RNA polymerase-associated protein LEO1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA polymerase-associated protein LEO1, putative

Species:

Leishmania mexicana

UniProt:

E9B6G4_LEIMU

TriTrypDb:

LmxM.34.2850

Length:

555

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0008023	transcription elongation factor complex	3	12
GO:0016593	Cdc73/Paf1 complex	4	12
GO:0032991	protein-containing complex	1	12
GO:0140513	nuclear protein-containing complex	2	12
GO:0005634	nucleus	5	1
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B6G4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B6G4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006354	DNA-templated transcription elongation	6	12
GO:0006368	transcription elongation by RNA polymerase II	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009059	macromolecule biosynthetic process	4	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016570	histone modification	5	12
GO:0018130	heterocycle biosynthetic process	4	12
GO:0019438	aromatic compound biosynthetic process	4	12
GO:0019538	protein metabolic process	3	12
GO:0032774	RNA biosynthetic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034654	nucleobase-containing compound biosynthetic process	4	12
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044271	cellular nitrogen compound biosynthetic process	4	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901362	organic cyclic compound biosynthetic process	4	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901576	organic substance biosynthetic process	3	12
GO:0006355	regulation of DNA-templated transcription	6	1
GO:0006357	regulation of transcription by RNA polymerase II	7	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0009891	positive regulation of biosynthetic process	5	1
GO:0009893	positive regulation of metabolic process	4	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0010557	positive regulation of macromolecule biosynthetic process	6	1
GO:0010604	positive regulation of macromolecule metabolic process	5	1
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031325	positive regulation of cellular metabolic process	5	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0031328	positive regulation of cellular biosynthetic process	6	1
GO:0032784	regulation of DNA-templated transcription elongation	7	1
GO:0032786	positive regulation of DNA-templated transcription, elongation	8	1
GO:0032968	positive regulation of transcription elongation by RNA polymerase II	9	1
GO:0034243	regulation of transcription elongation by RNA polymerase II	8	1
GO:0045893	positive regulation of DNA-templated transcription	7	1
GO:0045935	positive regulation of nucleobase-containing compound metabolic process	6	1
GO:0045944	positive regulation of transcription by RNA polymerase II	8	1
GO:0048518	positive regulation of biological process	3	1
GO:0048522	positive regulation of cellular process	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051173	positive regulation of nitrogen compound metabolic process	5	1
GO:0051252	regulation of RNA metabolic process	5	1
GO:0051254	positive regulation of RNA metabolic process	6	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1902680	positive regulation of RNA biosynthetic process	7	1
GO:1903506	regulation of nucleic acid-templated transcription	7	1
GO:1903508	positive regulation of nucleic acid-templated transcription	8	1
GO:2001141	regulation of RNA biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0000993	RNA polymerase II complex binding	5	1
GO:0001098	basal transcription machinery binding	3	1
GO:0001099	basal RNA polymerase II transcription machinery binding	4	1
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0043175	RNA polymerase core enzyme binding	5	1
GO:0045309	protein phosphorylated amino acid binding	4	1
GO:0050815	phosphoserine residue binding	5	1
GO:0051219	phosphoprotein binding	3	1
GO:0070063	RNA polymerase binding	4	1
GO:0099122	RNA polymerase II C-terminal domain binding	6	1
GO:1990269	RNA polymerase II C-terminal domain phosphoserine binding	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	431	435	PF00656	0.730
CLV_C14_Caspase3-7	440	444	PF00656	0.673
CLV_NRD_NRD_1	291	293	PF00675	0.395
CLV_NRD_NRD_1	318	320	PF00675	0.419
CLV_NRD_NRD_1	342	344	PF00675	0.325
CLV_NRD_NRD_1	383	385	PF00675	0.570
CLV_PCSK_KEX2_1	150	152	PF00082	0.693
CLV_PCSK_KEX2_1	291	293	PF00082	0.370
CLV_PCSK_KEX2_1	341	343	PF00082	0.336
CLV_PCSK_KEX2_1	383	385	PF00082	0.572
CLV_PCSK_KEX2_1	399	401	PF00082	0.562
CLV_PCSK_PC1ET2_1	150	152	PF00082	0.693
CLV_PCSK_PC1ET2_1	341	343	PF00082	0.438
CLV_PCSK_PC1ET2_1	399	401	PF00082	0.697
CLV_PCSK_PC7_1	146	152	PF00082	0.578
CLV_PCSK_PC7_1	338	344	PF00082	0.365
CLV_PCSK_SKI1_1	102	106	PF00082	0.516
CLV_PCSK_SKI1_1	108	112	PF00082	0.510
CLV_PCSK_SKI1_1	153	157	PF00082	0.530
CLV_PCSK_SKI1_1	192	196	PF00082	0.436
CLV_PCSK_SKI1_1	271	275	PF00082	0.365
CLV_PCSK_SKI1_1	338	342	PF00082	0.369
CLV_PCSK_SKI1_1	348	352	PF00082	0.287
CLV_PCSK_SKI1_1	384	388	PF00082	0.516
CLV_PCSK_SKI1_1	487	491	PF00082	0.491
CLV_PCSK_SKI1_1	71	75	PF00082	0.515
DEG_APCC_DBOX_1	486	494	PF00400	0.465
DEG_APCC_DBOX_1	70	78	PF00400	0.507
DOC_CKS1_1	195	200	PF01111	0.421
DOC_CYCLIN_RxL_1	484	491	PF00134	0.426
DOC_CYCLIN_yCln2_LP_2	161	167	PF00134	0.542
DOC_MAPK_gen_1	102	112	PF00069	0.572
DOC_MAPK_gen_1	341	352	PF00069	0.302
DOC_MAPK_gen_1	399	409	PF00069	0.594
DOC_PP1_RVXF_1	214	221	PF00149	0.280
DOC_PP2B_LxvP_1	161	164	PF13499	0.518
DOC_USP7_MATH_1	155	159	PF00917	0.547
DOC_USP7_MATH_1	309	313	PF00917	0.233
DOC_USP7_MATH_1	485	489	PF00917	0.465
DOC_USP7_MATH_1	499	503	PF00917	0.436
DOC_USP7_UBL2_3	341	345	PF12436	0.365
DOC_WW_Pin1_4	153	158	PF00397	0.516
DOC_WW_Pin1_4	194	199	PF00397	0.491
DOC_WW_Pin1_4	512	517	PF00397	0.556
DOC_WW_Pin1_4	9	14	PF00397	0.689
LIG_14-3-3_CanoR_1	170	174	PF00244	0.389
LIG_14-3-3_CanoR_1	203	211	PF00244	0.360
LIG_14-3-3_CanoR_1	219	227	PF00244	0.286
LIG_14-3-3_CanoR_1	348	353	PF00244	0.464
LIG_14-3-3_CanoR_1	455	465	PF00244	0.671
LIG_14-3-3_CanoR_1	487	495	PF00244	0.491
LIG_14-3-3_CanoR_1	59	67	PF00244	0.498
LIG_AP2alpha_2	422	424	PF02296	0.725
LIG_APCC_ABBA_1	110	115	PF00400	0.564
LIG_APCC_ABBA_1	87	92	PF00400	0.626
LIG_APCC_ABBAyCdc20_2	170	176	PF00400	0.386
LIG_BIR_II_1	1	5	PF00653	0.574
LIG_BIR_III_3	1	5	PF00653	0.501
LIG_BRCT_BRCA1_1	189	193	PF00533	0.600
LIG_FHA_1	197	203	PF00498	0.276
LIG_FHA_1	224	230	PF00498	0.291
LIG_FHA_1	241	247	PF00498	0.405
LIG_FHA_1	248	254	PF00498	0.374
LIG_FHA_1	272	278	PF00498	0.421
LIG_FHA_1	530	536	PF00498	0.446
LIG_FHA_1	538	544	PF00498	0.489
LIG_FHA_1	68	74	PF00498	0.576
LIG_FHA_1	9	15	PF00498	0.716
LIG_FHA_2	135	141	PF00498	0.679
LIG_FHA_2	18	24	PF00498	0.650
LIG_FHA_2	349	355	PF00498	0.454
LIG_FHA_2	513	519	PF00498	0.565
LIG_FHA_2	59	65	PF00498	0.479
LIG_GBD_Chelix_1	482	490	PF00786	0.524
LIG_LIR_Apic_2	312	318	PF02991	0.388
LIG_LIR_Gen_1	498	507	PF02991	0.548
LIG_LIR_Gen_1	508	516	PF02991	0.595
LIG_LIR_Nem_3	190	196	PF02991	0.551
LIG_LIR_Nem_3	477	482	PF02991	0.528
LIG_LIR_Nem_3	498	503	PF02991	0.548
LIG_NRBOX	485	491	PF00104	0.475
LIG_Pex14_2	48	52	PF04695	0.459
LIG_SH2_CRK	479	483	PF00017	0.418
LIG_SH2_SRC	113	116	PF00017	0.555
LIG_SH2_SRC	187	190	PF00017	0.503
LIG_SH2_SRC	305	308	PF00017	0.249
LIG_SH2_STAP1	305	309	PF00017	0.171
LIG_SH2_STAT5	119	122	PF00017	0.543
LIG_SH2_STAT5	187	190	PF00017	0.508
LIG_SH2_STAT5	196	199	PF00017	0.352
LIG_SH3_3	162	168	PF00018	0.598
LIG_SH3_3	176	182	PF00018	0.330
LIG_SH3_3	2	8	PF00018	0.637
LIG_SUMO_SIM_anti_2	232	239	PF11976	0.473
LIG_TRAF2_1	238	241	PF00917	0.405
LIG_WRC_WIRS_1	442	447	PF05994	0.547
MOD_CK1_1	212	218	PF00069	0.401
MOD_CK1_1	221	227	PF00069	0.241
MOD_CK1_1	239	245	PF00069	0.298
MOD_CK1_1	472	478	PF00069	0.665
MOD_CK1_1	488	494	PF00069	0.460
MOD_CK2_1	134	140	PF00069	0.663
MOD_CK2_1	320	326	PF00069	0.340
MOD_CK2_1	348	354	PF00069	0.444
MOD_GlcNHglycan	121	124	PF01048	0.640
MOD_GlcNHglycan	307	310	PF01048	0.229
MOD_GlcNHglycan	369	372	PF01048	0.526
MOD_GlcNHglycan	414	417	PF01048	0.714
MOD_GSK3_1	134	141	PF00069	0.569
MOD_GSK3_1	151	158	PF00069	0.397
MOD_GSK3_1	232	239	PF00069	0.316
MOD_GSK3_1	305	312	PF00069	0.260
MOD_GSK3_1	437	444	PF00069	0.714
MOD_GSK3_1	456	463	PF00069	0.768
MOD_GSK3_1	499	506	PF00069	0.453
MOD_GSK3_1	508	515	PF00069	0.456
MOD_N-GLC_1	204	209	PF02516	0.329
MOD_N-GLC_1	367	372	PF02516	0.357
MOD_N-GLC_1	394	399	PF02516	0.591
MOD_N-GLC_1	456	461	PF02516	0.534
MOD_N-GLC_1	512	517	PF02516	0.624
MOD_NEK2_1	138	143	PF00069	0.547
MOD_NEK2_1	40	45	PF00069	0.644
MOD_NEK2_1	537	542	PF00069	0.409
MOD_NEK2_1	67	72	PF00069	0.488
MOD_NEK2_2	223	228	PF00069	0.280
MOD_OFUCOSY	180	185	PF10250	0.578
MOD_PIKK_1	236	242	PF00454	0.376
MOD_PIKK_1	26	32	PF00454	0.731
MOD_PIKK_1	394	400	PF00454	0.482
MOD_PIKK_1	488	494	PF00454	0.445
MOD_PIKK_1	537	543	PF00454	0.508
MOD_PK_1	320	326	PF00069	0.313
MOD_PKA_2	169	175	PF00069	0.402
MOD_PKA_2	218	224	PF00069	0.316
MOD_PKA_2	432	438	PF00069	0.751
MOD_PKA_2	58	64	PF00069	0.506
MOD_Plk_1	139	145	PF00069	0.611
MOD_Plk_1	240	246	PF00069	0.410
MOD_Plk_2-3	279	285	PF00069	0.365
MOD_Plk_2-3	432	438	PF00069	0.640
MOD_Plk_2-3	441	447	PF00069	0.508
MOD_Plk_4	155	161	PF00069	0.527
MOD_Plk_4	232	238	PF00069	0.410
MOD_Plk_4	505	511	PF00069	0.510
MOD_ProDKin_1	153	159	PF00069	0.503
MOD_ProDKin_1	194	200	PF00069	0.403
MOD_ProDKin_1	512	518	PF00069	0.558
MOD_ProDKin_1	9	15	PF00069	0.688
MOD_SUMO_for_1	470	473	PF00179	0.675
MOD_SUMO_for_1	543	546	PF00179	0.537
MOD_SUMO_rev_2	464	470	PF00179	0.623
MOD_SUMO_rev_2	98	106	PF00179	0.583
TRG_DiLeu_BaEn_2	188	194	PF01217	0.582
TRG_ENDOCYTIC_2	479	482	PF00928	0.425
TRG_ER_diArg_1	291	294	PF00400	0.395
TRG_ER_diArg_1	342	344	PF00400	0.436
TRG_ER_diArg_1	382	384	PF00400	0.566
TRG_ER_diArg_1	400	403	PF00400	0.508
TRG_NLS_Bipartite_1	383	403	PF00514	0.626
TRG_NLS_MonoExtC_3	340	345	PF00514	0.437
TRG_NLS_MonoExtC_3	398	404	PF00514	0.695
TRG_NLS_MonoExtN_4	317	323	PF00514	0.421
TRG_NLS_MonoExtN_4	338	345	PF00514	0.365
TRG_NLS_MonoExtN_4	396	403	PF00514	0.690
TRG_Pf-PMV_PEXEL_1	322	326	PF00026	0.421
TRG_Pf-PMV_PEXEL_1	342	346	PF00026	0.421
TRG_Pf-PMV_PEXEL_1	487	492	PF00026	0.555
TRG_Pf-PMV_PEXEL_1	526	530	PF00026	0.562

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMF7	Leptomonas seymouri	65%	97%
A0A0S4JFB0	Bodo saltans	27%	85%
A0A1X0P5N0	Trypanosomatidae	39%	92%
A0A3Q8IMF9	Leishmania donovani	93%	100%
A0A3R7P349	Trypanosoma rangeli	38%	97%
A4HMV2	Leishmania braziliensis	83%	100%
A4IBH3	Leishmania infantum	93%	100%
C9ZZ69	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	96%
E9AFA9	Leishmania major	92%	100%
V5BD17	Trypanosoma cruzi	37%	92%