Putative RNA-binding protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Double RNA binding domain protein 3

Species:

Leishmania major

UniProt:

O97003_LEIMA

TriTrypDb:

LmjF.04.1170 , LMJLV39_040017400 , LMJSD75_040017700

Length:

311

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005654	nucleoplasm	2	2
GO:0005737	cytoplasm	2	2
GO:0005829	cytosol	2	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

O97003

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: O97003

Function

Biological processes
Term	Name	Level	Count
GO:0009892	negative regulation of metabolic process	4	2
GO:0009893	positive regulation of metabolic process	4	2
GO:0009894	regulation of catabolic process	4	2
GO:0009895	negative regulation of catabolic process	5	2
GO:0010468	regulation of gene expression	5	2
GO:0010604	positive regulation of macromolecule metabolic process	5	2
GO:0010605	negative regulation of macromolecule metabolic process	5	2
GO:0010608	post-transcriptional regulation of gene expression	6	2
GO:0010628	positive regulation of gene expression	6	2
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	2
GO:0019222	regulation of metabolic process	3	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031324	negative regulation of cellular metabolic process	5	2
GO:0031329	regulation of cellular catabolic process	5	2
GO:0031330	negative regulation of cellular catabolic process	6	2
GO:0043487	regulation of RNA stability	3	2
GO:0043488	regulation of mRNA stability	4	2
GO:0043489	RNA stabilization	4	2
GO:0045934	negative regulation of nucleobase-containing compound metabolic process	6	2
GO:0048255	mRNA stabilization	5	2
GO:0048518	positive regulation of biological process	3	2
GO:0048519	negative regulation of biological process	3	2
GO:0048523	negative regulation of cellular process	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051172	negative regulation of nitrogen compound metabolic process	5	2
GO:0051252	regulation of RNA metabolic process	5	2
GO:0051253	negative regulation of RNA metabolic process	6	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0061013	regulation of mRNA catabolic process	6	2
GO:0065007	biological regulation	1	2
GO:0065008	regulation of biological quality	2	2
GO:0080090	regulation of primary metabolic process	4	2
GO:1902369	negative regulation of RNA catabolic process	7	2
GO:1902373	negative regulation of mRNA catabolic process	7	2
GO:1903311	regulation of mRNA metabolic process	6	2
GO:1903312	negative regulation of mRNA metabolic process	7	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003729	mRNA binding	5	2
GO:0003730	mRNA 3'-UTR binding	6	2
GO:0005488	binding	1	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	151	157	PF00089	0.569
CLV_NRD_NRD_1	149	151	PF00675	0.457
CLV_PCSK_KEX2_1	149	151	PF00082	0.457
CLV_PCSK_SKI1_1	169	173	PF00082	0.431
CLV_PCSK_SKI1_1	238	242	PF00082	0.334
CLV_PCSK_SKI1_1	88	92	PF00082	0.301
DEG_APCC_DBOX_1	168	176	PF00400	0.554
DEG_Nend_Nbox_1	1	3	PF02207	0.637
DEG_SCF_FBW7_1	90	95	PF00400	0.509
DEG_SPOP_SBC_1	298	302	PF00917	0.721
DOC_CYCLIN_RxL_1	166	174	PF00134	0.449
DOC_MAPK_gen_1	149	155	PF00069	0.569
DOC_PP2B_LxvP_1	25	28	PF13499	0.702
DOC_PP4_FxxP_1	138	141	PF00568	0.568
DOC_PP4_MxPP_1	304	307	PF00568	0.745
DOC_USP7_MATH_1	230	234	PF00917	0.623
DOC_USP7_MATH_1	271	275	PF00917	0.624
DOC_WW_Pin1_4	124	129	PF00397	0.614
DOC_WW_Pin1_4	186	191	PF00397	0.353
DOC_WW_Pin1_4	23	28	PF00397	0.721
DOC_WW_Pin1_4	88	93	PF00397	0.516
LIG_14-3-3_CanoR_1	154	161	PF00244	0.625
LIG_Clathr_ClatBox_1	225	229	PF01394	0.509
LIG_FHA_1	166	172	PF00498	0.457
LIG_FHA_1	187	193	PF00498	0.362
LIG_FHA_1	251	257	PF00498	0.467
LIG_FHA_1	270	276	PF00498	0.661
LIG_FHA_1	79	85	PF00498	0.584
LIG_FHA_1	89	95	PF00498	0.453
LIG_FHA_2	176	182	PF00498	0.511
LIG_FHA_2	245	251	PF00498	0.554
LIG_LIR_Apic_2	136	141	PF02991	0.572
LIG_LIR_Gen_1	140	151	PF02991	0.462
LIG_LIR_Gen_1	191	200	PF02991	0.570
LIG_LIR_Gen_1	257	266	PF02991	0.467
LIG_LIR_LC3C_4	272	277	PF02991	0.618
LIG_LIR_Nem_3	140	146	PF02991	0.455
LIG_LIR_Nem_3	191	197	PF02991	0.558
LIG_LIR_Nem_3	199	203	PF02991	0.539
LIG_LYPXL_S_1	304	308	PF13949	0.684
LIG_LYPXL_yS_3	305	308	PF13949	0.658
LIG_PTB_Apo_2	174	181	PF02174	0.488
LIG_PTB_Phospho_1	174	180	PF10480	0.480
LIG_REV1ctd_RIR_1	135	140	PF16727	0.478
LIG_SH2_CRK	12	16	PF00017	0.648
LIG_SH2_CRK	39	43	PF00017	0.755
LIG_SH2_GRB2like	43	46	PF00017	0.813
LIG_SH2_NCK_1	143	147	PF00017	0.458
LIG_SH2_NCK_1	39	43	PF00017	0.755
LIG_SH2_SRC	143	146	PF00017	0.448
LIG_SH2_SRC	20	23	PF00017	0.800
LIG_SH2_SRC	39	42	PF00017	0.789
LIG_SH2_SRC	43	46	PF00017	0.648
LIG_SH2_STAP1	180	184	PF00017	0.517
LIG_SH2_STAT3	2	5	PF00017	0.647
LIG_SH2_STAT5	152	155	PF00017	0.428
LIG_SH3_3	204	210	PF00018	0.509
LIG_SH3_3	21	27	PF00018	0.657
LIG_SUMO_SIM_anti_2	204	210	PF11976	0.570
LIG_SUMO_SIM_anti_2	30	37	PF11976	0.777
LIG_SUMO_SIM_par_1	168	174	PF11976	0.436
LIG_SUMO_SIM_par_1	244	251	PF11976	0.623
LIG_UBA3_1	122	129	PF00899	0.595
MOD_CDK_SPK_2	124	129	PF00069	0.614
MOD_CK1_1	127	133	PF00069	0.521
MOD_CK1_1	199	205	PF00069	0.564
MOD_CK2_1	244	250	PF00069	0.664
MOD_Cter_Amidation	147	150	PF01082	0.486
MOD_GlcNHglycan	129	132	PF01048	0.301
MOD_GlcNHglycan	181	185	PF01048	0.528
MOD_GlcNHglycan	232	235	PF01048	0.395
MOD_GSK3_1	161	168	PF00069	0.529
MOD_GSK3_1	171	178	PF00069	0.431
MOD_GSK3_1	23	30	PF00069	0.695
MOD_GSK3_1	244	251	PF00069	0.632
MOD_GSK3_1	265	272	PF00069	0.635
MOD_GSK3_1	88	95	PF00069	0.503
MOD_N-GLC_1	159	164	PF02516	0.669
MOD_N-GLC_1	211	216	PF02516	0.309
MOD_N-GLC_1	79	84	PF02516	0.606
MOD_N-GLC_1	88	93	PF02516	0.255
MOD_NEK2_1	161	166	PF00069	0.529
MOD_NEK2_1	171	176	PF00069	0.419
MOD_NEK2_1	248	253	PF00069	0.604
MOD_NEK2_1	299	304	PF00069	0.767
MOD_PIKK_1	161	167	PF00454	0.527
MOD_PIKK_1	175	181	PF00454	0.497
MOD_PIKK_1	212	218	PF00454	0.532
MOD_PIKK_1	66	72	PF00454	0.801
MOD_PIKK_1	92	98	PF00454	0.509
MOD_PKA_2	153	159	PF00069	0.511
MOD_PKA_2	230	236	PF00069	0.595
MOD_Plk_1	212	218	PF00069	0.595
MOD_Plk_1	79	85	PF00069	0.522
MOD_Plk_1	92	98	PF00069	0.465
MOD_Plk_4	171	177	PF00069	0.527
MOD_Plk_4	271	277	PF00069	0.618
MOD_Plk_4	31	37	PF00069	0.768
MOD_Plk_4	80	86	PF00069	0.632
MOD_ProDKin_1	124	130	PF00069	0.614
MOD_ProDKin_1	186	192	PF00069	0.353
MOD_ProDKin_1	23	29	PF00069	0.724
MOD_ProDKin_1	88	94	PF00069	0.516
MOD_SUMO_for_1	210	213	PF00179	0.521
MOD_SUMO_rev_2	261	269	PF00179	0.533
TRG_ENDOCYTIC_2	143	146	PF00928	0.439
TRG_ENDOCYTIC_2	152	155	PF00928	0.431
TRG_ENDOCYTIC_2	194	197	PF00928	0.502
TRG_ENDOCYTIC_2	200	203	PF00928	0.496
TRG_ENDOCYTIC_2	305	308	PF00928	0.658
TRG_ER_diArg_1	149	151	PF00400	0.457
TRG_ER_diArg_1	71	74	PF00400	0.700

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I013	Leptomonas seymouri	76%	96%
A0A0S4JUP2	Bodo saltans	68%	77%
A0A1X0NMJ6	Trypanosomatidae	67%	98%
A0A3R7NIY6	Trypanosoma rangeli	65%	97%
A0A3S7WNY0	Leishmania donovani	99%	100%
A4H432	Leishmania braziliensis	94%	99%
A4HSB6	Leishmania infantum	99%	100%
C9ZYA9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	95%
E9AK98	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
V5BDL5	Trypanosoma cruzi	66%	99%