PseudoU_synth_1 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase, putative

Species:

Leishmania mexicana

UniProt:

E9B4J9_LEIMU

TriTrypDb:

LmxM.33.0310

Length:

700

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	4
GO:0043227	membrane-bounded organelle	3	4
GO:0043229	intracellular organelle	3	4
GO:0043231	intracellular membrane-bounded organelle	4	4
GO:0110165	cellular anatomical entity	1	4

Expansion

Sequence features

E9B4J9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B4J9

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	8
GO:0006139	nucleobase-containing compound metabolic process	3	8
GO:0006396	RNA processing	6	8
GO:0006399	tRNA metabolic process	7	8
GO:0006725	cellular aromatic compound metabolic process	3	8
GO:0006807	nitrogen compound metabolic process	2	8
GO:0008033	tRNA processing	8	8
GO:0008152	metabolic process	1	8
GO:0009451	RNA modification	5	8
GO:0009987	cellular process	1	8
GO:0016070	RNA metabolic process	5	8
GO:0034470	ncRNA processing	7	8
GO:0034641	cellular nitrogen compound metabolic process	3	8
GO:0034660	ncRNA metabolic process	6	8
GO:0043170	macromolecule metabolic process	3	8
GO:0043412	macromolecule modification	4	8
GO:0044237	cellular metabolic process	2	8
GO:0044238	primary metabolic process	2	8
GO:0046483	heterocycle metabolic process	3	8
GO:0071704	organic substance metabolic process	2	8
GO:0090304	nucleic acid metabolic process	4	8
GO:1901360	organic cyclic compound metabolic process	3	8
GO:0006400	tRNA modification	6	4
GO:0016071	mRNA metabolic process	6	1
GO:0016556	mRNA modification	6	1
GO:0031119	tRNA pseudouridine synthesis	7	4
GO:1990481	mRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	8
GO:0003723	RNA binding	4	8
GO:0003824	catalytic activity	1	8
GO:0005488	binding	1	8
GO:0009982	pseudouridine synthase activity	4	8
GO:0016853	isomerase activity	2	8
GO:0016866	intramolecular transferase activity	3	8
GO:0097159	organic cyclic compound binding	2	8
GO:1901363	heterocyclic compound binding	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	373	377	PF00656	0.777
CLV_C14_Caspase3-7	520	524	PF00656	0.210
CLV_NRD_NRD_1	219	221	PF00675	0.413
CLV_NRD_NRD_1	282	284	PF00675	0.713
CLV_NRD_NRD_1	289	291	PF00675	0.732
CLV_NRD_NRD_1	298	300	PF00675	0.735
CLV_NRD_NRD_1	438	440	PF00675	0.364
CLV_NRD_NRD_1	53	55	PF00675	0.479
CLV_NRD_NRD_1	577	579	PF00675	0.376
CLV_NRD_NRD_1	602	604	PF00675	0.430
CLV_NRD_NRD_1	607	609	PF00675	0.430
CLV_NRD_NRD_1	630	632	PF00675	0.518
CLV_NRD_NRD_1	664	666	PF00675	0.456
CLV_PCSK_FUR_1	51	55	PF00082	0.563
CLV_PCSK_KEX2_1	187	189	PF00082	0.439
CLV_PCSK_KEX2_1	219	221	PF00082	0.465
CLV_PCSK_KEX2_1	264	266	PF00082	0.735
CLV_PCSK_KEX2_1	281	283	PF00082	0.605
CLV_PCSK_KEX2_1	298	300	PF00082	0.720
CLV_PCSK_KEX2_1	438	440	PF00082	0.364
CLV_PCSK_KEX2_1	50	52	PF00082	0.553
CLV_PCSK_KEX2_1	53	55	PF00082	0.463
CLV_PCSK_KEX2_1	533	535	PF00082	0.326
CLV_PCSK_KEX2_1	607	609	PF00082	0.430
CLV_PCSK_KEX2_1	630	632	PF00082	0.518
CLV_PCSK_KEX2_1	663	665	PF00082	0.457
CLV_PCSK_PC1ET2_1	187	189	PF00082	0.493
CLV_PCSK_PC1ET2_1	264	266	PF00082	0.735
CLV_PCSK_PC1ET2_1	298	300	PF00082	0.793
CLV_PCSK_PC1ET2_1	50	52	PF00082	0.602
CLV_PCSK_PC1ET2_1	533	535	PF00082	0.326
CLV_PCSK_PC7_1	603	609	PF00082	0.430
CLV_PCSK_SKI1_1	187	191	PF00082	0.436
CLV_PCSK_SKI1_1	269	273	PF00082	0.819
CLV_PCSK_SKI1_1	300	304	PF00082	0.777
CLV_PCSK_SKI1_1	438	442	PF00082	0.429
CLV_PCSK_SKI1_1	598	602	PF00082	0.430
CLV_PCSK_SKI1_1	688	692	PF00082	0.587
CLV_PCSK_SKI1_1	89	93	PF00082	0.473
CLV_Separin_Metazoa	561	565	PF03568	0.376
DEG_Nend_Nbox_1	1	3	PF02207	0.569
DEG_ODPH_VHL_1	245	257	PF01847	0.598
DEG_SCF_FBW7_2	319	326	PF00400	0.706
DOC_CKS1_1	22	27	PF01111	0.612
DOC_CYCLIN_RxL_1	106	117	PF00134	0.248
DOC_CYCLIN_RxL_1	435	445	PF00134	0.571
DOC_CYCLIN_RxL_1	86	95	PF00134	0.474
DOC_MAPK_gen_1	50	60	PF00069	0.466
DOC_MAPK_gen_1	663	671	PF00069	0.483
DOC_MAPK_gen_1	685	693	PF00069	0.452
DOC_MAPK_MEF2A_6	127	136	PF00069	0.404
DOC_MAPK_MEF2A_6	53	62	PF00069	0.422
DOC_MAPK_MEF2A_6	663	671	PF00069	0.430
DOC_PP1_RVXF_1	107	114	PF00149	0.341
DOC_PP1_RVXF_1	251	258	PF00149	0.655
DOC_PP1_RVXF_1	690	696	PF00149	0.525
DOC_PP4_FxxP_1	498	501	PF00568	0.430
DOC_USP7_MATH_1	289	293	PF00917	0.706
DOC_USP7_MATH_1	524	528	PF00917	0.424
DOC_WW_Pin1_4	21	26	PF00397	0.760
DOC_WW_Pin1_4	319	324	PF00397	0.759
DOC_WW_Pin1_4	348	353	PF00397	0.737
DOC_WW_Pin1_4	35	40	PF00397	0.526
DOC_WW_Pin1_4	451	456	PF00397	0.376
DOC_WW_Pin1_4	477	482	PF00397	0.430
DOC_WW_Pin1_4	497	502	PF00397	0.171
LIG_14-3-3_CanoR_1	115	123	PF00244	0.454
LIG_14-3-3_CanoR_1	127	133	PF00244	0.402
LIG_14-3-3_CanoR_1	269	274	PF00244	0.737
LIG_14-3-3_CanoR_1	466	472	PF00244	0.373
LIG_14-3-3_CanoR_1	504	510	PF00244	0.430
LIG_Actin_WH2_2	238	255	PF00022	0.700
LIG_Actin_WH2_2	677	694	PF00022	0.566
LIG_BRCT_BRCA1_1	473	477	PF00533	0.376
LIG_Clathr_ClatBox_1	110	114	PF01394	0.509
LIG_deltaCOP1_diTrp_1	491	498	PF00928	0.385
LIG_EH_1	474	478	PF12763	0.210
LIG_eIF4E_1	596	602	PF01652	0.410
LIG_FHA_1	225	231	PF00498	0.405
LIG_FHA_1	312	318	PF00498	0.657
LIG_FHA_1	320	326	PF00498	0.804
LIG_FHA_1	335	341	PF00498	0.568
LIG_FHA_1	516	522	PF00498	0.408
LIG_FHA_1	645	651	PF00498	0.602
LIG_FHA_1	92	98	PF00498	0.470
LIG_FHA_2	11	17	PF00498	0.710
LIG_FHA_2	230	236	PF00498	0.453
LIG_FHA_2	249	255	PF00498	0.426
LIG_FHA_2	270	276	PF00498	0.798
LIG_FHA_2	506	512	PF00498	0.430
LIG_FHA_2	80	86	PF00498	0.448
LIG_GBD_Chelix_1	667	675	PF00786	0.411
LIG_LIR_Apic_2	222	226	PF02991	0.402
LIG_LIR_Gen_1	227	236	PF02991	0.431
LIG_LIR_Gen_1	404	413	PF02991	0.529
LIG_LIR_Gen_1	447	455	PF02991	0.376
LIG_LIR_Gen_1	621	628	PF02991	0.408
LIG_LIR_Gen_1	99	110	PF02991	0.591
LIG_LIR_Nem_3	207	213	PF02991	0.450
LIG_LIR_Nem_3	38	43	PF02991	0.600
LIG_LIR_Nem_3	447	453	PF02991	0.376
LIG_LIR_Nem_3	463	468	PF02991	0.376
LIG_LIR_Nem_3	621	625	PF02991	0.376
LIG_LIR_Nem_3	67	72	PF02991	0.419
LIG_LIR_Nem_3	79	83	PF02991	0.474
LIG_LIR_Nem_3	99	105	PF02991	0.259
LIG_LYPXL_yS_3	80	83	PF13949	0.512
LIG_PCNA_yPIPBox_3	104	118	PF02747	0.515
LIG_PCNA_yPIPBox_3	492	504	PF02747	0.210
LIG_RPA_C_Fungi	660	672	PF08784	0.477
LIG_SH2_CRK	201	205	PF00017	0.474
LIG_SH2_CRK	427	431	PF00017	0.514
LIG_SH2_CRK	465	469	PF00017	0.501
LIG_SH2_CRK	69	73	PF00017	0.423
LIG_SH2_GRB2like	213	216	PF00017	0.506
LIG_SH2_GRB2like	450	453	PF00017	0.376
LIG_SH2_NCK_1	201	205	PF00017	0.398
LIG_SH2_SRC	138	141	PF00017	0.501
LIG_SH2_STAP1	221	225	PF00017	0.402
LIG_SH2_STAP1	236	240	PF00017	0.484
LIG_SH2_STAP1	398	402	PF00017	0.588
LIG_SH2_STAP1	509	513	PF00017	0.430
LIG_SH2_STAT5	142	145	PF00017	0.515
LIG_SH2_STAT5	213	216	PF00017	0.478
LIG_SH2_STAT5	224	227	PF00017	0.363
LIG_SH2_STAT5	240	243	PF00017	0.538
LIG_SH2_STAT5	596	599	PF00017	0.410
LIG_SH2_STAT5	622	625	PF00017	0.430
LIG_SH2_STAT5	64	67	PF00017	0.405
LIG_SH2_STAT5	69	72	PF00017	0.413
LIG_SH3_1	26	32	PF00018	0.731
LIG_SH3_1	608	614	PF00018	0.430
LIG_SH3_3	131	137	PF00018	0.374
LIG_SH3_3	22	28	PF00018	0.713
LIG_SH3_3	244	250	PF00018	0.600
LIG_SH3_3	349	355	PF00018	0.798
LIG_SH3_3	470	476	PF00018	0.376
LIG_SH3_3	608	614	PF00018	0.376
LIG_SH3_3	75	81	PF00018	0.516
LIG_SUMO_SIM_anti_2	131	136	PF11976	0.459
LIG_SUMO_SIM_par_1	342	349	PF11976	0.738
LIG_SUMO_SIM_par_1	357	363	PF11976	0.721
LIG_SUMO_SIM_par_1	646	651	PF11976	0.601
LIG_TRAF2_1	13	16	PF00917	0.746
LIG_TRAF2_1	149	152	PF00917	0.544
LIG_TRAF2_1	182	185	PF00917	0.474
LIG_TRAF2_1	274	277	PF00917	0.792
LIG_TRAF2_1	508	511	PF00917	0.410
LIG_TRAF2_1	82	85	PF00917	0.422
LIG_UBA3_1	573	579	PF00899	0.345
LIG_WRC_WIRS_1	225	230	PF05994	0.405
LIG_WW_3	266	270	PF00397	0.747
MOD_CDK_SPK_2	21	26	PF00069	0.616
MOD_CDK_SPxK_1	35	41	PF00069	0.553
MOD_CDK_SPxxK_3	451	458	PF00069	0.376
MOD_CDK_SPxxK_3	497	504	PF00069	0.430
MOD_CK1_1	248	254	PF00069	0.575
MOD_CK1_1	348	354	PF00069	0.755
MOD_CK1_1	35	41	PF00069	0.412
MOD_CK1_1	374	380	PF00069	0.716
MOD_CK2_1	10	16	PF00069	0.708
MOD_CK2_1	179	185	PF00069	0.528
MOD_CK2_1	229	235	PF00069	0.484
MOD_CK2_1	248	254	PF00069	0.469
MOD_CK2_1	271	277	PF00069	0.801
MOD_CK2_1	329	335	PF00069	0.706
MOD_CK2_1	367	373	PF00069	0.784
MOD_CK2_1	374	380	PF00069	0.684
MOD_CK2_1	497	503	PF00069	0.258
MOD_CK2_1	505	511	PF00069	0.430
MOD_CK2_1	524	530	PF00069	0.430
MOD_CK2_1	79	85	PF00069	0.461
MOD_Cter_Amidation	217	220	PF01082	0.473
MOD_Cter_Amidation	262	265	PF01082	0.724
MOD_Cter_Amidation	531	534	PF01082	0.326
MOD_GlcNHglycan	174	177	PF01048	0.688
MOD_GlcNHglycan	287	290	PF01048	0.806
MOD_GlcNHglycan	3	7	PF01048	0.720
MOD_GlcNHglycan	330	334	PF01048	0.753
MOD_GlcNHglycan	34	37	PF01048	0.396
MOD_GlcNHglycan	538	541	PF01048	0.513
MOD_GlcNHglycan	624	628	PF01048	0.466
MOD_GSK3_1	236	243	PF00069	0.559
MOD_GSK3_1	285	292	PF00069	0.691
MOD_GSK3_1	329	336	PF00069	0.745
MOD_GSK3_1	367	374	PF00069	0.752
MOD_GSK3_1	456	463	PF00069	0.378
MOD_GSK3_1	467	474	PF00069	0.371
MOD_GSK3_1	511	518	PF00069	0.504
MOD_GSK3_1	91	98	PF00069	0.469
MOD_N-GLC_1	100	105	PF02516	0.511
MOD_N-GLC_1	451	456	PF02516	0.376
MOD_N-GLC_1	491	496	PF02516	0.210
MOD_NEK2_1	345	350	PF00069	0.612
MOD_NEK2_1	406	411	PF00069	0.614
MOD_NEK2_1	412	417	PF00069	0.654
MOD_NEK2_1	467	472	PF00069	0.410
MOD_NEK2_1	618	623	PF00069	0.376
MOD_NEK2_2	460	465	PF00069	0.430
MOD_NEK2_2	683	688	PF00069	0.433
MOD_NEK2_2	695	700	PF00069	0.515
MOD_PIKK_1	245	251	PF00454	0.605
MOD_PIKK_1	346	352	PF00454	0.590
MOD_PIKK_1	371	377	PF00454	0.810
MOD_PKA_2	114	120	PF00069	0.455
MOD_PKA_2	289	295	PF00069	0.728
MOD_Plk_1	460	466	PF00069	0.501
MOD_Plk_1	491	497	PF00069	0.210
MOD_Plk_2-3	511	517	PF00069	0.501
MOD_Plk_2-3	79	85	PF00069	0.501
MOD_Plk_4	236	242	PF00069	0.551
MOD_Plk_4	354	360	PF00069	0.525
MOD_Plk_4	406	412	PF00069	0.571
MOD_Plk_4	568	574	PF00069	0.347
MOD_Plk_4	592	598	PF00069	0.372
MOD_Plk_4	618	624	PF00069	0.376
MOD_ProDKin_1	21	27	PF00069	0.755
MOD_ProDKin_1	319	325	PF00069	0.763
MOD_ProDKin_1	348	354	PF00069	0.735
MOD_ProDKin_1	35	41	PF00069	0.523
MOD_ProDKin_1	451	457	PF00069	0.376
MOD_ProDKin_1	477	483	PF00069	0.430
MOD_ProDKin_1	497	503	PF00069	0.171
MOD_SUMO_rev_2	15	20	PF00179	0.795
MOD_SUMO_rev_2	182	189	PF00179	0.496
MOD_SUMO_rev_2	362	368	PF00179	0.590
TRG_DiLeu_BaEn_1	185	190	PF01217	0.430
TRG_DiLeu_LyEn_5	185	190	PF01217	0.430
TRG_ENDOCYTIC_2	201	204	PF00928	0.417
TRG_ENDOCYTIC_2	42	45	PF00928	0.511
TRG_ENDOCYTIC_2	450	453	PF00928	0.376
TRG_ENDOCYTIC_2	465	468	PF00928	0.376
TRG_ENDOCYTIC_2	622	625	PF00928	0.430
TRG_ENDOCYTIC_2	69	72	PF00928	0.413
TRG_ENDOCYTIC_2	80	83	PF00928	0.458
TRG_ER_diArg_1	219	221	PF00400	0.465
TRG_ER_diArg_1	281	283	PF00400	0.695
TRG_ER_diArg_1	437	439	PF00400	0.365
TRG_ER_diArg_1	51	54	PF00400	0.568
TRG_ER_diArg_1	606	608	PF00400	0.430
TRG_ER_diArg_1	630	632	PF00400	0.518
TRG_ER_diArg_1	663	665	PF00400	0.514
TRG_NLS_Bipartite_1	281	302	PF00514	0.731
TRG_NLS_MonoExtC_3	49	54	PF00514	0.565
TRG_NLS_MonoExtN_4	281	287	PF00514	0.723
TRG_NLS_MonoExtN_4	297	302	PF00514	0.715
TRG_NLS_MonoExtN_4	51	58	PF00514	0.479
TRG_Pf-PMV_PEXEL_1	188	192	PF00026	0.484
TRG_Pf-PMV_PEXEL_1	438	443	PF00026	0.575
TRG_Pf-PMV_PEXEL_1	89	93	PF00026	0.473

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I609	Leptomonas seymouri	68%	100%
A0A3Q8IKM7	Leishmania donovani	93%	100%
A4H7D9	Leishmania braziliensis	80%	100%
A4I9K1	Leishmania infantum	93%	100%
D0A1W0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
Q4Q3H7	Leishmania major	92%	100%