EF-hand domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Protein of unknown function (DUF2453), putative

Species:

Leishmania major

UniProt:

Q4Q071_LEIMA

TriTrypDb:

LmjF.36.6840 , LMJLV39_360083600 , LMJSD75_360083400

Length:

350

Annotations

LeishMANIAdb annotations

These Kinetoplastid proteins share considerable homology with the animal TMEM65 proteins at their membrane domains, with a unique EF-hand at the N-terminus.. While the animal orthologs have a mitochondrial transit signal, it seems to be absent from the Kinetoplastid proteins. The latter have an alanine-rich segment of unclear function.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	2
GO:0016020	membrane	2	12
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

Q4Q071

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q071

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	11
GO:0005509	calcium ion binding	5	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	186	190	PF00656	0.244
CLV_C14_Caspase3-7	341	345	PF00656	0.371
CLV_NRD_NRD_1	115	117	PF00675	0.360
CLV_NRD_NRD_1	244	246	PF00675	0.258
CLV_NRD_NRD_1	68	70	PF00675	0.329
CLV_PCSK_KEX2_1	114	116	PF00082	0.355
CLV_PCSK_KEX2_1	122	124	PF00082	0.412
CLV_PCSK_KEX2_1	244	246	PF00082	0.256
CLV_PCSK_KEX2_1	67	69	PF00082	0.329
CLV_PCSK_PC1ET2_1	114	116	PF00082	0.406
CLV_PCSK_PC1ET2_1	122	124	PF00082	0.460
CLV_PCSK_SKI1_1	122	126	PF00082	0.531
CLV_PCSK_SKI1_1	128	132	PF00082	0.579
CLV_PCSK_SKI1_1	244	248	PF00082	0.260
CLV_PCSK_SKI1_1	346	350	PF00082	0.641
DEG_SCF_FBW7_1	231	237	PF00400	0.525
DEG_SPOP_SBC_1	30	34	PF00917	0.779
DOC_CDC14_PxL_1	264	272	PF14671	0.360
DOC_CKS1_1	231	236	PF01111	0.498
DOC_CYCLIN_RxL_1	303	314	PF00134	0.356
DOC_PP1_RVXF_1	327	333	PF00149	0.376
DOC_USP7_MATH_1	148	152	PF00917	0.697
DOC_USP7_MATH_1	30	34	PF00917	0.672
DOC_USP7_UBL2_3	224	228	PF12436	0.447
DOC_WW_Pin1_4	142	147	PF00397	0.739
DOC_WW_Pin1_4	230	235	PF00397	0.479
DOC_WW_Pin1_4	98	103	PF00397	0.571
LIG_BIR_II_1	1	5	PF00653	0.650
LIG_BRCT_BRCA1_1	190	194	PF00533	0.335
LIG_Clathr_ClatBox_1	269	273	PF01394	0.325
LIG_FHA_1	178	184	PF00498	0.275
LIG_FHA_1	20	26	PF00498	0.720
LIG_FHA_1	231	237	PF00498	0.498
LIG_FHA_1	253	259	PF00498	0.275
LIG_FHA_2	129	135	PF00498	0.726
LIG_FHA_2	35	41	PF00498	0.590
LIG_LIR_Gen_1	280	290	PF02991	0.391
LIG_LIR_Gen_1	293	301	PF02991	0.312
LIG_LIR_Gen_1	333	342	PF02991	0.371
LIG_LIR_Nem_3	280	285	PF02991	0.395
LIG_LIR_Nem_3	293	298	PF02991	0.315
LIG_LIR_Nem_3	300	304	PF02991	0.192
LIG_LIR_Nem_3	333	338	PF02991	0.326
LIG_Pex14_2	105	109	PF04695	0.563
LIG_PTB_Apo_2	289	296	PF02174	0.384
LIG_SH2_GRB2like	195	198	PF00017	0.325
LIG_SH2_STAT5	195	198	PF00017	0.300
LIG_SH2_STAT5	282	285	PF00017	0.395
LIG_SH3_3	293	299	PF00018	0.338
LIG_SUMO_SIM_par_1	160	165	PF11976	0.360
LIG_TRAF2_1	85	88	PF00917	0.662
MOD_CDK_SPK_2	98	103	PF00069	0.572
MOD_CK1_1	135	141	PF00069	0.731
MOD_CK1_1	29	35	PF00069	0.708
MOD_CK2_1	128	134	PF00069	0.766
MOD_CK2_1	34	40	PF00069	0.611
MOD_GlcNHglycan	110	113	PF01048	0.373
MOD_GlcNHglycan	12	15	PF01048	0.365
MOD_GlcNHglycan	134	137	PF01048	0.585
MOD_GlcNHglycan	202	205	PF01048	0.303
MOD_GlcNHglycan	210	213	PF01048	0.239
MOD_GlcNHglycan	26	29	PF01048	0.561
MOD_GlcNHglycan	292	295	PF01048	0.554
MOD_GlcNHglycan	83	86	PF01048	0.418
MOD_GSK3_1	128	135	PF00069	0.703
MOD_GSK3_1	196	203	PF00069	0.360
MOD_GSK3_1	230	237	PF00069	0.477
MOD_GSK3_1	26	33	PF00069	0.687
MOD_GSK3_1	273	280	PF00069	0.391
MOD_N-GLC_1	177	182	PF02516	0.495
MOD_N-GLC_1	196	201	PF02516	0.169
MOD_N-GLC_1	290	295	PF02516	0.595
MOD_N-GLC_2	208	210	PF02516	0.411
MOD_NEK2_1	1	6	PF00069	0.731
MOD_NEK2_1	176	181	PF00069	0.303
MOD_NEK2_1	196	201	PF00069	0.145
MOD_NEK2_1	252	257	PF00069	0.324
MOD_NEK2_1	70	75	PF00069	0.647
MOD_PIKK_1	333	339	PF00454	0.366
MOD_PKA_1	122	128	PF00069	0.651
MOD_PKA_2	122	128	PF00069	0.713
MOD_Plk_1	176	182	PF00069	0.246
MOD_Plk_1	196	202	PF00069	0.145
MOD_Plk_4	177	183	PF00069	0.256
MOD_Plk_4	196	202	PF00069	0.169
MOD_Plk_4	31	37	PF00069	0.758
MOD_ProDKin_1	142	148	PF00069	0.739
MOD_ProDKin_1	230	236	PF00069	0.479
MOD_ProDKin_1	98	104	PF00069	0.564
MOD_SUMO_rev_2	341	348	PF00179	0.468
TRG_DiLeu_BaLyEn_6	265	270	PF01217	0.266
TRG_ENDOCYTIC_2	282	285	PF00928	0.383
TRG_ER_diArg_1	244	246	PF00400	0.453
TRG_ER_diArg_1	67	69	PF00400	0.528
TRG_NLS_MonoExtC_3	120	126	PF00514	0.697
TRG_Pf-PMV_PEXEL_1	303	307	PF00026	0.568
TRG_Pf-PMV_PEXEL_1	318	322	PF00026	0.559
TRG_Pf-PMV_PEXEL_1	5	9	PF00026	0.472
TRG_Pf-PMV_PEXEL_1	53	57	PF00026	0.432

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P806	Leptomonas seymouri	80%	99%
A0A0S4J8V3	Bodo saltans	34%	100%
A0A0S4JY67	Bodo saltans	56%	100%
A0A1X0NL71	Trypanosomatidae	55%	100%
A0A3S7XCB7	Leishmania donovani	95%	100%
A4HQK4	Leishmania braziliensis	85%	100%
A4ICC4	Leishmania infantum	96%	100%
E9AUB9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
V5BGX0	Trypanosoma cruzi	35%	84%
V5BHN9	Trypanosoma cruzi	54%	100%