DNA repair protein RAD51 homolog

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA repair protein RAD51, putative

Species:

Leishmania major

UniProt:

O61127_LEIMA

TriTrypDb:

LmjF.28.0550 , LMJLV39_280011000 , LMJSD75_280011000

Length:

377

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000228	nuclear chromosome	6	2
GO:0000793	condensed chromosome	6	2
GO:0000794	condensed nuclear chromosome	7	2
GO:0005694	chromosome	5	2
GO:0043226	organelle	2	11
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	11
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	11
GO:0005634	nucleus	5	9
GO:0043227	membrane-bounded organelle	3	9
GO:0043231	intracellular membrane-bounded organelle	4	9

Expansion

Sequence features

O61127

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: O61127

PDB structure(s): 7qv8_A

Function

Biological processes
Term	Name	Level	Count
GO:0000730	DNA recombinase assembly	7	2
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006310	DNA recombination	5	11
GO:0006312	mitotic recombination	6	2
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0006996	organelle organization	4	2
GO:0007131	reciprocal meiotic recombination	3	2
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016043	cellular component organization	3	2
GO:0022402	cell cycle process	2	11
GO:0022414	reproductive process	1	2
GO:0022607	cellular component assembly	4	2
GO:0031297	replication fork processing	6	11
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0035825	homologous recombination	6	2
GO:0042148	strand invasion	5	2
GO:0043170	macromolecule metabolic process	3	11
GO:0043933	protein-containing complex organization	4	2
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0045005	DNA-templated DNA replication maintenance of fidelity	5	11
GO:0046483	heterocycle metabolic process	3	11
GO:0050896	response to stimulus	1	11
GO:0051276	chromosome organization	5	2
GO:0051716	cellular response to stimulus	2	11
GO:0065003	protein-containing complex assembly	5	2
GO:0065004	protein-DNA complex assembly	6	2
GO:0070192	chromosome organization involved in meiotic cell cycle	3	2
GO:0071704	organic substance metabolic process	2	11
GO:0071824	protein-DNA complex subunit organization	5	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:0090304	nucleic acid metabolic process	4	11
GO:0090735	DNA repair complex assembly	6	2
GO:0140527	reciprocal homologous recombination	7	2
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1902298	cell cycle DNA replication maintenance of fidelity	3	11
GO:1903046	meiotic cell cycle process	2	2
GO:1903047	mitotic cell cycle process	3	11
GO:1990426	mitotic recombination-dependent replication fork processing	5	11
GO:1990505	mitotic DNA replication maintenance of fidelity	4	11
GO:0000724	double-strand break repair via homologous recombination	7	9
GO:0000725	recombinational repair	6	9
GO:0006281	DNA repair	5	9
GO:0006302	double-strand break repair	6	9

Molecular functions
Term	Name	Level	Count
GO:0000150	DNA strand exchange activity	4	11
GO:0000166	nucleotide binding	3	11
GO:0003676	nucleic acid binding	3	11
GO:0003677	DNA binding	4	11
GO:0003690	double-stranded DNA binding	5	11
GO:0003697	single-stranded DNA binding	5	11
GO:0003824	catalytic activity	1	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0008094	ATP-dependent activity, acting on DNA	2	11
GO:0016462	pyrophosphatase activity	5	2
GO:0016787	hydrolase activity	2	2
GO:0016817	hydrolase activity, acting on acid anhydrides	3	2
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	2
GO:0016887	ATP hydrolysis activity	7	2
GO:0017076	purine nucleotide binding	4	11
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	2
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140097	catalytic activity, acting on DNA	3	11
GO:0140299	small molecule sensor activity	1	11
GO:0140612	DNA damage sensor activity	2	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:0140657	ATP-dependent activity	1	11
GO:0140664	ATP-dependent DNA damage sensor activity	3	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	10	12	PF00675	0.631
CLV_NRD_NRD_1	132	134	PF00675	0.211
CLV_NRD_NRD_1	340	342	PF00675	0.211
CLV_PCSK_KEX2_1	10	12	PF00082	0.602
CLV_PCSK_SKI1_1	252	256	PF00082	0.267
CLV_PCSK_SKI1_1	348	352	PF00082	0.235
CLV_PCSK_SKI1_1	95	99	PF00082	0.341
DEG_APCC_DBOX_1	251	259	PF00400	0.467
DEG_Nend_UBRbox_3	1	3	PF02207	0.588
DOC_MAPK_gen_1	133	139	PF00069	0.411
DOC_MAPK_gen_1	94	100	PF00069	0.332
DOC_USP7_MATH_1	16	20	PF00917	0.579
DOC_USP7_MATH_1	331	335	PF00917	0.417
DOC_USP7_MATH_1	54	58	PF00917	0.548
DOC_WW_Pin1_4	352	357	PF00397	0.414
LIG_14-3-3_CanoR_1	167	177	PF00244	0.411
LIG_14-3-3_CanoR_1	230	240	PF00244	0.411
LIG_14-3-3_CanoR_1	337	341	PF00244	0.411
LIG_APCC_ABBA_1	193	198	PF00400	0.411
LIG_APCC_ABBA_1	365	370	PF00400	0.411
LIG_Clathr_ClatBox_1	255	259	PF01394	0.467
LIG_EH1_1	58	66	PF00400	0.403
LIG_FHA_1	122	128	PF00498	0.411
LIG_FHA_1	154	160	PF00498	0.411
LIG_FHA_1	174	180	PF00498	0.411
LIG_FHA_1	301	307	PF00498	0.411
LIG_FHA_1	333	339	PF00498	0.411
LIG_IRF3_LxIS_1	179	186	PF10401	0.425
LIG_LIR_Gen_1	250	261	PF02991	0.411
LIG_LIR_Nem_3	160	166	PF02991	0.444
LIG_LIR_Nem_3	250	256	PF02991	0.446
LIG_LIR_Nem_3	362	368	PF02991	0.420
LIG_LIR_Nem_3	57	62	PF02991	0.531
LIG_LIR_Nem_3	80	86	PF02991	0.352
LIG_SH2_CRK	353	357	PF00017	0.411
LIG_SH2_SRC	368	371	PF00017	0.409
LIG_SH2_STAT5	196	199	PF00017	0.446
LIG_SH2_STAT5	84	87	PF00017	0.349
LIG_SUMO_SIM_anti_2	19	27	PF11976	0.690
LIG_SUMO_SIM_par_1	135	141	PF11976	0.411
LIG_SUMO_SIM_par_1	206	213	PF11976	0.425
LIG_SUMO_SIM_par_1	254	260	PF11976	0.411
LIG_TRAF2_1	18	21	PF00917	0.727
LIG_TRFH_1	215	219	PF08558	0.246
MOD_CK2_1	155	161	PF00069	0.289
MOD_Cter_Amidation	8	11	PF01082	0.590
MOD_GlcNHglycan	124	127	PF01048	0.284
MOD_GlcNHglycan	14	17	PF01048	0.618
MOD_GlcNHglycan	244	247	PF01048	0.276
MOD_GlcNHglycan	259	262	PF01048	0.266
MOD_GlcNHglycan	268	272	PF01048	0.246
MOD_GlcNHglycan	311	315	PF01048	0.246
MOD_GlcNHglycan	56	59	PF01048	0.554
MOD_GlcNHglycan	87	90	PF01048	0.401
MOD_GSK3_1	12	19	PF00069	0.616
MOD_GSK3_1	153	160	PF00069	0.278
MOD_GSK3_1	173	180	PF00069	0.246
MOD_GSK3_1	332	339	PF00069	0.258
MOD_N-GLC_1	54	59	PF02516	0.537
MOD_NEK2_1	121	126	PF00069	0.282
MOD_NEK2_1	138	143	PF00069	0.348
MOD_NEK2_1	173	178	PF00069	0.246
MOD_NEK2_1	242	247	PF00069	0.267
MOD_NEK2_1	285	290	PF00069	0.249
MOD_OFUCOSY	174	181	PF10250	0.246
MOD_PIKK_1	168	174	PF00454	0.246
MOD_PIKK_1	183	189	PF00454	0.265
MOD_PIKK_1	24	30	PF00454	0.684
MOD_PKA_2	12	18	PF00069	0.707
MOD_PKA_2	336	342	PF00069	0.246
MOD_Plk_2-3	155	161	PF00069	0.338
MOD_ProDKin_1	352	358	PF00069	0.249
MOD_SUMO_rev_2	104	108	PF00179	0.334
MOD_SUMO_rev_2	72	78	PF00179	0.313
TRG_DiLeu_BaEn_1	114	119	PF01217	0.332
TRG_DiLeu_BaEn_1	145	150	PF01217	0.246
TRG_ER_diArg_1	10	13	PF00400	0.713
TRG_NLS_MonoExtC_3	340	346	PF00514	0.246
TRG_Pf-PMV_PEXEL_1	76	80	PF00026	0.322

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P957	Leptomonas seymouri	53%	100%
A0A0N1IA48	Leptomonas seymouri	93%	100%
A0A0S4IRW2	Bodo saltans	28%	99%
A0A0S4J9C6	Bodo saltans	56%	100%
A0A0S4JEJ8	Bodo saltans	81%	100%
A0A1X0NYJ1	Trypanosomatidae	95%	100%
A0A1X0P517	Trypanosomatidae	55%	100%
A0A3Q8ID02	Leishmania donovani	99%	100%
A0A3Q8IGQ5	Leishmania donovani	51%	100%
A0A3R7M9F0	Trypanosoma rangeli	55%	100%
A0A422P1N0	Trypanosoma rangeli	88%	100%
A1KUQ0	Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 / DSM 15464 / FAM18)	27%	100%
A2SR54	Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)	38%	100%
A3CWU4	Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)	38%	100%
A3MXX9	Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)	44%	100%
A4FWV5	Methanococcus maripaludis (strain C5 / ATCC BAA-1333)	38%	100%
A4HG95	Leishmania braziliensis	95%	100%
A4HNF3	Leishmania braziliensis	49%	100%
A4I3C9	Leishmania infantum	99%	100%
A4IC25	Leishmania infantum	54%	100%
A4WN87	Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6)	45%	100%
A4YCN4	Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)	42%	100%
A5UMW0	Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)	41%	100%
A6VGG2	Methanococcus maripaludis (strain C7 / ATCC BAA-1331)	38%	100%
A8AB83	Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125)	42%	100%
A9AA90	Methanococcus maripaludis (strain C6 / ATCC BAA-1332)	38%	100%
A9AXA6	Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95)	26%	100%
B0R7Y4	Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)	38%	100%
B1YC14	Pyrobaculum neutrophilum (strain DSM 2338 / JCM 9278 / NBRC 100436 / V24Sta)	45%	100%
B2GB15	Limosilactobacillus fermentum (strain NBRC 3956 / LMG 18251)	26%	100%
B4RLX8	Neisseria gonorrhoeae (strain NCCP11945)	25%	100%
B8BM09	Oryza sativa subsp. indica	52%	100%
B8D610	Desulfurococcus amylolyticus (strain DSM 18924 / JCM 16383 / VKM B-2413 / 1221n)	41%	100%
C3MRI1	Sulfolobus islandicus (strain L.S.2.15 / Lassen #1)	41%	100%
C3MY77	Sulfolobus islandicus (strain M.14.25 / Kamchatka #1)	41%	100%
C3MZK6	Sulfolobus islandicus (strain M.16.27)	41%	100%
C3N7M8	Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1)	41%	100%
C3NFU5	Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2)	41%	100%
C4KIT6	Sulfolobus islandicus (strain M.16.4 / Kamchatka #3)	41%	100%
C9ZYH4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
E6MXY4	Neisseria meningitidis serogroup B / serotype 15 (strain H44/76)	27%	100%
E9AZL6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
E9B717	Leishmania mexicana (strain MHOM/GT/2001/U1103)	51%	100%
O15315	Homo sapiens	30%	98%
O27436	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	42%	100%
O29269	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	43%	100%
O35719	Mus musculus	31%	100%
O42634	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	47%	100%
O43502	Homo sapiens	28%	100%
O43542	Homo sapiens	28%	100%
O61128	Leishmania major	51%	100%
O73948	Methanococcus voltae	40%	100%
O74036	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	46%	100%
O77507	Oryctolagus cuniculus	69%	100%
O93748	Cenarchaeum symbiosum (strain A)	40%	95%
P0CW58	Methanococcus maripaludis	38%	100%
P0CW59	Methanococcus maripaludis (strain S2 / LL)	38%	100%
P0CW91	Aeropyrum pernix	43%	100%
P0CW92	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	42%	100%
P0DH59	Neisseria meningitidis serogroup B (strain MC58)	27%	100%
P21152	Neisseria gonorrhoeae	25%	100%
P25453	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	47%	100%
P25454	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	59%	94%
P33542	Aquifex pyrophilus	27%	100%
P36601	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	66%	100%
P37383	Gallus gallus	72%	100%
P37384	Lilium longiflorum	51%	100%
P50265	Candida albicans	48%	100%
P56987	Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491)	27%	100%
P70099	Cricetulus griseus	70%	100%
P94102	Arabidopsis thaliana	64%	100%
P96955	Neisseria polysaccharea	27%	100%
P97195	Neisseria lactamica	27%	100%
Q06609	Homo sapiens	70%	100%
Q08297	Mus musculus	70%	100%
Q08DH8	Bos taurus	28%	100%
Q0AHX4	Nitrosomonas eutropha (strain DSM 101675 / C91 / Nm57)	25%	100%
Q12UG7	Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)	41%	100%
Q14565	Homo sapiens	51%	100%
Q18EU1	Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)	36%	100%
Q27297	Drosophila melanogaster	63%	100%
Q2KJ94	Bos taurus	70%	100%
Q2NE95	Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)	41%	100%
Q39009	Arabidopsis thaliana	51%	100%
Q40134	Solanum lycopersicum	64%	100%
Q46A31	Methanosarcina barkeri (strain Fusaro / DSM 804)	41%	100%
Q48328	Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)	36%	100%
Q49593	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	42%	100%
Q4JAT5	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	40%	100%
Q54PJ7	Dictyostelium discoideum	29%	99%
Q55075	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	41%	100%
Q59560	Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)	25%	100%
Q59595	Neisseria cinerea	27%	100%
Q5F8M9	Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)	25%	100%
Q5YT00	Nocardia farcinica (strain IFM 10152)	25%	100%
Q61880	Mus musculus	51%	100%
Q67EU8	Zea mays	67%	100%
Q6L126	Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828)	40%	100%
Q74MX9	Nanoarchaeum equitans (strain Kin4-M)	44%	100%
Q7EAG4	Oryza sativa subsp. indica	52%	100%
Q7GBF7	Oryza sativa subsp. japonica	52%	100%
Q7GBF8	Oryza sativa subsp. japonica	52%	100%
Q8MKI8	Canis lupus familiaris	70%	100%
Q8PZN5	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	41%	100%
Q8R2J9	Cricetulus griseus	29%	100%
Q8TK71	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	42%	100%
Q8ZYR9	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	44%	100%
Q91917	Xenopus laevis	71%	100%
Q91918	Xenopus laevis	71%	100%
Q924H5	Mus musculus	27%	100%
Q96449	Glycine max	51%	100%
Q975Y1	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	40%	100%
Q97BJ9	Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)	42%	100%
Q99133	Ustilago maydis (strain 521 / FGSC 9021)	65%	100%
Q9HJ68	Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)	42%	100%
Q9HMM4	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	38%	100%
Q9SK02	Arabidopsis thaliana	32%	100%
Q9UUL2	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
Q9UWR5	Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)	45%	100%
Q9V233	Pyrococcus abyssi (strain GE5 / Orsay)	47%	100%
Q9XED7	Zea mays	67%	100%
Q9Y8J4	Desulfurococcus amylolyticus	41%	100%
V5B714	Trypanosoma cruzi	54%	100%
V5BKV4	Trypanosoma cruzi	85%	100%