TRUD domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

pseudouridine synthase TruD, putative

Species:

Leishmania major

UniProt:

Q4Q0I6_LEIMA

TriTrypDb:

LmjF.36.5750 , LMJLV39_360069900 , LMJSD75_360069900

Length:

680

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

Q4Q0I6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q0I6

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006396	RNA processing	6	10
GO:0006399	tRNA metabolic process	7	10
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008033	tRNA processing	8	10
GO:0008152	metabolic process	1	11
GO:0009451	RNA modification	5	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0034470	ncRNA processing	7	10
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034660	ncRNA metabolic process	6	10
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0005488	binding	1	11
GO:0009982	pseudouridine synthase activity	4	11
GO:0016853	isomerase activity	2	11
GO:0016866	intramolecular transferase activity	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	226	230	PF00656	0.246
CLV_C14_Caspase3-7	596	600	PF00656	0.293
CLV_NRD_NRD_1	15	17	PF00675	0.543
CLV_NRD_NRD_1	202	204	PF00675	0.458
CLV_NRD_NRD_1	207	209	PF00675	0.485
CLV_NRD_NRD_1	28	30	PF00675	0.428
CLV_NRD_NRD_1	512	514	PF00675	0.398
CLV_PCSK_KEX2_1	100	102	PF00082	0.559
CLV_PCSK_KEX2_1	15	17	PF00082	0.435
CLV_PCSK_KEX2_1	167	169	PF00082	0.521
CLV_PCSK_KEX2_1	202	204	PF00082	0.458
CLV_PCSK_KEX2_1	206	208	PF00082	0.497
CLV_PCSK_KEX2_1	256	258	PF00082	0.246
CLV_PCSK_KEX2_1	27	29	PF00082	0.385
CLV_PCSK_KEX2_1	512	514	PF00082	0.362
CLV_PCSK_PC1ET2_1	100	102	PF00082	0.559
CLV_PCSK_PC1ET2_1	167	169	PF00082	0.521
CLV_PCSK_PC1ET2_1	256	258	PF00082	0.246
CLV_PCSK_PC1ET2_1	27	29	PF00082	0.460
CLV_PCSK_PC7_1	202	208	PF00082	0.469
CLV_PCSK_SKI1_1	168	172	PF00082	0.422
CLV_PCSK_SKI1_1	173	177	PF00082	0.392
CLV_PCSK_SKI1_1	257	261	PF00082	0.279
CLV_PCSK_SKI1_1	273	277	PF00082	0.246
CLV_PCSK_SKI1_1	311	315	PF00082	0.379
CLV_PCSK_SKI1_1	384	388	PF00082	0.249
CLV_PCSK_SKI1_1	424	428	PF00082	0.254
CLV_PCSK_SKI1_1	491	495	PF00082	0.250
CLV_PCSK_SKI1_1	512	516	PF00082	0.373
CLV_PCSK_SKI1_1	566	570	PF00082	0.387
DEG_APCC_DBOX_1	379	387	PF00400	0.379
DEG_APCC_DBOX_1	423	431	PF00400	0.246
DEG_APCC_DBOX_1	521	529	PF00400	0.370
DEG_APCC_KENBOX_2	224	228	PF00400	0.246
DEG_Nend_UBRbox_3	1	3	PF02207	0.532
DEG_SCF_FBW7_1	619	625	PF00400	0.512
DOC_CKS1_1	498	503	PF01111	0.245
DOC_CKS1_1	601	606	PF01111	0.473
DOC_CKS1_1	619	624	PF01111	0.584
DOC_MAPK_gen_1	254	261	PF00069	0.246
DOC_MAPK_gen_1	27	33	PF00069	0.420
DOC_MAPK_gen_1	378	387	PF00069	0.310
DOC_PP1_RVXF_1	244	251	PF00149	0.246
DOC_PP1_RVXF_1	489	495	PF00149	0.179
DOC_PP2B_LxvP_1	530	533	PF13499	0.246
DOC_PP4_FxxP_1	647	650	PF00568	0.293
DOC_PP4_FxxP_1	662	665	PF00568	0.313
DOC_USP7_MATH_1	119	123	PF00917	0.670
DOC_USP7_MATH_1	153	157	PF00917	0.552
DOC_USP7_MATH_1	158	162	PF00917	0.496
DOC_USP7_MATH_1	323	327	PF00917	0.419
DOC_USP7_MATH_1	47	51	PF00917	0.335
DOC_USP7_MATH_1	623	627	PF00917	0.622
DOC_USP7_MATH_1	86	90	PF00917	0.566
DOC_USP7_MATH_1	94	98	PF00917	0.766
DOC_WW_Pin1_4	497	502	PF00397	0.306
DOC_WW_Pin1_4	600	605	PF00397	0.495
DOC_WW_Pin1_4	618	623	PF00397	0.558
LIG_14-3-3_CanoR_1	15	24	PF00244	0.524
LIG_14-3-3_CanoR_1	257	262	PF00244	0.250
LIG_14-3-3_CanoR_1	267	272	PF00244	0.262
LIG_Actin_WH2_2	368	386	PF00022	0.359
LIG_APCC_ABBA_1	221	226	PF00400	0.246
LIG_BIR_III_2	496	500	PF00653	0.267
LIG_BIR_III_4	304	308	PF00653	0.324
LIG_BRCT_BRCA1_1	278	282	PF00533	0.324
LIG_EH1_1	453	461	PF00400	0.246
LIG_FHA_1	138	144	PF00498	0.503
LIG_FHA_1	360	366	PF00498	0.257
LIG_FHA_1	433	439	PF00498	0.209
LIG_FHA_1	509	515	PF00498	0.334
LIG_FHA_1	601	607	PF00498	0.433
LIG_FHA_1	619	625	PF00498	0.364
LIG_FHA_1	644	650	PF00498	0.361
LIG_FHA_1	654	660	PF00498	0.256
LIG_FHA_2	127	133	PF00498	0.522
LIG_FHA_2	16	22	PF00498	0.506
LIG_FHA_2	267	273	PF00498	0.379
LIG_FHA_2	333	339	PF00498	0.267
LIG_FHA_2	36	42	PF00498	0.370
LIG_FHA_2	485	491	PF00498	0.443
LIG_FHA_2	513	519	PF00498	0.277
LIG_FHA_2	54	60	PF00498	0.427
LIG_FHA_2	546	552	PF00498	0.379
LIG_Integrin_RGD_1	35	37	PF01839	0.434
LIG_LIR_Apic_2	599	604	PF02991	0.444
LIG_LIR_Apic_2	646	650	PF02991	0.293
LIG_LIR_Apic_2	661	665	PF02991	0.426
LIG_LIR_Gen_1	215	224	PF02991	0.363
LIG_LIR_Gen_1	458	469	PF02991	0.246
LIG_LIR_Gen_1	53	63	PF02991	0.313
LIG_LIR_Gen_1	550	559	PF02991	0.310
LIG_LIR_Nem_3	215	221	PF02991	0.372
LIG_LIR_Nem_3	415	421	PF02991	0.221
LIG_LIR_Nem_3	458	464	PF02991	0.310
LIG_LIR_Nem_3	53	58	PF02991	0.322
LIG_LIR_Nem_3	550	555	PF02991	0.338
LIG_LIR_Nem_3	580	584	PF02991	0.381
LIG_PCNA_PIPBox_1	397	406	PF02747	0.310
LIG_PCNA_yPIPBox_3	457	468	PF02747	0.359
LIG_Rb_pABgroove_1	398	406	PF01858	0.267
LIG_SH2_CRK	552	556	PF00017	0.379
LIG_SH2_CRK	581	585	PF00017	0.264
LIG_SH2_STAP1	409	413	PF00017	0.223
LIG_SH2_STAP1	552	556	PF00017	0.310
LIG_SH2_STAP1	654	658	PF00017	0.294
LIG_SH2_STAT5	354	357	PF00017	0.365
LIG_SH2_STAT5	418	421	PF00017	0.278
LIG_SH2_STAT5	437	440	PF00017	0.375
LIG_SH2_STAT5	452	455	PF00017	0.135
LIG_SH2_STAT5	55	58	PF00017	0.429
LIG_SH3_1	315	321	PF00018	0.286
LIG_SH3_3	130	136	PF00018	0.585
LIG_SH3_3	315	321	PF00018	0.299
LIG_SH3_3	525	531	PF00018	0.264
LIG_SH3_3	633	639	PF00018	0.503
LIG_SUMO_SIM_anti_2	567	573	PF11976	0.359
LIG_SUMO_SIM_par_1	399	405	PF11976	0.267
LIG_TRAF2_1	129	132	PF00917	0.510
LIG_TRAF2_1	19	22	PF00917	0.375
LIG_TRAF2_1	487	490	PF00917	0.267
LIG_TRFH_1	647	651	PF08558	0.303
LIG_WRC_WIRS_1	644	649	PF05994	0.399
MOD_CDK_SPxxK_3	600	607	PF00069	0.407
MOD_CK1_1	326	332	PF00069	0.295
MOD_CK1_1	440	446	PF00069	0.257
MOD_CK1_1	455	461	PF00069	0.215
MOD_CK1_1	467	473	PF00069	0.431
MOD_CK1_1	50	56	PF00069	0.323
MOD_CK1_1	503	509	PF00069	0.201
MOD_CK1_1	653	659	PF00069	0.338
MOD_CK1_1	80	86	PF00069	0.660
MOD_CK2_1	125	131	PF00069	0.517
MOD_CK2_1	15	21	PF00069	0.508
MOD_CK2_1	266	272	PF00069	0.379
MOD_CK2_1	484	490	PF00069	0.436
MOD_CK2_1	53	59	PF00069	0.433
MOD_CK2_1	605	611	PF00069	0.306
MOD_CK2_1	629	635	PF00069	0.652
MOD_CK2_1	674	680	PF00069	0.716
MOD_GlcNHglycan	120	124	PF01048	0.665
MOD_GlcNHglycan	625	628	PF01048	0.671
MOD_GlcNHglycan	631	634	PF01048	0.667
MOD_GlcNHglycan	79	82	PF01048	0.573
MOD_GlcNHglycan	84	87	PF01048	0.557
MOD_GlcNHglycan	88	91	PF01048	0.760
MOD_GSK3_1	360	367	PF00069	0.246
MOD_GSK3_1	428	435	PF00069	0.445
MOD_GSK3_1	440	447	PF00069	0.232
MOD_GSK3_1	463	470	PF00069	0.379
MOD_GSK3_1	508	515	PF00069	0.230
MOD_GSK3_1	541	548	PF00069	0.365
MOD_GSK3_1	618	625	PF00069	0.503
MOD_GSK3_1	650	657	PF00069	0.454
MOD_GSK3_1	82	89	PF00069	0.616
MOD_LATS_1	240	246	PF00433	0.379
MOD_NEK2_1	179	184	PF00069	0.417
MOD_NEK2_1	241	246	PF00069	0.265
MOD_NEK2_1	360	365	PF00069	0.325
MOD_NEK2_1	463	468	PF00069	0.293
MOD_NEK2_1	484	489	PF00069	0.310
MOD_NEK2_1	577	582	PF00069	0.379
MOD_NEK2_1	77	82	PF00069	0.541
MOD_NEK2_1	9	14	PF00069	0.385
MOD_NEK2_2	47	52	PF00069	0.336
MOD_PIKK_1	4	10	PF00454	0.412
MOD_PK_1	20	26	PF00069	0.399
MOD_PKA_1	15	21	PF00069	0.375
MOD_PKA_1	206	212	PF00069	0.624
MOD_PKA_1	512	518	PF00069	0.379
MOD_PKA_2	15	21	PF00069	0.513
MOD_PKA_2	206	212	PF00069	0.500
MOD_PKA_2	266	272	PF00069	0.338
MOD_PKA_2	467	473	PF00069	0.407
MOD_PKA_2	512	518	PF00069	0.305
MOD_PKB_1	206	214	PF00069	0.506
MOD_Plk_1	20	26	PF00069	0.424
MOD_Plk_1	326	332	PF00069	0.267
MOD_Plk_1	484	490	PF00069	0.310
MOD_Plk_1	589	595	PF00069	0.245
MOD_Plk_2-3	674	680	PF00069	0.592
MOD_Plk_4	190	196	PF00069	0.475
MOD_Plk_4	455	461	PF00069	0.259
MOD_Plk_4	50	56	PF00069	0.344
MOD_Plk_4	654	660	PF00069	0.338
MOD_Plk_4	9	15	PF00069	0.405
MOD_ProDKin_1	497	503	PF00069	0.306
MOD_ProDKin_1	600	606	PF00069	0.489
MOD_ProDKin_1	618	624	PF00069	0.560
TRG_DiLeu_BaEn_1	478	483	PF01217	0.379
TRG_DiLeu_BaEn_1	567	572	PF01217	0.359
TRG_DiLeu_BaEn_4	295	301	PF01217	0.310
TRG_DiLeu_BaLyEn_6	133	138	PF01217	0.496
TRG_ENDOCYTIC_2	218	221	PF00928	0.334
TRG_ENDOCYTIC_2	55	58	PF00928	0.296
TRG_ENDOCYTIC_2	552	555	PF00928	0.310
TRG_ENDOCYTIC_2	581	584	PF00928	0.246
TRG_ER_diArg_1	14	16	PF00400	0.552
TRG_ER_diArg_1	206	208	PF00400	0.515
TRG_ER_diArg_1	28	30	PF00400	0.419
TRG_ER_diArg_1	512	514	PF00400	0.356
TRG_NES_CRM1_1	373	388	PF08389	0.267
TRG_NLS_Bipartite_1	15	31	PF00514	0.369
TRG_Pf-PMV_PEXEL_1	384	388	PF00026	0.293
TRG_Pf-PMV_PEXEL_1	512	517	PF00026	0.407

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P912	Leptomonas seymouri	60%	95%
A0A1X0NMC1	Trypanosomatidae	38%	94%
A0A3Q8IJ87	Leishmania donovani	93%	100%
A0A3S7X658	Leishmania donovani	23%	95%
A0A422NSE9	Trypanosoma rangeli	42%	100%
A4HQ91	Leishmania braziliensis	75%	100%
A4I8G2	Leishmania infantum	23%	72%
A4IDY9	Leishmania infantum	93%	100%
D0A420	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	99%
E9AU09	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
O74343	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	100%
Q08647	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	100%
Q08DI8	Bos taurus	30%	100%
Q17426	Caenorhabditis elegans	27%	100%
Q1L8I0	Danio rerio	25%	100%
Q4Q4T4	Leishmania major	22%	96%
Q5V1E6	Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)	26%	100%
Q91VU7	Mus musculus	29%	100%
Q96PZ0	Homo sapiens	30%	100%
Q9VSK9	Drosophila melanogaster	27%	93%
V5BT95	Trypanosoma cruzi	42%	92%