Ubiquitin thioesterase OTU1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

OTU-like cysteine protease, putative

Species:

Leishmania major

UniProt:

Q4Q0F8_LEIMA

TriTrypDb:

LmjF.36.6020 , LMJLV39_360073000 , LMJSD75_360073000

Length:

384

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

Q4Q0F8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q0F8

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	12
GO:0006511	ubiquitin-dependent protein catabolic process	7	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0007165	signal transduction	2	2
GO:0008152	metabolic process	1	12
GO:0009056	catabolic process	2	12
GO:0009057	macromolecule catabolic process	4	12
GO:0009987	cellular process	1	12
GO:0010033	response to organic substance	3	12
GO:0010243	response to organonitrogen compound	4	12
GO:0010498	proteasomal protein catabolic process	5	12
GO:0016579	protein deubiquitination	6	12
GO:0019538	protein metabolic process	3	12
GO:0019941	modification-dependent protein catabolic process	6	12
GO:0030163	protein catabolic process	4	12
GO:0030433	ubiquitin-dependent ERAD pathway	6	12
GO:0030968	endoplasmic reticulum unfolded protein response	3	2
GO:0033554	cellular response to stress	3	12
GO:0034976	response to endoplasmic reticulum stress	4	12
GO:0036211	protein modification process	4	12
GO:0036503	ERAD pathway	5	12
GO:0042221	response to chemical	2	12
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043632	modification-dependent macromolecule catabolic process	5	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044248	cellular catabolic process	3	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0044265	obsolete cellular macromolecule catabolic process	4	12
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0050896	response to stimulus	1	12
GO:0051603	proteolysis involved in protein catabolic process	5	12
GO:0051716	cellular response to stimulus	2	12
GO:0065007	biological regulation	1	2
GO:0070646	protein modification by small protein removal	5	12
GO:0070647	protein modification by small protein conjugation or removal	5	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901565	organonitrogen compound catabolic process	4	12
GO:1901575	organic substance catabolic process	3	12
GO:1901698	response to nitrogen compound	3	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004843	cysteine-type deubiquitinase activity	5	12
GO:0008233	peptidase activity	3	12
GO:0008234	cysteine-type peptidase activity	4	12
GO:0016787	hydrolase activity	2	12
GO:0019783	ubiquitin-like protein peptidase activity	4	12
GO:0101005	deubiquitinase activity	5	12
GO:0140096	catalytic activity, acting on a protein	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	150	152	PF00675	0.417
CLV_NRD_NRD_1	221	223	PF00675	0.352
CLV_NRD_NRD_1	69	71	PF00675	0.514
CLV_PCSK_FUR_1	366	370	PF00082	0.415
CLV_PCSK_KEX2_1	220	222	PF00082	0.360
CLV_PCSK_KEX2_1	368	370	PF00082	0.401
CLV_PCSK_PC1ET2_1	368	370	PF00082	0.437
CLV_PCSK_SKI1_1	325	329	PF00082	0.419
DEG_SCF_FBW7_1	123	129	PF00400	0.458
DEG_SPOP_SBC_1	126	130	PF00917	0.495
DEG_SPOP_SBC_1	28	32	PF00917	0.389
DOC_CKS1_1	123	128	PF01111	0.446
DOC_MAPK_gen_1	151	157	PF00069	0.419
DOC_MAPK_gen_1	183	192	PF00069	0.423
DOC_SPAK_OSR1_1	65	69	PF12202	0.366
DOC_USP7_MATH_1	126	130	PF00917	0.482
DOC_USP7_MATH_1	225	229	PF00917	0.456
DOC_USP7_MATH_1	275	279	PF00917	0.335
DOC_WW_Pin1_4	122	127	PF00397	0.440
DOC_WW_Pin1_4	174	179	PF00397	0.542
LIG_14-3-3_CanoR_1	107	114	PF00244	0.396
LIG_14-3-3_CanoR_1	124	134	PF00244	0.433
LIG_14-3-3_CanoR_1	151	157	PF00244	0.420
LIG_14-3-3_CanoR_1	25	35	PF00244	0.385
LIG_14-3-3_CanoR_1	37	44	PF00244	0.421
LIG_14-3-3_CanoR_1	47	56	PF00244	0.377
LIG_14-3-3_CanoR_1	70	75	PF00244	0.402
LIG_BRCT_BRCA1_1	227	231	PF00533	0.275
LIG_FHA_1	7	13	PF00498	0.351
LIG_FHA_2	139	145	PF00498	0.486
LIG_LIR_Apic_2	206	211	PF02991	0.471
LIG_LIR_Gen_1	11	20	PF02991	0.323
LIG_LIR_Gen_1	91	101	PF02991	0.422
LIG_LIR_Nem_3	11	16	PF02991	0.320
LIG_LIR_Nem_3	91	97	PF02991	0.409
LIG_NRBOX	58	64	PF00104	0.434
LIG_PCNA_yPIPBox_3	250	258	PF02747	0.394
LIG_Pex14_2	17	21	PF04695	0.305
LIG_Pex14_2	60	64	PF04695	0.401
LIG_PTB_Apo_2	338	345	PF02174	0.359
LIG_PTB_Phospho_1	338	344	PF10480	0.376
LIG_SH2_CRK	335	339	PF00017	0.283
LIG_SH2_NCK_1	335	339	PF00017	0.266
LIG_SH2_NCK_1	344	348	PF00017	0.363
LIG_SH2_PTP2	208	211	PF00017	0.389
LIG_SH2_SRC	208	211	PF00017	0.417
LIG_SH2_STAP1	276	280	PF00017	0.392
LIG_SH2_STAP1	322	326	PF00017	0.316
LIG_SH2_STAP1	335	339	PF00017	0.354
LIG_SH2_STAT5	13	16	PF00017	0.412
LIG_SH2_STAT5	208	211	PF00017	0.417
LIG_SH2_STAT5	236	239	PF00017	0.313
LIG_SH2_STAT5	322	325	PF00017	0.290
LIG_SH2_STAT5	330	333	PF00017	0.284
LIG_SH3_1	207	213	PF00018	0.485
LIG_SH3_2	210	215	PF14604	0.486
LIG_SH3_3	207	213	PF00018	0.566
LIG_SH3_3	71	77	PF00018	0.400
LIG_SH3_3	87	93	PF00018	0.404
LIG_SUMO_SIM_anti_2	291	296	PF11976	0.276
LIG_SUMO_SIM_par_1	188	194	PF11976	0.374
LIG_TYR_ITIM	18	23	PF00017	0.308
LIG_TYR_ITSM	9	16	PF00017	0.298
LIG_UBA3_1	294	300	PF00899	0.411
LIG_WRC_WIRS_1	294	299	PF05994	0.292
MOD_CDK_SPK_2	122	127	PF00069	0.440
MOD_CK1_1	138	144	PF00069	0.473
MOD_CK1_1	150	156	PF00069	0.366
MOD_CK1_1	177	183	PF00069	0.458
MOD_CK1_1	278	284	PF00069	0.540
MOD_CK1_1	33	39	PF00069	0.400
MOD_CK1_1	79	85	PF00069	0.426
MOD_CK2_1	126	132	PF00069	0.437
MOD_CK2_1	138	144	PF00069	0.500
MOD_CK2_1	239	245	PF00069	0.493
MOD_CK2_1	343	349	PF00069	0.377
MOD_CK2_1	355	361	PF00069	0.381
MOD_GlcNHglycan	138	141	PF01048	0.491
MOD_GlcNHglycan	179	182	PF01048	0.433
MOD_GlcNHglycan	345	348	PF01048	0.414
MOD_GlcNHglycan	78	81	PF01048	0.458
MOD_GSK3_1	102	109	PF00069	0.470
MOD_GSK3_1	122	129	PF00069	0.467
MOD_GSK3_1	26	33	PF00069	0.413
MOD_GSK3_1	278	285	PF00069	0.485
MOD_GSK3_1	351	358	PF00069	0.439
MOD_GSK3_1	4	11	PF00069	0.366
MOD_GSK3_1	43	50	PF00069	0.447
MOD_GSK3_1	78	85	PF00069	0.466
MOD_N-GLC_1	136	141	PF02516	0.487
MOD_N-GLC_2	227	229	PF02516	0.286
MOD_NEK2_1	1	6	PF00069	0.428
MOD_NEK2_1	117	122	PF00069	0.476
MOD_NEK2_1	136	141	PF00069	0.483
MOD_NEK2_1	19	24	PF00069	0.364
MOD_NEK2_1	320	325	PF00069	0.371
MOD_NEK2_1	328	333	PF00069	0.428
MOD_NEK2_1	35	40	PF00069	0.437
MOD_NEK2_1	78	83	PF00069	0.394
MOD_OFUCOSY	223	229	PF10250	0.330
MOD_PIKK_1	278	284	PF00454	0.494
MOD_PK_1	70	76	PF00069	0.403
MOD_PKA_1	70	76	PF00069	0.403
MOD_PKA_2	106	112	PF00069	0.394
MOD_PKA_2	150	156	PF00069	0.412
MOD_PKA_2	26	32	PF00069	0.417
MOD_PKA_2	33	39	PF00069	0.410
MOD_PKA_2	99	105	PF00069	0.435
MOD_PKB_1	25	33	PF00069	0.379
MOD_Plk_1	225	231	PF00069	0.297
MOD_Plk_1	351	357	PF00069	0.440
MOD_Plk_4	275	281	PF00069	0.492
MOD_Plk_4	293	299	PF00069	0.292
MOD_Plk_4	351	357	PF00069	0.456
MOD_Plk_4	8	14	PF00069	0.306
MOD_ProDKin_1	122	128	PF00069	0.444
MOD_ProDKin_1	174	180	PF00069	0.543
MOD_SUMO_rev_2	194	199	PF00179	0.584
MOD_SUMO_rev_2	374	384	PF00179	0.448
TRG_DiLeu_BaEn_1	144	149	PF01217	0.556
TRG_DiLeu_BaEn_1	91	96	PF01217	0.366
TRG_DiLeu_BaEn_2	292	298	PF01217	0.417
TRG_ENDOCYTIC_2	13	16	PF00928	0.418
TRG_ENDOCYTIC_2	20	23	PF00928	0.349
TRG_ENDOCYTIC_2	335	338	PF00928	0.273
TRG_ER_diArg_1	121	124	PF00400	0.334
TRG_ER_diArg_1	159	162	PF00400	0.375
TRG_ER_diArg_1	220	222	PF00400	0.376
TRG_NES_CRM1_1	262	272	PF08389	0.324

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P901	Leptomonas seymouri	78%	100%
A0A0S4IPY5	Bodo saltans	48%	100%
A0A1X0NNB5	Trypanosomatidae	51%	100%
A0A3R7LXZ2	Trypanosoma rangeli	53%	100%
A0A3S7XC16	Leishmania donovani	81%	100%
A4HQC1	Leishmania braziliensis	75%	100%
A4IE15	Leishmania infantum	81%	100%
D0A3Y1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	100%
E9AU37	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
O13974	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	100%
Q55BI3	Dictyostelium discoideum	32%	100%
Q9VRJ9	Drosophila melanogaster	29%	100%
V5B3J2	Trypanosoma cruzi	54%	100%