Glycine--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

glycyl tRNA synthetase, putative

Species:

Leishmania major

UniProt:

Q4Q131_LEIMA

TriTrypDb:

LmjF.36.3840 , LMJLV39_360049100 , LMJSD75_360048900

Length:

628

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	yes	yes: 2
Pissara et al.	yes	yes: 8

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 4

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 4

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0005739	mitochondrion	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

Q4Q131

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q131

Function

Biological processes
Term	Name	Level	Count
GO:0000959	mitochondrial RNA metabolic process	6	2
GO:0006082	organic acid metabolic process	3	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006399	tRNA metabolic process	7	12
GO:0006418	tRNA aminoacylation for protein translation	6	12
GO:0006426	glycyl-tRNA aminoacylation	7	12
GO:0006520	amino acid metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0019752	carboxylic acid metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043038	amino acid activation	4	12
GO:0043039	tRNA aminoacylation	5	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043436	oxoacid metabolic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044281	small molecule metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0070127	tRNA aminoacylation for mitochondrial protein translation	7	2
GO:0070150	mitochondrial glycyl-tRNA aminoacylation	8	2
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901564	organonitrogen compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004812	aminoacyl-tRNA ligase activity	4	12
GO:0004820	glycine-tRNA ligase activity	5	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	25	27	PF00675	0.600
CLV_NRD_NRD_1	490	492	PF00675	0.442
CLV_PCSK_KEX2_1	203	205	PF00082	0.416
CLV_PCSK_KEX2_1	24	26	PF00082	0.604
CLV_PCSK_KEX2_1	490	492	PF00082	0.442
CLV_PCSK_KEX2_1	499	501	PF00082	0.419
CLV_PCSK_PC1ET2_1	203	205	PF00082	0.416
CLV_PCSK_PC1ET2_1	499	501	PF00082	0.419
CLV_PCSK_SKI1_1	150	154	PF00082	0.678
CLV_PCSK_SKI1_1	232	236	PF00082	0.418
CLV_PCSK_SKI1_1	239	243	PF00082	0.368
CLV_PCSK_SKI1_1	25	29	PF00082	0.586
CLV_PCSK_SKI1_1	307	311	PF00082	0.388
CLV_PCSK_SKI1_1	386	390	PF00082	0.496
CLV_PCSK_SKI1_1	455	459	PF00082	0.430
CLV_PCSK_SKI1_1	500	504	PF00082	0.402
CLV_PCSK_SKI1_1	513	517	PF00082	0.360
DEG_APCC_DBOX_1	231	239	PF00400	0.602
DEG_APCC_DBOX_1	385	393	PF00400	0.706
DEG_ODPH_VHL_1	509	521	PF01847	0.560
DOC_CYCLIN_RxL_1	383	393	PF00134	0.708
DOC_CYCLIN_yCln2_LP_2	562	568	PF00134	0.549
DOC_CYCLIN_yCln2_LP_2	603	609	PF00134	0.674
DOC_MAPK_DCC_7	513	521	PF00069	0.549
DOC_MAPK_gen_1	24	33	PF00069	0.391
DOC_MAPK_gen_1	259	268	PF00069	0.627
DOC_MAPK_gen_1	554	564	PF00069	0.549
DOC_MAPK_MEF2A_6	513	521	PF00069	0.574
DOC_MAPK_MEF2A_6	558	566	PF00069	0.549
DOC_PP2B_LxvP_1	508	511	PF13499	0.587
DOC_PP2B_LxvP_1	562	565	PF13499	0.549
DOC_PP4_FxxP_1	166	169	PF00568	0.478
DOC_PP4_FxxP_1	249	252	PF00568	0.599
DOC_PP4_MxPP_1	144	147	PF00568	0.363
DOC_USP7_MATH_1	2	6	PF00917	0.544
DOC_USP7_MATH_1	355	359	PF00917	0.591
DOC_USP7_MATH_1	436	440	PF00917	0.700
DOC_USP7_MATH_1	51	55	PF00917	0.470
DOC_USP7_UBL2_3	155	159	PF12436	0.469
DOC_USP7_UBL2_3	255	259	PF12436	0.616
DOC_USP7_UBL2_3	399	403	PF12436	0.649
DOC_WW_Pin1_4	157	162	PF00397	0.441
DOC_WW_Pin1_4	462	467	PF00397	0.685
DOC_WW_Pin1_4	503	508	PF00397	0.644
DOC_WW_Pin1_4	76	81	PF00397	0.407
LIG_14-3-3_CanoR_1	323	333	PF00244	0.721
LIG_14-3-3_CanoR_1	500	506	PF00244	0.598
LIG_14-3-3_CanoR_1	539	544	PF00244	0.635
LIG_AP2alpha_1	166	170	PF02296	0.478
LIG_AP2alpha_2	168	170	PF02296	0.553
LIG_BIR_III_4	140	144	PF00653	0.528
LIG_BRCT_BRCA1_1	166	170	PF00533	0.401
LIG_deltaCOP1_diTrp_1	267	275	PF00928	0.605
LIG_EH_1	163	167	PF12763	0.456
LIG_EH1_1	472	480	PF00400	0.574
LIG_FHA_1	37	43	PF00498	0.462
LIG_FHA_1	391	397	PF00498	0.694
LIG_FHA_1	432	438	PF00498	0.671
LIG_FHA_1	463	469	PF00498	0.673
LIG_FHA_1	73	79	PF00498	0.410
LIG_FHA_2	15	21	PF00498	0.449
LIG_FHA_2	240	246	PF00498	0.575
LIG_FHA_2	284	290	PF00498	0.610
LIG_FHA_2	526	532	PF00498	0.603
LIG_FHA_2	577	583	PF00498	0.549
LIG_FHA_2	77	83	PF00498	0.405
LIG_LIR_Apic_2	247	252	PF02991	0.593
LIG_LIR_Apic_2	267	272	PF02991	0.452
LIG_LIR_Apic_2	377	382	PF02991	0.647
LIG_LIR_Gen_1	132	142	PF02991	0.525
LIG_LIR_Gen_1	258	268	PF02991	0.630
LIG_LIR_Gen_1	611	620	PF02991	0.633
LIG_LIR_Gen_1	91	98	PF02991	0.464
LIG_LIR_Nem_3	132	137	PF02991	0.492
LIG_LIR_Nem_3	258	263	PF02991	0.623
LIG_LIR_Nem_3	344	349	PF02991	0.585
LIG_LIR_Nem_3	504	508	PF02991	0.671
LIG_LIR_Nem_3	611	615	PF02991	0.610
LIG_LIR_Nem_3	91	96	PF02991	0.476
LIG_MYND_1	507	511	PF01753	0.649
LIG_MYND_3	591	595	PF01753	0.487
LIG_NRBOX	478	484	PF00104	0.566
LIG_Pex14_1	271	275	PF04695	0.540
LIG_Pex14_1	60	64	PF04695	0.375
LIG_Pex14_2	112	116	PF04695	0.491
LIG_Pex14_2	166	170	PF04695	0.417
LIG_Pex14_2	172	176	PF04695	0.596
LIG_SH2_GRB2like	411	414	PF00017	0.634
LIG_SH2_NCK_1	556	560	PF00017	0.549
LIG_SH2_SRC	379	382	PF00017	0.721
LIG_SH2_SRC	411	414	PF00017	0.659
LIG_SH2_STAT3	619	622	PF00017	0.709
LIG_SH2_STAT5	303	306	PF00017	0.555
LIG_SH2_STAT5	411	414	PF00017	0.621
LIG_SH2_STAT5	464	467	PF00017	0.608
LIG_SH2_STAT5	480	483	PF00017	0.530
LIG_SH3_3	432	438	PF00018	0.706
LIG_SH3_3	465	471	PF00018	0.573
LIG_SH3_3	586	592	PF00018	0.549
LIG_SH3_3	603	609	PF00018	0.613
LIG_SUMO_SIM_par_1	433	439	PF11976	0.719
LIG_TRAF2_1	375	378	PF00917	0.634
LIG_TRAF2_1	439	442	PF00917	0.738
LIG_TRAF2_1	451	454	PF00917	0.705
LIG_TRAF2_1	79	82	PF00917	0.431
LIG_TRFH_1	43	47	PF08558	0.442
LIG_TYR_ITIM	478	483	PF00017	0.555
LIG_TYR_ITSM	342	349	PF00017	0.577
LIG_UBA3_1	388	394	PF00899	0.700
LIG_UBA3_1	516	522	PF00899	0.610
LIG_WRC_WIRS_1	449	454	PF05994	0.695
LIG_WRC_WIRS_1	502	507	PF05994	0.670
MOD_CDK_SPK_2	157	162	PF00069	0.401
MOD_CK1_1	366	372	PF00069	0.638
MOD_CK1_1	548	554	PF00069	0.569
MOD_CK2_1	14	20	PF00069	0.440
MOD_CK2_1	239	245	PF00069	0.574
MOD_CK2_1	283	289	PF00069	0.684
MOD_CK2_1	436	442	PF00069	0.708
MOD_CK2_1	448	454	PF00069	0.639
MOD_CK2_1	464	470	PF00069	0.497
MOD_CK2_1	525	531	PF00069	0.626
MOD_CK2_1	576	582	PF00069	0.549
MOD_CK2_1	66	72	PF00069	0.421
MOD_CK2_1	76	82	PF00069	0.386
MOD_Cter_Amidation	401	404	PF01082	0.505
MOD_Cter_Amidation	552	555	PF01082	0.349
MOD_GlcNHglycan	207	210	PF01048	0.470
MOD_GlcNHglycan	232	235	PF01048	0.517
MOD_GlcNHglycan	356	360	PF01048	0.396
MOD_GlcNHglycan	413	416	PF01048	0.467
MOD_GlcNHglycan	438	441	PF01048	0.490
MOD_GlcNHglycan	86	89	PF01048	0.596
MOD_GSK3_1	460	467	PF00069	0.662
MOD_GSK3_1	72	79	PF00069	0.410
MOD_GSK3_1	84	91	PF00069	0.409
MOD_N-GLC_1	297	302	PF02516	0.376
MOD_N-GLC_1	366	371	PF02516	0.497
MOD_NEK2_1	175	180	PF00069	0.614
MOD_NEK2_1	19	24	PF00069	0.391
MOD_NEK2_1	205	210	PF00069	0.642
MOD_NEK2_1	310	315	PF00069	0.610
MOD_NEK2_1	342	347	PF00069	0.566
MOD_NEK2_1	483	488	PF00069	0.567
MOD_NEK2_1	525	530	PF00069	0.617
MOD_NEK2_1	84	89	PF00069	0.422
MOD_NEK2_2	576	581	PF00069	0.549
MOD_OFUCOSY	71	77	PF10250	0.620
MOD_PIKK_1	360	366	PF00454	0.613
MOD_PIKK_1	584	590	PF00454	0.635
MOD_PK_1	539	545	PF00069	0.635
MOD_PKA_2	283	289	PF00069	0.655
MOD_PKA_2	582	588	PF00069	0.549
MOD_PKA_2	98	104	PF00069	0.413
MOD_PKB_1	237	245	PF00069	0.581
MOD_Plk_1	239	245	PF00069	0.578
MOD_Plk_1	297	303	PF00069	0.573
MOD_Plk_1	355	361	PF00069	0.593
MOD_Plk_1	441	447	PF00069	0.704
MOD_Plk_1	483	489	PF00069	0.571
MOD_Plk_1	545	551	PF00069	0.654
MOD_Plk_1	66	72	PF00069	0.419
MOD_Plk_1	73	79	PF00069	0.391
MOD_Plk_4	464	470	PF00069	0.577
MOD_Plk_4	483	489	PF00069	0.571
MOD_Plk_4	595	601	PF00069	0.653
MOD_ProDKin_1	157	163	PF00069	0.441
MOD_ProDKin_1	462	468	PF00069	0.681
MOD_ProDKin_1	503	509	PF00069	0.641
MOD_ProDKin_1	76	82	PF00069	0.411
MOD_SUMO_for_1	521	524	PF00179	0.549
MOD_SUMO_for_1	592	595	PF00179	0.598
MOD_SUMO_for_1	9	12	PF00179	0.514
MOD_SUMO_rev_2	289	296	PF00179	0.677
MOD_SUMO_rev_2	451	460	PF00179	0.648
TRG_DiLeu_BaEn_1	133	138	PF01217	0.487
TRG_DiLeu_BaEn_2	244	250	PF01217	0.576
TRG_DiLeu_BaLyEn_6	504	509	PF01217	0.607
TRG_ENDOCYTIC_2	346	349	PF00928	0.591
TRG_ENDOCYTIC_2	480	483	PF00928	0.555
TRG_ENDOCYTIC_2	556	559	PF00928	0.549
TRG_ER_diArg_1	236	239	PF00400	0.590
TRG_ER_diArg_1	24	26	PF00400	0.404
TRG_ER_diArg_1	489	491	PF00400	0.620
TRG_Pf-PMV_PEXEL_1	111	115	PF00026	0.622
TRG_Pf-PMV_PEXEL_1	386	391	PF00026	0.528

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZB0	Leptomonas seymouri	85%	95%
A0A0S4JJA5	Bodo saltans	66%	100%
A0A1X0P3R2	Trypanosomatidae	72%	100%
A0A3Q8IPA6	Leishmania donovani	97%	100%
A0A422MT81	Trypanosoma rangeli	70%	100%
A0B5U4	Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT)	37%	100%
A4FZX1	Methanococcus maripaludis (strain C5 / ATCC BAA-1333)	37%	100%
A4HPQ1	Leishmania braziliensis	93%	100%
A4ID12	Leishmania infantum	98%	100%
A6URK3	Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)	37%	100%
A6VIK1	Methanococcus maripaludis (strain C7 / ATCC BAA-1331)	38%	100%
A9A885	Methanococcus maripaludis (strain C6 / ATCC BAA-1332)	38%	100%
C5A1H0	Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)	37%	100%
C6A317	Thermococcus sibiricus (strain DSM 12597 / MM 739)	37%	100%
D0A8G2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	67%	100%
E9ATG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
O23627	Arabidopsis thaliana	48%	86%
O27874	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	40%	100%
O29346	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	39%	100%
O59235	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	36%	100%
P38088	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	44%	91%
P41250	Homo sapiens	44%	85%
P56206	Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)	29%	100%
Q04451	Bombyx mori	44%	86%
Q06817	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	42%	100%
Q10039	Caenorhabditis elegans	44%	85%
Q10179	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	43%	96%
Q57681	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	40%	100%
Q5I0G4	Rattus norvegicus	45%	86%
Q5JID8	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	36%	100%
Q5RBL1	Pongo abelii	45%	85%
Q6M0Q7	Methanococcus maripaludis (strain S2 / LL)	37%	100%
Q72L85	Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27)	28%	100%
Q7UEU9	Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)	27%	100%
Q8SQZ6	Encephalitozoon cuniculi (strain GB-M1)	41%	100%
Q8TYY9	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	37%	100%
Q8U0G2	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	36%	100%
Q9CZD3	Mus musculus	45%	86%
Q9V176	Pyrococcus abyssi (strain GE5 / Orsay)	36%	100%
Q9VUK8	Drosophila melanogaster	45%	82%
Q9YBF8	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	34%	100%
V5AU76	Trypanosoma cruzi	70%	100%