Putative C-4 sterol methyl oxidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

C-4 sterol methyl oxidase, putative

Species:

Leishmania major

UniProt:

Q4Q1G7_LEIMA

TriTrypDb:

LmjF.36.2540 , LMJLV39_360033400 , LMJSD75_360033400

Length:

402

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005789	endoplasmic reticulum membrane	4	2
GO:0016020	membrane	2	7
GO:0031090	organelle membrane	3	2
GO:0110165	cellular anatomical entity	1	7

Expansion

Sequence features

Q4Q1G7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q1G7

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	8
GO:0006694	steroid biosynthetic process	5	2
GO:0008152	metabolic process	1	8
GO:0008202	steroid metabolic process	4	2
GO:0008610	lipid biosynthetic process	4	8
GO:0009058	biosynthetic process	2	8
GO:0016125	sterol metabolic process	4	2
GO:0016126	sterol biosynthetic process	5	2
GO:0044238	primary metabolic process	2	8
GO:0071704	organic substance metabolic process	2	8
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901362	organic cyclic compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	8
GO:1901615	organic hydroxy compound metabolic process	3	2
GO:1901617	organic hydroxy compound biosynthetic process	4	2

Molecular functions
Term	Name	Level	Count
GO:0000254	C-4 methylsterol oxidase activity	5	2
GO:0003824	catalytic activity	1	8
GO:0004497	monooxygenase activity	3	2
GO:0005488	binding	1	8
GO:0005506	iron ion binding	6	8
GO:0016491	oxidoreductase activity	2	8
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	2
GO:0016716	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, another compound as one donor, and incorporation of one atom of oxygen	4	2
GO:0043167	ion binding	2	8
GO:0043169	cation binding	3	8
GO:0046872	metal ion binding	4	8
GO:0046914	transition metal ion binding	5	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	115	117	PF00675	0.429
CLV_NRD_NRD_1	123	125	PF00675	0.379
CLV_PCSK_KEX2_1	115	117	PF00082	0.415
CLV_PCSK_SKI1_1	52	56	PF00082	0.580
CLV_PCSK_SKI1_1	6	10	PF00082	0.558
DEG_MDM2_SWIB_1	316	324	PF02201	0.407
DOC_CKS1_1	251	256	PF01111	0.407
DOC_CYCLIN_yCln2_LP_2	148	154	PF00134	0.430
DOC_CYCLIN_yCln2_LP_2	63	69	PF00134	0.414
DOC_PP2B_LxvP_1	109	112	PF13499	0.600
DOC_PP2B_LxvP_1	148	151	PF13499	0.430
DOC_PP2B_LxvP_1	166	169	PF13499	0.408
DOC_USP7_MATH_1	281	285	PF00917	0.356
DOC_USP7_UBL2_3	121	125	PF12436	0.662
DOC_USP7_UBL2_3	5	9	PF12436	0.680
DOC_WW_Pin1_4	224	229	PF00397	0.333
DOC_WW_Pin1_4	250	255	PF00397	0.356
LIG_14-3-3_CanoR_1	17	25	PF00244	0.607
LIG_14-3-3_CanoR_1	62	66	PF00244	0.404
LIG_14-3-3_CterR_2	399	402	PF00244	0.508
LIG_Actin_WH2_2	180	195	PF00022	0.461
LIG_APCC_ABBA_1	88	93	PF00400	0.445
LIG_BRCT_BRCA1_1	347	351	PF00533	0.356
LIG_CtBP_PxDLS_1	196	200	PF00389	0.452
LIG_EH1_1	217	225	PF00400	0.342
LIG_FHA_1	240	246	PF00498	0.324
LIG_FHA_1	305	311	PF00498	0.407
LIG_FHA_1	338	344	PF00498	0.317
LIG_FHA_1	36	42	PF00498	0.458
LIG_FHA_1	372	378	PF00498	0.548
LIG_FHA_2	251	257	PF00498	0.407
LIG_FHA_2	275	281	PF00498	0.377
LIG_LIR_Apic_2	250	254	PF02991	0.356
LIG_LIR_Gen_1	266	276	PF02991	0.404
LIG_LIR_Gen_1	298	308	PF02991	0.356
LIG_LIR_Gen_1	35	45	PF02991	0.355
LIG_LIR_Gen_1	70	81	PF02991	0.393
LIG_LIR_Nem_3	214	220	PF02991	0.317
LIG_LIR_Nem_3	229	234	PF02991	0.301
LIG_LIR_Nem_3	265	271	PF02991	0.344
LIG_LIR_Nem_3	298	304	PF02991	0.356
LIG_LIR_Nem_3	35	40	PF02991	0.355
LIG_LIR_Nem_3	360	365	PF02991	0.434
LIG_LIR_Nem_3	70	76	PF02991	0.399
LIG_OCRL_FandH_1	233	245	PF00620	0.356
LIG_Pex14_2	269	273	PF04695	0.356
LIG_Pex14_2	316	320	PF04695	0.356
LIG_Pex14_2	69	73	PF04695	0.430
LIG_Pex14_2	85	89	PF04695	0.430
LIG_PTB_Apo_2	83	90	PF02174	0.430
LIG_SH2_CRK	120	124	PF00017	0.657
LIG_SH2_CRK	155	159	PF00017	0.501
LIG_SH2_CRK	251	255	PF00017	0.317
LIG_SH2_CRK	50	54	PF00017	0.427
LIG_SH2_SRC	147	150	PF00017	0.268
LIG_SH2_STAP1	155	159	PF00017	0.501
LIG_SH2_STAT5	147	150	PF00017	0.430
LIG_SH2_STAT5	157	160	PF00017	0.425
LIG_SH2_STAT5	218	221	PF00017	0.317
LIG_SH2_STAT5	241	244	PF00017	0.317
LIG_SH2_STAT5	268	271	PF00017	0.356
LIG_SH2_STAT5	276	279	PF00017	0.356
LIG_SH2_STAT5	30	33	PF00017	0.430
LIG_SH2_STAT5	314	317	PF00017	0.293
LIG_SH2_STAT5	362	365	PF00017	0.386
LIG_SH2_STAT5	59	62	PF00017	0.445
LIG_SH3_1	120	126	PF00018	0.609
LIG_SH3_3	120	126	PF00018	0.600
LIG_SUMO_SIM_par_1	195	201	PF11976	0.394
LIG_TRAF2_1	277	280	PF00917	0.407
LIG_TYR_ITIM	153	158	PF00017	0.387
LIG_WRC_WIRS_1	268	273	PF05994	0.296
MOD_CK1_1	198	204	PF00069	0.365
MOD_CK1_1	226	232	PF00069	0.342
MOD_CK1_1	282	288	PF00069	0.333
MOD_CK2_1	250	256	PF00069	0.356
MOD_CK2_1	274	280	PF00069	0.407
MOD_Cter_Amidation	113	116	PF01082	0.453
MOD_GlcNHglycan	130	133	PF01048	0.429
MOD_GlcNHglycan	172	175	PF01048	0.545
MOD_GlcNHglycan	187	190	PF01048	0.670
MOD_GlcNHglycan	20	23	PF01048	0.351
MOD_GlcNHglycan	288	291	PF01048	0.595
MOD_GlcNHglycan	311	314	PF01048	0.517
MOD_GlcNHglycan	347	350	PF01048	0.556
MOD_GSK3_1	135	142	PF00069	0.446
MOD_GSK3_1	219	226	PF00069	0.407
MOD_GSK3_1	239	246	PF00069	0.360
MOD_GSK3_1	263	270	PF00069	0.240
MOD_GSK3_1	281	288	PF00069	0.329
MOD_N-GLC_2	162	164	PF02516	0.650
MOD_N-GLC_2	342	344	PF02516	0.607
MOD_NEK2_1	134	139	PF00069	0.444
MOD_NEK2_1	158	163	PF00069	0.511
MOD_NEK2_1	187	192	PF00069	0.387
MOD_NEK2_1	223	228	PF00069	0.355
MOD_NEK2_1	286	291	PF00069	0.346
MOD_NEK2_1	295	300	PF00069	0.251
MOD_NEK2_1	316	321	PF00069	0.296
MOD_NEK2_1	337	342	PF00069	0.351
MOD_NEK2_1	55	60	PF00069	0.414
MOD_NEK2_1	91	96	PF00069	0.617
MOD_PKA_2	61	67	PF00069	0.376
MOD_Plk_4	135	141	PF00069	0.446
MOD_Plk_4	219	225	PF00069	0.374
MOD_Plk_4	226	232	PF00069	0.333
MOD_Plk_4	24	30	PF00069	0.377
MOD_Plk_4	282	288	PF00069	0.290
MOD_Plk_4	316	322	PF00069	0.356
MOD_Plk_4	41	47	PF00069	0.350
MOD_Plk_4	55	61	PF00069	0.467
MOD_ProDKin_1	224	230	PF00069	0.333
MOD_ProDKin_1	250	256	PF00069	0.356
MOD_SUMO_for_1	377	380	PF00179	0.457
MOD_SUMO_rev_2	379	385	PF00179	0.539
TRG_DiLeu_BaLyEn_6	49	54	PF01217	0.413
TRG_ENDOCYTIC_2	147	150	PF00928	0.430
TRG_ENDOCYTIC_2	155	158	PF00928	0.387
TRG_ENDOCYTIC_2	231	234	PF00928	0.313
TRG_ENDOCYTIC_2	268	271	PF00928	0.349
TRG_ENDOCYTIC_2	50	53	PF00928	0.371
TRG_ER_diArg_1	115	117	PF00400	0.618
TRG_ER_diArg_1	331	334	PF00400	0.356
TRG_Pf-PMV_PEXEL_1	52	56	PF00026	0.664

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEE3	Leptomonas seymouri	57%	100%
A0A0S4JXU3	Bodo saltans	37%	100%
A0A3Q8IKR9	Leishmania donovani	94%	100%
A4HPC0	Leishmania braziliensis	76%	100%
A4ICG0	Leishmania infantum	94%	100%
E9AT31	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	100%