Probable eukaryotic initiation factor 4A

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ATP-dependent RNA helicase, putative

Species:

Leishmania mexicana

UniProt:

E9B6I9_LEIMU

TriTrypDb:

LmxM.34.3100

Length:

926

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 6
Forrest at al. (procyclic)	no	yes: 6
Silverman et al.	no	yes: 2
Pissara et al.	yes	yes: 18

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 8

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 12

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005737	cytoplasm	2	1
GO:0010494	cytoplasmic stress granule	5	1
GO:0034399	nuclear periphery	2	1
GO:0035770	ribonucleoprotein granule	3	1
GO:0036464	cytoplasmic ribonucleoprotein granule	4	1
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0097165	nuclear stress granule	4	1
GO:0099080	supramolecular complex	2	1
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9B6I9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B6I9

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	13
GO:0003676	nucleic acid binding	3	13
GO:0003724	RNA helicase activity	3	12
GO:0003743	translation initiation factor activity	4	12
GO:0003824	catalytic activity	1	13
GO:0004386	helicase activity	2	13
GO:0005488	binding	1	13
GO:0005524	ATP binding	5	13
GO:0008135	translation factor activity, RNA binding	3	12
GO:0008186	ATP-dependent activity, acting on RNA	2	12
GO:0016787	hydrolase activity	2	13
GO:0017076	purine nucleotide binding	4	13
GO:0030554	adenyl nucleotide binding	5	13
GO:0032553	ribonucleotide binding	3	13
GO:0032555	purine ribonucleotide binding	4	13
GO:0032559	adenyl ribonucleotide binding	5	13
GO:0035639	purine ribonucleoside triphosphate binding	4	13
GO:0036094	small molecule binding	2	13
GO:0043167	ion binding	2	13
GO:0043168	anion binding	3	13
GO:0045182	translation regulator activity	1	12
GO:0090079	translation regulator activity, nucleic acid binding	2	12
GO:0097159	organic cyclic compound binding	2	13
GO:0097367	carbohydrate derivative binding	2	13
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	13
GO:0140657	ATP-dependent activity	1	13
GO:1901265	nucleoside phosphate binding	3	13
GO:1901363	heterocyclic compound binding	2	13
GO:0003723	RNA binding	4	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	902	906	PF00656	0.684
CLV_NRD_NRD_1	175	177	PF00675	0.715
CLV_NRD_NRD_1	219	221	PF00675	0.668
CLV_NRD_NRD_1	234	236	PF00675	0.629
CLV_NRD_NRD_1	261	263	PF00675	0.675
CLV_NRD_NRD_1	274	276	PF00675	0.624
CLV_NRD_NRD_1	313	315	PF00675	0.747
CLV_NRD_NRD_1	320	322	PF00675	0.655
CLV_NRD_NRD_1	342	344	PF00675	0.713
CLV_NRD_NRD_1	42	44	PF00675	0.741
CLV_NRD_NRD_1	422	424	PF00675	0.672
CLV_NRD_NRD_1	431	433	PF00675	0.512
CLV_NRD_NRD_1	546	548	PF00675	0.362
CLV_NRD_NRD_1	570	572	PF00675	0.358
CLV_NRD_NRD_1	679	681	PF00675	0.498
CLV_NRD_NRD_1	741	743	PF00675	0.373
CLV_NRD_NRD_1	757	759	PF00675	0.330
CLV_NRD_NRD_1	832	834	PF00675	0.516
CLV_NRD_NRD_1	876	878	PF00675	0.670
CLV_NRD_NRD_1	916	918	PF00675	0.695
CLV_PCSK_FUR_1	318	322	PF00082	0.696
CLV_PCSK_FUR_1	40	44	PF00082	0.751
CLV_PCSK_KEX2_1	175	177	PF00082	0.715
CLV_PCSK_KEX2_1	219	221	PF00082	0.668
CLV_PCSK_KEX2_1	233	235	PF00082	0.637
CLV_PCSK_KEX2_1	261	263	PF00082	0.675
CLV_PCSK_KEX2_1	274	276	PF00082	0.624
CLV_PCSK_KEX2_1	313	315	PF00082	0.747
CLV_PCSK_KEX2_1	320	322	PF00082	0.655
CLV_PCSK_KEX2_1	342	344	PF00082	0.713
CLV_PCSK_KEX2_1	42	44	PF00082	0.741
CLV_PCSK_KEX2_1	422	424	PF00082	0.734
CLV_PCSK_KEX2_1	431	433	PF00082	0.560
CLV_PCSK_KEX2_1	675	677	PF00082	0.436
CLV_PCSK_KEX2_1	679	681	PF00082	0.487
CLV_PCSK_KEX2_1	741	743	PF00082	0.369
CLV_PCSK_KEX2_1	757	759	PF00082	0.342
CLV_PCSK_KEX2_1	832	834	PF00082	0.539
CLV_PCSK_KEX2_1	875	877	PF00082	0.668
CLV_PCSK_KEX2_1	916	918	PF00082	0.695
CLV_PCSK_PC1ET2_1	675	677	PF00082	0.487
CLV_PCSK_PC7_1	171	177	PF00082	0.568
CLV_PCSK_SKI1_1	422	426	PF00082	0.605
CLV_PCSK_SKI1_1	468	472	PF00082	0.548
CLV_PCSK_SKI1_1	571	575	PF00082	0.399
CLV_PCSK_SKI1_1	607	611	PF00082	0.319
CLV_PCSK_SKI1_1	695	699	PF00082	0.490
CLV_PCSK_SKI1_1	833	837	PF00082	0.514
DEG_Nend_UBRbox_2	1	3	PF02207	0.715
DEG_SPOP_SBC_1	690	694	PF00917	0.567
DOC_ANK_TNKS_1	887	894	PF00023	0.641
DOC_CKS1_1	143	148	PF01111	0.563
DOC_CYCLIN_RxL_1	618	629	PF00134	0.519
DOC_CYCLIN_yCln2_LP_2	480	486	PF00134	0.497
DOC_MAPK_DCC_7	770	778	PF00069	0.562
DOC_MAPK_gen_1	447	455	PF00069	0.582
DOC_MAPK_gen_1	545	554	PF00069	0.562
DOC_MAPK_gen_1	679	687	PF00069	0.437
DOC_MAPK_gen_1	770	778	PF00069	0.557
DOC_MAPK_gen_1	829	838	PF00069	0.539
DOC_MAPK_MEF2A_6	502	509	PF00069	0.519
DOC_MAPK_MEF2A_6	547	556	PF00069	0.554
DOC_MAPK_MEF2A_6	829	838	PF00069	0.516
DOC_PP1_RVXF_1	569	576	PF00149	0.562
DOC_PP2B_LxvP_1	787	790	PF13499	0.556
DOC_PP4_FxxP_1	528	531	PF00568	0.519
DOC_PP4_FxxP_1	798	801	PF00568	0.562
DOC_USP7_MATH_1	111	115	PF00917	0.655
DOC_USP7_MATH_1	121	125	PF00917	0.638
DOC_USP7_MATH_1	149	153	PF00917	0.579
DOC_USP7_MATH_1	365	369	PF00917	0.746
DOC_USP7_MATH_1	379	383	PF00917	0.742
DOC_USP7_MATH_1	46	50	PF00917	0.741
DOC_USP7_MATH_1	899	903	PF00917	0.880
DOC_WW_Pin1_4	107	112	PF00397	0.661
DOC_WW_Pin1_4	126	131	PF00397	0.575
DOC_WW_Pin1_4	142	147	PF00397	0.631
DOC_WW_Pin1_4	366	371	PF00397	0.728
DOC_WW_Pin1_4	495	500	PF00397	0.501
DOC_WW_Pin1_4	539	544	PF00397	0.557
DOC_WW_Pin1_4	771	776	PF00397	0.519
LIG_14-3-3_CanoR_1	545	551	PF00244	0.517
LIG_14-3-3_CanoR_1	571	576	PF00244	0.563
LIG_14-3-3_CanoR_1	647	654	PF00244	0.559
LIG_14-3-3_CanoR_1	657	661	PF00244	0.568
LIG_14-3-3_CanoR_1	689	697	PF00244	0.571
LIG_APCC_ABBA_1	564	569	PF00400	0.558
LIG_APCC_ABBA_1	726	731	PF00400	0.558
LIG_BIR_III_4	115	119	PF00653	0.638
LIG_BRCT_BRCA1_1	662	666	PF00533	0.558
LIG_Clathr_ClatBox_1	727	731	PF01394	0.558
LIG_CtBP_PxDLS_1	462	466	PF00389	0.539
LIG_EH1_1	719	727	PF00400	0.562
LIG_FHA_1	557	563	PF00498	0.571
LIG_FHA_1	692	698	PF00498	0.547
LIG_FHA_1	772	778	PF00498	0.519
LIG_FHA_1	806	812	PF00498	0.538
LIG_FHA_2	148	154	PF00498	0.572
LIG_FHA_2	25	31	PF00498	0.730
LIG_FHA_2	295	301	PF00498	0.662
LIG_FHA_2	651	657	PF00498	0.578
LIG_FHA_2	97	103	PF00498	0.683
LIG_LIR_Apic_2	525	531	PF02991	0.579
LIG_LIR_Gen_1	456	466	PF02991	0.544
LIG_LIR_Gen_1	474	484	PF02991	0.565
LIG_LIR_Gen_1	578	585	PF02991	0.500
LIG_LIR_Gen_1	612	622	PF02991	0.572
LIG_LIR_Gen_1	646	654	PF02991	0.562
LIG_LIR_Nem_3	306	312	PF02991	0.632
LIG_LIR_Nem_3	344	348	PF02991	0.681
LIG_LIR_Nem_3	456	461	PF02991	0.580
LIG_LIR_Nem_3	474	479	PF02991	0.458
LIG_LIR_Nem_3	577	583	PF02991	0.511
LIG_LIR_Nem_3	612	617	PF02991	0.588
LIG_Pex14_2	662	666	PF04695	0.519
LIG_PTAP_UEV_1	907	912	PF05743	0.672
LIG_PTB_Apo_2	608	615	PF02174	0.522
LIG_PTB_Phospho_1	608	614	PF10480	0.562
LIG_SH2_CRK	458	462	PF00017	0.592
LIG_SH2_CRK	494	498	PF00017	0.536
LIG_SH2_CRK	503	507	PF00017	0.537
LIG_SH2_CRK	535	539	PF00017	0.565
LIG_SH2_CRK	581	585	PF00017	0.562
LIG_SH2_GRB2like	3	6	PF00017	0.633
LIG_SH2_NCK_1	458	462	PF00017	0.533
LIG_SH2_NCK_1	581	585	PF00017	0.562
LIG_SH2_SRC	304	307	PF00017	0.645
LIG_SH2_STAP1	581	585	PF00017	0.562
LIG_SH2_STAP1	807	811	PF00017	0.519
LIG_SH2_STAT5	3	6	PF00017	0.662
LIG_SH2_STAT5	614	617	PF00017	0.599
LIG_SH2_STAT5	648	651	PF00017	0.529
LIG_SH2_STAT5	677	680	PF00017	0.415
LIG_SH2_STAT5	683	686	PF00017	0.409
LIG_SH2_STAT5	807	810	PF00017	0.579
LIG_SH2_STAT5	841	844	PF00017	0.513
LIG_SH3_3	140	146	PF00018	0.663
LIG_SH3_3	148	154	PF00018	0.750
LIG_SH3_3	396	402	PF00018	0.868
LIG_SH3_3	552	558	PF00018	0.570
LIG_SH3_3	597	603	PF00018	0.579
LIG_SH3_3	905	911	PF00018	0.681
LIG_Sin3_3	477	484	PF02671	0.511
LIG_SUMO_SIM_par_1	505	510	PF11976	0.545
LIG_SUMO_SIM_par_1	774	781	PF11976	0.519
LIG_TRAF2_1	224	227	PF00917	0.656
LIG_TRAF2_1	354	357	PF00917	0.719
LIG_TYR_ITIM	533	538	PF00017	0.521
LIG_TYR_ITIM	579	584	PF00017	0.562
LIG_UBA3_1	670	675	PF00899	0.466
LIG_WRC_WIRS_1	25	30	PF05994	0.728
LIG_WRC_WIRS_1	345	350	PF05994	0.733
LIG_WRC_WIRS_1	614	619	PF05994	0.562
MOD_CDC14_SPxK_1	542	545	PF00782	0.569
MOD_CDK_SPxK_1	539	545	PF00069	0.569
MOD_CDK_SPxxK_3	495	502	PF00069	0.408
MOD_CK1_1	124	130	PF00069	0.650
MOD_CK1_1	451	457	PF00069	0.610
MOD_CK1_1	522	528	PF00069	0.568
MOD_CK1_1	54	60	PF00069	0.663
MOD_CK1_1	646	652	PF00069	0.519
MOD_CK1_1	749	755	PF00069	0.534
MOD_CK1_1	906	912	PF00069	0.761
MOD_CK2_1	149	155	PF00069	0.651
MOD_CK2_1	294	300	PF00069	0.658
MOD_CK2_1	650	656	PF00069	0.527
MOD_CK2_1	7	13	PF00069	0.684
MOD_CK2_1	96	102	PF00069	0.686
MOD_Cter_Amidation	217	220	PF01082	0.722
MOD_Cter_Amidation	272	275	PF01082	0.718
MOD_Cter_Amidation	340	343	PF01082	0.732
MOD_Cter_Amidation	40	43	PF01082	0.697
MOD_Cter_Amidation	873	876	PF01082	0.713
MOD_Cter_Amidation	914	917	PF01082	0.696
MOD_GlcNHglycan	133	136	PF01048	0.640
MOD_GlcNHglycan	147	150	PF01048	0.585
MOD_GlcNHglycan	200	203	PF01048	0.651
MOD_GlcNHglycan	229	232	PF01048	0.577
MOD_GlcNHglycan	252	255	PF01048	0.675
MOD_GlcNHglycan	285	288	PF01048	0.567
MOD_GlcNHglycan	356	360	PF01048	0.736
MOD_GlcNHglycan	396	399	PF01048	0.732
MOD_GlcNHglycan	410	413	PF01048	0.661
MOD_GlcNHglycan	450	453	PF01048	0.599
MOD_GlcNHglycan	509	512	PF01048	0.333
MOD_GlcNHglycan	521	524	PF01048	0.374
MOD_GlcNHglycan	55	59	PF01048	0.820
MOD_GlcNHglycan	617	620	PF01048	0.362
MOD_GlcNHglycan	662	665	PF01048	0.378
MOD_GlcNHglycan	888	891	PF01048	0.709
MOD_GlcNHglycan	908	911	PF01048	0.677
MOD_GSK3_1	107	114	PF00069	0.616
MOD_GSK3_1	120	127	PF00069	0.643
MOD_GSK3_1	145	152	PF00069	0.652
MOD_GSK3_1	366	373	PF00069	0.668
MOD_GSK3_1	646	653	PF00069	0.568
MOD_GSK3_1	656	663	PF00069	0.579
MOD_GSK3_1	691	698	PF00069	0.582
MOD_GSK3_1	777	784	PF00069	0.558
MOD_GSK3_1	899	906	PF00069	0.707
MOD_N-GLC_1	107	112	PF02516	0.648
MOD_N-GLC_1	250	255	PF02516	0.564
MOD_N-GLC_1	695	700	PF02516	0.543
MOD_NEK2_1	615	620	PF00069	0.545
MOD_NEK2_1	643	648	PF00069	0.511
MOD_NEK2_1	660	665	PF00069	0.629
MOD_NEK2_1	729	734	PF00069	0.530
MOD_PIKK_1	414	420	PF00454	0.643
MOD_PIKK_1	695	701	PF00454	0.559
MOD_PKA_1	422	428	PF00069	0.640
MOD_PKA_1	571	577	PF00069	0.599
MOD_PKA_1	7	13	PF00069	0.724
MOD_PKA_2	422	428	PF00069	0.661
MOD_PKA_2	448	454	PF00069	0.794
MOD_PKA_2	546	552	PF00069	0.519
MOD_PKA_2	646	652	PF00069	0.553
MOD_PKA_2	656	662	PF00069	0.565
MOD_PKA_2	906	912	PF00069	0.723
MOD_PKA_2	91	97	PF00069	0.741
MOD_Plk_2-3	24	30	PF00069	0.673
MOD_Plk_2-3	650	656	PF00069	0.562
MOD_Plk_4	656	662	PF00069	0.558
MOD_ProDKin_1	107	113	PF00069	0.662
MOD_ProDKin_1	126	132	PF00069	0.569
MOD_ProDKin_1	142	148	PF00069	0.631
MOD_ProDKin_1	366	372	PF00069	0.722
MOD_ProDKin_1	495	501	PF00069	0.408
MOD_ProDKin_1	539	545	PF00069	0.557
MOD_ProDKin_1	771	777	PF00069	0.519
MOD_SUMO_rev_2	2	10	PF00179	0.719
MOD_SUMO_rev_2	436	441	PF00179	0.594
MOD_SUMO_rev_2	464	473	PF00179	0.498
TRG_DiLeu_BaEn_1	595	600	PF01217	0.519
TRG_DiLeu_BaEn_1	861	866	PF01217	0.575
TRG_DiLeu_BaEn_2	655	661	PF01217	0.516
TRG_DiLeu_BaLyEn_6	709	714	PF01217	0.562
TRG_ENDOCYTIC_2	458	461	PF00928	0.590
TRG_ENDOCYTIC_2	535	538	PF00928	0.521
TRG_ENDOCYTIC_2	581	584	PF00928	0.562
TRG_ENDOCYTIC_2	614	617	PF00928	0.584
TRG_ENDOCYTIC_2	648	651	PF00928	0.562
TRG_ENDOCYTIC_2	682	685	PF00928	0.396
TRG_ER_diArg_1	219	221	PF00400	0.718
TRG_ER_diArg_1	232	235	PF00400	0.718
TRG_ER_diArg_1	260	262	PF00400	0.719
TRG_ER_diArg_1	274	276	PF00400	0.717
TRG_ER_diArg_1	312	314	PF00400	0.683
TRG_ER_diArg_1	319	321	PF00400	0.638
TRG_ER_diArg_1	40	43	PF00400	0.724
TRG_ER_diArg_1	421	423	PF00400	0.738
TRG_ER_diArg_1	430	432	PF00400	0.582
TRG_ER_diArg_1	678	680	PF00400	0.457
TRG_ER_diArg_1	741	743	PF00400	0.562
TRG_ER_diArg_1	832	834	PF00400	0.516
TRG_ER_diArg_1	875	877	PF00400	0.763
TRG_Pf-PMV_PEXEL_1	560	565	PF00026	0.359
TRG_Pf-PMV_PEXEL_1	741	746	PF00026	0.399
TRG_Pf-PMV_PEXEL_1	832	837	PF00026	0.521

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCW9	Leptomonas seymouri	66%	100%
A0A0S4JDN6	Bodo saltans	53%	100%
A0A3Q8IF94	Leishmania donovani	51%	100%
A0A3Q8IJ79	Leishmania donovani	92%	100%
A4HMX7	Leishmania braziliensis	79%	94%
A4I7K4	Leishmania infantum	51%	100%
A4IBK1	Leishmania infantum	92%	100%
E9AFD4	Leishmania major	94%	100%
E9AYQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9B2G1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	51%	100%
Q4Q5P5	Leishmania major	49%	100%