DNA-(apurinic or apyrimidinic site) endonuclease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

apurinic/apyrimidinic endonuclease-redox protein

Species:

Leishmania major

UniProt:

O15922_LEIMA

TriTrypDb:

LmjF.16.0680 , LMJLV39_160012300 , LMJSD75_160012100

Length:

447

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

O15922

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: O15922

PDB structure(s): 2j63_A , 2j63_B

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006281	DNA repair	5	11
GO:0006284	base-excision repair	6	2
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0050896	response to stimulus	1	11
GO:0051716	cellular response to stimulus	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	8
GO:0003677	DNA binding	4	8
GO:0003824	catalytic activity	1	11
GO:0003906	DNA-(apurinic or apyrimidinic site) endonuclease activity	4	2
GO:0004518	nuclease activity	4	11
GO:0004519	endonuclease activity	5	11
GO:0004527	exonuclease activity	5	2
GO:0004529	DNA exonuclease activity	5	2
GO:0004536	DNA nuclease activity	4	2
GO:0005488	binding	1	11
GO:0008081	phosphoric diester hydrolase activity	5	2
GO:0008296	3'-5'-DNA exonuclease activity	7	2
GO:0008309	double-stranded DNA exodeoxyribonuclease activity	7	2
GO:0008311	double-stranded DNA 3'-5' DNA exonuclease activity	8	2
GO:0008408	3'-5' exonuclease activity	6	2
GO:0016787	hydrolase activity	2	11
GO:0016788	hydrolase activity, acting on ester bonds	3	11
GO:0016796	exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	2
GO:0016829	lyase activity	2	8
GO:0016895	DNA exonuclease activity, producing 5'-phosphomonoesters	6	2
GO:0042578	phosphoric ester hydrolase activity	4	2
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0097159	organic cyclic compound binding	2	8
GO:0140097	catalytic activity, acting on DNA	3	2
GO:0140640	catalytic activity, acting on a nucleic acid	2	2
GO:1901363	heterocyclic compound binding	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	173	177	PF00656	0.418
CLV_C14_Caspase3-7	82	86	PF00656	0.690
CLV_NRD_NRD_1	117	119	PF00675	0.483
CLV_NRD_NRD_1	73	75	PF00675	0.801
CLV_PCSK_KEX2_1	117	119	PF00082	0.490
CLV_PCSK_KEX2_1	197	199	PF00082	0.457
CLV_PCSK_KEX2_1	4	6	PF00082	0.788
CLV_PCSK_KEX2_1	73	75	PF00082	0.774
CLV_PCSK_PC1ET2_1	197	199	PF00082	0.457
CLV_PCSK_PC1ET2_1	4	6	PF00082	0.788
CLV_PCSK_SKI1_1	131	135	PF00082	0.357
CLV_PCSK_SKI1_1	141	145	PF00082	0.311
CLV_PCSK_SKI1_1	251	255	PF00082	0.312
CLV_PCSK_SKI1_1	269	273	PF00082	0.385
CLV_PCSK_SKI1_1	64	68	PF00082	0.690
DEG_APCC_DBOX_1	298	306	PF00400	0.312
DEG_COP1_1	310	318	PF00400	0.379
DEG_SPOP_SBC_1	14	18	PF00917	0.690
DEG_SPOP_SBC_1	264	268	PF00917	0.404
DOC_ANK_TNKS_1	238	245	PF00023	0.223
DOC_CYCLIN_yClb5_NLxxxL_5	402	410	PF00134	0.457
DOC_MAPK_JIP1_4	159	165	PF00069	0.414
DOC_PP1_RVXF_1	129	136	PF00149	0.344
DOC_PP1_RVXF_1	402	409	PF00149	0.332
DOC_PP4_FxxP_1	224	227	PF00568	0.424
DOC_PP4_FxxP_1	256	259	PF00568	0.411
DOC_PP4_FxxP_1	427	430	PF00568	0.312
DOC_USP7_MATH_1	101	105	PF00917	0.463
DOC_USP7_MATH_1	14	18	PF00917	0.690
DOC_USP7_MATH_1	225	229	PF00917	0.461
DOC_USP7_MATH_1	235	239	PF00917	0.741
DOC_USP7_MATH_1	264	268	PF00917	0.404
DOC_USP7_MATH_1	314	318	PF00917	0.800
DOC_USP7_MATH_1	320	324	PF00917	0.768
DOC_USP7_MATH_2	362	368	PF00917	0.312
DOC_USP7_UBL2_3	102	106	PF12436	0.534
DOC_WW_Pin1_4	19	24	PF00397	0.696
DOC_WW_Pin1_4	255	260	PF00397	0.420
DOC_WW_Pin1_4	323	328	PF00397	0.395
DOC_WW_Pin1_4	67	72	PF00397	0.778
LIG_14-3-3_CanoR_1	117	126	PF00244	0.461
LIG_14-3-3_CanoR_1	251	257	PF00244	0.332
LIG_14-3-3_CanoR_1	290	295	PF00244	0.361
LIG_14-3-3_CanoR_1	365	369	PF00244	0.312
LIG_14-3-3_CanoR_1	37	44	PF00244	0.690
LIG_14-3-3_CanoR_1	374	379	PF00244	0.312
LIG_14-3-3_CanoR_1	418	423	PF00244	0.379
LIG_14-3-3_CanoR_1	73	81	PF00244	0.736
LIG_BIR_II_1	1	5	PF00653	0.807
LIG_deltaCOP1_diTrp_1	107	115	PF00928	0.533
LIG_FHA_1	132	138	PF00498	0.323
LIG_FHA_1	16	22	PF00498	0.742
LIG_FHA_1	178	184	PF00498	0.404
LIG_FHA_1	252	258	PF00498	0.385
LIG_FHA_1	37	43	PF00498	0.693
LIG_FHA_1	373	379	PF00498	0.340
LIG_FHA_2	118	124	PF00498	0.504
LIG_FHA_2	80	86	PF00498	0.690
LIG_Integrin_isoDGR_2	400	402	PF01839	0.332
LIG_LIR_Apic_2	221	227	PF02991	0.457
LIG_LIR_Apic_2	254	259	PF02991	0.411
LIG_LIR_Apic_2	425	430	PF02991	0.312
LIG_LIR_Gen_1	107	116	PF02991	0.467
LIG_LIR_Gen_1	185	195	PF02991	0.424
LIG_LIR_Nem_3	107	112	PF02991	0.461
LIG_LIR_Nem_3	127	133	PF02991	0.192
LIG_LIR_Nem_3	185	190	PF02991	0.424
LIG_LIR_Nem_3	340	346	PF02991	0.292
LIG_LIR_Nem_3	379	384	PF02991	0.312
LIG_LIR_Nem_3	411	415	PF02991	0.312
LIG_MAD2	251	259	PF02301	0.411
LIG_NRBOX	139	145	PF00104	0.332
LIG_Pex14_1	408	412	PF04695	0.324
LIG_SH2_CRK	130	134	PF00017	0.454
LIG_SH2_STAP1	343	347	PF00017	0.312
LIG_SH2_STAT3	289	292	PF00017	0.385
LIG_SH2_STAT5	276	279	PF00017	0.312
LIG_SH2_STAT5	301	304	PF00017	0.385
LIG_SH2_STAT5	412	415	PF00017	0.385
LIG_SH2_STAT5	422	425	PF00017	0.312
LIG_SH3_3	187	193	PF00018	0.378
LIG_SH3_3	224	230	PF00018	0.457
LIG_SUMO_SIM_anti_2	265	275	PF11976	0.419
LIG_TRAF2_1	353	356	PF00917	0.345
LIG_TRAF2_1	361	364	PF00917	0.275
LIG_TYR_ITIM	410	415	PF00017	0.312
LIG_WRC_WIRS_1	253	258	PF05994	0.424
LIG_WRC_WIRS_1	291	296	PF05994	0.424
LIG_WRC_WIRS_1	391	396	PF05994	0.372
MOD_CDC14_SPxK_1	70	73	PF00782	0.768
MOD_CDK_SPxK_1	67	73	PF00069	0.768
MOD_CDK_SPxxK_3	67	74	PF00069	0.768
MOD_CK1_1	10	16	PF00069	0.823
MOD_CK1_1	17	23	PF00069	0.792
MOD_CK1_1	263	269	PF00069	0.433
MOD_CK1_1	323	329	PF00069	0.406
MOD_CK1_1	393	399	PF00069	0.424
MOD_CK2_1	101	107	PF00069	0.500
MOD_CK2_1	19	25	PF00069	0.768
MOD_CK2_1	294	300	PF00069	0.340
MOD_CK2_1	350	356	PF00069	0.345
MOD_CK2_1	364	370	PF00069	0.250
MOD_GlcNHglycan	148	151	PF01048	0.379
MOD_GlcNHglycan	200	203	PF01048	0.312
MOD_GlcNHglycan	262	265	PF01048	0.422
MOD_GlcNHglycan	279	282	PF01048	0.332
MOD_GlcNHglycan	316	319	PF01048	0.773
MOD_GlcNHglycan	322	325	PF01048	0.775
MOD_GlcNHglycan	356	360	PF01048	0.335
MOD_GlcNHglycan	52	55	PF01048	0.781
MOD_GlcNHglycan	76	79	PF01048	0.800
MOD_GlcNHglycan	9	12	PF01048	0.810
MOD_GSK3_1	10	17	PF00069	0.741
MOD_GSK3_1	198	205	PF00069	0.324
MOD_GSK3_1	251	258	PF00069	0.358
MOD_GSK3_1	260	267	PF00069	0.350
MOD_GSK3_1	290	297	PF00069	0.340
MOD_GSK3_1	364	371	PF00069	0.297
MOD_GSK3_1	414	421	PF00069	0.395
MOD_GSK3_1	428	435	PF00069	0.255
MOD_GSK3_1	45	52	PF00069	0.746
MOD_N-GLC_1	146	151	PF02516	0.457
MOD_N-GLC_1	177	182	PF02516	0.340
MOD_N-GLC_1	208	213	PF02516	0.443
MOD_NEK2_1	165	170	PF00069	0.322
MOD_NEK2_1	207	212	PF00069	0.383
MOD_NEK2_1	272	277	PF00069	0.321
MOD_NEK2_1	282	287	PF00069	0.296
MOD_NEK2_1	368	373	PF00069	0.297
MOD_NEK2_1	413	418	PF00069	0.300
MOD_NEK2_2	347	352	PF00069	0.424
MOD_PIKK_1	393	399	PF00454	0.457
MOD_PK_1	374	380	PF00069	0.457
MOD_PKA_1	117	123	PF00069	0.469
MOD_PKA_1	64	70	PF00069	0.792
MOD_PKA_1	73	79	PF00069	0.752
MOD_PKA_2	116	122	PF00069	0.448
MOD_PKA_2	218	224	PF00069	0.451
MOD_PKA_2	36	42	PF00069	0.718
MOD_PKA_2	364	370	PF00069	0.312
MOD_PKA_2	72	78	PF00069	0.776
MOD_PKB_1	5	13	PF00069	0.811
MOD_Plk_1	146	152	PF00069	0.457
MOD_Plk_1	177	183	PF00069	0.340
MOD_Plk_1	208	214	PF00069	0.443
MOD_Plk_2-3	185	191	PF00069	0.457
MOD_Plk_2-3	364	370	PF00069	0.385
MOD_Plk_4	185	191	PF00069	0.457
MOD_Plk_4	252	258	PF00069	0.296
MOD_Plk_4	272	278	PF00069	0.332
MOD_Plk_4	390	396	PF00069	0.332
MOD_Plk_4	418	424	PF00069	0.431
MOD_Plk_4	428	434	PF00069	0.414
MOD_ProDKin_1	19	25	PF00069	0.696
MOD_ProDKin_1	255	261	PF00069	0.420
MOD_ProDKin_1	323	329	PF00069	0.395
MOD_ProDKin_1	67	73	PF00069	0.778
MOD_SUMO_rev_2	161	170	PF00179	0.312
TRG_DiLeu_BaEn_4	248	254	PF01217	0.385
TRG_ENDOCYTIC_2	130	133	PF00928	0.456
TRG_ENDOCYTIC_2	187	190	PF00928	0.332
TRG_ENDOCYTIC_2	412	415	PF00928	0.312
TRG_ER_diArg_1	116	118	PF00400	0.500
TRG_Pf-PMV_PEXEL_1	239	243	PF00026	0.423
TRG_Pf-PMV_PEXEL_1	382	387	PF00026	0.442

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IIW7	Leptomonas seymouri	64%	100%
A0A0S4JDZ9	Bodo saltans	46%	100%
A0A3R7KY33	Trypanosoma rangeli	57%	99%
A0A3S7WTW9	Leishmania donovani	94%	100%
A0MTA1	Danio rerio	32%	100%
A1YES6	Gorilla gorilla gorilla	32%	100%
A1YFZ3	Pan paniscus	33%	100%
A2T6Y4	Pan troglodytes	33%	100%
A2T7I6	Pongo pygmaeus	33%	100%
A4H8J0	Leishmania braziliensis	83%	100%
C9ZW21	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	100%
E9AGN4	Leishmania infantum	95%	100%
E9AQM7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
P23196	Bos taurus	32%	100%
P27695	Homo sapiens	33%	100%
P28352	Mus musculus	32%	100%
P43138	Rattus norvegicus	32%	100%
P45951	Arabidopsis thaliana	32%	83%
P51173	Dictyostelium discoideum	34%	100%
P87175	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	85%
Q5XF07	Arabidopsis thaliana	23%	100%
Q68G58	Mus musculus	28%	87%
Q9UBZ4	Homo sapiens	27%	86%
V5AT33	Trypanosoma cruzi	57%	100%