GOLD domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

emp24/gp25L/p24 family/GOLD, putative

Species:

Leishmania major

UniProt:

Q4Q0K4_LEIMA

TriTrypDb:

LmjF.36.5570 , LMJLV39_360067900 , LMJSD75_360067900

Length:

248

Annotations

LeishMANIAdb annotations

Conserved eukaryotic protein. Probable receptor for ER-Golgi trafficking of GPI-anchored proteins. distinct from the other GOLD domain cluster (related to YB79_SCHPO instead). Localization: ER (by homology) / Golgi (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 8, no: 1
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	2
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	2
GO:0005794	Golgi apparatus	5	2
GO:0016020	membrane	2	10
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	2
GO:0030135	coated vesicle	7	2
GO:0031410	cytoplasmic vesicle	6	2
GO:0031982	vesicle	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097708	intracellular vesicle	5	2
GO:0110165	cellular anatomical entity	1	10

Expansion

Sequence features

Q4Q0K4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q0K4

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0006886	intracellular protein transport	4	2
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	2
GO:0006996	organelle organization	4	2
GO:0007030	Golgi organization	5	2
GO:0008104	protein localization	4	2
GO:0009987	cellular process	1	2
GO:0015031	protein transport	4	2
GO:0016043	cellular component organization	3	2
GO:0016192	vesicle-mediated transport	4	2
GO:0033036	macromolecule localization	2	2
GO:0045184	establishment of protein localization	3	2
GO:0046907	intracellular transport	3	2
GO:0048193	Golgi vesicle transport	5	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0070727	cellular macromolecule localization	3	2
GO:0071702	organic substance transport	4	2
GO:0071705	nitrogen compound transport	4	2
GO:0071840	cellular component organization or biogenesis	2	2

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	201	203	PF00675	0.540
CLV_PCSK_KEX2_1	100	102	PF00082	0.660
CLV_PCSK_KEX2_1	201	203	PF00082	0.548
CLV_PCSK_KEX2_1	239	241	PF00082	0.336
CLV_PCSK_PC1ET2_1	100	102	PF00082	0.660
CLV_PCSK_PC1ET2_1	239	241	PF00082	0.336
CLV_PCSK_SKI1_1	202	206	PF00082	0.561
CLV_PCSK_SKI1_1	236	240	PF00082	0.373
DEG_Nend_UBRbox_3	1	3	PF02207	0.523
DOC_CYCLIN_RxL_1	16	25	PF00134	0.540
DOC_CYCLIN_RxL_1	179	190	PF00134	0.391
DOC_MAPK_gen_1	100	107	PF00069	0.458
DOC_MAPK_gen_1	139	145	PF00069	0.391
DOC_MAPK_gen_1	180	188	PF00069	0.336
DOC_MAPK_MEF2A_6	100	107	PF00069	0.487
DOC_MAPK_MEF2A_6	180	188	PF00069	0.290
DOC_MAPK_MEF2A_6	19	28	PF00069	0.538
DOC_PP4_FxxP_1	134	137	PF00568	0.361
DOC_USP7_MATH_1	38	42	PF00917	0.606
DOC_USP7_MATH_1	9	13	PF00917	0.459
LIG_14-3-3_CanoR_1	8	14	PF00244	0.630
LIG_Actin_WH2_2	84	102	PF00022	0.474
LIG_BRCT_BRCA1_1	117	121	PF00533	0.282
LIG_eIF4E_1	130	136	PF01652	0.361
LIG_FHA_1	23	29	PF00498	0.509
LIG_FHA_1	86	92	PF00498	0.405
LIG_FHA_2	111	117	PF00498	0.396
LIG_FHA_2	188	194	PF00498	0.308
LIG_LIR_Gen_1	211	222	PF02991	0.323
LIG_LIR_Nem_3	112	117	PF02991	0.385
LIG_LIR_Nem_3	211	217	PF02991	0.294
LIG_LIR_Nem_3	59	63	PF02991	0.464
LIG_PCNA_APIM_2	238	244	PF02747	0.591
LIG_Pex14_1	233	237	PF04695	0.490
LIG_Pex14_2	145	149	PF04695	0.290
LIG_REV1ctd_RIR_1	202	211	PF16727	0.336
LIG_SH2_CRK	47	51	PF00017	0.391
LIG_SH2_PTP2	214	217	PF00017	0.386
LIG_SH2_STAP1	177	181	PF00017	0.331
LIG_SH2_STAP1	47	51	PF00017	0.391
LIG_SH2_STAT5	203	206	PF00017	0.321
LIG_SH2_STAT5	214	217	PF00017	0.337
LIG_SH2_STAT5	237	240	PF00017	0.490
LIG_SH2_STAT5	241	244	PF00017	0.572
LIG_SH3_1	100	106	PF00018	0.462
LIG_SH3_3	100	106	PF00018	0.462
LIG_SH3_3	153	159	PF00018	0.316
LIG_SH3_3	165	171	PF00018	0.232
LIG_UBA3_1	135	140	PF00899	0.361
LIG_Vh1_VBS_1	13	31	PF01044	0.501
LIG_WRC_WIRS_1	111	116	PF05994	0.383
MOD_CK1_1	187	193	PF00069	0.361
MOD_CK2_1	188	194	PF00069	0.308
MOD_GlcNHglycan	149	152	PF01048	0.561
MOD_GlcNHglycan	210	213	PF01048	0.556
MOD_GSK3_1	184	191	PF00069	0.361
MOD_GSK3_1	4	11	PF00069	0.625
MOD_N-GLC_1	184	189	PF02516	0.561
MOD_NEK2_1	145	150	PF00069	0.361
MOD_NEK2_1	15	20	PF00069	0.576
MOD_NEK2_1	197	202	PF00069	0.363
MOD_NEK2_1	205	210	PF00069	0.340
MOD_NEK2_2	38	43	PF00069	0.681
MOD_PIKK_1	115	121	PF00454	0.361
MOD_Plk_1	115	121	PF00069	0.361
MOD_Plk_1	145	151	PF00069	0.361
MOD_Plk_1	184	190	PF00069	0.361
MOD_Plk_1	205	211	PF00069	0.339
MOD_Plk_4	228	234	PF00069	0.471
MOD_Plk_4	9	15	PF00069	0.562
MOD_SUMO_for_1	159	162	PF00179	0.391
TRG_DiLeu_BaEn_2	161	167	PF01217	0.361
TRG_ENDOCYTIC_2	214	217	PF00928	0.369
TRG_ENDOCYTIC_2	47	50	PF00928	0.335
TRG_ER_diArg_1	180	183	PF00400	0.367
TRG_ER_diArg_1	201	203	PF00400	0.361
TRG_ER_diLys_1	244	248	PF00400	0.606

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IAH0	Leptomonas seymouri	72%	100%
A0A1X0P077	Trypanosomatidae	45%	100%
A0A3Q8IJ75	Leishmania donovani	97%	100%
A0A422NKF6	Trypanosoma rangeli	46%	100%
A4HQ73	Leishmania braziliensis	85%	100%
A4IDX1	Leishmania infantum	97%	100%
E9ATZ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
V5BTC3	Trypanosoma cruzi	44%	100%