ThiF domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

NEDD8-activating enzyme E1 regulatory subunit, putative

Species:

Leishmania mexicana

UniProt:

E9B4A5_LEIMU

TriTrypDb:

LmxM.32.2650

Length:

789

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B4A5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B4A5

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	5
GO:0008152	metabolic process	1	5
GO:0019538	protein metabolic process	3	5
GO:0032446	protein modification by small protein conjugation	6	5
GO:0036211	protein modification process	4	5
GO:0043170	macromolecule metabolic process	3	5
GO:0043412	macromolecule modification	4	5
GO:0044238	primary metabolic process	2	5
GO:0045116	protein neddylation	7	5
GO:0070647	protein modification by small protein conjugation or removal	5	5
GO:0071704	organic substance metabolic process	2	5
GO:1901564	organonitrogen compound metabolic process	3	5

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	11
GO:0016874	ligase activity	2	11
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	11
GO:0140096	catalytic activity, acting on a protein	2	11
GO:0140657	ATP-dependent activity	1	11
GO:0019781	NEDD8 activating enzyme activity	3	5

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	301	305	PF00656	0.481
CLV_MEL_PAP_1	416	422	PF00089	0.264
CLV_NRD_NRD_1	145	147	PF00675	0.440
CLV_NRD_NRD_1	323	325	PF00675	0.478
CLV_NRD_NRD_1	365	367	PF00675	0.470
CLV_NRD_NRD_1	644	646	PF00675	0.478
CLV_NRD_NRD_1	720	722	PF00675	0.478
CLV_PCSK_KEX2_1	145	147	PF00082	0.440
CLV_PCSK_KEX2_1	323	325	PF00082	0.478
CLV_PCSK_KEX2_1	365	367	PF00082	0.459
CLV_PCSK_KEX2_1	644	646	PF00082	0.478
CLV_PCSK_SKI1_1	411	415	PF00082	0.381
CLV_PCSK_SKI1_1	584	588	PF00082	0.500
CLV_PCSK_SKI1_1	67	71	PF00082	0.440
CLV_PCSK_SKI1_1	721	725	PF00082	0.392
DEG_MDM2_SWIB_1	322	329	PF02201	0.494
DEG_SPOP_SBC_1	182	186	PF00917	0.447
DEG_SPOP_SBC_1	88	92	PF00917	0.173
DOC_CYCLIN_RxL_1	581	591	PF00134	0.491
DOC_CYCLIN_yCln2_LP_2	467	473	PF00134	0.461
DOC_MAPK_DCC_7	232	242	PF00069	0.425
DOC_MAPK_gen_1	433	440	PF00069	0.251
DOC_MAPK_MEF2A_6	232	241	PF00069	0.449
DOC_MAPK_MEF2A_6	738	747	PF00069	0.344
DOC_MAPK_MEF2A_6	764	771	PF00069	0.470
DOC_PP1_RVXF_1	600	607	PF00149	0.480
DOC_PP1_RVXF_1	623	629	PF00149	0.483
DOC_PP1_RVXF_1	643	650	PF00149	0.222
DOC_PP2B_LxvP_1	467	470	PF13499	0.452
DOC_PP2B_LxvP_1	7	10	PF13499	0.415
DOC_USP7_MATH_1	183	187	PF00917	0.543
DOC_USP7_MATH_1	190	194	PF00917	0.755
DOC_USP7_MATH_1	299	303	PF00917	0.426
DOC_USP7_MATH_1	355	359	PF00917	0.458
DOC_USP7_MATH_1	415	419	PF00917	0.424
DOC_USP7_MATH_1	424	428	PF00917	0.580
DOC_USP7_MATH_1	57	61	PF00917	0.464
DOC_USP7_MATH_1	571	575	PF00917	0.374
DOC_USP7_MATH_1	666	670	PF00917	0.540
DOC_USP7_MATH_1	89	93	PF00917	0.346
DOC_USP7_UBL2_3	380	384	PF12436	0.539
DOC_USP7_UBL2_3	553	557	PF12436	0.390
DOC_WW_Pin1_4	194	199	PF00397	0.685
DOC_WW_Pin1_4	202	207	PF00397	0.391
DOC_WW_Pin1_4	217	222	PF00397	0.388
DOC_WW_Pin1_4	418	423	PF00397	0.449
DOC_WW_Pin1_4	440	445	PF00397	0.407
DOC_WW_Pin1_4	92	97	PF00397	0.417
LIG_14-3-3_CanoR_1	324	330	PF00244	0.438
LIG_14-3-3_CanoR_1	337	347	PF00244	0.501
LIG_14-3-3_CanoR_1	354	360	PF00244	0.457
LIG_14-3-3_CanoR_1	365	370	PF00244	0.544
LIG_14-3-3_CanoR_1	602	607	PF00244	0.398
LIG_14-3-3_CanoR_1	631	636	PF00244	0.489
LIG_14-3-3_CanoR_1	67	72	PF00244	0.355
LIG_14-3-3_CanoR_1	75	82	PF00244	0.199
LIG_BIR_II_1	1	5	PF00653	0.609
LIG_BRCT_BRCA1_1	342	346	PF00533	0.584
LIG_deltaCOP1_diTrp_1	596	606	PF00928	0.439
LIG_FHA_1	220	226	PF00498	0.352
LIG_FHA_1	380	386	PF00498	0.541
LIG_FHA_1	458	464	PF00498	0.540
LIG_FHA_1	49	55	PF00498	0.428
LIG_FHA_1	514	520	PF00498	0.509
LIG_FHA_1	589	595	PF00498	0.376
LIG_FHA_1	628	634	PF00498	0.410
LIG_FHA_1	66	72	PF00498	0.458
LIG_FHA_1	752	758	PF00498	0.408
LIG_FHA_1	781	787	PF00498	0.432
LIG_FHA_1	88	94	PF00498	0.404
LIG_FHA_2	326	332	PF00498	0.433
LIG_FHA_2	347	353	PF00498	0.375
LIG_FHA_2	560	566	PF00498	0.533
LIG_FHA_2	591	597	PF00498	0.447
LIG_FHA_2	68	74	PF00498	0.340
LIG_LIR_Gen_1	2	12	PF02991	0.415
LIG_LIR_Nem_3	2	7	PF02991	0.421
LIG_LIR_Nem_3	213	219	PF02991	0.497
LIG_LIR_Nem_3	278	283	PF02991	0.480
LIG_LIR_Nem_3	310	314	PF02991	0.332
LIG_LIR_Nem_3	350	356	PF02991	0.385
LIG_LIR_Nem_3	518	524	PF02991	0.350
LIG_LYPXL_yS_3	314	317	PF13949	0.426
LIG_MYND_2	206	210	PF01753	0.308
LIG_NRBOX	396	402	PF00104	0.435
LIG_PDZ_Class_2	784	789	PF00595	0.494
LIG_Pex14_1	124	128	PF04695	0.349
LIG_Pex14_2	322	326	PF04695	0.475
LIG_PTB_Apo_2	781	788	PF02174	0.491
LIG_SH2_CRK	173	177	PF00017	0.529
LIG_SH2_CRK	353	357	PF00017	0.392
LIG_SH2_CRK	503	507	PF00017	0.428
LIG_SH2_GRB2like	782	785	PF00017	0.498
LIG_SH2_SRC	490	493	PF00017	0.432
LIG_SH2_STAP1	782	786	PF00017	0.497
LIG_SH2_STAT5	254	257	PF00017	0.469
LIG_SH2_STAT5	311	314	PF00017	0.328
LIG_SH2_STAT5	510	513	PF00017	0.361
LIG_SH2_STAT5	653	656	PF00017	0.366
LIG_SH2_STAT5	782	785	PF00017	0.498
LIG_SH2_STAT5	86	89	PF00017	0.402
LIG_SH3_1	153	159	PF00018	0.533
LIG_SH3_1	232	238	PF00018	0.490
LIG_SH3_3	153	159	PF00018	0.574
LIG_SH3_3	200	206	PF00018	0.416
LIG_SH3_3	232	238	PF00018	0.500
LIG_SH3_3	260	266	PF00018	0.561
LIG_SH3_3	293	299	PF00018	0.423
LIG_SH3_3	742	748	PF00018	0.517
LIG_SUMO_SIM_anti_2	208	214	PF11976	0.381
LIG_SUMO_SIM_anti_2	68	74	PF11976	0.355
LIG_SUMO_SIM_par_1	174	180	PF11976	0.377
LIG_SUMO_SIM_par_1	208	214	PF11976	0.328
LIG_SUMO_SIM_par_1	238	245	PF11976	0.450
LIG_SUMO_SIM_par_1	50	56	PF11976	0.398
LIG_SUMO_SIM_par_1	67	74	PF11976	0.192
LIG_TRAF2_1	116	119	PF00917	0.355
LIG_TRAF2_1	330	333	PF00917	0.563
LIG_TRAF2_1	727	730	PF00917	0.476
LIG_TRAF2_1	758	761	PF00917	0.323
LIG_TYR_ITIM	312	317	PF00017	0.333
LIG_TYR_ITIM	519	524	PF00017	0.477
LIG_UBA3_1	345	351	PF00899	0.472
MOD_CK1_1	205	211	PF00069	0.523
MOD_CK1_1	220	226	PF00069	0.348
MOD_CK1_1	340	346	PF00069	0.595
MOD_CK1_1	418	424	PF00069	0.445
MOD_CK1_1	574	580	PF00069	0.558
MOD_CK1_1	605	611	PF00069	0.564
MOD_CK1_1	669	675	PF00069	0.543
MOD_CK1_1	685	691	PF00069	0.491
MOD_CK1_1	92	98	PF00069	0.341
MOD_CK2_1	325	331	PF00069	0.500
MOD_CK2_1	365	371	PF00069	0.496
MOD_CK2_1	559	565	PF00069	0.718
MOD_CK2_1	590	596	PF00069	0.447
MOD_CK2_1	685	691	PF00069	0.589
MOD_Cter_Amidation	143	146	PF01082	0.421
MOD_GlcNHglycan	1	4	PF01048	0.722
MOD_GlcNHglycan	163	166	PF01048	0.498
MOD_GlcNHglycan	192	195	PF01048	0.578
MOD_GlcNHglycan	244	247	PF01048	0.510
MOD_GlcNHglycan	301	304	PF01048	0.432
MOD_GlcNHglycan	367	370	PF01048	0.372
MOD_GlcNHglycan	507	510	PF01048	0.497
MOD_GlcNHglycan	607	610	PF01048	0.541
MOD_GlcNHglycan	667	671	PF01048	0.610
MOD_GlcNHglycan	673	676	PF01048	0.535
MOD_GSK3_1	157	164	PF00069	0.524
MOD_GSK3_1	177	184	PF00069	0.633
MOD_GSK3_1	190	197	PF00069	0.691
MOD_GSK3_1	333	340	PF00069	0.624
MOD_GSK3_1	627	634	PF00069	0.475
MOD_GSK3_1	713	720	PF00069	0.523
MOD_GSK3_1	746	753	PF00069	0.494
MOD_GSK3_1	88	95	PF00069	0.412
MOD_NEK2_1	225	230	PF00069	0.474
MOD_NEK2_1	241	246	PF00069	0.448
MOD_NEK2_1	250	255	PF00069	0.449
MOD_NEK2_1	307	312	PF00069	0.347
MOD_NEK2_1	425	430	PF00069	0.436
MOD_NEK2_1	457	462	PF00069	0.351
MOD_NEK2_1	48	53	PF00069	0.306
MOD_NEK2_1	514	519	PF00069	0.433
MOD_NEK2_1	588	593	PF00069	0.399
MOD_NEK2_1	671	676	PF00069	0.546
MOD_NEK2_1	87	92	PF00069	0.282
MOD_NEK2_1	97	102	PF00069	0.304
MOD_PIKK_1	264	270	PF00454	0.582
MOD_PIKK_1	682	688	PF00454	0.589
MOD_PKA_1	365	371	PF00069	0.393
MOD_PKA_2	242	248	PF00069	0.405
MOD_PKA_2	338	344	PF00069	0.482
MOD_PKA_2	365	371	PF00069	0.393
MOD_PKA_2	732	738	PF00069	0.359
MOD_PKB_1	337	345	PF00069	0.488
MOD_Plk_1	707	713	PF00069	0.666
MOD_Plk_4	205	211	PF00069	0.353
MOD_Plk_4	220	226	PF00069	0.374
MOD_Plk_4	307	313	PF00069	0.477
MOD_Plk_4	341	347	PF00069	0.560
MOD_Plk_4	355	361	PF00069	0.511
MOD_Plk_4	515	521	PF00069	0.481
MOD_Plk_4	57	63	PF00069	0.362
MOD_Plk_4	574	580	PF00069	0.435
MOD_Plk_4	590	596	PF00069	0.400
MOD_Plk_4	67	73	PF00069	0.298
MOD_Plk_4	97	103	PF00069	0.355
MOD_ProDKin_1	194	200	PF00069	0.681
MOD_ProDKin_1	202	208	PF00069	0.385
MOD_ProDKin_1	217	223	PF00069	0.381
MOD_ProDKin_1	418	424	PF00069	0.445
MOD_ProDKin_1	440	446	PF00069	0.400
MOD_ProDKin_1	92	98	PF00069	0.417
MOD_SUMO_rev_2	331	336	PF00179	0.510
TRG_DiLeu_BaEn_1	679	684	PF01217	0.433
TRG_DiLeu_BaEn_1	761	766	PF01217	0.318
TRG_DiLeu_BaLyEn_6	582	587	PF01217	0.488
TRG_ENDOCYTIC_2	173	176	PF00928	0.559
TRG_ENDOCYTIC_2	216	219	PF00928	0.478
TRG_ENDOCYTIC_2	314	317	PF00928	0.313
TRG_ENDOCYTIC_2	353	356	PF00928	0.349
TRG_ENDOCYTIC_2	521	524	PF00928	0.345
TRG_ER_diArg_1	145	147	PF00400	0.440
TRG_ER_diArg_1	322	324	PF00400	0.439
TRG_ER_diArg_1	364	366	PF00400	0.423
TRG_ER_diArg_1	618	621	PF00400	0.487
TRG_ER_diArg_1	767	770	PF00400	0.310
TRG_Pf-PMV_PEXEL_1	522	526	PF00026	0.411
TRG_Pf-PMV_PEXEL_1	721	726	PF00026	0.349

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5J2	Leptomonas seymouri	50%	100%
A0A1X0NR91	Trypanosomatidae	24%	100%
A0A3R7MGL1	Trypanosoma rangeli	27%	100%
A0A3S7X724	Leishmania donovani	83%	99%
A4HLX6	Leishmania braziliensis	73%	99%
A4I9A5	Leishmania infantum	83%	99%
C9ZJC1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	24%	100%
Q4Q3S1	Leishmania major	84%	100%
V5AV29	Trypanosoma cruzi	29%	100%