Surface antigen 2

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Surface antigen 2

Gene product:

surface antigen protein 2 precursor

Species:

Leishmania major

UniProt:

Q25331_LEIMA

TriTrypDb:

LmjF.12.0780 , LmjF.12.0980 , LMJLV39_120012900 , LMJSD75_120012600

Length:

385

Annotations

LeishMANIAdb annotations

Publication identifier(s): 8702946
A surface coat protein involved in immune evasion in Leishmaniids. Extremely fast evolving, almost completely disordered mucin-like protein. . Localization: Cell surface (experimental)

LeishMANIAdb interaction annotations

by homology
Contact email: handman@wehi.edu.au
Publication title: A Leucine-Rich Repeat Motif of Leishmania Parasite Surface Antigen 2 Binds to Macrophages through the Complement Receptor 3
Publication 1st author(s): Kedzierski
Publication Identifier(s): 15067069
Host organism: -1
Interaction detection method(s): fluorescence activated cell sorting
Interaction type: physical association
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: P11215
ID(s) interactor B: E9AGG4
Taxid interactor A: Homo sapiens
Taxid interactor B: Leishmania infantum
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: unspecified role

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 120

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 48, no: 8
NetGPI	no	yes: 0, no: 56

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	57
GO:0016020	membrane	2	15
GO:0042995	cell projection	2	57
GO:0043226	organelle	2	57
GO:0043227	membrane-bounded organelle	3	57
GO:0110165	cellular anatomical entity	1	57
GO:0120025	plasma membrane bounded cell projection	3	57
GO:0005886	plasma membrane	3	3

Expansion

Sequence features

Q25331

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q25331

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0004672	protein kinase activity	3	1
GO:0004674	protein serine/threonine kinase activity	4	1
GO:0016301	kinase activity	4	1
GO:0016740	transferase activity	2	1
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	1
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	6	8	PF00675	0.694
CLV_PCSK_KEX2_1	6	8	PF00082	0.694
CLV_PCSK_SKI1_1	7	11	PF00082	0.708
DEG_SCF_FBW7_1	272	278	PF00400	0.472
DEG_SCF_FBW7_2	313	318	PF00400	0.471
DEG_SPOP_SBC_1	275	279	PF00917	0.476
DEG_SPOP_SBC_1	281	285	PF00917	0.475
DEG_SPOP_SBC_1	293	297	PF00917	0.474
DOC_CKS1_1	272	277	PF01111	0.472
DOC_CYCLIN_RxL_1	105	116	PF00134	0.304
DOC_CYCLIN_RxL_1	182	193	PF00134	0.301
DOC_CYCLIN_RxL_1	3	13	PF00134	0.512
DOC_MAPK_MEF2A_6	229	236	PF00069	0.382
DOC_PP1_RVXF_1	108	115	PF00149	0.308
DOC_USP7_MATH_1	209	213	PF00917	0.325
DOC_USP7_MATH_1	361	365	PF00917	0.281
DOC_USP7_MATH_2	151	157	PF00917	0.307
DOC_USP7_UBL2_3	350	354	PF12436	0.330
DOC_USP7_UBL2_3	59	63	PF12436	0.319
DOC_WW_Pin1_4	262	267	PF00397	0.489
DOC_WW_Pin1_4	271	276	PF00397	0.470
DOC_WW_Pin1_4	305	310	PF00397	0.503
DOC_WW_Pin1_4	311	316	PF00397	0.480
DOC_WW_Pin1_4	85	90	PF00397	0.312
LIG_14-3-3_CanoR_1	276	281	PF00244	0.474
LIG_14-3-3_CanoR_1	43	48	PF00244	0.352
LIG_Actin_WH2_2	153	168	PF00022	0.290
LIG_BIR_II_1	1	5	PF00653	0.601
LIG_deltaCOP1_diTrp_1	62	69	PF00928	0.353
LIG_EVH1_1	271	275	PF00568	0.471
LIG_FHA_1	263	269	PF00498	0.468
LIG_FHA_1	277	283	PF00498	0.462
LIG_FHA_1	42	48	PF00498	0.378
LIG_FHA_2	131	137	PF00498	0.460
LIG_FHA_2	275	281	PF00498	0.472
LIG_FHA_2	283	289	PF00498	0.473
LIG_FHA_2	293	299	PF00498	0.472
LIG_FHA_2	317	323	PF00498	0.412
LIG_FHA_2	92	98	PF00498	0.394
LIG_LIR_Gen_1	113	121	PF02991	0.339
LIG_LIR_Gen_1	128	135	PF02991	0.342
LIG_LIR_Gen_1	200	209	PF02991	0.367
LIG_LIR_Gen_1	71	80	PF02991	0.336
LIG_LIR_Nem_3	103	107	PF02991	0.367
LIG_LIR_Nem_3	200	205	PF02991	0.336
LIG_LIR_Nem_3	71	75	PF02991	0.358
LIG_PDZ_Class_2	380	385	PF00595	0.292
LIG_SH2_STAT5	344	347	PF00017	0.333
LIG_SH2_STAT5	83	86	PF00017	0.335
LIG_SH3_3	269	275	PF00018	0.472
LIG_SH3_3	287	293	PF00018	0.466
LIG_SH3_3	297	303	PF00018	0.491
LIG_SH3_3	93	99	PF00018	0.404
LIG_Sin3_3	369	376	PF02671	0.265
LIG_SUMO_SIM_anti_2	158	164	PF11976	0.299
LIG_SUMO_SIM_anti_2	46	51	PF11976	0.327
LIG_SUMO_SIM_par_1	232	238	PF11976	0.348
LIG_TYR_ITIM	102	107	PF00017	0.374
MOD_CDK_SPK_2	271	276	PF00069	0.473
MOD_CK1_1	128	134	PF00069	0.374
MOD_CK1_1	156	162	PF00069	0.416
MOD_CK1_1	179	185	PF00069	0.358
MOD_CK1_1	197	203	PF00069	0.315
MOD_CK1_1	259	265	PF00069	0.475
MOD_CK1_1	85	91	PF00069	0.346
MOD_CK2_1	130	136	PF00069	0.341
MOD_CK2_1	274	280	PF00069	0.476
MOD_CK2_1	282	288	PF00069	0.475
MOD_CK2_1	292	298	PF00069	0.469
MOD_CK2_1	316	322	PF00069	0.438
MOD_CK2_1	91	97	PF00069	0.404
MOD_GlcNHglycan	121	124	PF01048	0.617
MOD_GlcNHglycan	146	149	PF01048	0.555
MOD_GlcNHglycan	170	173	PF01048	0.666
MOD_GlcNHglycan	178	181	PF01048	0.565
MOD_GlcNHglycan	193	197	PF01048	0.576
MOD_GlcNHglycan	202	205	PF01048	0.524
MOD_GlcNHglycan	25	28	PF01048	0.659
MOD_GlcNHglycan	258	261	PF01048	0.655
MOD_GlcNHglycan	332	335	PF01048	0.563
MOD_GlcNHglycan	364	367	PF01048	0.296
MOD_GlcNHglycan	84	87	PF01048	0.541
MOD_GSK3_1	121	128	PF00069	0.462
MOD_GSK3_1	130	137	PF00069	0.372
MOD_GSK3_1	142	149	PF00069	0.443
MOD_GSK3_1	179	186	PF00069	0.484
MOD_GSK3_1	194	201	PF00069	0.307
MOD_GSK3_1	249	256	PF00069	0.625
MOD_GSK3_1	258	265	PF00069	0.501
MOD_GSK3_1	271	278	PF00069	0.471
MOD_GSK3_1	280	287	PF00069	0.473
MOD_GSK3_1	301	308	PF00069	0.497
MOD_GSK3_1	32	39	PF00069	0.532
MOD_GSK3_1	344	351	PF00069	0.358
MOD_GSK3_1	87	94	PF00069	0.348
MOD_N-GLC_1	266	271	PF02516	0.670
MOD_NEK2_1	10	15	PF00069	0.523
MOD_NEK2_1	121	126	PF00069	0.429
MOD_NEK2_1	165	170	PF00069	0.416
MOD_NEK2_1	194	199	PF00069	0.478
MOD_NEK2_1	207	212	PF00069	0.305
MOD_NEK2_1	21	26	PF00069	0.510
MOD_NEK2_1	282	287	PF00069	0.478
MOD_NEK2_2	209	214	PF00069	0.292
MOD_PK_1	142	148	PF00069	0.314
MOD_PKA_2	165	171	PF00069	0.303
MOD_PKA_2	275	281	PF00069	0.472
MOD_Plk_1	348	354	PF00069	0.364
MOD_Plk_2-3	136	142	PF00069	0.291
MOD_Plk_2-3	68	74	PF00069	0.323
MOD_Plk_4	158	164	PF00069	0.299
MOD_ProDKin_1	262	268	PF00069	0.491
MOD_ProDKin_1	271	277	PF00069	0.470
MOD_ProDKin_1	305	311	PF00069	0.502
MOD_ProDKin_1	85	91	PF00069	0.309
MOD_SUMO_rev_2	224	230	PF00179	0.336
MOD_SUMO_rev_2	342	351	PF00179	0.342
TRG_ENDOCYTIC_2	104	107	PF00928	0.402
TRG_ER_diArg_1	5	7	PF00400	0.586
TRG_Pf-PMV_PEXEL_1	185	190	PF00026	0.501

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZ17	Leptomonas seymouri	32%	100%
A0A0S4JEJ4	Bodo saltans	24%	100%
A0A3Q8I9A6	Leishmania donovani	55%	100%
A0A3Q8I9D0	Leishmania donovani	56%	100%
A0A3Q8IFC2	Leishmania donovani	40%	100%
A0A3Q8IIJ9	Leishmania donovani	31%	100%
A0A3S5H6D6	Leishmania donovani	32%	100%
A0A3S5H6L9	Leishmania donovani	57%	66%
A0A3S7WP69	Leishmania donovani	21%	100%
A0A3S7WPB2	Leishmania donovani	30%	100%
A0A3S7X4J4	Leishmania donovani	31%	100%
A4H4D2	Leishmania braziliensis	24%	100%
A4H4G6	Leishmania braziliensis	29%	100%
A4H5P0	Leishmania braziliensis	32%	100%
A4HBX3	Leishmania braziliensis	31%	85%
A4HJC8	Leishmania braziliensis	30%	100%
A4HJX1	Leishmania braziliensis	37%	100%
A4HSL2	Leishmania infantum	21%	100%
A4HTX9	Leishmania infantum	32%	100%
A4HVB0	Leishmania infantum	50%	96%
A4I6S4	Leishmania infantum	31%	100%
D1GJ51	Leishmania infantum	49%	83%
E8NHG5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	87%
E8NHP8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9ACQ0	Leishmania major	30%	100%
E9AG65	Leishmania infantum	30%	100%
E9AGG5	Leishmania infantum	56%	92%
E9AGG9	Leishmania infantum	56%	71%
E9AGH0	Leishmania infantum	55%	100%
E9AKJ6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	22%	100%
E9AKM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9AMQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9AP02	Leishmania mexicana (strain MHOM/GT/2001/U1103)	55%	100%
E9AP03	Leishmania mexicana (strain MHOM/GT/2001/U1103)	52%	98%
E9AP05	Leishmania mexicana (strain MHOM/GT/2001/U1103)	49%	75%
E9AP07	Leishmania mexicana (strain MHOM/GT/2001/U1103)	57%	100%
E9AP08	Leishmania mexicana (strain MHOM/GT/2001/U1103)	54%	92%
E9B1U3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	85%
E9B1U4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9B1U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	92%
E9B1U6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
P21978	Leishmania amazonensis	52%	81%
Q4Q6B6	Leishmania major	30%	100%
Q4Q6B7	Leishmania major	30%	100%
Q4QC79	Leishmania major	29%	84%
Q4QGI0	Leishmania major	60%	76%
Q4QGI2	Leishmania major	46%	69%
Q4QGI4	Leishmania major	47%	72%
Q4QGI6	Leishmania major	72%	70%
Q4QGJ4	Leishmania major	99%	100%
Q4QGJ7	Leishmania major	94%	100%
Q4QGK2	Leishmania major	52%	69%
Q4QGK6	Leishmania major	100%	100%
Q4QGL4	Leishmania major	63%	97%
Q4QHW6	Leishmania major	33%	100%
Q4QJB2	Leishmania major	21%	100%