Putative ubiquitin-conjugating enzyme

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2, putative

Species:

Leishmania mexicana

UniProt:

E9B4Y0_LEIMU

TriTrypDb:

LmxM.33.1555

Length:

166

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B4Y0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B4Y0

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	3
GO:0004842	ubiquitin-protein transferase activity	4	1
GO:0016740	transferase activity	2	1
GO:0016874	ligase activity	2	3
GO:0019787	ubiquitin-like protein transferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	7	9	PF00675	0.539
CLV_PCSK_KEX2_1	7	9	PF00082	0.493
CLV_PCSK_SKI1_1	124	128	PF00082	0.331
CLV_PCSK_SKI1_1	83	87	PF00082	0.273
DOC_CKS1_1	111	116	PF01111	0.344
DOC_MAPK_gen_1	4	13	PF00069	0.541
DOC_PP4_FxxP_1	77	80	PF00568	0.262
DOC_USP7_MATH_1	54	58	PF00917	0.366
DOC_USP7_MATH_1	91	95	PF00917	0.283
DOC_WW_Pin1_4	110	115	PF00397	0.344
DOC_WW_Pin1_4	145	150	PF00397	0.344
DOC_WW_Pin1_4	21	26	PF00397	0.507
LIG_BIR_II_1	1	5	PF00653	0.498
LIG_FHA_1	110	116	PF00498	0.444
LIG_FHA_1	130	136	PF00498	0.349
LIG_FHA_1	61	67	PF00498	0.335
LIG_FHA_2	146	152	PF00498	0.331
LIG_FHA_2	60	66	PF00498	0.344
LIG_LIR_Apic_2	74	80	PF02991	0.303
LIG_LIR_Gen_1	12	20	PF02991	0.561
LIG_LIR_Gen_1	132	141	PF02991	0.349
LIG_LIR_Gen_1	62	70	PF02991	0.383
LIG_LIR_Nem_3	12	16	PF02991	0.557
LIG_LIR_Nem_3	129	133	PF02991	0.294
LIG_LIR_Nem_3	62	67	PF02991	0.383
LIG_MAD2	83	91	PF02301	0.332
LIG_SH2_CRK	133	137	PF00017	0.344
LIG_SH2_CRK	64	68	PF00017	0.341
LIG_SH2_STAP1	64	68	PF00017	0.332
LIG_SH3_3	108	114	PF00018	0.332
LIG_SH3_3	48	54	PF00018	0.278
LIG_SH3_3	73	79	PF00018	0.307
LIG_TRAF2_1	148	151	PF00917	0.332
MOD_CK1_1	2	8	PF00069	0.574
MOD_CK2_1	145	151	PF00069	0.331
MOD_GlcNHglycan	21	24	PF01048	0.501
MOD_GlcNHglycan	27	30	PF01048	0.549
MOD_GSK3_1	19	26	PF00069	0.628
MOD_N-GLC_1	136	141	PF02516	0.293
MOD_NEK2_1	136	141	PF00069	0.267
MOD_NEK2_1	19	24	PF00069	0.551
MOD_NEK2_2	54	59	PF00069	0.332
MOD_PIKK_1	68	74	PF00454	0.216
MOD_Plk_4	54	60	PF00069	0.345
MOD_ProDKin_1	110	116	PF00069	0.344
MOD_ProDKin_1	145	151	PF00069	0.344
MOD_ProDKin_1	21	27	PF00069	0.511
MOD_SUMO_rev_2	107	112	PF00179	0.373
TRG_ENDOCYTIC_2	133	136	PF00928	0.344
TRG_ENDOCYTIC_2	64	67	PF00928	0.341
TRG_ER_diArg_1	47	50	PF00400	0.391
TRG_ER_diArg_1	6	8	PF00400	0.537

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I707	Leptomonas seymouri	28%	100%
A0A0N1IA94	Leptomonas seymouri	58%	100%
A0A0S4IWQ9	Bodo saltans	33%	86%
A0A0S4JGV6	Bodo saltans	25%	100%
A0A0S4JK56	Bodo saltans	26%	100%
A0A0S4KIJ1	Bodo saltans	26%	92%
A0A0S4KJ33	Bodo saltans	25%	100%
A0A1X0NH68	Trypanosomatidae	28%	100%
A0A1X0NT81	Trypanosomatidae	30%	79%
A0A1X0NW06	Trypanosomatidae	31%	100%
A0A1X0P9M3	Trypanosomatidae	30%	100%
A0A3Q8II37	Leishmania donovani	97%	100%
A0A3Q8II46	Leishmania donovani	25%	100%
A0A422MVJ2	Trypanosoma rangeli	26%	100%
A0A422MW82	Trypanosoma rangeli	30%	100%
A0A422NDI3	Trypanosoma rangeli	31%	78%
A0A422NWQ7	Trypanosoma rangeli	28%	100%
A3KN22	Bos taurus	28%	91%
A4HAM6	Leishmania braziliensis	82%	100%
A4HMF2	Leishmania braziliensis	25%	100%
A4I9W7	Leishmania infantum	97%	100%
A4IB24	Leishmania infantum	25%	100%
A5PKP9	Xenopus laevis	25%	100%
C9ZMR5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	100%
C9ZNM0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
C9ZW85	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
E9B609	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
O14933	Homo sapiens	23%	100%
O74196	Colletotrichum gloeosporioides	32%	100%
O74201	Candida albicans (strain SC5314 / ATCC MYA-2876)	27%	93%
P06104	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	30%	97%
P0CS16	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	26%	98%
P0CS17	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	26%	98%
P23566	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
P25153	Drosophila melanogaster	29%	100%
P25865	Arabidopsis thaliana	28%	100%
P25866	Triticum aestivum	29%	100%
P25868	Triticum aestivum	25%	99%
P35130	Medicago sativa	28%	100%
P35131	Arabidopsis thaliana	30%	100%
P35132	Arabidopsis thaliana	28%	100%
P35133	Arabidopsis thaliana	29%	100%
P35135	Solanum lycopersicum	28%	100%
P42745	Arabidopsis thaliana	28%	100%
P42746	Arabidopsis thaliana	27%	100%
P42747	Arabidopsis thaliana	27%	99%
P49459	Homo sapiens	26%	100%
P52478	Caenorhabditis elegans	27%	86%
P52493	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	27%	100%
P61081	Homo sapiens	28%	91%
P61082	Mus musculus	28%	91%
P62253	Homo sapiens	26%	98%
P62254	Mus musculus	26%	98%
P62255	Rattus norvegicus	26%	98%
P63146	Homo sapiens	28%	100%
P63147	Mus musculus	28%	100%
P63148	Oryctolagus cuniculus	28%	100%
P63149	Rattus norvegicus	28%	100%
P78717	Fusarium solani	27%	100%
Q21633	Caenorhabditis elegans	29%	100%
Q32P99	Bos taurus	28%	100%
Q42540	Arabidopsis thaliana	27%	100%
Q42541	Arabidopsis thaliana	27%	100%
Q4PFA5	Ustilago maydis (strain 521 / FGSC 9021)	29%	90%
Q4Q346	Leishmania major	93%	100%
Q4R5Y8	Macaca fascicularis	26%	98%
Q4WLA7	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	26%	100%
Q553F3	Dictyostelium discoideum	30%	100%
Q58FS2	Trichoderma harzianum	25%	100%
Q6BU36	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	26%	99%
Q6C093	Yarrowia lipolytica (strain CLIB 122 / E 150)	28%	100%
Q6C9W0	Yarrowia lipolytica (strain CLIB 122 / E 150)	33%	93%
Q6CUD9	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	30%	100%
Q6DCZ9	Xenopus laevis	27%	91%
Q6FR76	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	30%	99%
Q6P8D9	Xenopus tropicalis	27%	91%
Q75EB8	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	30%	98%
Q8L7T3	Arabidopsis thaliana	28%	92%
Q8S919	Oryza sativa subsp. japonica	28%	100%
Q94F47	Arabidopsis thaliana	29%	100%
Q95044	Spisula solidissima	28%	94%
Q96UP5	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	26%	100%
Q9GNC1	Leishmania major	25%	100%
Q9LJZ5	Arabidopsis thaliana	28%	92%
Q9P6I1	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	100%
Q9SDY5	Arabidopsis thaliana	28%	90%
Q9VSF3	Drosophila melanogaster	26%	92%
Q9VTY6	Drosophila melanogaster	30%	93%
Q9Z255	Mus musculus	26%	100%
V5BVS1	Trypanosoma cruzi	31%	74%