Asparagine--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

asparaginyl-tRNA synthetase, putative

Species:

Leishmania mexicana

UniProt:

E9B520_LEIMU

TriTrypDb:

LmxM.33.2340

Length:

884

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

E9B520

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B520

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006399	tRNA metabolic process	7	12
GO:0006418	tRNA aminoacylation for protein translation	6	12
GO:0006421	asparaginyl-tRNA aminoacylation	7	10
GO:0006520	amino acid metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0019752	carboxylic acid metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043038	amino acid activation	4	12
GO:0043039	tRNA aminoacylation	5	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043436	oxoacid metabolic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044281	small molecule metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901564	organonitrogen compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003676	nucleic acid binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004812	aminoacyl-tRNA ligase activity	4	12
GO:0004816	asparagine-tRNA ligase activity	5	10
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	820	824	PF00656	0.588
CLV_C14_Caspase3-7	828	832	PF00656	0.495
CLV_C14_Caspase3-7	868	872	PF00656	0.512
CLV_NRD_NRD_1	167	169	PF00675	0.751
CLV_NRD_NRD_1	210	212	PF00675	0.700
CLV_NRD_NRD_1	374	376	PF00675	0.373
CLV_NRD_NRD_1	724	726	PF00675	0.297
CLV_NRD_NRD_1	842	844	PF00675	0.297
CLV_PCSK_FUR_1	165	169	PF00082	0.766
CLV_PCSK_KEX2_1	167	169	PF00082	0.751
CLV_PCSK_KEX2_1	374	376	PF00082	0.311
CLV_PCSK_KEX2_1	650	652	PF00082	0.286
CLV_PCSK_KEX2_1	720	722	PF00082	0.297
CLV_PCSK_KEX2_1	724	726	PF00082	0.276
CLV_PCSK_KEX2_1	826	828	PF00082	0.343
CLV_PCSK_KEX2_1	842	844	PF00082	0.276
CLV_PCSK_PC1ET2_1	650	652	PF00082	0.347
CLV_PCSK_PC1ET2_1	720	722	PF00082	0.372
CLV_PCSK_PC1ET2_1	826	828	PF00082	0.373
CLV_PCSK_PC7_1	646	652	PF00082	0.335
CLV_PCSK_SKI1_1	240	244	PF00082	0.311
CLV_PCSK_SKI1_1	273	277	PF00082	0.297
CLV_PCSK_SKI1_1	388	392	PF00082	0.442
CLV_PCSK_SKI1_1	84	88	PF00082	0.764
CLV_Separin_Metazoa	572	576	PF03568	0.527
DEG_APCC_DBOX_1	569	577	PF00400	0.535
DEG_SCF_FBW7_1	184	191	PF00400	0.681
DEG_SPOP_SBC_1	121	125	PF00917	0.653
DOC_CKS1_1	185	190	PF01111	0.683
DOC_CYCLIN_yCln2_LP_2	157	163	PF00134	0.662
DOC_CYCLIN_yCln2_LP_2	516	522	PF00134	0.497
DOC_CYCLIN_yCln2_LP_2	54	60	PF00134	0.616
DOC_MAPK_gen_1	248	258	PF00069	0.496
DOC_MAPK_gen_1	361	370	PF00069	0.573
DOC_MAPK_gen_1	464	473	PF00069	0.494
DOC_MAPK_gen_1	568	576	PF00069	0.604
DOC_MAPK_gen_1	724	734	PF00069	0.486
DOC_MAPK_MEF2A_6	151	159	PF00069	0.646
DOC_MAPK_RevD_3	153	168	PF00069	0.719
DOC_PP1_RVXF_1	856	863	PF00149	0.486
DOC_PP2B_LxvP_1	157	160	PF13499	0.709
DOC_PP2B_LxvP_1	516	519	PF13499	0.497
DOC_USP7_MATH_1	104	108	PF00917	0.622
DOC_USP7_MATH_1	121	125	PF00917	0.524
DOC_USP7_MATH_1	131	135	PF00917	0.736
DOC_USP7_MATH_1	204	208	PF00917	0.769
DOC_USP7_MATH_1	291	295	PF00917	0.445
DOC_USP7_MATH_1	316	320	PF00917	0.585
DOC_USP7_MATH_1	325	329	PF00917	0.529
DOC_USP7_MATH_1	543	547	PF00917	0.542
DOC_USP7_MATH_1	644	648	PF00917	0.495
DOC_USP7_UBL2_3	208	212	PF12436	0.510
DOC_USP7_UBL2_3	716	720	PF12436	0.495
DOC_WW_Pin1_4	177	182	PF00397	0.717
DOC_WW_Pin1_4	184	189	PF00397	0.699
DOC_WW_Pin1_4	265	270	PF00397	0.491
DOC_WW_Pin1_4	439	444	PF00397	0.551
DOC_WW_Pin1_4	53	58	PF00397	0.613
DOC_WW_Pin1_4	587	592	PF00397	0.497
DOC_WW_Pin1_4	715	720	PF00397	0.564
DOC_WW_Pin1_4	74	79	PF00397	0.770
LIG_14-3-3_CanoR_1	118	127	PF00244	0.671
LIG_14-3-3_CanoR_1	140	149	PF00244	0.714
LIG_14-3-3_CanoR_1	165	171	PF00244	0.681
LIG_14-3-3_CanoR_1	211	218	PF00244	0.681
LIG_14-3-3_CanoR_1	470	474	PF00244	0.497
LIG_14-3-3_CanoR_1	651	659	PF00244	0.488
LIG_14-3-3_CanoR_1	8	14	PF00244	0.656
LIG_14-3-3_CanoR_1	84	93	PF00244	0.758
LIG_Actin_WH2_2	366	382	PF00022	0.547
LIG_APCC_ABBA_1	574	579	PF00400	0.535
LIG_APCC_ABBAyCdc20_2	284	290	PF00400	0.392
LIG_BRCT_BRCA1_1	602	606	PF00533	0.486
LIG_deltaCOP1_diTrp_1	15	21	PF00928	0.417
LIG_eIF4E_1	816	822	PF01652	0.573
LIG_FHA_1	189	195	PF00498	0.662
LIG_FHA_1	223	229	PF00498	0.546
LIG_FHA_1	291	297	PF00498	0.525
LIG_FHA_1	302	308	PF00498	0.452
LIG_FHA_1	478	484	PF00498	0.564
LIG_FHA_1	627	633	PF00498	0.507
LIG_FHA_1	793	799	PF00498	0.486
LIG_FHA_2	245	251	PF00498	0.392
LIG_FHA_2	358	364	PF00498	0.589
LIG_FHA_2	595	601	PF00498	0.494
LIG_FHA_2	602	608	PF00498	0.476
LIG_FHA_2	655	661	PF00498	0.497
LIG_FHA_2	683	689	PF00498	0.585
LIG_FHA_2	791	797	PF00498	0.498
LIG_GBD_Chelix_1	192	200	PF00786	0.579
LIG_LIR_Gen_1	15	24	PF02991	0.418
LIG_LIR_Gen_1	472	478	PF02991	0.490
LIG_LIR_Gen_1	526	534	PF02991	0.549
LIG_LIR_Gen_1	603	611	PF02991	0.478
LIG_LIR_Gen_1	654	663	PF02991	0.588
LIG_LIR_Gen_1	667	675	PF02991	0.486
LIG_LIR_Gen_1	769	778	PF02991	0.573
LIG_LIR_Nem_3	15	20	PF02991	0.416
LIG_LIR_Nem_3	430	435	PF02991	0.459
LIG_LIR_Nem_3	472	476	PF02991	0.490
LIG_LIR_Nem_3	526	530	PF02991	0.539
LIG_LIR_Nem_3	583	589	PF02991	0.579
LIG_LIR_Nem_3	603	609	PF02991	0.356
LIG_LIR_Nem_3	654	659	PF02991	0.590
LIG_LIR_Nem_3	667	671	PF02991	0.422
LIG_LIR_Nem_3	833	838	PF02991	0.505
LIG_MYND_1	78	82	PF01753	0.679
LIG_Pex14_1	582	586	PF04695	0.573
LIG_Pex14_2	577	581	PF04695	0.497
LIG_Pex14_2	772	776	PF04695	0.527
LIG_Rb_pABgroove_1	545	553	PF01858	0.459
LIG_RPA_C_Fungi	334	346	PF08784	0.406
LIG_SH2_CRK	527	531	PF00017	0.417
LIG_SH2_NCK_1	527	531	PF00017	0.417
LIG_SH2_SRC	624	627	PF00017	0.448
LIG_SH2_SRC	728	731	PF00017	0.395
LIG_SH2_STAP1	728	732	PF00017	0.373
LIG_SH2_STAT3	616	619	PF00017	0.373
LIG_SH2_STAT3	838	841	PF00017	0.338
LIG_SH2_STAT5	457	460	PF00017	0.423
LIG_SH2_STAT5	616	619	PF00017	0.339
LIG_SH2_STAT5	624	627	PF00017	0.315
LIG_SH2_STAT5	636	639	PF00017	0.156
LIG_SH2_STAT5	838	841	PF00017	0.338
LIG_SH3_1	716	722	PF00018	0.345
LIG_SH3_2	719	724	PF14604	0.338
LIG_SH3_2	79	84	PF14604	0.759
LIG_SH3_3	178	184	PF00018	0.763
LIG_SH3_3	280	286	PF00018	0.427
LIG_SH3_3	586	592	PF00018	0.338
LIG_SH3_3	706	712	PF00018	0.327
LIG_SH3_3	716	722	PF00018	0.356
LIG_SH3_3	76	82	PF00018	0.764
LIG_SH3_3	786	792	PF00018	0.376
LIG_SH3_3	797	803	PF00018	0.323
LIG_SH3_3	90	96	PF00018	0.513
LIG_SH3_3	98	104	PF00018	0.587
LIG_SUMO_SIM_anti_2	795	802	PF11976	0.338
LIG_SUMO_SIM_par_1	95	100	PF11976	0.687
LIG_TRAF2_1	269	272	PF00917	0.206
LIG_TRAF2_1	411	414	PF00917	0.783
LIG_TRAF2_2	699	704	PF00917	0.406
LIG_UBA3_1	605	614	PF00899	0.300
LIG_UBA3_1	863	870	PF00899	0.459
LIG_WRC_WIRS_1	292	297	PF05994	0.356
LIG_WRC_WIRS_1	665	670	PF05994	0.459
LIG_WRC_WIRS_1	780	785	PF05994	0.338
MOD_CDK_SPK_2	177	182	PF00069	0.776
MOD_CDK_SPK_2	265	270	PF00069	0.338
MOD_CDK_SPK_2	715	720	PF00069	0.406
MOD_CDK_SPxK_1	184	190	PF00069	0.725
MOD_CDK_SPxK_1	715	721	PF00069	0.459
MOD_CK1_1	122	128	PF00069	0.748
MOD_CK1_1	134	140	PF00069	0.734
MOD_CK1_1	144	150	PF00069	0.667
MOD_CK1_1	469	475	PF00069	0.354
MOD_CK1_1	533	539	PF00069	0.477
MOD_CK1_1	654	660	PF00069	0.338
MOD_CK1_1	779	785	PF00069	0.406
MOD_CK2_1	244	250	PF00069	0.331
MOD_CK2_1	265	271	PF00069	0.319
MOD_CK2_1	446	452	PF00069	0.482
MOD_CK2_1	594	600	PF00069	0.373
MOD_CK2_1	601	607	PF00069	0.373
MOD_CK2_1	654	660	PF00069	0.338
MOD_CK2_1	682	688	PF00069	0.406
MOD_CK2_1	790	796	PF00069	0.344
MOD_GlcNHglycan	124	127	PF01048	0.759
MOD_GlcNHglycan	327	330	PF01048	0.413
MOD_GSK3_1	116	123	PF00069	0.772
MOD_GSK3_1	130	137	PF00069	0.692
MOD_GSK3_1	141	148	PF00069	0.667
MOD_GSK3_1	184	191	PF00069	0.653
MOD_GSK3_1	321	328	PF00069	0.355
MOD_GSK3_1	539	546	PF00069	0.450
MOD_GSK3_1	558	565	PF00069	0.239
MOD_GSK3_1	590	597	PF00069	0.384
MOD_GSK3_1	624	631	PF00069	0.391
MOD_GSK3_1	678	685	PF00069	0.423
MOD_GSK3_1	69	76	PF00069	0.788
MOD_GSK3_1	790	797	PF00069	0.375
MOD_GSK3_1	84	91	PF00069	0.704
MOD_GSK3_1	9	16	PF00069	0.444
MOD_NEK2_1	130	135	PF00069	0.767
MOD_NEK2_1	301	306	PF00069	0.354
MOD_NEK2_1	35	40	PF00069	0.731
MOD_NEK2_1	562	567	PF00069	0.392
MOD_NEK2_1	58	63	PF00069	0.730
MOD_NEK2_1	594	599	PF00069	0.360
MOD_NEK2_1	617	622	PF00069	0.338
MOD_NEK2_1	678	683	PF00069	0.406
MOD_NEK2_1	73	78	PF00069	0.643
MOD_NEK2_1	809	814	PF00069	0.503
MOD_NEK2_1	830	835	PF00069	0.463
MOD_NEK2_1	89	94	PF00069	0.719
MOD_NEK2_1	9	14	PF00069	0.652
MOD_NEK2_2	601	606	PF00069	0.338
MOD_PIKK_1	301	307	PF00454	0.338
MOD_PIKK_1	594	600	PF00454	0.373
MOD_PIKK_1	615	621	PF00454	0.338
MOD_PIKK_1	697	703	PF00454	0.481
MOD_PKA_1	211	217	PF00069	0.607
MOD_PKA_2	166	172	PF00069	0.632
MOD_PKA_2	469	475	PF00069	0.354
MOD_PKA_2	495	501	PF00069	0.338
MOD_PKA_2	502	508	PF00069	0.338
MOD_PKA_2	558	564	PF00069	0.326
MOD_PKA_2	9	15	PF00069	0.594
MOD_PKB_1	464	472	PF00069	0.338
MOD_Plk_1	543	549	PF00069	0.568
MOD_Plk_1	654	660	PF00069	0.338
MOD_Plk_1	830	836	PF00069	0.481
MOD_Plk_2-3	664	670	PF00069	0.433
MOD_Plk_2-3	790	796	PF00069	0.375
MOD_Plk_4	195	201	PF00069	0.695
MOD_Plk_4	291	297	PF00069	0.436
MOD_Plk_4	469	475	PF00069	0.453
MOD_Plk_4	536	542	PF00069	0.546
MOD_Plk_4	562	568	PF00069	0.423
MOD_Plk_4	590	596	PF00069	0.338
MOD_Plk_4	601	607	PF00069	0.338
MOD_Plk_4	678	684	PF00069	0.410
MOD_Plk_4	779	785	PF00069	0.338
MOD_Plk_4	831	837	PF00069	0.356
MOD_Plk_4	9	15	PF00069	0.612
MOD_ProDKin_1	177	183	PF00069	0.715
MOD_ProDKin_1	184	190	PF00069	0.701
MOD_ProDKin_1	265	271	PF00069	0.345
MOD_ProDKin_1	439	445	PF00069	0.554
MOD_ProDKin_1	53	59	PF00069	0.614
MOD_ProDKin_1	587	593	PF00069	0.354
MOD_ProDKin_1	715	721	PF00069	0.448
MOD_ProDKin_1	74	80	PF00069	0.772
MOD_SUMO_for_1	411	414	PF00179	0.734
MOD_SUMO_for_1	419	422	PF00179	0.732
MOD_SUMO_for_1	512	515	PF00179	0.384
MOD_SUMO_rev_2	205	210	PF00179	0.495
MOD_SUMO_rev_2	265	275	PF00179	0.345
MOD_SUMO_rev_2	294	301	PF00179	0.206
MOD_SUMO_rev_2	401	409	PF00179	0.642
TRG_DiLeu_BaLyEn_6	855	860	PF01217	0.395
TRG_ENDOCYTIC_2	527	530	PF00928	0.412
TRG_ENDOCYTIC_2	586	589	PF00928	0.438
TRG_ENDOCYTIC_2	636	639	PF00928	0.338
TRG_ER_diArg_1	110	113	PF00400	0.712
TRG_ER_diArg_1	115	118	PF00400	0.627
TRG_ER_diArg_1	164	167	PF00400	0.753
TRG_ER_diArg_1	373	375	PF00400	0.459
TRG_ER_diArg_1	723	725	PF00400	0.339
TRG_ER_diArg_1	8	11	PF00400	0.641
TRG_ER_diArg_1	842	844	PF00400	0.453
TRG_Pf-PMV_PEXEL_1	651	655	PF00026	0.338

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMR3	Leptomonas seymouri	78%	100%
A0A0S4JDH2	Bodo saltans	60%	100%
A0A1X0P9Y4	Trypanosomatidae	65%	100%
A0A3R7P1H1	Trypanosoma rangeli	63%	100%
A0A3S7X7X7	Leishmania donovani	92%	99%
A4HAU6	Leishmania braziliensis	77%	100%
A4IA13	Leishmania infantum	92%	99%
C9ZMM2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	100%
E9AV72	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
Q4Q2W6	Leishmania major	90%	100%
Q554D9	Dictyostelium discoideum	39%	100%
V5DGS5	Trypanosoma cruzi	63%	100%