Defective in cullin neddylation protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Cullin binding, putative

Species:

Leishmania mexicana

UniProt:

E9B5M2_LEIMU

TriTrypDb:

LmxM.33.4270

Length:

236

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0000151	ubiquitin ligase complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990234	transferase complex	3	1
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B5M2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B5M2

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009893	positive regulation of metabolic process	4	1
GO:0010604	positive regulation of macromolecule metabolic process	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0019538	protein metabolic process	3	1
GO:0031396	regulation of protein ubiquitination	8	1
GO:0031398	positive regulation of protein ubiquitination	9	1
GO:0031399	regulation of protein modification process	6	1
GO:0031401	positive regulation of protein modification process	7	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043085	positive regulation of catalytic activity	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044093	positive regulation of molecular function	3	1
GO:0044238	primary metabolic process	2	1
GO:0045116	protein neddylation	7	1
GO:0048518	positive regulation of biological process	3	1
GO:0050789	regulation of biological process	2	1
GO:0050790	regulation of catalytic activity	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051173	positive regulation of nitrogen compound metabolic process	5	1
GO:0051246	regulation of protein metabolic process	5	1
GO:0051247	positive regulation of protein metabolic process	6	1
GO:0051338	regulation of transferase activity	4	1
GO:0051347	positive regulation of transferase activity	5	1
GO:0051438	regulation of ubiquitin-protein transferase activity	5	1
GO:0051443	positive regulation of ubiquitin-protein transferase activity	6	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0065009	regulation of molecular function	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1903320	regulation of protein modification by small protein conjugation or removal	7	1
GO:1903322	positive regulation of protein modification by small protein conjugation or removal	8	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0031624	ubiquitin conjugating enzyme binding	5	1
GO:0032182	ubiquitin-like protein binding	3	1
GO:0044390	ubiquitin-like protein conjugating enzyme binding	4	1
GO:0097602	cullin family protein binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	153	155	PF00675	0.451
CLV_PCSK_KEX2_1	153	155	PF00082	0.447
CLV_PCSK_SKI1_1	127	131	PF00082	0.190
CLV_PCSK_SKI1_1	153	157	PF00082	0.386
DEG_Nend_UBRbox_1	1	4	PF02207	0.728
DOC_MAPK_gen_1	176	185	PF00069	0.159
DOC_MAPK_gen_1	37	47	PF00069	0.529
DOC_MAPK_gen_1	6	13	PF00069	0.694
DOC_MAPK_MEF2A_6	40	47	PF00069	0.490
DOC_MAPK_NFAT4_5	40	48	PF00069	0.495
DOC_USP7_MATH_1	219	223	PF00917	0.351
DOC_USP7_MATH_1	22	26	PF00917	0.501
DOC_USP7_MATH_1	27	31	PF00917	0.490
DOC_USP7_MATH_1	77	81	PF00917	0.313
DOC_USP7_MATH_1	9	13	PF00917	0.683
LIG_14-3-3_CanoR_1	119	129	PF00244	0.373
LIG_14-3-3_CanoR_1	153	158	PF00244	0.429
LIG_14-3-3_CanoR_1	210	218	PF00244	0.316
LIG_14-3-3_CanoR_1	6	12	PF00244	0.640
LIG_BRCT_BRCA1_1	9	13	PF00533	0.637
LIG_deltaCOP1_diTrp_1	192	201	PF00928	0.302
LIG_EH1_1	109	117	PF00400	0.485
LIG_FHA_1	156	162	PF00498	0.451
LIG_FHA_1	203	209	PF00498	0.243
LIG_FHA_2	97	103	PF00498	0.396
LIG_LIR_Gen_1	135	144	PF02991	0.301
LIG_LIR_Gen_1	162	168	PF02991	0.344
LIG_LIR_Gen_1	44	53	PF02991	0.401
LIG_LIR_Nem_3	162	167	PF02991	0.302
LIG_LIR_Nem_3	192	197	PF02991	0.313
LIG_LIR_Nem_3	44	50	PF02991	0.360
LIG_PCNA_yPIPBox_3	78	89	PF02747	0.198
LIG_Pex14_1	164	168	PF04695	0.315
LIG_PTB_Apo_2	138	145	PF02174	0.327
LIG_PTB_Phospho_1	138	144	PF10480	0.351
LIG_REV1ctd_RIR_1	165	174	PF16727	0.373
LIG_SH2_CRK	140	144	PF00017	0.310
LIG_SH2_SRC	108	111	PF00017	0.533
LIG_SH2_STAP1	108	112	PF00017	0.269
LIG_SH2_STAT5	142	145	PF00017	0.359
LIG_SH2_STAT5	146	149	PF00017	0.318
LIG_TYR_ITIM	138	143	PF00017	0.419
LIG_UBA3_1	86	95	PF00899	0.416
MOD_CK1_1	111	117	PF00069	0.222
MOD_CK1_1	56	62	PF00069	0.525
MOD_CK2_1	118	124	PF00069	0.269
MOD_CK2_1	38	44	PF00069	0.444
MOD_CK2_1	76	82	PF00069	0.512
MOD_CK2_1	96	102	PF00069	0.391
MOD_CMANNOS	177	180	PF00535	0.315
MOD_GlcNHglycan	161	164	PF01048	0.393
MOD_GlcNHglycan	215	218	PF01048	0.342
MOD_GlcNHglycan	221	224	PF01048	0.342
MOD_GlcNHglycan	24	27	PF01048	0.693
MOD_GlcNHglycan	54	58	PF01048	0.573
MOD_GlcNHglycan	62	65	PF01048	0.525
MOD_GSK3_1	155	162	PF00069	0.276
MOD_GSK3_1	18	25	PF00069	0.582
MOD_GSK3_1	199	206	PF00069	0.382
MOD_GSK3_1	209	216	PF00069	0.398
MOD_GSK3_1	229	236	PF00069	0.353
MOD_GSK3_1	56	63	PF00069	0.504
MOD_NEK2_1	118	123	PF00069	0.435
MOD_NEK2_1	155	160	PF00069	0.448
MOD_NEK2_1	203	208	PF00069	0.406
MOD_NEK2_1	53	58	PF00069	0.560
MOD_NEK2_1	90	95	PF00069	0.377
MOD_NEK2_2	103	108	PF00069	0.395
MOD_PIKK_1	90	96	PF00454	0.455
MOD_PKA_1	153	159	PF00069	0.451
MOD_PKA_2	118	124	PF00069	0.434
MOD_PKA_2	153	159	PF00069	0.451
MOD_PKA_2	209	215	PF00069	0.316
MOD_PKB_1	15	23	PF00069	0.663
MOD_Plk_1	203	209	PF00069	0.352
MOD_Plk_4	111	117	PF00069	0.435
MOD_Plk_4	132	138	PF00069	0.354
MOD_Plk_4	27	33	PF00069	0.681
TRG_DiLeu_BaEn_1	82	87	PF01217	0.371
TRG_ENDOCYTIC_2	140	143	PF00928	0.290
TRG_ENDOCYTIC_2	144	147	PF00928	0.290
TRG_ER_diArg_1	153	155	PF00400	0.451
TRG_NES_CRM1_1	192	204	PF08389	0.429
TRG_Pf-PMV_PEXEL_1	191	196	PF00026	0.349

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I8Z2	Leptomonas seymouri	49%	98%
A0A0S4IZZ0	Bodo saltans	25%	100%
A0A0S4KLZ2	Bodo saltans	38%	81%
A0A1X0PA72	Trypanosomatidae	32%	96%
A0A3R7K285	Trypanosoma rangeli	35%	86%
A0A3S7X8J3	Leishmania donovani	89%	100%
A4HBF0	Leishmania braziliensis	69%	100%
A4IAJ8	Leishmania infantum	89%	100%
C9ZLP3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
Q12395	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	88%
Q4Q2B4	Leishmania major	86%	100%
Q54GP1	Dictyostelium discoideum	22%	95%
Q6C0B6	Yarrowia lipolytica (strain CLIB 122 / E 150)	22%	98%
Q9U3C8	Caenorhabditis elegans	21%	80%
Q9VUQ8	Drosophila melanogaster	24%	82%
V5BLR0	Trypanosoma cruzi	34%	94%