Vacuolar protein sorting-associated protein-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

vacuolar protein sorting-associated protein-like protein

Species:

Leishmania major

UniProt:

Q4Q176_LEIMA

TriTrypDb:

LmjF.36.3420 , LMJLV39_360044300 , LMJSD75_360044200

Length:

361

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005768	endosome	7	2
GO:0005829	cytosol	2	2
GO:0030904	retromer complex	3	2
GO:0031410	cytoplasmic vesicle	6	2
GO:0031982	vesicle	4	2
GO:0032991	protein-containing complex	1	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097708	intracellular vesicle	5	2
GO:0098796	membrane protein complex	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q176

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q176

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	11
GO:0006886	intracellular protein transport	4	11
GO:0008104	protein localization	4	11
GO:0009987	cellular process	1	11
GO:0015031	protein transport	4	11
GO:0016192	vesicle-mediated transport	4	2
GO:0016197	endosomal transport	4	2
GO:0016482	cytosolic transport	4	2
GO:0033036	macromolecule localization	2	11
GO:0042147	retrograde transport, endosome to Golgi	5	2
GO:0045184	establishment of protein localization	3	11
GO:0046907	intracellular transport	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0051649	establishment of localization in cell	3	11
GO:0070727	cellular macromolecule localization	3	11
GO:0071702	organic substance transport	4	11
GO:0071705	nitrogen compound transport	4	11

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	174	178	PF00656	0.290
CLV_C14_Caspase3-7	266	270	PF00656	0.273
CLV_NRD_NRD_1	243	245	PF00675	0.273
CLV_NRD_NRD_1	329	331	PF00675	0.382
CLV_PCSK_KEX2_1	317	319	PF00082	0.364
CLV_PCSK_KEX2_1	329	331	PF00082	0.369
CLV_PCSK_PC1ET2_1	317	319	PF00082	0.380
CLV_PCSK_SKI1_1	100	104	PF00082	0.449
CLV_PCSK_SKI1_1	213	217	PF00082	0.273
CLV_PCSK_SKI1_1	245	249	PF00082	0.382
CLV_PCSK_SKI1_1	304	308	PF00082	0.385
CLV_PCSK_SKI1_1	318	322	PF00082	0.293
CLV_PCSK_SKI1_1	335	339	PF00082	0.505
DEG_APCC_DBOX_1	280	288	PF00400	0.273
DEG_APCC_DBOX_1	303	311	PF00400	0.391
DEG_Nend_Nbox_1	1	3	PF02207	0.573
DEG_SCF_FBW7_1	69	76	PF00400	0.351
DEG_SCF_FBW7_2	268	275	PF00400	0.391
DOC_CDC14_PxL_1	284	292	PF14671	0.273
DOC_CKS1_1	117	122	PF01111	0.391
DOC_CKS1_1	345	350	PF01111	0.476
DOC_CKS1_1	70	75	PF01111	0.317
DOC_MAPK_gen_1	317	327	PF00069	0.361
DOC_MAPK_MEF2A_6	304	311	PF00069	0.327
DOC_MAPK_NFAT4_5	304	312	PF00069	0.391
DOC_PP1_RVXF_1	98	104	PF00149	0.311
DOC_PP4_FxxP_1	125	128	PF00568	0.317
DOC_PP4_FxxP_1	247	250	PF00568	0.360
DOC_USP7_MATH_1	73	77	PF00917	0.351
DOC_USP7_MATH_2	128	134	PF00917	0.317
DOC_USP7_UBL2_3	150	154	PF12436	0.412
DOC_USP7_UBL2_3	225	229	PF12436	0.345
DOC_USP7_UBL2_3	317	321	PF12436	0.389
DOC_USP7_UBL2_3	62	66	PF12436	0.391
DOC_WW_Pin1_4	116	121	PF00397	0.351
DOC_WW_Pin1_4	152	157	PF00397	0.359
DOC_WW_Pin1_4	268	273	PF00397	0.287
DOC_WW_Pin1_4	288	293	PF00397	0.273
DOC_WW_Pin1_4	344	349	PF00397	0.490
DOC_WW_Pin1_4	69	74	PF00397	0.293
LIG_14-3-3_CanoR_1	335	341	PF00244	0.633
LIG_Actin_WH2_2	270	287	PF00022	0.269
LIG_BIR_III_4	354	358	PF00653	0.475
LIG_BRCT_BRCA1_1	117	121	PF00533	0.250
LIG_BRCT_BRCA1_1	52	56	PF00533	0.399
LIG_CaMK_CASK_1	160	166	PF00069	0.350
LIG_eIF4E_1	237	243	PF01652	0.399
LIG_FHA_1	269	275	PF00498	0.292
LIG_FHA_2	117	123	PF00498	0.273
LIG_FHA_2	329	335	PF00498	0.504
LIG_FHA_2	36	42	PF00498	0.311
LIG_FHA_2	91	97	PF00498	0.317
LIG_LIR_Apic_2	122	128	PF02991	0.317
LIG_LIR_Apic_2	246	250	PF02991	0.317
LIG_LIR_Apic_2	60	64	PF02991	0.399
LIG_LIR_Gen_1	16	25	PF02991	0.580
LIG_LIR_Gen_1	291	301	PF02991	0.399
LIG_LIR_Gen_1	47	57	PF02991	0.441
LIG_LIR_Nem_3	132	137	PF02991	0.241
LIG_LIR_Nem_3	16	21	PF02991	0.632
LIG_LIR_Nem_3	234	240	PF02991	0.293
LIG_LIR_Nem_3	291	297	PF02991	0.273
LIG_LIR_Nem_3	47	52	PF02991	0.442
LIG_LIR_Nem_3	53	59	PF02991	0.465
LIG_LIR_Nem_3	92	97	PF02991	0.387
LIG_MYND_1	288	292	PF01753	0.273
LIG_PCNA_yPIPBox_3	198	210	PF02747	0.391
LIG_PCNA_yPIPBox_3	81	95	PF02747	0.353
LIG_Pex14_2	121	125	PF04695	0.399
LIG_SH2_CRK	134	138	PF00017	0.317
LIG_SH2_CRK	49	53	PF00017	0.399
LIG_SH2_NCK_1	49	53	PF00017	0.399
LIG_SH2_STAP1	211	215	PF00017	0.273
LIG_SH2_STAT3	143	146	PF00017	0.391
LIG_SH2_STAT3	319	322	PF00017	0.383
LIG_SH2_STAT3	78	81	PF00017	0.391
LIG_SH2_STAT5	143	146	PF00017	0.276
LIG_SH2_STAT5	184	187	PF00017	0.293
LIG_SH2_STAT5	283	286	PF00017	0.273
LIG_SH2_STAT5	305	308	PF00017	0.292
LIG_SH2_STAT5	319	322	PF00017	0.382
LIG_SH3_3	342	348	PF00018	0.509
LIG_SH3_3	67	73	PF00018	0.350
LIG_SUMO_SIM_par_1	44	50	PF11976	0.342
LIG_TRAF2_1	331	334	PF00917	0.453
LIG_TYR_ITIM	235	240	PF00017	0.293
LIG_UBA3_1	21	29	PF00899	0.467
MOD_CDK_SPxxK_3	152	159	PF00069	0.359
MOD_CK1_1	16	22	PF00069	0.616
MOD_CK1_1	336	342	PF00069	0.464
MOD_CK1_1	76	82	PF00069	0.391
MOD_CK2_1	116	122	PF00069	0.273
MOD_CK2_1	154	160	PF00069	0.315
MOD_CK2_1	328	334	PF00069	0.428
MOD_CK2_1	57	63	PF00069	0.293
MOD_CK2_1	90	96	PF00069	0.294
MOD_Cter_Amidation	315	318	PF01082	0.377
MOD_GlcNHglycan	125	128	PF01048	0.357
MOD_GlcNHglycan	177	181	PF01048	0.368
MOD_GlcNHglycan	334	338	PF01048	0.396
MOD_GlcNHglycan	341	344	PF01048	0.626
MOD_GlcNHglycan	349	352	PF01048	0.512
MOD_GSK3_1	111	118	PF00069	0.387
MOD_GSK3_1	169	176	PF00069	0.234
MOD_GSK3_1	335	342	PF00069	0.567
MOD_GSK3_1	69	76	PF00069	0.351
MOD_N-GLC_1	115	120	PF02516	0.232
MOD_NEK2_1	115	120	PF00069	0.308
MOD_NEK2_1	121	126	PF00069	0.288
MOD_NEK2_1	171	176	PF00069	0.284
MOD_NEK2_1	185	190	PF00069	0.273
MOD_PIKK_1	171	177	PF00454	0.273
MOD_PK_1	154	160	PF00069	0.391
MOD_PKA_2	185	191	PF00069	0.273
MOD_PKA_2	328	334	PF00069	0.540
MOD_Plk_1	121	127	PF00069	0.210
MOD_Plk_1	253	259	PF00069	0.200
MOD_Plk_2-3	35	41	PF00069	0.371
MOD_Plk_4	290	296	PF00069	0.391
MOD_ProDKin_1	116	122	PF00069	0.351
MOD_ProDKin_1	152	158	PF00069	0.359
MOD_ProDKin_1	268	274	PF00069	0.287
MOD_ProDKin_1	288	294	PF00069	0.273
MOD_ProDKin_1	344	350	PF00069	0.487
MOD_ProDKin_1	69	75	PF00069	0.293
MOD_SUMO_for_1	158	161	PF00179	0.391
MOD_SUMO_rev_2	208	216	PF00179	0.293
TRG_ENDOCYTIC_2	134	137	PF00928	0.293
TRG_ENDOCYTIC_2	138	141	PF00928	0.293
TRG_ENDOCYTIC_2	237	240	PF00928	0.293
TRG_ENDOCYTIC_2	294	297	PF00928	0.273
TRG_ENDOCYTIC_2	49	52	PF00928	0.410
TRG_ER_diArg_1	140	143	PF00400	0.327
TRG_ER_diArg_1	328	330	PF00400	0.357

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6N0	Leptomonas seymouri	74%	100%
A0A1X0P3C6	Trypanosomatidae	59%	100%
A0A3Q8IW72	Leishmania donovani	96%	100%
A0A422NA47	Trypanosoma rangeli	60%	100%
A4HPK9	Leishmania braziliensis	85%	100%
A4ID55	Leishmania infantum	96%	100%
D0A8K4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	100%
E9ATC1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
O01258	Caenorhabditis elegans	37%	100%
O75436	Homo sapiens	34%	100%
P40335	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	95%
P40336	Mus musculus	34%	100%
Q0VD53	Bos taurus	34%	100%
Q10243	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	33%	100%
Q28HT6	Xenopus tropicalis	36%	100%
Q4G0F5	Homo sapiens	32%	100%
Q55CA0	Dictyostelium discoideum	37%	100%
Q5BKM4	Xenopus tropicalis	33%	100%
Q5R436	Pongo abelii	32%	100%
Q68F29	Xenopus laevis	33%	100%
Q6AY86	Rattus norvegicus	34%	100%
Q6DFB9	Xenopus laevis	33%	100%
Q6DFU4	Xenopus laevis	35%	100%
Q6DH23	Danio rerio	33%	100%
Q6IRD0	Xenopus laevis	34%	100%
Q6TNP8	Danio rerio	34%	100%
Q7ZV03	Danio rerio	32%	100%
Q8C0E2	Mus musculus	31%	100%
Q8I4T1	Plasmodium falciparum (isolate 3D7)	34%	100%
Q9FJD0	Arabidopsis thaliana	34%	100%
Q9T091	Arabidopsis thaliana	35%	100%
Q9W552	Drosophila melanogaster	35%	76%
V5BMH4	Trypanosoma cruzi	59%	100%