Putative rab-like GTPase activating protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

disgorgin, putative

Species:

Leishmania major

UniProt:

Q4Q0F3_LEIMA

TriTrypDb:

LmjF.36.6070 , LMJLV39_360073500 , LMJSD75_360073500

Length:

498

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0000331	contractile vacuole	6	2
GO:0005773	vacuole	5	2
GO:0031410	cytoplasmic vesicle	6	2
GO:0031982	vesicle	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097708	intracellular vesicle	5	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q0F3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q0F3

Function

Biological processes
Term	Name	Level	Count
GO:0043085	positive regulation of catalytic activity	4	3
GO:0043087	regulation of GTPase activity	5	3
GO:0043547	positive regulation of GTPase activity	6	3
GO:0044093	positive regulation of molecular function	3	3
GO:0050790	regulation of catalytic activity	3	3
GO:0051336	regulation of hydrolase activity	4	3
GO:0051345	positive regulation of hydrolase activity	5	3
GO:0065007	biological regulation	1	3
GO:0065009	regulation of molecular function	2	3
GO:0090630	activation of GTPase activity	7	3

Molecular functions
Term	Name	Level	Count
GO:0005096	GTPase activator activity	4	3
GO:0008047	enzyme activator activity	3	3
GO:0030234	enzyme regulator activity	2	3
GO:0030695	GTPase regulator activity	4	3
GO:0060589	nucleoside-triphosphatase regulator activity	3	3
GO:0098772	molecular function regulator activity	1	3
GO:0140677	molecular function activator activity	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	460	464	PF00656	0.477
CLV_C14_Caspase3-7	8	12	PF00656	0.481
CLV_NRD_NRD_1	148	150	PF00675	0.612
CLV_NRD_NRD_1	200	202	PF00675	0.397
CLV_NRD_NRD_1	227	229	PF00675	0.407
CLV_NRD_NRD_1	469	471	PF00675	0.384
CLV_PCSK_KEX2_1	148	150	PF00082	0.691
CLV_PCSK_KEX2_1	200	202	PF00082	0.404
CLV_PCSK_KEX2_1	371	373	PF00082	0.299
CLV_PCSK_KEX2_1	383	385	PF00082	0.284
CLV_PCSK_KEX2_1	469	471	PF00082	0.403
CLV_PCSK_PC1ET2_1	371	373	PF00082	0.299
CLV_PCSK_PC1ET2_1	383	385	PF00082	0.284
CLV_PCSK_PC7_1	196	202	PF00082	0.380
CLV_PCSK_SKI1_1	140	144	PF00082	0.591
CLV_PCSK_SKI1_1	201	205	PF00082	0.406
CLV_PCSK_SKI1_1	302	306	PF00082	0.320
CLV_PCSK_SKI1_1	353	357	PF00082	0.383
CLV_PCSK_SKI1_1	411	415	PF00082	0.272
CLV_PCSK_SKI1_1	426	430	PF00082	0.342
CLV_Separin_Metazoa	466	470	PF03568	0.401
DEG_MDM2_SWIB_1	395	403	PF02201	0.385
DEG_MDM2_SWIB_1	421	428	PF02201	0.329
DOC_CKS1_1	141	146	PF01111	0.634
DOC_CYCLIN_RxL_1	407	418	PF00134	0.269
DOC_MAPK_gen_1	380	390	PF00069	0.329
DOC_MAPK_gen_1	469	476	PF00069	0.363
DOC_PP1_RVXF_1	199	206	PF00149	0.368
DOC_PP1_RVXF_1	234	241	PF00149	0.268
DOC_PP1_RVXF_1	424	430	PF00149	0.293
DOC_PP1_RVXF_1	471	477	PF00149	0.446
DOC_PP2B_LxvP_1	444	447	PF13499	0.361
DOC_PP4_FxxP_1	290	293	PF00568	0.266
DOC_USP7_UBL2_3	214	218	PF12436	0.411
DOC_USP7_UBL2_3	266	270	PF12436	0.385
DOC_WW_Pin1_4	140	145	PF00397	0.746
DOC_WW_Pin1_4	21	26	PF00397	0.692
DOC_WW_Pin1_4	48	53	PF00397	0.625
DOC_WW_Pin1_4	66	71	PF00397	0.801
DOC_WW_Pin1_4	85	90	PF00397	0.510
LIG_14-3-3_CanoR_1	66	70	PF00244	0.531
LIG_BRCT_BRCA1_1	396	400	PF00533	0.269
LIG_BRCT_BRCA1_1	67	71	PF00533	0.577
LIG_deltaCOP1_diTrp_1	202	212	PF00928	0.393
LIG_deltaCOP1_diTrp_1	423	429	PF00928	0.269
LIG_eIF4E_1	350	356	PF01652	0.376
LIG_eIF4E_1	368	374	PF01652	0.299
LIG_FHA_1	270	276	PF00498	0.333
LIG_FHA_1	284	290	PF00498	0.347
LIG_FHA_1	40	46	PF00498	0.550
LIG_FHA_1	400	406	PF00498	0.288
LIG_FHA_1	476	482	PF00498	0.330
LIG_FHA_2	130	136	PF00498	0.722
LIG_GBD_Chelix_1	324	332	PF00786	0.269
LIG_Integrin_isoDGR_2	28	30	PF01839	0.478
LIG_LIR_Gen_1	202	210	PF02991	0.417
LIG_LIR_Gen_1	246	254	PF02991	0.330
LIG_LIR_Gen_1	35	43	PF02991	0.563
LIG_LIR_Gen_1	397	408	PF02991	0.278
LIG_LIR_Gen_1	463	471	PF02991	0.527
LIG_LIR_Gen_1	486	495	PF02991	0.471
LIG_LIR_LC3C_4	51	56	PF02991	0.500
LIG_LIR_Nem_3	172	177	PF02991	0.445
LIG_LIR_Nem_3	233	238	PF02991	0.391
LIG_LIR_Nem_3	246	250	PF02991	0.315
LIG_LIR_Nem_3	335	341	PF02991	0.308
LIG_LIR_Nem_3	35	39	PF02991	0.571
LIG_LIR_Nem_3	397	403	PF02991	0.316
LIG_LIR_Nem_3	422	428	PF02991	0.280
LIG_LIR_Nem_3	463	468	PF02991	0.540
LIG_LIR_Nem_3	486	492	PF02991	0.431
LIG_OCRL_FandH_1	337	349	PF00620	0.293
LIG_PALB2_WD40_1	395	403	PF16756	0.269
LIG_PAM2_1	390	402	PF00658	0.316
LIG_Pex14_1	417	421	PF04695	0.320
LIG_Pex14_1	425	429	PF04695	0.298
LIG_Pex14_2	356	360	PF04695	0.353
LIG_Pex14_2	395	399	PF04695	0.317
LIG_Pex14_2	400	404	PF04695	0.302
LIG_Pex14_2	421	425	PF04695	0.351
LIG_PTB_Apo_2	344	351	PF02174	0.421
LIG_PTB_Phospho_1	344	350	PF10480	0.421
LIG_SH2_PTP2	259	262	PF00017	0.304
LIG_SH2_STAT5	189	192	PF00017	0.481
LIG_SH2_STAT5	259	262	PF00017	0.304
LIG_SH2_STAT5	317	320	PF00017	0.309
LIG_SH3_3	108	114	PF00018	0.580
LIG_SH3_3	355	361	PF00018	0.295
LIG_SH3_3	46	52	PF00018	0.623
LIG_SUMO_SIM_anti_2	431	437	PF11976	0.339
LIG_SUMO_SIM_anti_2	50	57	PF11976	0.585
LIG_TRAF2_1	132	135	PF00917	0.609
LIG_TRAF2_1	333	336	PF00917	0.304
LIG_TRAF2_1	58	61	PF00917	0.753
LIG_UBA3_1	377	383	PF00899	0.335
LIG_UBA3_1	452	456	PF00899	0.413
LIG_WRC_WIRS_1	244	249	PF05994	0.385
MOD_CDC14_SPxK_1	24	27	PF00782	0.483
MOD_CDK_SPxK_1	21	27	PF00069	0.550
MOD_CK1_1	88	94	PF00069	0.679
MOD_CK2_1	127	133	PF00069	0.720
MOD_CK2_1	154	160	PF00069	0.592
MOD_CK2_1	32	38	PF00069	0.607
MOD_DYRK1A_RPxSP_1	66	70	PF00069	0.554
MOD_GlcNHglycan	156	159	PF01048	0.726
MOD_GSK3_1	125	132	PF00069	0.638
MOD_GSK3_1	136	143	PF00069	0.638
MOD_GSK3_1	28	35	PF00069	0.541
MOD_GSK3_1	390	397	PF00069	0.251
MOD_GSK3_1	438	445	PF00069	0.368
MOD_GSK3_1	9	16	PF00069	0.613
MOD_N-GLC_1	154	159	PF02516	0.729
MOD_N-GLC_1	212	217	PF02516	0.501
MOD_NEK2_1	115	120	PF00069	0.677
MOD_NEK2_1	13	18	PF00069	0.728
MOD_NEK2_1	243	248	PF00069	0.309
MOD_NEK2_1	280	285	PF00069	0.316
MOD_NEK2_1	394	399	PF00069	0.269
MOD_NEK2_1	448	453	PF00069	0.545
MOD_NEK2_1	64	69	PF00069	0.519
MOD_NEK2_2	399	404	PF00069	0.309
MOD_PIKK_1	115	121	PF00454	0.668
MOD_PIKK_1	13	19	PF00454	0.670
MOD_PIKK_1	327	333	PF00454	0.269
MOD_PIKK_1	394	400	PF00454	0.269
MOD_PKA_1	148	154	PF00069	0.521
MOD_PKA_2	148	154	PF00069	0.710
MOD_PKA_2	379	385	PF00069	0.368
MOD_PKA_2	65	71	PF00069	0.719
MOD_Plk_1	154	160	PF00069	0.606
MOD_Plk_1	334	340	PF00069	0.269
MOD_Plk_1	438	444	PF00069	0.364
MOD_Plk_1	448	454	PF00069	0.427
MOD_Plk_2-3	129	135	PF00069	0.703
MOD_Plk_4	296	302	PF00069	0.270
MOD_Plk_4	334	340	PF00069	0.292
MOD_Plk_4	390	396	PF00069	0.307
MOD_Plk_4	399	405	PF00069	0.331
MOD_Plk_4	448	454	PF00069	0.328
MOD_ProDKin_1	140	146	PF00069	0.745
MOD_ProDKin_1	21	27	PF00069	0.692
MOD_ProDKin_1	48	54	PF00069	0.628
MOD_ProDKin_1	66	72	PF00069	0.799
MOD_ProDKin_1	85	91	PF00069	0.511
MOD_SUMO_for_1	436	439	PF00179	0.309
MOD_SUMO_rev_2	156	166	PF00179	0.488
MOD_SUMO_rev_2	180	190	PF00179	0.537
TRG_DiLeu_BaEn_1	439	444	PF01217	0.309
TRG_DiLeu_BaEn_1	490	495	PF01217	0.376
TRG_DiLeu_BaEn_4	439	445	PF01217	0.298
TRG_DiLeu_BaLyEn_6	358	363	PF01217	0.368
TRG_DiLeu_BaLyEn_6	369	374	PF01217	0.300
TRG_DiLeu_BaLyEn_6	49	54	PF01217	0.553
TRG_ENDOCYTIC_2	259	262	PF00928	0.329
TRG_ENDOCYTIC_2	36	39	PF00928	0.570
TRG_ENDOCYTIC_2	465	468	PF00928	0.326
TRG_ER_diArg_1	199	201	PF00400	0.371
TRG_ER_diArg_1	468	470	PF00400	0.391
TRG_ER_diArg_1	94	97	PF00400	0.574
TRG_Pf-PMV_PEXEL_1	383	387	PF00026	0.385
TRG_Pf-PMV_PEXEL_1	96	101	PF00026	0.511

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I3T4	Leptomonas seymouri	36%	100%
A0A0N1PDX9	Leptomonas seymouri	76%	91%
A0A0S4II34	Bodo saltans	54%	100%
A0A1X0NLQ9	Trypanosomatidae	64%	100%
A0A3S7XBZ8	Leishmania donovani	36%	100%
A0A3S7XC15	Leishmania donovani	92%	100%
A4HQC6	Leishmania braziliensis	79%	100%
A4HQC7	Leishmania braziliensis	37%	100%
A4IE19	Leishmania infantum	92%	100%
A4IE20	Leishmania infantum	36%	100%
E9AU42	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	89%
E9AU43	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
P58802	Mus musculus	33%	100%
Q4Q0F2	Leishmania major	42%	100%
Q9P2M4	Homo sapiens	27%	72%
V5BT66	Trypanosoma cruzi	63%	100%