Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

methyltransferase, putative

Species:

Leishmania braziliensis

UniProt:

MTR1_LEIBR

TriTrypDb:

LbrM.35.7020 , LBRM2903_350083300

Length:

398

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1

Expansion

Sequence features

A4HQI9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4HQI9

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006370	7-methylguanosine mRNA capping	8	12
GO:0006396	RNA processing	6	12
GO:0006397	mRNA processing	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009452	7-methylguanosine RNA capping	8	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0016556	mRNA modification	6	12
GO:0032259	methylation	2	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0036260	RNA capping	7	12
GO:0036451	cap mRNA methylation	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043414	macromolecule methylation	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0080009	mRNA methylation	5	12
GO:0090304	nucleic acid metabolic process	4	12
GO:0097309	cap1 mRNA methylation	7	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004483	mRNA (nucleoside-2'-O-)-methyltransferase activity	6	12
GO:0008168	methyltransferase activity	4	12
GO:0008171	O-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008174	mRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0003676	nucleic acid binding	3	9
GO:0005488	binding	1	9
GO:0097159	organic cyclic compound binding	2	9
GO:1901363	heterocyclic compound binding	2	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	394	398	PF00656	0.735
CLV_NRD_NRD_1	115	117	PF00675	0.303
CLV_NRD_NRD_1	14	16	PF00675	0.511
CLV_NRD_NRD_1	142	144	PF00675	0.379
CLV_NRD_NRD_1	243	245	PF00675	0.347
CLV_PCSK_KEX2_1	117	119	PF00082	0.350
CLV_PCSK_KEX2_1	14	16	PF00082	0.512
CLV_PCSK_PC1ET2_1	117	119	PF00082	0.350
CLV_PCSK_SKI1_1	38	42	PF00082	0.363
DEG_APCC_DBOX_1	48	56	PF00400	0.518
DEG_APCC_DBOX_1	70	78	PF00400	0.447
DEG_Nend_UBRbox_1	1	4	PF02207	0.583
DOC_MAPK_DCC_7	71	79	PF00069	0.451
DOC_MAPK_gen_1	71	79	PF00069	0.526
DOC_MAPK_MEF2A_6	201	209	PF00069	0.503
DOC_MAPK_MEF2A_6	290	297	PF00069	0.487
DOC_PP1_RVXF_1	5	12	PF00149	0.434
DOC_USP7_MATH_1	158	162	PF00917	0.584
DOC_USP7_MATH_1	23	27	PF00917	0.490
DOC_USP7_MATH_1	280	284	PF00917	0.476
DOC_USP7_MATH_1	388	392	PF00917	0.637
DOC_USP7_UBL2_3	140	144	PF12436	0.400
DOC_USP7_UBL2_3	241	245	PF12436	0.547
DOC_WW_Pin1_4	169	174	PF00397	0.562
DOC_WW_Pin1_4	255	260	PF00397	0.476
DOC_WW_Pin1_4	384	389	PF00397	0.735
LIG_14-3-3_CanoR_1	284	293	PF00244	0.476
LIG_BRCT_BRCA1_1	171	175	PF00533	0.518
LIG_BRCT_BRCA1_1	214	218	PF00533	0.400
LIG_BRCT_BRCA1_1	7	11	PF00533	0.533
LIG_Clathr_ClatBox_1	292	296	PF01394	0.562
LIG_deltaCOP1_diTrp_1	303	312	PF00928	0.452
LIG_deltaCOP1_diTrp_1	330	338	PF00928	0.508
LIG_FHA_1	176	182	PF00498	0.482
LIG_FHA_2	1	7	PF00498	0.550
LIG_FHA_2	378	384	PF00498	0.462
LIG_FHA_2	55	61	PF00498	0.476
LIG_LIR_Gen_1	101	111	PF02991	0.501
LIG_LIR_Gen_1	121	131	PF02991	0.345
LIG_LIR_Gen_1	164	173	PF02991	0.487
LIG_LIR_Nem_3	101	107	PF02991	0.501
LIG_LIR_Nem_3	121	127	PF02991	0.345
LIG_LIR_Nem_3	164	169	PF02991	0.479
LIG_LIR_Nem_3	255	260	PF02991	0.476
LIG_LIR_Nem_3	296	300	PF02991	0.565
LIG_LIR_Nem_3	303	308	PF02991	0.435
LIG_LIR_Nem_3	310	315	PF02991	0.385
LIG_MYND_1	324	328	PF01753	0.516
LIG_NRBOX	106	112	PF00104	0.581
LIG_Pex14_1	37	41	PF04695	0.392
LIG_Pex14_2	214	218	PF04695	0.487
LIG_Rb_pABgroove_1	248	256	PF01858	0.476
LIG_SH2_CRK	166	170	PF00017	0.430
LIG_SH2_CRK	272	276	PF00017	0.476
LIG_SH2_STAT3	31	34	PF00017	0.292
LIG_SH2_STAT5	266	269	PF00017	0.479
LIG_SH2_STAT5	291	294	PF00017	0.476
LIG_SH2_STAT5	308	311	PF00017	0.343
LIG_SH2_STAT5	31	34	PF00017	0.500
LIG_SH2_STAT5	67	70	PF00017	0.454
LIG_SH3_3	274	280	PF00018	0.460
LIG_SUMO_SIM_par_1	194	200	PF11976	0.522
LIG_SUMO_SIM_par_1	75	80	PF11976	0.466
LIG_TRAF2_1	366	369	PF00917	0.475
LIG_WRC_WIRS_1	254	259	PF05994	0.487
MOD_CDK_SPxxK_3	255	262	PF00069	0.476
MOD_CK1_1	258	264	PF00069	0.517
MOD_CK1_1	381	387	PF00069	0.737
MOD_CK1_1	391	397	PF00069	0.650
MOD_CK1_1	97	103	PF00069	0.562
MOD_CK2_1	362	368	PF00069	0.312
MOD_CK2_1	377	383	PF00069	0.456
MOD_CK2_1	387	393	PF00069	0.657
MOD_CK2_1	44	50	PF00069	0.370
MOD_Cter_Amidation	141	144	PF01082	0.381
MOD_GlcNHglycan	160	163	PF01048	0.340
MOD_GlcNHglycan	364	367	PF01048	0.355
MOD_GlcNHglycan	380	383	PF01048	0.465
MOD_GlcNHglycan	79	82	PF01048	0.509
MOD_GSK3_1	165	172	PF00069	0.483
MOD_GSK3_1	212	219	PF00069	0.503
MOD_GSK3_1	348	355	PF00069	0.427
MOD_GSK3_1	373	380	PF00069	0.550
MOD_GSK3_1	384	391	PF00069	0.508
MOD_GSK3_1	54	61	PF00069	0.497
MOD_GSK3_1	94	101	PF00069	0.533
MOD_N-GLC_1	345	350	PF02516	0.317
MOD_NEK2_1	105	110	PF00069	0.501
MOD_NEK2_1	175	180	PF00069	0.537
MOD_NEK2_1	188	193	PF00069	0.471
MOD_NEK2_1	253	258	PF00069	0.482
MOD_NEK2_1	270	275	PF00069	0.429
MOD_NEK2_1	345	350	PF00069	0.283
MOD_NEK2_1	98	103	PF00069	0.501
MOD_PIKK_1	132	138	PF00454	0.476
MOD_PIKK_1	54	60	PF00454	0.565
MOD_PKA_2	280	286	PF00069	0.487
MOD_PKA_2	378	384	PF00069	0.462
MOD_PKA_2	391	397	PF00069	0.515
MOD_Plk_1	216	222	PF00069	0.572
MOD_Plk_1	345	351	PF00069	0.344
MOD_Plk_1	5	11	PF00069	0.416
MOD_Plk_2-3	44	50	PF00069	0.373
MOD_Plk_2-3	5	11	PF00069	0.533
MOD_Plk_4	165	171	PF00069	0.481
MOD_ProDKin_1	169	175	PF00069	0.562
MOD_ProDKin_1	255	261	PF00069	0.476
MOD_ProDKin_1	384	390	PF00069	0.736
MOD_SUMO_rev_2	365	373	PF00179	0.565
TRG_DiLeu_BaEn_1	106	111	PF01217	0.581
TRG_ENDOCYTIC_2	166	169	PF00928	0.468
TRG_ENDOCYTIC_2	272	275	PF00928	0.466
TRG_ER_diArg_1	116	119	PF00400	0.400
TRG_ER_diArg_1	13	15	PF00400	0.498
TRG_NLS_MonoExtC_3	115	120	PF00514	0.511
TRG_NLS_MonoExtN_4	114	120	PF00514	0.551

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P800	Leptomonas seymouri	73%	99%
A0A0S4IIT9	Bodo saltans	45%	97%
A0A1X0NL91	Trypanosomatidae	56%	100%
A0A3S5IS12	Trypanosoma rangeli	54%	100%
A0A3S7XCC2	Leishmania donovani	85%	100%
A4HQI9	Leishmania braziliensis	100%	100%
D0A3N7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9AHZ8	Leishmania infantum	85%	100%
E9AUA2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%
Q38AH0	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	49%	100%
Q4E123	Trypanosoma cruzi (strain CL Brener)	55%	100%
Q4Q089	Leishmania major	83%	100%
V5BD10	Trypanosoma cruzi	54%	100%