rRNA adenine N(6)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

rRNA dimethyltransferase, putative

Species:

Leishmania mexicana

UniProt:

E9B5V7_LEIMU

TriTrypDb:

LmxM.34.0770

Length:

464

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B5V7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B5V7

Function

Biological processes
Term	Name	Level	Count
GO:0000154	rRNA modification	6	1
GO:0001510	RNA methylation	4	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006364	rRNA processing	8	1
GO:0006396	RNA processing	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0016072	rRNA metabolic process	7	1
GO:0031167	rRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000179	rRNA (adenine-N6,N6-)-dimethyltransferase activity	6	12
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008170	N-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008649	rRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016433	rRNA (adenine) methyltransferase activity	6	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140102	catalytic activity, acting on a rRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	155	157	PF00675	0.452
CLV_NRD_NRD_1	213	215	PF00675	0.478
CLV_PCSK_FUR_1	211	215	PF00082	0.608
CLV_PCSK_KEX2_1	155	157	PF00082	0.452
CLV_PCSK_KEX2_1	213	215	PF00082	0.476
CLV_PCSK_SKI1_1	181	185	PF00082	0.473
CLV_PCSK_SKI1_1	286	290	PF00082	0.345
CLV_PCSK_SKI1_1	383	387	PF00082	0.238
CLV_PCSK_SKI1_1	53	57	PF00082	0.211
CLV_PCSK_SKI1_1	92	96	PF00082	0.219
DEG_ODPH_VHL_1	309	322	PF01847	0.436
DEG_SPOP_SBC_1	4	8	PF00917	0.604
DOC_ANK_TNKS_1	28	35	PF00023	0.384
DOC_ANK_TNKS_1	343	350	PF00023	0.514
DOC_CKS1_1	73	78	PF01111	0.478
DOC_CYCLIN_RxL_1	89	96	PF00134	0.411
DOC_MAPK_MEF2A_6	53	60	PF00069	0.411
DOC_MAPK_NFAT4_5	53	61	PF00069	0.411
DOC_USP7_MATH_1	166	170	PF00917	0.663
DOC_USP7_MATH_1	173	177	PF00917	0.633
DOC_USP7_MATH_1	200	204	PF00917	0.592
DOC_USP7_MATH_1	215	219	PF00917	0.605
DOC_USP7_MATH_1	280	284	PF00917	0.405
DOC_USP7_MATH_1	311	315	PF00917	0.429
DOC_USP7_MATH_1	4	8	PF00917	0.698
DOC_USP7_UBL2_3	36	40	PF12436	0.368
DOC_WW_Pin1_4	156	161	PF00397	0.645
DOC_WW_Pin1_4	238	243	PF00397	0.685
DOC_WW_Pin1_4	456	461	PF00397	0.680
DOC_WW_Pin1_4	7	12	PF00397	0.531
DOC_WW_Pin1_4	72	77	PF00397	0.495
LIG_14-3-3_CanoR_1	214	220	PF00244	0.685
LIG_14-3-3_CanoR_1	319	323	PF00244	0.418
LIG_14-3-3_CanoR_1	331	337	PF00244	0.411
LIG_14-3-3_CanoR_1	383	388	PF00244	0.423
LIG_14-3-3_CanoR_1	70	76	PF00244	0.422
LIG_Actin_WH2_2	369	385	PF00022	0.463
LIG_APCC_ABBA_1	111	116	PF00400	0.514
LIG_BIR_III_4	192	196	PF00653	0.586
LIG_BRCT_BRCA1_1	313	317	PF00533	0.422
LIG_FHA_1	128	134	PF00498	0.533
LIG_FHA_1	270	276	PF00498	0.419
LIG_FHA_1	372	378	PF00498	0.440
LIG_FHA_1	59	65	PF00498	0.441
LIG_FHA_1	89	95	PF00498	0.415
LIG_FHA_2	298	304	PF00498	0.405
LIG_FHA_2	333	339	PF00498	0.411
LIG_FHA_2	343	349	PF00498	0.411
LIG_FHA_2	412	418	PF00498	0.422
LIG_FHA_2	72	78	PF00498	0.480
LIG_LIR_Gen_1	314	323	PF02991	0.411
LIG_LIR_Nem_3	144	149	PF02991	0.346
LIG_LIR_Nem_3	314	320	PF02991	0.411
LIG_PTB_Apo_2	120	127	PF02174	0.523
LIG_PTB_Apo_2	140	147	PF02174	0.222
LIG_PTB_Apo_2	240	247	PF02174	0.538
LIG_PTB_Apo_2	262	269	PF02174	0.411
LIG_REV1ctd_RIR_1	325	335	PF16727	0.514
LIG_SH2_CRK	39	43	PF00017	0.496
LIG_SH2_GRB2like	121	124	PF00017	0.496
LIG_SH2_SRC	219	222	PF00017	0.687
LIG_SH2_STAP1	50	54	PF00017	0.422
LIG_SH2_STAT3	402	405	PF00017	0.411
LIG_SH2_STAT5	121	124	PF00017	0.427
LIG_SH2_STAT5	67	70	PF00017	0.514
LIG_SH3_3	14	20	PF00018	0.600
LIG_SH3_3	151	157	PF00018	0.508
LIG_SH3_3	22	28	PF00018	0.432
LIG_SH3_3	230	236	PF00018	0.490
LIG_SUMO_SIM_anti_2	137	144	PF11976	0.490
LIG_SUMO_SIM_anti_2	318	324	PF11976	0.411
LIG_SUMO_SIM_anti_2	417	422	PF11976	0.463
LIG_SUMO_SIM_par_1	350	355	PF11976	0.478
LIG_SUMO_SIM_par_1	414	419	PF11976	0.436
LIG_TRAF2_1	160	163	PF00917	0.667
LIG_TRAF2_1	441	444	PF00917	0.493
LIG_TRAF2_2	441	446	PF00917	0.548
LIG_UBA3_1	55	59	PF00899	0.411
MOD_CDK_SPxK_1	72	78	PF00069	0.478
MOD_CK1_1	222	228	PF00069	0.772
MOD_CK1_1	318	324	PF00069	0.411
MOD_CK1_1	5	11	PF00069	0.634
MOD_CK1_1	66	72	PF00069	0.441
MOD_CK1_1	93	99	PF00069	0.411
MOD_CK2_1	297	303	PF00069	0.451
MOD_CK2_1	342	348	PF00069	0.444
MOD_CK2_1	416	422	PF00069	0.429
MOD_DYRK1A_RPxSP_1	156	160	PF00069	0.553
MOD_GlcNHglycan	143	146	PF01048	0.545
MOD_GlcNHglycan	168	171	PF01048	0.657
MOD_GlcNHglycan	176	179	PF01048	0.655
MOD_GlcNHglycan	202	205	PF01048	0.573
MOD_GlcNHglycan	227	230	PF01048	0.651
MOD_GSK3_1	2	9	PF00069	0.592
MOD_GSK3_1	215	222	PF00069	0.725
MOD_GSK3_1	311	318	PF00069	0.411
MOD_GSK3_1	383	390	PF00069	0.505
MOD_GSK3_1	68	75	PF00069	0.422
MOD_GSK3_1	86	93	PF00069	0.439
MOD_N-GLC_1	119	124	PF02516	0.296
MOD_NEK2_1	141	146	PF00069	0.463
MOD_NEK2_1	246	251	PF00069	0.552
MOD_NEK2_1	332	337	PF00069	0.459
MOD_NEK2_1	365	370	PF00069	0.539
MOD_NEK2_1	416	421	PF00069	0.482
MOD_NEK2_1	58	63	PF00069	0.441
MOD_NEK2_1	68	73	PF00069	0.321
MOD_NEK2_1	90	95	PF00069	0.411
MOD_PIKK_1	280	286	PF00454	0.514
MOD_PIKK_1	297	303	PF00454	0.338
MOD_PIKK_1	434	440	PF00454	0.420
MOD_PKA_2	215	221	PF00069	0.621
MOD_PKA_2	318	324	PF00069	0.418
MOD_PKA_2	69	75	PF00069	0.422
MOD_Plk_1	337	343	PF00069	0.411
MOD_Plk_1	365	371	PF00069	0.496
MOD_Plk_1	416	422	PF00069	0.486
MOD_Plk_4	318	324	PF00069	0.417
MOD_Plk_4	332	338	PF00069	0.483
MOD_Plk_4	366	372	PF00069	0.440
MOD_Plk_4	411	417	PF00069	0.424
MOD_Plk_4	63	69	PF00069	0.514
MOD_ProDKin_1	156	162	PF00069	0.652
MOD_ProDKin_1	238	244	PF00069	0.684
MOD_ProDKin_1	456	462	PF00069	0.680
MOD_ProDKin_1	7	13	PF00069	0.529
MOD_ProDKin_1	72	78	PF00069	0.495
MOD_SUMO_for_1	258	261	PF00179	0.411
MOD_SUMO_for_1	30	33	PF00179	0.361
TRG_DiLeu_BaLyEn_6	205	210	PF01217	0.602
TRG_ER_diArg_1	154	156	PF00400	0.443
TRG_ER_diArg_1	210	213	PF00400	0.442
TRG_NES_CRM1_1	398	410	PF08389	0.514
TRG_Pf-PMV_PEXEL_1	208	212	PF00026	0.524
TRG_Pf-PMV_PEXEL_1	78	82	PF00026	0.228

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HXZ3	Leptomonas seymouri	79%	86%
A0A0S4IZP9	Bodo saltans	46%	87%
A0A1X0NJH8	Trypanosomatidae	56%	87%
A0A3R7NNC2	Trypanosoma rangeli	58%	97%
A0A3S7X8W9	Leishmania donovani	93%	100%
A4HMA9	Leishmania braziliensis	88%	100%
A4IAX5	Leishmania infantum	92%	100%
D0A280	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	99%
E9AEQ2	Leishmania major	94%	100%
Q121Q5	Polaromonas sp. (strain JS666 / ATCC BAA-500)	24%	100%
V5BN79	Trypanosoma cruzi	61%	97%