Fumarate hydratase 2

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Fumarate hydratase class I, cytosolic

Species:

Leishmania major

UniProt:

FUM2_LEIMA

TriTrypDb:

LmjF.29.1960 , LMJLV39_290027300 , LMJSD75_290027500

Length:

568

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 12

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 10

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005777	peroxisome	6	2
GO:0005829	cytosol	2	2
GO:0020015	glycosome	7	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0042579	microbody	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0097014	ciliary plasm	5	2
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AE57

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AE57

PDB structure(s): 5l2r_A , 5l2r_B , 6msn_A , 6msn_B , 6unz_A , 6unz_B , 6unz_C , 6unz_D , 6unz_E , 6unz_F , 6unz_G , 6unz_H , 6uo0_A , 6uo0_B , 6uoi_A , 6uoi_B , 6uoj_A , 6uoj_B , 6up9_A , 6up9_B , 6upm_A , 6upm_B , 6upo_A , 6upo_B , 6uq8_A , 6uq8_B , 6uq9_A , 6uq9_B , 6uqb_A , 6uqb_B , 6uql_A , 6uql_B , 6uqm_A , 6uqm_B , 6uqn_A , 6uqn_B

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	2
GO:0006091	generation of precursor metabolites and energy	3	10
GO:0006106	fumarate metabolic process	7	2
GO:0006108	malate metabolic process	7	2
GO:0008152	metabolic process	1	10
GO:0009987	cellular process	1	10
GO:0019752	carboxylic acid metabolic process	5	2
GO:0043436	oxoacid metabolic process	4	2
GO:0043648	dicarboxylic acid metabolic process	6	2
GO:0044237	cellular metabolic process	2	10
GO:0044281	small molecule metabolic process	2	2
GO:0071704	organic substance metabolic process	2	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0004333	fumarate hydratase activity	5	10
GO:0005488	binding	1	10
GO:0005515	protein binding	2	2
GO:0016829	lyase activity	2	10
GO:0016835	carbon-oxygen lyase activity	3	10
GO:0016836	hydro-lyase activity	4	10
GO:0042802	identical protein binding	3	2
GO:0042803	protein homodimerization activity	4	2
GO:0043167	ion binding	2	10
GO:0043169	cation binding	3	10
GO:0046872	metal ion binding	4	10
GO:0046983	protein dimerization activity	3	2
GO:0051536	iron-sulfur cluster binding	3	10
GO:0051539	4 iron, 4 sulfur cluster binding	4	10
GO:0051540	metal cluster binding	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	106	110	PF00656	0.367
CLV_C14_Caspase3-7	430	434	PF00656	0.469
CLV_NRD_NRD_1	12	14	PF00675	0.760
CLV_NRD_NRD_1	90	92	PF00675	0.357
CLV_NRD_NRD_1	99	101	PF00675	0.333
CLV_PCSK_KEX2_1	12	14	PF00082	0.760
CLV_PCSK_KEX2_1	144	146	PF00082	0.347
CLV_PCSK_KEX2_1	392	394	PF00082	0.492
CLV_PCSK_KEX2_1	99	101	PF00082	0.354
CLV_PCSK_PC1ET2_1	144	146	PF00082	0.347
CLV_PCSK_PC1ET2_1	392	394	PF00082	0.492
CLV_PCSK_SKI1_1	228	232	PF00082	0.381
CLV_PCSK_SKI1_1	271	275	PF00082	0.347
CLV_PCSK_SKI1_1	350	354	PF00082	0.347
CLV_PCSK_SKI1_1	410	414	PF00082	0.397
CLV_PCSK_SKI1_1	496	500	PF00082	0.347
CLV_PCSK_SKI1_1	526	530	PF00082	0.492
CLV_PCSK_SKI1_1	65	69	PF00082	0.416
DEG_APCC_DBOX_1	349	357	PF00400	0.347
DOC_MAPK_RevD_3	378	393	PF00069	0.410
DOC_PP2B_LxvP_1	199	202	PF13499	0.424
DOC_PP4_FxxP_1	295	298	PF00568	0.410
DOC_USP7_MATH_1	105	109	PF00917	0.367
DOC_USP7_MATH_1	308	312	PF00917	0.367
DOC_USP7_MATH_1	460	464	PF00917	0.347
DOC_USP7_MATH_1	521	525	PF00917	0.492
DOC_USP7_UBL2_3	157	161	PF12436	0.392
DOC_USP7_UBL2_3	388	392	PF12436	0.286
DOC_USP7_UBL2_3	522	526	PF12436	0.469
DOC_WW_Pin1_4	442	447	PF00397	0.392
DOC_WW_Pin1_4	452	457	PF00397	0.298
LIG_14-3-3_CanoR_1	173	183	PF00244	0.347
LIG_14-3-3_CanoR_1	333	339	PF00244	0.347
LIG_14-3-3_CanoR_1	399	407	PF00244	0.369
LIG_14-3-3_CanoR_1	81	90	PF00244	0.418
LIG_APCC_ABBA_1	180	185	PF00400	0.347
LIG_APCC_ABBAyCdc20_2	220	226	PF00400	0.469
LIG_APCC_ABBAyCdc20_2	358	364	PF00400	0.424
LIG_APCC_Cbox_2	111	117	PF00515	0.347
LIG_BIR_II_1	1	5	PF00653	0.704
LIG_BRCT_BRCA1_1	31	35	PF00533	0.546
LIG_BRCT_BRCA1_1	460	464	PF00533	0.347
LIG_deltaCOP1_diTrp_1	539	549	PF00928	0.347
LIG_DLG_GKlike_1	471	478	PF00625	0.469
LIG_eIF4E_1	113	119	PF01652	0.469
LIG_FHA_1	114	120	PF00498	0.392
LIG_FHA_1	136	142	PF00498	0.349
LIG_FHA_1	175	181	PF00498	0.347
LIG_FHA_1	225	231	PF00498	0.347
LIG_FHA_1	270	276	PF00498	0.347
LIG_FHA_1	382	388	PF00498	0.363
LIG_FHA_1	414	420	PF00498	0.381
LIG_FHA_1	442	448	PF00498	0.492
LIG_FHA_2	149	155	PF00498	0.366
LIG_FHA_2	262	268	PF00498	0.347
LIG_FHA_2	284	290	PF00498	0.469
LIG_LIR_Gen_1	204	212	PF02991	0.353
LIG_LIR_Gen_1	28	37	PF02991	0.627
LIG_LIR_Gen_1	297	308	PF02991	0.347
LIG_LIR_Gen_1	45	54	PF02991	0.394
LIG_LIR_Gen_1	473	479	PF02991	0.403
LIG_LIR_Gen_1	485	490	PF02991	0.283
LIG_LIR_Nem_3	204	209	PF02991	0.353
LIG_LIR_Nem_3	28	34	PF02991	0.476
LIG_LIR_Nem_3	297	303	PF02991	0.347
LIG_LIR_Nem_3	45	50	PF02991	0.375
LIG_LIR_Nem_3	473	478	PF02991	0.403
LIG_LIR_Nem_3	485	489	PF02991	0.283
LIG_LIR_Nem_3	547	552	PF02991	0.425
LIG_PDZ_Class_1	563	568	PF00595	0.508
LIG_Pex14_2	31	35	PF04695	0.561
LIG_PTB_Apo_2	168	175	PF02174	0.469
LIG_PTB_Phospho_1	168	174	PF10480	0.469
LIG_REV1ctd_RIR_1	87	96	PF16727	0.367
LIG_RPA_C_Fungi	416	428	PF08784	0.367
LIG_SH2_CRK	169	173	PF00017	0.469
LIG_SH2_CRK	281	285	PF00017	0.347
LIG_SH2_CRK	449	453	PF00017	0.347
LIG_SH2_GRB2like	169	172	PF00017	0.397
LIG_SH2_PTP2	475	478	PF00017	0.367
LIG_SH2_PTP2	486	489	PF00017	0.367
LIG_SH2_STAP1	174	178	PF00017	0.353
LIG_SH2_STAP1	206	210	PF00017	0.469
LIG_SH2_STAP1	563	567	PF00017	0.450
LIG_SH2_STAT3	222	225	PF00017	0.469
LIG_SH2_STAT3	362	365	PF00017	0.492
LIG_SH2_STAT5	113	116	PF00017	0.469
LIG_SH2_STAT5	158	161	PF00017	0.359
LIG_SH2_STAT5	222	225	PF00017	0.469
LIG_SH2_STAT5	321	324	PF00017	0.347
LIG_SH2_STAT5	362	365	PF00017	0.498
LIG_SH2_STAT5	373	376	PF00017	0.212
LIG_SH2_STAT5	475	478	PF00017	0.347
LIG_SH2_STAT5	486	489	PF00017	0.347
LIG_SH2_STAT5	509	512	PF00017	0.347
LIG_SH2_STAT5	56	59	PF00017	0.433
LIG_SH3_3	34	40	PF00018	0.437
LIG_SH3_3	372	378	PF00018	0.384
LIG_SH3_5	202	206	PF00018	0.410
LIG_SUMO_SIM_anti_2	73	79	PF11976	0.430
LIG_UBA3_1	352	358	PF00899	0.433
MOD_CK1_1	283	289	PF00069	0.347
MOD_CK1_1	383	389	PF00069	0.458
MOD_CK1_1	485	491	PF00069	0.320
MOD_CK2_1	148	154	PF00069	0.347
MOD_CK2_1	201	207	PF00069	0.492
MOD_CK2_1	261	267	PF00069	0.347
MOD_Cter_Amidation	142	145	PF01082	0.347
MOD_GlcNHglycan	20	25	PF01048	0.587
MOD_GlcNHglycan	216	219	PF01048	0.331
MOD_GlcNHglycan	264	267	PF01048	0.361
MOD_GlcNHglycan	31	34	PF01048	0.542
MOD_GlcNHglycan	310	313	PF01048	0.367
MOD_GlcNHglycan	346	349	PF01048	0.347
MOD_GlcNHglycan	358	361	PF01048	0.473
MOD_GlcNHglycan	460	463	PF01048	0.347
MOD_GSK3_1	129	136	PF00069	0.424
MOD_GSK3_1	262	269	PF00069	0.366
MOD_GSK3_1	280	287	PF00069	0.347
MOD_GSK3_1	460	467	PF00069	0.347
MOD_GSK3_1	478	485	PF00069	0.347
MOD_GSK3_1	492	499	PF00069	0.256
MOD_N-GLC_1	356	361	PF02516	0.464
MOD_N-GLC_1	413	418	PF02516	0.469
MOD_N-GLC_1	492	497	PF02516	0.347
MOD_N-GLC_2	121	123	PF02516	0.347
MOD_NEK2_1	148	153	PF00069	0.367
MOD_NEK2_1	172	177	PF00069	0.361
MOD_NEK2_1	29	34	PF00069	0.631
MOD_NEK2_1	342	347	PF00069	0.347
MOD_NEK2_1	382	387	PF00069	0.494
MOD_NEK2_1	413	418	PF00069	0.354
MOD_NEK2_1	478	483	PF00069	0.492
MOD_NEK2_2	266	271	PF00069	0.347
MOD_NEK2_2	521	526	PF00069	0.492
MOD_PIKK_1	172	178	PF00454	0.347
MOD_PIKK_1	400	406	PF00454	0.367
MOD_PKA_2	172	178	PF00069	0.347
MOD_Plk_1	20	26	PF00069	0.628
MOD_Plk_1	266	272	PF00069	0.347
MOD_Plk_4	114	120	PF00069	0.347
MOD_Plk_4	413	419	PF00069	0.367
MOD_Plk_4	471	477	PF00069	0.347
MOD_Plk_4	482	488	PF00069	0.347
MOD_Plk_4	73	79	PF00069	0.402
MOD_ProDKin_1	442	448	PF00069	0.392
MOD_ProDKin_1	452	458	PF00069	0.298
MOD_SUMO_for_1	48	51	PF00179	0.523
MOD_SUMO_rev_2	154	159	PF00179	0.367
MOD_SUMO_rev_2	235	244	PF00179	0.347
MOD_SUMO_rev_2	386	394	PF00179	0.492
TRG_DiLeu_BaEn_1	226	231	PF01217	0.469
TRG_ENDOCYTIC_2	169	172	PF00928	0.469
TRG_ENDOCYTIC_2	206	209	PF00928	0.347
TRG_ENDOCYTIC_2	281	284	PF00928	0.347
TRG_ENDOCYTIC_2	475	478	PF00928	0.469
TRG_ENDOCYTIC_2	486	489	PF00928	0.469
TRG_ER_diArg_1	12	14	PF00400	0.636
TRG_ER_diArg_1	98	100	PF00400	0.354
TRG_NES_CRM1_1	190	205	PF08389	0.399
TRG_Pf-PMV_PEXEL_1	100	104	PF00026	0.350

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I631	Leptomonas seymouri	87%	100%
A0A0N1PCN7	Leptomonas seymouri	65%	100%
A0A1X0NIU7	Trypanosomatidae	63%	100%
A0A1X0NZZ8	Trypanosomatidae	76%	100%
A0A3R7N408	Trypanosoma rangeli	63%	100%
A0A3S5IRI9	Trypanosoma rangeli	74%	100%
A0A3S7WY69	Leishmania donovani	60%	100%
A2CIN0	Leishmania infantum	98%	100%
A2CIN8	Leishmania donovani	98%	100%
A4HD88	Leishmania braziliensis	63%	100%
A4HHJ6	Leishmania braziliensis	92%	100%
C9ZLE3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	72%	100%
D0A701	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	63%	100%
E9AE57	Leishmania major	100%	100%
E9AH43	Leishmania infantum	60%	100%
E9AWM1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	59%	100%
P0AC33	Escherichia coli (strain K12)	64%	100%
P0AC34	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	64%	100%
P14407	Escherichia coli (strain K12)	66%	100%
P40720	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	66%	98%
Q04718	Geobacillus stearothermophilus	27%	100%
Q4QAU9	Leishmania major	59%	100%
Q8X4P8	Escherichia coli O157:H7	64%	100%
V5BGH8	Trypanosoma cruzi	74%	100%
V5DSN1	Trypanosoma cruzi	66%	100%