Putative pre-mRNA branch site protein p14

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

pre-mRNA branch site protein p14, putative

Species:

Leishmania major

UniProt:

Q4Q1B5_LEIMA

TriTrypDb:

LmjF.36.3040 , LMJLV39_360039800 , LMJSD75_360039700

Length:

183

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 4
NetGPI	no	yes: 0, no: 4

Cellular components
Term	Name	Level	Count
GO:0005681	spliceosomal complex	3	2
GO:0005684	U2-type spliceosomal complex	4	2
GO:0005686	U2 snRNP	5	2
GO:0030532	small nuclear ribonucleoprotein complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0071011	precatalytic spliceosome	4	2
GO:0071013	catalytic step 2 spliceosome	3	2
GO:0097525	spliceosomal snRNP complex	4	2
GO:0120114	Sm-like protein family complex	2	2
GO:0140513	nuclear protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:1990904	ribonucleoprotein complex	2	2

Expansion

Sequence features

Q4Q1B5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q1B5

Function

Biological processes
Term	Name	Level	Count
GO:0000375	RNA splicing, via transesterification reactions	8	2
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	2
GO:0000398	mRNA splicing, via spliceosome	8	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006397	mRNA processing	7	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0008380	RNA splicing	7	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0016071	mRNA metabolic process	6	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	5
GO:0003723	RNA binding	4	5
GO:0005488	binding	1	5
GO:0097159	organic cyclic compound binding	2	5
GO:1901363	heterocyclic compound binding	2	5

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	156	158	PF00675	0.539
CLV_NRD_NRD_1	17	19	PF00675	0.512
CLV_PCSK_FUR_1	156	160	PF00082	0.539
CLV_PCSK_KEX2_1	156	158	PF00082	0.607
CLV_PCSK_KEX2_1	160	162	PF00082	0.455
CLV_PCSK_KEX2_1	17	19	PF00082	0.512
CLV_PCSK_PC1ET2_1	158	160	PF00082	0.561
CLV_PCSK_PC7_1	13	19	PF00082	0.509
CLV_PCSK_PC7_1	156	162	PF00082	0.533
CLV_PCSK_SKI1_1	151	155	PF00082	0.648
CLV_PCSK_SKI1_1	70	74	PF00082	0.440
DEG_APCC_DBOX_1	17	25	PF00400	0.428
DEG_Nend_UBRbox_1	1	4	PF02207	0.515
DOC_MAPK_DCC_7	33	42	PF00069	0.407
DOC_MAPK_gen_1	136	144	PF00069	0.516
DOC_MAPK_gen_1	2	10	PF00069	0.453
DOC_MAPK_HePTP_8	30	42	PF00069	0.408
DOC_MAPK_MEF2A_6	136	144	PF00069	0.481
DOC_MAPK_MEF2A_6	33	42	PF00069	0.422
DOC_WW_Pin1_4	52	57	PF00397	0.461
LIG_14-3-3_CanoR_1	141	145	PF00244	0.461
LIG_14-3-3_CanoR_1	17	25	PF00244	0.503
LIG_14-3-3_CanoR_1	5	11	PF00244	0.466
LIG_APCC_ABBA_1	142	147	PF00400	0.472
LIG_APCC_ABBAyCdc20_2	141	147	PF00400	0.475
LIG_FHA_1	27	33	PF00498	0.529
LIG_LIR_Gen_1	85	94	PF02991	0.525
LIG_LIR_Nem_3	85	91	PF02991	0.525
LIG_MAD2	70	78	PF02301	0.375
LIG_NRBOX	20	26	PF00104	0.474
LIG_NRBOX	27	33	PF00104	0.488
LIG_SH2_CRK	94	98	PF00017	0.525
LIG_SH2_SRC	86	89	PF00017	0.525
LIG_SH2_STAP1	45	49	PF00017	0.489
LIG_SH2_STAT5	86	89	PF00017	0.525
LIG_SH3_3	32	38	PF00018	0.477
LIG_SUMO_SIM_anti_2	68	75	PF11976	0.455
LIG_SUMO_SIM_par_1	68	75	PF11976	0.455
LIG_SxIP_EBH_1	70	83	PF03271	0.375
LIG_UBA3_1	24	33	PF00899	0.418
MOD_CK1_1	105	111	PF00069	0.525
MOD_CK1_1	52	58	PF00069	0.552
MOD_CK2_1	140	146	PF00069	0.462
MOD_GlcNHglycan	45	48	PF01048	0.506
MOD_GSK3_1	101	108	PF00069	0.525
MOD_GSK3_1	45	52	PF00069	0.501
MOD_GSK3_1	72	79	PF00069	0.300
MOD_N-GLC_1	57	62	PF02516	0.513
MOD_NEK2_1	140	145	PF00069	0.455
MOD_NEK2_1	26	31	PF00069	0.475
MOD_NEK2_1	43	48	PF00069	0.408
MOD_NEK2_1	6	11	PF00069	0.437
MOD_NEK2_1	72	77	PF00069	0.375
MOD_NEK2_2	86	91	PF00069	0.525
MOD_PKA_2	140	146	PF00069	0.462
MOD_PKA_2	16	22	PF00069	0.506
MOD_PKA_2	6	12	PF00069	0.476
MOD_Plk_1	57	63	PF00069	0.517
MOD_Plk_4	140	146	PF00069	0.462
MOD_Plk_4	26	32	PF00069	0.497
MOD_Plk_4	45	51	PF00069	0.456
MOD_Plk_4	86	92	PF00069	0.525
MOD_ProDKin_1	52	58	PF00069	0.465
MOD_SUMO_rev_2	146	154	PF00179	0.556
TRG_DiLeu_BaEn_1	69	74	PF01217	0.446
TRG_DiLeu_BaLyEn_6	35	40	PF01217	0.476
TRG_ENDOCYTIC_2	88	91	PF00928	0.525
TRG_ENDOCYTIC_2	94	97	PF00928	0.487
TRG_ER_diArg_1	156	159	PF00400	0.615
TRG_NLS_MonoCore_2	156	161	PF00514	0.537
TRG_NLS_MonoExtC_3	156	161	PF00514	0.537
TRG_NLS_MonoExtN_4	156	161	PF00514	0.537

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A3Q8IHF1	Leishmania donovani	91%	100%
A4HPH0	Leishmania braziliensis	86%	100%
A4ID92	Leishmania infantum	91%	100%