Putative DEAD/DEAH box helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ATP-dependent DEAD/H RNA helicase, putative

Species:

Leishmania major

UniProt:

Q4Q1G8_LEIMA

TriTrypDb:

LmjF.36.2530 , LMJLV39_360033300 , LMJSD75_360033300

Length:

702

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 10

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 25
NetGPI	no	yes: 0, no: 25

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	4
GO:0005739	mitochondrion	5	3
GO:0043226	organelle	2	4
GO:0043227	membrane-bounded organelle	3	4
GO:0043229	intracellular organelle	3	4
GO:0043231	intracellular membrane-bounded organelle	4	4
GO:0110165	cellular anatomical entity	1	4
GO:0005634	nucleus	5	2
GO:0010494	cytoplasmic stress granule	5	1
GO:0034399	nuclear periphery	2	1
GO:0035770	ribonucleoprotein granule	3	1
GO:0036464	cytoplasmic ribonucleoprotein granule	4	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0097165	nuclear stress granule	4	1
GO:0099080	supramolecular complex	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1

Expansion

Sequence features

Q4Q1G8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q1G8

Function

Biological processes
Term	Name	Level	Count
GO:0000027	ribosomal large subunit assembly	7	2
GO:0009987	cellular process	1	3
GO:0016043	cellular component organization	3	2
GO:0022607	cellular component assembly	4	2
GO:0022618	ribonucleoprotein complex assembly	6	2
GO:0043933	protein-containing complex organization	4	2
GO:0065003	protein-containing complex assembly	5	2
GO:0071826	ribonucleoprotein complex subunit organization	5	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:1902775	mitochondrial large ribosomal subunit assembly	8	2
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0016070	RNA metabolic process	5	1
GO:0016071	mRNA metabolic process	6	1
GO:0016556	mRNA modification	6	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	26
GO:0003676	nucleic acid binding	3	26
GO:0003824	catalytic activity	1	26
GO:0004386	helicase activity	2	26
GO:0005488	binding	1	26
GO:0005524	ATP binding	5	26
GO:0017076	purine nucleotide binding	4	26
GO:0030554	adenyl nucleotide binding	5	26
GO:0032553	ribonucleotide binding	3	26
GO:0032555	purine ribonucleotide binding	4	26
GO:0032559	adenyl ribonucleotide binding	5	26
GO:0035639	purine ribonucleoside triphosphate binding	4	26
GO:0036094	small molecule binding	2	26
GO:0043167	ion binding	2	26
GO:0043168	anion binding	3	26
GO:0097159	organic cyclic compound binding	2	26
GO:0097367	carbohydrate derivative binding	2	26
GO:0140640	catalytic activity, acting on a nucleic acid	2	26
GO:0140657	ATP-dependent activity	1	26
GO:1901265	nucleoside phosphate binding	3	26
GO:1901363	heterocyclic compound binding	2	26
GO:0003723	RNA binding	4	3
GO:0016787	hydrolase activity	2	11
GO:0003724	RNA helicase activity	3	6
GO:0003743	translation initiation factor activity	4	5
GO:0008135	translation factor activity, RNA binding	3	5
GO:0008186	ATP-dependent activity, acting on RNA	2	6
GO:0045182	translation regulator activity	1	5
GO:0090079	translation regulator activity, nucleic acid binding	2	5
GO:0140098	catalytic activity, acting on RNA	3	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	73	77	PF00656	0.745
CLV_NRD_NRD_1	143	145	PF00675	0.464
CLV_NRD_NRD_1	216	218	PF00675	0.321
CLV_NRD_NRD_1	270	272	PF00675	0.280
CLV_NRD_NRD_1	325	327	PF00675	0.291
CLV_NRD_NRD_1	358	360	PF00675	0.320
CLV_NRD_NRD_1	668	670	PF00675	0.595
CLV_NRD_NRD_1	685	687	PF00675	0.691
CLV_NRD_NRD_1	85	87	PF00675	0.696
CLV_PCSK_FUR_1	271	275	PF00082	0.187
CLV_PCSK_KEX2_1	145	147	PF00082	0.446
CLV_PCSK_KEX2_1	216	218	PF00082	0.280
CLV_PCSK_KEX2_1	270	272	PF00082	0.280
CLV_PCSK_KEX2_1	273	275	PF00082	0.280
CLV_PCSK_KEX2_1	358	360	PF00082	0.220
CLV_PCSK_KEX2_1	685	687	PF00082	0.691
CLV_PCSK_KEX2_1	85	87	PF00082	0.591
CLV_PCSK_PC1ET2_1	145	147	PF00082	0.369
CLV_PCSK_PC1ET2_1	273	275	PF00082	0.187
CLV_PCSK_PC7_1	266	272	PF00082	0.321
CLV_PCSK_SKI1_1	11	15	PF00082	0.524
CLV_PCSK_SKI1_1	148	152	PF00082	0.486
CLV_PCSK_SKI1_1	165	169	PF00082	0.184
CLV_PCSK_SKI1_1	270	274	PF00082	0.187
CLV_PCSK_SKI1_1	381	385	PF00082	0.262
CLV_PCSK_SKI1_1	396	400	PF00082	0.239
CLV_PCSK_SKI1_1	414	418	PF00082	0.550
CLV_PCSK_SKI1_1	421	425	PF00082	0.372
CLV_PCSK_SKI1_1	624	628	PF00082	0.376
DEG_APCC_DBOX_1	134	142	PF00400	0.349
DEG_APCC_DBOX_1	325	333	PF00400	0.478
DEG_APCC_DBOX_1	420	428	PF00400	0.394
DEG_APCC_DBOX_1	477	485	PF00400	0.534
DEG_Nend_Nbox_1	1	3	PF02207	0.486
DOC_CKS1_1	149	154	PF01111	0.193
DOC_CKS1_1	427	432	PF01111	0.453
DOC_CYCLIN_RxL_1	393	402	PF00134	0.430
DOC_CYCLIN_RxL_1	411	419	PF00134	0.394
DOC_CYCLIN_yCln2_LP_2	109	115	PF00134	0.510
DOC_CYCLIN_yCln2_LP_2	427	433	PF00134	0.577
DOC_CYCLIN_yCln2_LP_2	639	642	PF00134	0.398
DOC_CYCLIN_yCln2_LP_2	95	101	PF00134	0.644
DOC_MAPK_JIP1_4	393	399	PF00069	0.439
DOC_PP1_RVXF_1	494	500	PF00149	0.285
DOC_PP1_RVXF_1	509	516	PF00149	0.509
DOC_PP1_RVXF_1	571	578	PF00149	0.429
DOC_PP1_SILK_1	131	136	PF00149	0.358
DOC_PP2B_LxvP_1	109	112	PF13499	0.529
DOC_PP2B_LxvP_1	639	642	PF13499	0.398
DOC_PP2B_LxvP_1	95	98	PF13499	0.667
DOC_PP4_FxxP_1	251	254	PF00568	0.496
DOC_PP4_FxxP_1	617	620	PF00568	0.354
DOC_PP4_FxxP_1	661	664	PF00568	0.519
DOC_USP7_MATH_1	252	256	PF00917	0.533
DOC_USP7_MATH_1	264	268	PF00917	0.519
DOC_USP7_MATH_1	436	440	PF00917	0.657
DOC_USP7_MATH_1	45	49	PF00917	0.532
DOC_USP7_MATH_1	545	549	PF00917	0.442
DOC_USP7_MATH_1	648	652	PF00917	0.459
DOC_USP7_MATH_1	673	677	PF00917	0.694
DOC_WW_Pin1_4	127	132	PF00397	0.502
DOC_WW_Pin1_4	148	153	PF00397	0.416
DOC_WW_Pin1_4	339	344	PF00397	0.482
DOC_WW_Pin1_4	426	431	PF00397	0.473
DOC_WW_Pin1_4	446	451	PF00397	0.722
DOC_WW_Pin1_4	674	679	PF00397	0.680
LIG_14-3-3_CanoR_1	28	34	PF00244	0.542
LIG_14-3-3_CanoR_1	358	364	PF00244	0.493
LIG_14-3-3_CanoR_1	441	446	PF00244	0.788
LIG_14-3-3_CanoR_1	558	566	PF00244	0.520
LIG_Actin_WH2_2	179	194	PF00022	0.452
LIG_Actin_WH2_2	53	71	PF00022	0.500
LIG_AP2alpha_1	652	656	PF02296	0.490
LIG_APCC_ABBA_1	232	237	PF00400	0.521
LIG_BIR_III_4	107	111	PF00653	0.531
LIG_BIR_III_4	37	41	PF00653	0.566
LIG_BRCT_BRCA1_1	300	304	PF00533	0.412
LIG_BRCT_BRCA1_1	418	422	PF00533	0.378
LIG_EH1_1	310	318	PF00400	0.425
LIG_eIF4E_1	181	187	PF01652	0.521
LIG_FHA_1	411	417	PF00498	0.494
LIG_FHA_1	501	507	PF00498	0.456
LIG_FHA_1	561	567	PF00498	0.480
LIG_FHA_1	675	681	PF00498	0.682
LIG_FHA_2	427	433	PF00498	0.546
LIG_FHA_2	588	594	PF00498	0.482
LIG_LIR_Apic_2	249	254	PF02991	0.496
LIG_LIR_Apic_2	616	620	PF02991	0.345
LIG_LIR_Apic_2	659	664	PF02991	0.502
LIG_LIR_Gen_1	122	131	PF02991	0.529
LIG_LIR_Gen_1	419	430	PF02991	0.428
LIG_LIR_Gen_1	651	660	PF02991	0.563
LIG_LIR_Nem_3	122	126	PF02991	0.465
LIG_LIR_Nem_3	163	167	PF02991	0.525
LIG_LIR_Nem_3	22	27	PF02991	0.714
LIG_LIR_Nem_3	301	307	PF02991	0.555
LIG_LIR_Nem_3	419	425	PF02991	0.405
LIG_LIR_Nem_3	576	580	PF02991	0.432
LIG_LIR_Nem_3	651	655	PF02991	0.549
LIG_LIR_Nem_3	684	690	PF02991	0.583
LIG_NRBOX	185	191	PF00104	0.478
LIG_OCRL_FandH_1	303	315	PF00620	0.425
LIG_PCNA_PIPBox_1	692	701	PF02747	0.523
LIG_Pex14_2	652	656	PF04695	0.490
LIG_PTB_Apo_2	655	662	PF02174	0.529
LIG_SH2_CRK	101	105	PF00017	0.460
LIG_SH2_CRK	147	151	PF00017	0.543
LIG_SH2_CRK	183	187	PF00017	0.508
LIG_SH2_CRK	586	590	PF00017	0.505
LIG_SH2_NCK_1	586	590	PF00017	0.496
LIG_SH2_SRC	181	184	PF00017	0.547
LIG_SH2_STAP1	183	187	PF00017	0.521
LIG_SH2_STAT3	632	635	PF00017	0.443
LIG_SH2_STAT3	647	650	PF00017	0.347
LIG_SH2_STAT5	181	184	PF00017	0.463
LIG_SH2_STAT5	235	238	PF00017	0.439
LIG_SH2_STAT5	328	331	PF00017	0.512
LIG_SH2_STAT5	524	527	PF00017	0.490
LIG_SH2_STAT5	582	585	PF00017	0.472
LIG_SH2_STAT5	613	616	PF00017	0.312
LIG_SH2_STAT5	632	635	PF00017	0.545
LIG_SH3_2	664	669	PF14604	0.581
LIG_SH3_3	146	152	PF00018	0.310
LIG_SH3_3	279	285	PF00018	0.483
LIG_SH3_3	286	292	PF00018	0.503
LIG_SH3_3	3	9	PF00018	0.540
LIG_SH3_3	337	343	PF00018	0.554
LIG_SH3_3	388	394	PF00018	0.570
LIG_SH3_3	447	453	PF00018	0.769
LIG_SH3_3	61	67	PF00018	0.719
LIG_SH3_3	661	667	PF00018	0.537
LIG_SH3_3	86	92	PF00018	0.572
LIG_SUMO_SIM_par_1	102	107	PF11976	0.348
LIG_SUMO_SIM_par_1	124	130	PF11976	0.462
LIG_SUMO_SIM_par_1	158	163	PF11976	0.418
LIG_SUMO_SIM_par_1	423	429	PF11976	0.413
LIG_TRAF2_1	321	324	PF00917	0.440
LIG_TYR_ITIM	162	167	PF00017	0.526
LIG_TYR_ITIM	406	411	PF00017	0.399
LIG_UBA3_1	186	192	PF00899	0.532
LIG_UBA3_1	346	354	PF00899	0.439
LIG_UBA3_1	383	389	PF00899	0.421
LIG_WRC_WIRS_1	219	224	PF05994	0.425
LIG_WRC_WIRS_1	614	619	PF05994	0.400
LIG_WW_3	640	644	PF00397	0.342
MOD_CDC14_SPxK_1	449	452	PF00782	0.546
MOD_CDK_SPxK_1	446	452	PF00069	0.546
MOD_CK1_1	298	304	PF00069	0.433
MOD_CK1_1	32	38	PF00069	0.727
MOD_CK1_1	342	348	PF00069	0.427
MOD_CK1_1	443	449	PF00069	0.612
MOD_CK1_1	543	549	PF00069	0.448
MOD_CK2_1	252	258	PF00069	0.457
MOD_CK2_1	278	284	PF00069	0.461
MOD_CK2_1	317	323	PF00069	0.429
MOD_CK2_1	426	432	PF00069	0.587
MOD_CK2_1	545	551	PF00069	0.437
MOD_CK2_1	587	593	PF00069	0.450
MOD_CK2_1	77	83	PF00069	0.704
MOD_DYRK1A_RPxSP_1	148	152	PF00069	0.338
MOD_GlcNHglycan	21	24	PF01048	0.739
MOD_GlcNHglycan	254	257	PF01048	0.248
MOD_GlcNHglycan	300	303	PF01048	0.407
MOD_GlcNHglycan	31	34	PF01048	0.627
MOD_GlcNHglycan	320	323	PF01048	0.259
MOD_GlcNHglycan	438	441	PF01048	0.634
MOD_GlcNHglycan	46	50	PF01048	0.695
MOD_GlcNHglycan	466	469	PF01048	0.578
MOD_GlcNHglycan	547	550	PF01048	0.265
MOD_GlcNHglycan	76	80	PF01048	0.711
MOD_GSK3_1	129	136	PF00069	0.484
MOD_GSK3_1	15	22	PF00069	0.690
MOD_GSK3_1	254	261	PF00069	0.487
MOD_GSK3_1	28	35	PF00069	0.650
MOD_GSK3_1	436	443	PF00069	0.646
MOD_GSK3_1	464	471	PF00069	0.630
MOD_GSK3_1	536	543	PF00069	0.453
MOD_GSK3_1	556	563	PF00069	0.423
MOD_GSK3_1	565	572	PF00069	0.513
MOD_GSK3_1	587	594	PF00069	0.462
MOD_N-GLC_1	238	243	PF02516	0.187
MOD_N-GLC_1	536	541	PF02516	0.321
MOD_N-GLC_1	656	661	PF02516	0.500
MOD_N-GLC_1	674	679	PF02516	0.545
MOD_N-GLC_2	480	482	PF02516	0.285
MOD_NEK2_1	2	7	PF00069	0.771
MOD_NEK2_1	278	283	PF00069	0.507
MOD_NEK2_1	399	404	PF00069	0.472
MOD_NEK2_1	410	415	PF00069	0.425
MOD_NEK2_1	445	450	PF00069	0.540
MOD_NEK2_1	466	471	PF00069	0.520
MOD_NEK2_1	500	505	PF00069	0.466
MOD_NEK2_1	515	520	PF00069	0.453
MOD_NEK2_1	556	561	PF00069	0.543
MOD_NEK2_1	656	661	PF00069	0.481
MOD_NEK2_2	613	618	PF00069	0.300
MOD_PIKK_1	133	139	PF00454	0.610
MOD_PIKK_1	295	301	PF00454	0.428
MOD_PIKK_1	50	56	PF00454	0.477
MOD_PIKK_1	531	537	PF00454	0.531
MOD_PIKK_1	90	96	PF00454	0.620
MOD_PK_1	85	91	PF00069	0.554
MOD_PKA_1	85	91	PF00069	0.556
MOD_PKA_2	317	323	PF00069	0.439
MOD_PKA_2	440	446	PF00069	0.501
MOD_PKA_2	681	687	PF00069	0.646
MOD_PKA_2	85	91	PF00069	0.765
MOD_Plk_1	536	542	PF00069	0.483
MOD_Plk_1	656	662	PF00069	0.522
MOD_Plk_2-3	587	593	PF00069	0.451
MOD_Plk_4	129	135	PF00069	0.463
MOD_Plk_4	182	188	PF00069	0.418
MOD_Plk_4	218	224	PF00069	0.425
MOD_Plk_4	342	348	PF00069	0.435
MOD_Plk_4	359	365	PF00069	0.491
MOD_Plk_4	561	567	PF00069	0.537
MOD_Plk_4	591	597	PF00069	0.405
MOD_Plk_4	613	619	PF00069	0.366
MOD_Plk_4	648	654	PF00069	0.488
MOD_Plk_4	656	662	PF00069	0.496
MOD_ProDKin_1	127	133	PF00069	0.505
MOD_ProDKin_1	148	154	PF00069	0.316
MOD_ProDKin_1	339	345	PF00069	0.482
MOD_ProDKin_1	426	432	PF00069	0.472
MOD_ProDKin_1	446	452	PF00069	0.724
MOD_ProDKin_1	674	680	PF00069	0.682
TRG_DiLeu_BaEn_1	284	289	PF01217	0.429
TRG_DiLeu_BaLyEn_6	393	398	PF01217	0.429
TRG_ENDOCYTIC_2	101	104	PF00928	0.415
TRG_ENDOCYTIC_2	147	150	PF00928	0.527
TRG_ENDOCYTIC_2	164	167	PF00928	0.593
TRG_ENDOCYTIC_2	183	186	PF00928	0.608
TRG_ENDOCYTIC_2	235	238	PF00928	0.439
TRG_ENDOCYTIC_2	408	411	PF00928	0.369
TRG_ENDOCYTIC_2	615	618	PF00928	0.349
TRG_ER_diArg_1	144	147	PF00400	0.493
TRG_ER_diArg_1	357	359	PF00400	0.456
TRG_ER_diArg_1	642	645	PF00400	0.382
TRG_ER_diArg_1	685	687	PF00400	0.681
TRG_NLS_MonoExtN_4	144	149	PF00514	0.378
TRG_Pf-PMV_PEXEL_1	273	277	PF00026	0.187
TRG_Pf-PMV_PEXEL_1	305	310	PF00026	0.336

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I458	Leptomonas seymouri	75%	100%
A0A0N1I5R1	Leptomonas seymouri	22%	88%
A0A0S4IX79	Bodo saltans	24%	96%
A0A0S4JPF4	Bodo saltans	39%	100%
A0A1X0P7J3	Trypanosomatidae	57%	100%
A0A3Q8ID91	Leishmania donovani	26%	100%
A0A3Q8IF94	Leishmania donovani	25%	100%
A0A3Q8IG57	Leishmania donovani	94%	90%
A0A3S7WR01	Leishmania donovani	23%	90%
A4HGR1	Leishmania braziliensis	27%	100%
A4HK20	Leishmania braziliensis	25%	100%
A4HPB9	Leishmania braziliensis	87%	98%
A4HUC1	Leishmania infantum	23%	90%
A4I3T6	Leishmania infantum	26%	100%
A4I7K4	Leishmania infantum	25%	100%
A4ICF9	Leishmania infantum	95%	100%
D0A3C9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	100%
E9AN20	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	95%
E9AT30	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
E9B028	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9B2G1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
Q4Q5P5	Leishmania major	25%	100%
Q4Q858	Leishmania major	26%	100%
V5D8J9	Trypanosoma cruzi	57%	100%