EAP30 family protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

EAP30/Vps36 family, putative

Species:

Leishmania mexicana

UniProt:

E9B4X2_LEIMU

TriTrypDb:

LmxM.33.1470

Length:

396

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000814	ESCRT II complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0036452	ESCRT complex	2	12
GO:0098796	membrane protein complex	2	12
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B4X2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B4X2

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	12
GO:0016192	vesicle-mediated transport	4	12
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0071985	multivesicular body sorting pathway	5	12
GO:0006886	intracellular protein transport	4	1
GO:0007034	vacuolar transport	4	1
GO:0008104	protein localization	4	1
GO:0009987	cellular process	1	1
GO:0015031	protein transport	4	1
GO:0016197	endosomal transport	4	1
GO:0032509	endosome transport via multivesicular body sorting pathway	5	1
GO:0032511	late endosome to vacuole transport via multivesicular body sorting pathway	6	1
GO:0033036	macromolecule localization	2	1
GO:0033365	protein localization to organelle	5	1
GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	5	1
GO:0045184	establishment of protein localization	3	1
GO:0045324	late endosome to vacuole transport	5	1
GO:0046907	intracellular transport	3	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0070727	cellular macromolecule localization	3	1
GO:0071702	organic substance transport	4	1
GO:0071705	nitrogen compound transport	4	1
GO:0072594	establishment of protein localization to organelle	4	1
GO:0072665	protein localization to vacuole	6	1
GO:0072666	establishment of protein localization to vacuole	5	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	204	208	PF00656	0.466
CLV_NRD_NRD_1	110	112	PF00675	0.254
CLV_NRD_NRD_1	163	165	PF00675	0.340
CLV_NRD_NRD_1	240	242	PF00675	0.310
CLV_NRD_NRD_1	3	5	PF00675	0.498
CLV_NRD_NRD_1	370	372	PF00675	0.723
CLV_NRD_NRD_1	381	383	PF00675	0.676
CLV_PCSK_KEX2_1	163	165	PF00082	0.295
CLV_PCSK_KEX2_1	168	170	PF00082	0.259
CLV_PCSK_KEX2_1	2	4	PF00082	0.515
CLV_PCSK_KEX2_1	321	323	PF00082	0.348
CLV_PCSK_KEX2_1	370	372	PF00082	0.658
CLV_PCSK_PC1ET2_1	168	170	PF00082	0.268
CLV_PCSK_PC1ET2_1	321	323	PF00082	0.327
CLV_PCSK_PC1ET2_1	370	372	PF00082	0.637
CLV_PCSK_PC7_1	164	170	PF00082	0.240
CLV_PCSK_PC7_1	317	323	PF00082	0.348
CLV_PCSK_SKI1_1	317	321	PF00082	0.339
CLV_PCSK_SKI1_1	356	360	PF00082	0.670
CLV_PCSK_SKI1_1	83	87	PF00082	0.248
DEG_APCC_DBOX_1	316	324	PF00400	0.539
DEG_Nend_UBRbox_1	1	4	PF02207	0.522
DOC_CKS1_1	203	208	PF01111	0.448
DOC_PP1_RVXF_1	120	126	PF00149	0.539
DOC_USP7_MATH_1	149	153	PF00917	0.590
DOC_USP7_MATH_1	257	261	PF00917	0.550
DOC_USP7_MATH_1	287	291	PF00917	0.470
DOC_USP7_MATH_1	347	351	PF00917	0.701
DOC_USP7_MATH_1	352	356	PF00917	0.736
DOC_USP7_UBL2_3	366	370	PF12436	0.630
DOC_WW_Pin1_4	202	207	PF00397	0.472
DOC_WW_Pin1_4	348	353	PF00397	0.656
LIG_14-3-3_CanoR_1	169	178	PF00244	0.456
LIG_14-3-3_CanoR_1	241	245	PF00244	0.490
LIG_APCC_ABBAyCdc20_2	82	88	PF00400	0.491
LIG_BIR_III_4	88	92	PF00653	0.491
LIG_BRCT_BRCA1_1	289	293	PF00533	0.457
LIG_eIF4E_1	131	137	PF01652	0.491
LIG_FHA_1	326	332	PF00498	0.538
LIG_FHA_1	360	366	PF00498	0.728
LIG_FHA_2	202	208	PF00498	0.448
LIG_FHA_2	244	250	PF00498	0.406
LIG_GBD_Chelix_1	70	78	PF00786	0.304
LIG_LIR_Nem_3	384	390	PF02991	0.448
LIG_PALB2_WD40_1	384	392	PF16756	0.502
LIG_PTB_Apo_2	91	98	PF02174	0.396
LIG_PTB_Phospho_1	91	97	PF10480	0.396
LIG_REV1ctd_RIR_1	123	133	PF16727	0.539
LIG_SH2_CRK	191	195	PF00017	0.445
LIG_SH2_PTP2	117	120	PF00017	0.448
LIG_SH2_STAT5	117	120	PF00017	0.439
LIG_SH2_STAT5	201	204	PF00017	0.437
LIG_SH2_STAT5	220	223	PF00017	0.504
LIG_SUMO_SIM_anti_2	182	188	PF11976	0.539
LIG_SUMO_SIM_anti_2	213	219	PF11976	0.506
LIG_TRAF2_1	236	239	PF00917	0.572
LIG_TRAF2_1	361	364	PF00917	0.607
LIG_TYR_ITIM	385	390	PF00017	0.543
LIG_UBA3_1	177	183	PF00899	0.539
MOD_CDC14_SPxK_1	351	354	PF00782	0.652
MOD_CDK_SPxK_1	348	354	PF00069	0.656
MOD_CK1_1	151	157	PF00069	0.549
MOD_CK1_1	182	188	PF00069	0.518
MOD_CK1_1	243	249	PF00069	0.513
MOD_CK1_1	261	267	PF00069	0.516
MOD_CK1_1	55	61	PF00069	0.491
MOD_CK2_1	243	249	PF00069	0.383
MOD_CK2_1	357	363	PF00069	0.693
MOD_Cter_Amidation	166	169	PF01082	0.276
MOD_GlcNHglycan	151	154	PF01048	0.352
MOD_GlcNHglycan	155	158	PF01048	0.321
MOD_GlcNHglycan	242	245	PF01048	0.312
MOD_GlcNHglycan	263	266	PF01048	0.336
MOD_GlcNHglycan	306	309	PF01048	0.391
MOD_GlcNHglycan	336	340	PF01048	0.674
MOD_GSK3_1	149	156	PF00069	0.569
MOD_GSK3_1	197	204	PF00069	0.435
MOD_GSK3_1	257	264	PF00069	0.548
MOD_GSK3_1	287	294	PF00069	0.521
MOD_GSK3_1	300	307	PF00069	0.491
MOD_GSK3_1	331	338	PF00069	0.614
MOD_GSK3_1	348	355	PF00069	0.750
MOD_N-GLC_1	287	292	PF02516	0.274
MOD_N-GLC_2	159	161	PF02516	0.162
MOD_NEK2_1	291	296	PF00069	0.587
MOD_NEK2_1	70	75	PF00069	0.441
MOD_NEK2_1	78	83	PF00069	0.431
MOD_PIKK_1	291	297	PF00454	0.497
MOD_PIKK_1	300	306	PF00454	0.433
MOD_PIKK_1	331	337	PF00454	0.704
MOD_PKA_1	371	377	PF00069	0.718
MOD_PKA_2	240	246	PF00069	0.447
MOD_PKA_2	252	258	PF00069	0.476
MOD_PKA_2	300	306	PF00069	0.448
MOD_PKA_2	340	346	PF00069	0.795
MOD_PKA_2	347	353	PF00069	0.686
MOD_Plk_1	131	137	PF00069	0.539
MOD_Plk_1	287	293	PF00069	0.448
MOD_Plk_4	132	138	PF00069	0.574
MOD_Plk_4	182	188	PF00069	0.537
MOD_Plk_4	197	203	PF00069	0.497
MOD_Plk_4	325	331	PF00069	0.437
MOD_ProDKin_1	202	208	PF00069	0.472
MOD_ProDKin_1	348	354	PF00069	0.656
MOD_SUMO_rev_2	105	113	PF00179	0.448
MOD_SUMO_rev_2	162	170	PF00179	0.478
MOD_SUMO_rev_2	360	368	PF00179	0.605
MOD_SUMO_rev_2	378	385	PF00179	0.526
TRG_ENDOCYTIC_2	117	120	PF00928	0.448
TRG_ENDOCYTIC_2	191	194	PF00928	0.422
TRG_ENDOCYTIC_2	387	390	PF00928	0.396
TRG_ENDOCYTIC_2	96	99	PF00928	0.448
TRG_ER_diArg_1	1	4	PF00400	0.522
TRG_ER_diArg_1	63	66	PF00400	0.459
TRG_Pf-PMV_PEXEL_1	68	72	PF00026	0.286
TRG_PTS2	1	39	PF00400	0.334

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HSW5	Leptomonas seymouri	72%	100%
A0A0S4J518	Bodo saltans	27%	96%
A0A1X0P9K2	Trypanosomatidae	44%	100%
A0A3Q8IKY6	Leishmania donovani	94%	100%
A0A3R7NWX2	Trypanosoma rangeli	43%	100%
A4H7S7	Leishmania braziliensis	80%	100%
A4I9V9	Leishmania infantum	94%	100%
C9ZMT0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
Q4Q355	Leishmania major	92%	99%
V5BT20	Trypanosoma cruzi	41%	100%