Putative phosphoinositide-binding protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Autophagy-related protein 24

Species:

Leishmania mexicana

UniProt:

E9B6C2_LEIMU

TriTrypDb:

LmxM.34.2420

Length:

418

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 4
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005768	endosome	7	1
GO:0012506	vesicle membrane	4	1
GO:0016020	membrane	2	1
GO:0030659	cytoplasmic vesicle membrane	5	1
GO:0030666	endocytic vesicle membrane	5	1
GO:0031090	organelle membrane	3	1
GO:0031410	cytoplasmic vesicle	6	1
GO:0031982	vesicle	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0097708	intracellular vesicle	5	1
GO:0098588	bounding membrane of organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B6C2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B6C2

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006897	endocytosis	5	1
GO:0006898	receptor-mediated endocytosis	6	1
GO:0006914	autophagy	3	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009987	cellular process	1	1
GO:0016192	vesicle-mediated transport	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0061919	process utilizing autophagic mechanism	2	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	12
GO:0005543	phospholipid binding	3	12
GO:0008289	lipid binding	2	12
GO:0035091	phosphatidylinositol binding	4	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	105	107	PF00675	0.338
CLV_NRD_NRD_1	110	112	PF00675	0.307
CLV_NRD_NRD_1	237	239	PF00675	0.279
CLV_NRD_NRD_1	273	275	PF00675	0.415
CLV_NRD_NRD_1	47	49	PF00675	0.312
CLV_PCSK_FUR_1	235	239	PF00082	0.410
CLV_PCSK_KEX2_1	105	107	PF00082	0.418
CLV_PCSK_KEX2_1	110	112	PF00082	0.417
CLV_PCSK_KEX2_1	237	239	PF00082	0.283
CLV_PCSK_KEX2_1	47	49	PF00082	0.280
CLV_PCSK_PC7_1	106	112	PF00082	0.410
CLV_PCSK_SKI1_1	110	114	PF00082	0.285
CLV_PCSK_SKI1_1	226	230	PF00082	0.392
CLV_PCSK_SKI1_1	385	389	PF00082	0.274
CLV_PCSK_SKI1_1	410	414	PF00082	0.532
DEG_APCC_DBOX_1	109	117	PF00400	0.291
DEG_APCC_DBOX_1	225	233	PF00400	0.310
DEG_APCC_DBOX_1	305	313	PF00400	0.291
DEG_APCC_DBOX_1	315	323	PF00400	0.291
DEG_APCC_DBOX_1	409	417	PF00400	0.532
DEG_SPOP_SBC_1	164	168	PF00917	0.694
DOC_CYCLIN_yClb1_LxF_4	345	351	PF00134	0.154
DOC_CYCLIN_yClb5_NLxxxL_5	226	232	PF00134	0.392
DOC_MAPK_gen_1	389	396	PF00069	0.389
DOC_MAPK_MEF2A_6	389	398	PF00069	0.384
DOC_SPAK_OSR1_1	149	153	PF12202	0.480
DOC_USP7_MATH_1	165	169	PF00917	0.697
DOC_USP7_MATH_1	174	178	PF00917	0.709
DOC_USP7_MATH_1	3	7	PF00917	0.510
DOC_USP7_UBL2_3	385	389	PF12436	0.274
DOC_WW_Pin1_4	151	156	PF00397	0.346
LIG_14-3-3_CanoR_1	105	110	PF00244	0.410
LIG_14-3-3_CanoR_1	149	155	PF00244	0.576
LIG_14-3-3_CanoR_1	237	246	PF00244	0.343
LIG_14-3-3_CanoR_1	290	296	PF00244	0.400
LIG_14-3-3_CanoR_1	47	55	PF00244	0.401
LIG_APCC_ABBA_1	373	378	PF00400	0.386
LIG_BIR_II_1	1	5	PF00653	0.547
LIG_BRCT_BRCA1_1	369	373	PF00533	0.392
LIG_FHA_1	287	293	PF00498	0.329
LIG_FHA_1	31	37	PF00498	0.405
LIG_FHA_2	366	372	PF00498	0.372
LIG_FHA_2	48	54	PF00498	0.392
LIG_FHA_2	93	99	PF00498	0.392
LIG_LIR_Apic_2	26	31	PF02991	0.462
LIG_LIR_Gen_1	370	378	PF02991	0.412
LIG_LIR_Gen_1	405	413	PF02991	0.423
LIG_LIR_Gen_1	50	59	PF02991	0.275
LIG_LIR_Gen_1	62	72	PF02991	0.273
LIG_LIR_Nem_3	103	107	PF02991	0.410
LIG_LIR_Nem_3	24	30	PF02991	0.450
LIG_LIR_Nem_3	249	253	PF02991	0.366
LIG_LIR_Nem_3	370	376	PF02991	0.413
LIG_LIR_Nem_3	405	409	PF02991	0.292
LIG_LIR_Nem_3	50	55	PF02991	0.275
LIG_LIR_Nem_3	62	68	PF02991	0.273
LIG_PDZ_Class_2	413	418	PF00595	0.365
LIG_Pex14_2	246	250	PF04695	0.274
LIG_Pex14_2	369	373	PF04695	0.405
LIG_SH2_CRK	104	108	PF00017	0.410
LIG_SH2_CRK	118	122	PF00017	0.410
LIG_SH2_CRK	65	69	PF00017	0.335
LIG_SH2_PTP2	28	31	PF00017	0.440
LIG_SH2_SRC	28	31	PF00017	0.457
LIG_SH2_STAP1	280	284	PF00017	0.366
LIG_SH2_STAT5	214	217	PF00017	0.274
LIG_SH2_STAT5	28	31	PF00017	0.457
LIG_SH2_STAT5	298	301	PF00017	0.331
LIG_SH2_STAT5	318	321	PF00017	0.122
LIG_SH2_STAT5	49	52	PF00017	0.432
LIG_SH2_STAT5	65	68	PF00017	0.205
LIG_SH3_2	144	149	PF14604	0.537
LIG_SH3_2	69	74	PF14604	0.274
LIG_SH3_3	141	147	PF00018	0.566
LIG_SH3_3	149	155	PF00018	0.574
LIG_SH3_3	66	72	PF00018	0.287
LIG_SH3_3	8	14	PF00018	0.373
LIG_SUMO_SIM_par_1	392	397	PF11976	0.410
LIG_TRAF2_1	155	158	PF00917	0.633
LIG_TYR_ITIM	102	107	PF00017	0.410
LIG_TYR_ITIM	116	121	PF00017	0.410
LIG_WW_3	146	150	PF00397	0.526
MOD_CK1_1	167	173	PF00069	0.717
MOD_CK1_1	185	191	PF00069	0.519
MOD_CK1_1	241	247	PF00069	0.392
MOD_CK1_1	293	299	PF00069	0.411
MOD_CK2_1	151	157	PF00069	0.544
MOD_CK2_1	365	371	PF00069	0.403
MOD_CK2_1	394	400	PF00069	0.390
MOD_CK2_1	47	53	PF00069	0.291
MOD_CK2_1	87	93	PF00069	0.406
MOD_Cter_Amidation	272	275	PF01082	0.410
MOD_GlcNHglycan	167	170	PF01048	0.677
MOD_GlcNHglycan	176	179	PF01048	0.602
MOD_GlcNHglycan	184	187	PF01048	0.558
MOD_GlcNHglycan	240	243	PF01048	0.419
MOD_GlcNHglycan	313	316	PF01048	0.324
MOD_GlcNHglycan	331	334	PF01048	0.321
MOD_GlcNHglycan	89	92	PF01048	0.296
MOD_GSK3_1	159	166	PF00069	0.607
MOD_GSK3_1	19	26	PF00069	0.485
MOD_GSK3_1	204	211	PF00069	0.518
MOD_GSK3_1	217	224	PF00069	0.300
MOD_GSK3_1	286	293	PF00069	0.403
MOD_GSK3_1	394	401	PF00069	0.358
MOD_GSK3_1	47	54	PF00069	0.403
MOD_GSK3_1	87	94	PF00069	0.305
MOD_LATS_1	236	242	PF00433	0.392
MOD_N-GLC_1	285	290	PF02516	0.385
MOD_NEK2_1	150	155	PF00069	0.504
MOD_NEK2_1	228	233	PF00069	0.440
MOD_NEK2_1	23	28	PF00069	0.483
MOD_NEK2_1	246	251	PF00069	0.361
MOD_NEK2_1	291	296	PF00069	0.395
MOD_NEK2_1	329	334	PF00069	0.461
MOD_NEK2_1	394	399	PF00069	0.370
MOD_NEK2_1	412	417	PF00069	0.485
MOD_NEK2_1	59	64	PF00069	0.392
MOD_NEK2_1	87	92	PF00069	0.348
MOD_PIKK_1	12	18	PF00454	0.533
MOD_PIKK_1	185	191	PF00454	0.593
MOD_PIKK_1	196	202	PF00454	0.436
MOD_PKA_1	105	111	PF00069	0.410
MOD_PKA_1	47	53	PF00069	0.291
MOD_PKA_2	105	111	PF00069	0.410
MOD_PKA_2	305	311	PF00069	0.274
MOD_PKA_2	47	53	PF00069	0.366
MOD_Plk_1	204	210	PF00069	0.517
MOD_Plk_1	23	29	PF00069	0.475
MOD_Plk_1	286	292	PF00069	0.410
MOD_Plk_1	365	371	PF00069	0.410
MOD_Plk_1	394	400	PF00069	0.410
MOD_Plk_2-3	73	79	PF00069	0.410
MOD_Plk_4	228	234	PF00069	0.176
MOD_Plk_4	23	29	PF00069	0.520
MOD_Plk_4	293	299	PF00069	0.396
MOD_Plk_4	59	65	PF00069	0.396
MOD_ProDKin_1	151	157	PF00069	0.348
MOD_SUMO_for_1	402	405	PF00179	0.410
MOD_SUMO_rev_2	378	387	PF00179	0.278
TRG_DiLeu_BaEn_1	287	292	PF01217	0.410
TRG_DiLeu_BaEn_4	257	263	PF01217	0.410
TRG_ENDOCYTIC_2	104	107	PF00928	0.410
TRG_ENDOCYTIC_2	118	121	PF00928	0.410
TRG_ENDOCYTIC_2	298	301	PF00928	0.274
TRG_ENDOCYTIC_2	49	52	PF00928	0.274
TRG_ENDOCYTIC_2	65	68	PF00928	0.274
TRG_ER_diArg_1	104	106	PF00400	0.274
TRG_ER_diArg_1	109	111	PF00400	0.274
TRG_ER_diArg_1	235	238	PF00400	0.291
TRG_ER_diArg_1	47	49	PF00400	0.405
TRG_NES_CRM1_1	386	400	PF08389	0.410
TRG_Pf-PMV_PEXEL_1	226	230	PF00026	0.348

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IBS5	Leptomonas seymouri	77%	98%
A0A0S4KLA7	Bodo saltans	34%	100%
A0A1X0P5K9	Trypanosomatidae	34%	100%
A0A3Q8IV97	Leishmania donovani	97%	100%
A0A3R7L2W7	Trypanosoma rangeli	34%	99%
A4HMR6	Leishmania braziliensis	92%	100%
A4IBF2	Leishmania infantum	97%	100%
C9ZZB2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
E9AF66	Leishmania major	97%	100%
O60749	Homo sapiens	24%	81%
P0C220	Macaca fascicularis	23%	80%
Q05B62	Bos taurus	23%	80%
Q13596	Homo sapiens	24%	80%
Q2TBW7	Bos taurus	23%	81%
Q4R503	Macaca fascicularis	24%	80%
Q5R9A9	Pongo abelii	23%	80%
Q5RFP8	Pongo abelii	24%	80%
Q99N27	Rattus norvegicus	24%	80%
Q9CWK8	Mus musculus	24%	81%
Q9WV80	Mus musculus	24%	80%
V5B3T2	Trypanosoma cruzi	34%	99%