Putative ubiquitin-conjugating enzyme

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme, putative

Species:

Leishmania mexicana

UniProt:

E9B4B7_LEIMU

TriTrypDb:

LmxM.32.2770

Length:

241

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B4B7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B4B7

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006508	proteolysis	4	1
GO:0006511	ubiquitin-dependent protein catabolic process	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009987	cellular process	1	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0019941	modification-dependent protein catabolic process	6	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043632	modification-dependent macromolecule catabolic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0051603	proteolysis involved in protein catabolic process	5	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901575	organic substance catabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	9
GO:0003824	catalytic activity	1	9
GO:0005488	binding	1	9
GO:0005524	ATP binding	5	9
GO:0016740	transferase activity	2	8
GO:0016874	ligase activity	2	4
GO:0017076	purine nucleotide binding	4	9
GO:0030554	adenyl nucleotide binding	5	9
GO:0032553	ribonucleotide binding	3	9
GO:0032555	purine ribonucleotide binding	4	9
GO:0032559	adenyl ribonucleotide binding	5	9
GO:0035639	purine ribonucleoside triphosphate binding	4	9
GO:0036094	small molecule binding	2	9
GO:0043167	ion binding	2	9
GO:0043168	anion binding	3	9
GO:0097159	organic cyclic compound binding	2	9
GO:0097367	carbohydrate derivative binding	2	9
GO:1901265	nucleoside phosphate binding	3	9
GO:1901363	heterocyclic compound binding	2	9
GO:0004842	ubiquitin-protein transferase activity	4	1
GO:0019787	ubiquitin-like protein transferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	139	141	PF00675	0.304
CLV_NRD_NRD_1	210	212	PF00675	0.600
CLV_NRD_NRD_1	239	241	PF00675	0.669
CLV_PCSK_KEX2_1	210	212	PF00082	0.668
CLV_PCSK_KEX2_1	239	241	PF00082	0.590
CLV_PCSK_KEX2_1	94	96	PF00082	0.298
CLV_PCSK_PC1ET2_1	94	96	PF00082	0.298
CLV_PCSK_SKI1_1	104	108	PF00082	0.274
CLV_PCSK_SKI1_1	111	115	PF00082	0.251
CLV_PCSK_SKI1_1	129	133	PF00082	0.351
CLV_PCSK_SKI1_1	210	214	PF00082	0.617
CLV_PCSK_SKI1_1	235	239	PF00082	0.572
CLV_PCSK_SKI1_1	70	74	PF00082	0.264
DEG_APCC_DBOX_1	103	111	PF00400	0.298
DEG_SCF_TRCP1_1	184	190	PF00400	0.529
DOC_CYCLIN_RxL_1	105	116	PF00134	0.264
DOC_CYCLIN_RxL_1	207	216	PF00134	0.612
DOC_MAPK_MEF2A_6	53	60	PF00069	0.358
DOC_MAPK_MEF2A_6	94	103	PF00069	0.278
DOC_PP4_FxxP_1	159	162	PF00568	0.587
DOC_SPAK_OSR1_1	53	57	PF12202	0.358
DOC_USP7_MATH_1	169	173	PF00917	0.640
DOC_USP7_MATH_1	175	179	PF00917	0.673
DOC_USP7_MATH_1	192	196	PF00917	0.751
DOC_USP7_MATH_1	200	204	PF00917	0.649
DOC_USP7_MATH_1	230	234	PF00917	0.638
DOC_USP7_UBL2_3	232	236	PF12436	0.590
DOC_WW_Pin1_4	157	162	PF00397	0.458
DOC_WW_Pin1_4	188	193	PF00397	0.477
DOC_WW_Pin1_4	44	49	PF00397	0.264
DOC_WW_Pin1_4	95	100	PF00397	0.293
LIG_14-3-3_CanoR_1	210	215	PF00244	0.603
LIG_14-3-3_CanoR_1	29	37	PF00244	0.159
LIG_Actin_RPEL_3	88	107	PF02755	0.358
LIG_APCC_ABBA_1	57	62	PF00400	0.233
LIG_EVH1_2	68	72	PF00568	0.358
LIG_FHA_1	207	213	PF00498	0.779
LIG_FHA_1	98	104	PF00498	0.318
LIG_FHA_2	158	164	PF00498	0.649
LIG_FHA_2	211	217	PF00498	0.667
LIG_FHA_2	223	229	PF00498	0.658
LIG_Integrin_RGD_1	85	87	PF01839	0.358
LIG_LIR_Nem_3	133	138	PF02991	0.289
LIG_LIR_Nem_3	61	67	PF02991	0.278
LIG_LYPXL_yS_3	64	67	PF13949	0.264
LIG_REV1ctd_RIR_1	70	79	PF16727	0.358
LIG_SH2_CRK	145	149	PF00017	0.584
LIG_SH2_NCK_1	145	149	PF00017	0.584
LIG_SH2_STAP1	138	142	PF00017	0.367
LIG_SH2_STAT5	145	148	PF00017	0.586
LIG_SH3_2	65	70	PF14604	0.264
LIG_SH3_3	62	68	PF00018	0.264
LIG_SUMO_SIM_par_1	55	62	PF11976	0.321
MOD_CK1_1	171	177	PF00069	0.537
MOD_CK1_1	185	191	PF00069	0.704
MOD_CK1_1	195	201	PF00069	0.745
MOD_CK1_1	203	209	PF00069	0.577
MOD_CK2_1	157	163	PF00069	0.644
MOD_CK2_1	210	216	PF00069	0.661
MOD_CK2_1	222	228	PF00069	0.653
MOD_CK2_1	30	36	PF00069	0.452
MOD_GlcNHglycan	120	123	PF01048	0.264
MOD_GlcNHglycan	176	180	PF01048	0.702
MOD_GlcNHglycan	184	187	PF01048	0.635
MOD_GlcNHglycan	194	197	PF01048	0.630
MOD_GlcNHglycan	202	205	PF01048	0.556
MOD_GlcNHglycan	222	225	PF01048	0.553
MOD_GSK3_1	167	174	PF00069	0.602
MOD_GSK3_1	188	195	PF00069	0.589
MOD_GSK3_1	199	206	PF00069	0.626
MOD_N-GLC_1	200	205	PF02516	0.660
MOD_N-GLC_1	80	85	PF02516	0.332
MOD_NEK2_1	199	204	PF00069	0.595
MOD_NEK2_1	72	77	PF00069	0.315
MOD_PKA_1	210	216	PF00069	0.557
MOD_PKA_2	210	216	PF00069	0.619
MOD_PKA_2	28	34	PF00069	0.324
MOD_ProDKin_1	157	163	PF00069	0.463
MOD_ProDKin_1	188	194	PF00069	0.478
MOD_ProDKin_1	44	50	PF00069	0.264
MOD_ProDKin_1	95	101	PF00069	0.293
MOD_SUMO_for_1	164	167	PF00179	0.529
MOD_SUMO_rev_2	133	143	PF00179	0.358
TRG_DiLeu_BaEn_1	126	131	PF01217	0.264
TRG_DiLeu_LyEn_5	126	131	PF01217	0.264
TRG_ENDOCYTIC_2	145	148	PF00928	0.586
TRG_ENDOCYTIC_2	64	67	PF00928	0.264
TRG_ER_diArg_1	210	212	PF00400	0.554
TRG_ER_diArg_1	238	240	PF00400	0.667
TRG_NLS_MonoExtN_4	232	238	PF00514	0.699
TRG_Pf-PMV_PEXEL_1	111	116	PF00026	0.280
TRG_Pf-PMV_PEXEL_1	129	133	PF00026	0.264
TRG_Pf-PMV_PEXEL_1	9	14	PF00026	0.308

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P683	Leptomonas seymouri	72%	100%
A0A1X0NPI5	Trypanosomatidae	33%	100%
A0A1X0NSR5	Trypanosomatidae	59%	86%
A0A3Q8IFB8	Leishmania donovani	29%	100%
A0A3Q8IMQ9	Leishmania donovani	92%	100%
A0A3S5H604	Leishmania donovani	43%	100%
A4H533	Leishmania braziliensis	40%	100%
A4H861	Leishmania braziliensis	81%	100%
A4HTB1	Leishmania infantum	43%	100%
A4I7L2	Leishmania infantum	29%	100%
A4I9B7	Leishmania infantum	92%	100%
B5DFI8	Rattus norvegicus	42%	100%
E9ALA2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	42%	100%
E9B2J3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
P49427	Homo sapiens	31%	100%
P61085	Bos taurus	27%	100%
P61086	Homo sapiens	27%	100%
P61087	Mus musculus	27%	100%
Q08BH7	Danio rerio	31%	100%
Q1RML1	Bos taurus	37%	100%
Q4Q3Q8	Leishmania major	89%	100%
Q4Q5L3	Leishmania major	29%	100%
Q4QIK2	Leishmania major	40%	100%
Q7ZY08	Xenopus laevis	32%	100%
Q8AVU2	Xenopus laevis	37%	100%
Q8CFI2	Mus musculus	31%	100%
Q9FF66	Arabidopsis thaliana	44%	96%
V5BAJ3	Trypanosoma cruzi	48%	100%