRD_1	473	475	PF00675	0.449
CLV_PCSK_FUR_1	122	126	PF00082	0.405
CLV_PCSK_FUR_1	214	218	PF00082	0.170
CLV_PCSK_FUR_1	471	475	PF00082	0.602
CLV_PCSK_KEX2_1	124	126	PF00082	0.411
CLV_PCSK_KEX2_1	210	212	PF00082	0.368
CLV_PCSK_KEX2_1	214	216	PF00082	0.329
CLV_PCSK_KEX2_1	392	394	PF00082	0.433
CLV_PCSK_KEX2_1	418	420	PF00082	0.420
CLV_PCSK_KEX2_1	473	475	PF00082	0.598
CLV_PCSK_PC7_1	120	126	PF00082	0.411
CLV_PCSK_PC7_1	210	216	PF00082	0.301
CLV_PCSK_SKI1_1	216	220	PF00082	0.313
CLV_PCSK_SKI1_1	375	379	PF00082	0.274
DEG_APCC_DBOX_1	100	108	PF00400	0.268
DEG_APCC_DBOX_1	384	392	PF00400	0.478
DEG_Nend_UBRbox_1	1	4	PF02207	0.448
DEG_SCF_FBW7_2	51	58	PF00400	0.511
DOC_CKS1_1	52	57	PF01111	0.514
DOC_CYCLIN_yCln2_LP_2	324	330	PF00134	0.263
DOC_MAPK_MEF2A_6	63	70	PF00069	0.600
DOC_PP4_FxxP_1	7	10	PF00568	0.378
DOC_USP7_MATH_1	34	38	PF00917	0.625
DOC_USP7_MATH_1	405	409	PF00917	0.265
DOC_USP7_MATH_1	428	432	PF00917	0.682
DOC_WW_Pin1_4	144	149	PF00397	0.473
DOC_WW_Pin1_4	177	182	PF00397	0.200
DOC_WW_Pin1_4	218	223	PF00397	0.337
DOC_WW_Pin1_4	288	293	PF00397	0.315
DOC_WW_Pin1_4	45	50	PF00397	0.680
DOC_WW_Pin1_4	464	469	PF00397	0.638
DOC_WW_Pin1_4	51	56	PF00397	0.667
LIG_14-3-3_CanoR_1	215	223	PF00244	0.237
LIG_14-3-3_CanoR_1	283	292	PF00244	0.234
LIG_14-3-3_CanoR_1	304	308	PF00244	0.270
LIG_14-3-3_CanoR_1	392	401	PF00244	0.459
LIG_APCC_ABBA_1	53	58	PF00400	0.515
LIG_Clathr_ClatBox_1	198	202	PF01394	0.361
LIG_Clathr_ClatBox_1	363	367	PF01394	0.344
LIG_eIF4E_1	90	96	PF01652	0.400
LIG_FHA_1	224	230	PF00498	0.342
LIG_FHA_1	260	266	PF00498	0.315
LIG_FHA_1	275	281	PF00498	0.241
LIG_FHA_1	372	378	PF00498	0.247
LIG_FHA_2	222	228	PF00498	0.344
LIG_FHA_2	330	336	PF00498	0.329
LIG_Integrin_RGD_1	301	303	PF01839	0.200
LIG_IRF3_LxIS_1	66	71	PF10401	0.421
LIG_LIR_Apic_2	291	297	PF02991	0.320
LIG_LIR_Apic_2	5	10	PF02991	0.374
LIG_LIR_Gen_1	227	238	PF02991	0.323
LIG_LIR_Gen_1	342	352	PF02991	0.198
LIG_LIR_Gen_1	358	368	PF02991	0.240
LIG_LIR_Gen_1	408	417	PF02991	0.424
LIG_LIR_Nem_3	110	114	PF02991	0.345
LIG_LIR_Nem_3	227	233	PF02991	0.323
LIG_LIR_Nem_3	342	348	PF02991	0.367
LIG_LIR_Nem_3	358	364	PF02991	0.217
LIG_LIR_Nem_3	408	413	PF02991	0.361
LIG_NRBOX	317	323	PF00104	0.279
LIG_Pex14_2	432	436	PF04695	0.475
LIG_SH2_CRK	111	115	PF00017	0.403
LIG_SH2_CRK	345	349	PF00017	0.200
LIG_SH2_CRK	361	365	PF00017	0.291
LIG_SH2_CRK	90	94	PF00017	0.326
LIG_SH2_GRB2like	352	355	PF00017	0.200
LIG_SH2_NCK_1	111	115	PF00017	0.362
LIG_SH2_SRC	149	152	PF00017	0.440
LIG_SH2_STAP1	319	323	PF00017	0.354
LIG_SH2_STAP1	352	356	PF00017	0.309
LIG_SH2_STAT5	203	206	PF00017	0.465
LIG_SH2_STAT5	276	279	PF00017	0.192
LIG_SH2_STAT5	294	297	PF00017	0.321
LIG_SH2_STAT5	383	386	PF00017	0.427
LIG_SH2_STAT5	94	97	PF00017	0.316
LIG_SH3_3	145	151	PF00018	0.461
LIG_SH3_3	157	163	PF00018	0.451
LIG_SH3_3	457	463	PF00018	0.526
LIG_SH3_3	46	52	PF00018	0.609
LIG_SUMO_SIM_anti_2	196	202	PF11976	0.286
LIG_SUMO_SIM_par_1	320	325	PF11976	0.381
LIG_SUMO_SIM_par_1	362	367	PF11976	0.350
LIG_TRAF2_1	151	154	PF00917	0.441
LIG_TRFH_1	293	297	PF08558	0.283
LIG_TYR_ITIM	317	322	PF00017	0.412
LIG_TYR_ITIM	359	364	PF00017	0.291
MOD_CDK_SPK_2	45	50	PF00069	0.505
MOD_CDK_SPxxK_3	464	471	PF00069	0.490
MOD_CK1_1	134	140	PF00069	0.478
MOD_CK1_1	306	312	PF00069	0.301
MOD_CK1_1	395	401	PF00069	0.480
MOD_CK1_1	5	11	PF00069	0.397
MOD_CK1_1	69	75	PF00069	0.479
MOD_CK2_1	221	227	PF00069	0.344
MOD_CK2_1	329	335	PF00069	0.373
MOD_GlcNHglycan	413	416	PF01048	0.286
MOD_GlcNHglycan	422	425	PF01048	0.566
MOD_GlcNHglycan	448	451	PF01048	0.752
MOD_GSK3_1	274	281	PF00069	0.343
MOD_GSK3_1	284	291	PF00069	0.316
MOD_GSK3_1	395	402	PF00069	0.433
MOD_GSK3_1	405	412	PF00069	0.392
MOD_GSK3_1	436	443	PF00069	0.675
MOD_GSK3_1	5	12	PF00069	0.412
MOD_GSK3_1	66	73	PF00069	0.601
MOD_N-GLC_1	399	404	PF02516	0.338
MOD_NEK2_1	258	263	PF00069	0.293
MOD_NEK2_1	322	327	PF00069	0.470
MOD_NEK2_1	409	414	PF00069	0.368
MOD_NEK2_1	68	73	PF00069	0.630
MOD_NEK2_2	405	410	PF00069	0.433
MOD_NEK2_2	436	441	PF00069	0.647
MOD_PIKK_1	278	284	PF00454	0.288
MOD_PK_1	2	8	PF00069	0.425
MOD_PKA_1	216	222	PF00069	0.170
MOD_PKA_1	274	280	PF00069	0.218
MOD_PKA_1	392	398	PF00069	0.305
MOD_PKA_2	216	222	PF00069	0.176
MOD_PKA_2	303	309	PF00069	0.258
MOD_PKA_2	370	376	PF00069	0.283
MOD_PKA_2	392	398	PF00069	0.452
MOD_PKB_1	214	222	PF00069	0.170
MOD_Plk_1	395	401	PF00069	0.318
MOD_Plk_1	66	72	PF00069	0.577
MOD_Plk_4	2	8	PF00069	0.425
MOD_Plk_4	405	411	PF00069	0.322
MOD_Plk_4	9	15	PF00069	0.388
MOD_ProDKin_1	144	150	PF00069	0.471
MOD_ProDKin_1	177	183	PF00069	0.200
MOD_ProDKin_1	218	224	PF00069	0.337
MOD_ProDKin_1	288	294	PF00069	0.315
MOD_ProDKin_1	45	51	PF00069	0.677
MOD_ProDKin_1	464	470	PF00069	0.636
MOD_SUMO_for_1	252	255	PF00179	0.311
MOD_SUMO_for_1	307	310	PF00179	0.200
MOD_SUMO_rev_2	398	408	PF00179	0.320
TRG_DiLeu_BaEn_1	196	201	PF01217	0.242
TRG_ENDOCYTIC_2	111	114	PF00928	0.411
TRG_ENDOCYTIC_2	319	322	PF00928	0.365
TRG_ENDOCYTIC_2	345	348	PF00928	0.341
TRG_ENDOCYTIC_2	361	364	PF00928	0.291
TRG_ENDOCYTIC_2	90	93	PF00928	0.324
TRG_ER_diArg_1	122	125	PF00400	0.406
TRG_ER_diArg_1	209	211	PF00400	0.381
TRG_ER_diArg_1	213	216	PF00400	0.344
TRG_ER_diArg_1	391	393	PF00400	0.406
TRG_ER_diArg_1	417	419	PF00400	0.381
TRG_ER_diArg_1	471	474	PF00400	0.456
TRG_Pf-PMV_PEXEL_1	375	379	PF00026	0.281
TRG_Pf-PMV_PEXEL_1	392	396	PF00026	0.545

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4J6A0	Bodo saltans	25%	67%
A0A0S4JI45	Bodo saltans	24%	73%
A0A0S4JK24	Bodo saltans	30%	100%
A0A1X0NIX2	Trypanosomatidae	36%	100%
A0A1X0NUB2	Trypanosomatidae	29%	82%
A0A1X0NWR7	Trypanosomatidae	26%	93%
A0A3Q8IAQ1	Leishmania donovani	40%	100%
A0A3Q8IB30	Leishmania donovani	33%	100%
A0A3Q8IC87	Leishmania donovani	29%	100%
A0A3Q8IFK8	Leishmania donovani	39%	100%
A0A3S7X9R4	Leishmania donovani	27%	100%
A0A3S7X9S2	Leishmania donovani	27%	100%
A0A3S7XAX0	Leishmania donovani	93%	100%
A0A422P4V9	Trypanosoma rangeli	23%	100%
A4H7V0	Leishmania braziliensis	25%	67%
A4H9L8	Leishmania braziliensis	32%	100%
A4HCD7	Leishmania braziliensis	28%	100%
A4HCE6	Leishmania braziliensis	37%	100%
A4HJT5	Leishmania braziliensis	39%	100%
A4HXY2	Leishmania infantum	33%	100%
A4HZV1	Leishmania infantum	29%	100%
A4HZW8	Leishmania infantum	40%	100%
A4I7A1	Leishmania infantum	39%	100%
A4IBT4	Leishmania infantum	27%	100%
A4IBT9	Leishmania infantum	27%	100%
A4IDK3	Leishmania infantum	94%	100%
A5GFW1	Sus scrofa	25%	100%
C9ZWK2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
D0A7M4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	69%
D0AA64	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	83%
E9APX7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	67%
E9ARP5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9AT06	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	99%
E9AVS7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
E9B6S9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
O59790	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	100%
P51817	Homo sapiens	27%	100%
P90980	Caenorhabditis elegans	27%	70%
P92958	Arabidopsis thaliana	28%	93%
Q27687	Leishmania major	33%	100%
Q2TA06	Bos taurus	27%	100%
Q38997	Arabidopsis thaliana	28%	93%
Q4Q598	Leishmania major	31%	73%
Q4Q5W2	Leishmania major	39%	100%
Q4QBQ2	Leishmania major	40%	100%
Q4QBR6	Leishmania major	28%	100%
Q4QFK4	Leishmania major	25%	76%
Q641K5	Mus musculus	26%	72%
Q6FV07	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	26%	100%
Q7XIW5	Oryza sativa subsp. japonica	28%	100%
Q869W6	Dictyostelium discoideum	28%	100%
Q8C0Q4	Mus musculus	28%	76%
Q8NG66	Homo sapiens	28%	74%
Q8WNU8	Macaca fascicularis	27%	75%
Q91819	Xenopus laevis	25%	100%
Q91820	Xenopus laevis	25%	100%
Q9SLI2	Arabidopsis thaliana	27%	78%
Q9SN43	Arabidopsis thaliana	28%	97%
Q9UBS0	Homo sapiens	27%	99%
V5DC40	Trypanosoma cruzi	24%	87%
V5DFW9	Trypanosoma cruzi	28%	82%