Diphthamide biosynthesis protein 2

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

diphthamide biosynthesis enzyme Dph1/Dph2 domain containing protein, putative

Species:

Leishmania mexicana

UniProt:

E9B480_LEIMU

TriTrypDb:

LmxM.32.2410

Length:

667

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B480

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B480

Function

Biological processes
Term	Name	Level	Count
GO:0006417	regulation of translation	6	11
GO:0006448	regulation of translational elongation	7	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009889	regulation of biosynthetic process	4	11
GO:0009987	cellular process	1	11
GO:0010468	regulation of gene expression	5	11
GO:0010556	regulation of macromolecule biosynthetic process	5	11
GO:0010608	post-transcriptional regulation of gene expression	6	11
GO:0017182	peptidyl-diphthamide metabolic process	7	11
GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine	4	11
GO:0018193	peptidyl-amino acid modification	5	11
GO:0018202	peptidyl-histidine modification	6	11
GO:0019222	regulation of metabolic process	3	11
GO:0019538	protein metabolic process	3	11
GO:0031323	regulation of cellular metabolic process	4	11
GO:0031326	regulation of cellular biosynthetic process	5	11
GO:0034248	regulation of amide metabolic process	5	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0050789	regulation of biological process	2	11
GO:0050794	regulation of cellular process	3	11
GO:0051171	regulation of nitrogen compound metabolic process	4	11
GO:0051246	regulation of protein metabolic process	5	11
GO:0060255	regulation of macromolecule metabolic process	4	11
GO:0065007	biological regulation	1	11
GO:0071704	organic substance metabolic process	2	11
GO:0080090	regulation of primary metabolic process	4	11
GO:1900247	regulation of cytoplasmic translational elongation	8	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0016740	transferase activity	2	11
GO:0016765	transferase activity, transferring alkyl or aryl (other than methyl) groups	3	11
GO:0090560	2-(3-amino-3-carboxypropyl)histidine synthase activity	4	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	264	268	PF00656	0.452
CLV_C14_Caspase3-7	387	391	PF00656	0.459
CLV_C14_Caspase3-7	508	512	PF00656	0.501
CLV_C14_Caspase3-7	602	606	PF00656	0.528
CLV_MEL_PAP_1	628	634	PF00089	0.617
CLV_NRD_NRD_1	174	176	PF00675	0.407
CLV_NRD_NRD_1	182	184	PF00675	0.355
CLV_NRD_NRD_1	226	228	PF00675	0.483
CLV_NRD_NRD_1	422	424	PF00675	0.329
CLV_NRD_NRD_1	563	565	PF00675	0.382
CLV_NRD_NRD_1	62	64	PF00675	0.451
CLV_PCSK_KEX2_1	174	176	PF00082	0.365
CLV_PCSK_KEX2_1	182	184	PF00082	0.328
CLV_PCSK_KEX2_1	226	228	PF00082	0.526
CLV_PCSK_KEX2_1	39	41	PF00082	0.565
CLV_PCSK_KEX2_1	422	424	PF00082	0.235
CLV_PCSK_KEX2_1	468	470	PF00082	0.235
CLV_PCSK_KEX2_1	509	511	PF00082	0.329
CLV_PCSK_KEX2_1	557	559	PF00082	0.494
CLV_PCSK_KEX2_1	563	565	PF00082	0.439
CLV_PCSK_KEX2_1	62	64	PF00082	0.451
CLV_PCSK_PC1ET2_1	39	41	PF00082	0.416
CLV_PCSK_PC1ET2_1	468	470	PF00082	0.235
CLV_PCSK_PC1ET2_1	509	511	PF00082	0.329
CLV_PCSK_PC1ET2_1	557	559	PF00082	0.535
CLV_PCSK_PC7_1	464	470	PF00082	0.235
CLV_PCSK_SKI1_1	2	6	PF00082	0.364
DEG_APCC_DBOX_1	205	213	PF00400	0.288
DEG_APCC_DBOX_1	364	372	PF00400	0.354
DEG_Nend_UBRbox_1	1	4	PF02207	0.296
DEG_SPOP_SBC_1	526	530	PF00917	0.530
DEG_SPOP_SBC_1	544	548	PF00917	0.269
DOC_MAPK_gen_1	468	475	PF00069	0.435
DOC_MAPK_gen_1	654	663	PF00069	0.442
DOC_MAPK_MEF2A_6	435	444	PF00069	0.435
DOC_MAPK_RevD_3	551	564	PF00069	0.317
DOC_PP4_FxxP_1	513	516	PF00568	0.448
DOC_USP7_MATH_1	125	129	PF00917	0.448
DOC_USP7_MATH_1	260	264	PF00917	0.657
DOC_USP7_MATH_1	295	299	PF00917	0.489
DOC_USP7_MATH_1	309	313	PF00917	0.417
DOC_USP7_MATH_1	343	347	PF00917	0.481
DOC_USP7_MATH_1	380	384	PF00917	0.500
DOC_USP7_MATH_1	526	530	PF00917	0.479
DOC_USP7_MATH_1	559	563	PF00917	0.416
DOC_WW_Pin1_4	291	296	PF00397	0.448
DOC_WW_Pin1_4	378	383	PF00397	0.472
DOC_WW_Pin1_4	516	521	PF00397	0.435
DOC_WW_Pin1_4	632	637	PF00397	0.487
LIG_14-3-3_CanoR_1	174	184	PF00244	0.406
LIG_14-3-3_CanoR_1	226	236	PF00244	0.484
LIG_14-3-3_CanoR_1	287	297	PF00244	0.597
LIG_14-3-3_CanoR_1	435	444	PF00244	0.423
LIG_14-3-3_CanoR_1	597	601	PF00244	0.532
LIG_deltaCOP1_diTrp_1	275	285	PF00928	0.425
LIG_FHA_1	147	153	PF00498	0.595
LIG_FHA_1	165	171	PF00498	0.468
LIG_FHA_1	215	221	PF00498	0.407
LIG_FHA_1	387	393	PF00498	0.475
LIG_FHA_1	454	460	PF00498	0.436
LIG_FHA_2	228	234	PF00498	0.605
LIG_FHA_2	270	276	PF00498	0.391
LIG_FHA_2	526	532	PF00498	0.520
LIG_FHA_2	572	578	PF00498	0.545
LIG_LIR_Apic_2	511	516	PF02991	0.449
LIG_LIR_Gen_1	300	310	PF02991	0.368
LIG_LIR_Gen_1	322	332	PF02991	0.420
LIG_LIR_Gen_1	491	500	PF02991	0.435
LIG_LIR_Gen_1	546	556	PF02991	0.354
LIG_LIR_Nem_3	300	306	PF02991	0.435
LIG_LIR_Nem_3	322	327	PF02991	0.526
LIG_LIR_Nem_3	450	455	PF02991	0.500
LIG_LIR_Nem_3	470	474	PF02991	0.350
LIG_LIR_Nem_3	491	496	PF02991	0.435
LIG_LIR_Nem_3	536	542	PF02991	0.431
LIG_LIR_Nem_3	546	552	PF02991	0.346
LIG_LIR_Nem_3	622	628	PF02991	0.491
LIG_NRBOX	458	464	PF00104	0.448
LIG_PCNA_PIPBox_1	614	623	PF02747	0.567
LIG_PCNA_yPIPBox_3	614	623	PF02747	0.508
LIG_SH2_CRK	539	543	PF00017	0.271
LIG_SH2_PTP2	137	140	PF00017	0.530
LIG_SH2_PTP2	625	628	PF00017	0.573
LIG_SH2_SRC	32	35	PF00017	0.447
LIG_SH2_STAP1	545	549	PF00017	0.365
LIG_SH2_STAP1	621	625	PF00017	0.529
LIG_SH2_STAT3	500	503	PF00017	0.482
LIG_SH2_STAT5	114	117	PF00017	0.514
LIG_SH2_STAT5	137	140	PF00017	0.470
LIG_SH2_STAT5	303	306	PF00017	0.335
LIG_SH2_STAT5	32	35	PF00017	0.447
LIG_SH2_STAT5	336	339	PF00017	0.349
LIG_SH2_STAT5	474	477	PF00017	0.442
LIG_SH2_STAT5	545	548	PF00017	0.459
LIG_SH2_STAT5	625	628	PF00017	0.573
LIG_SH3_3	196	202	PF00018	0.458
LIG_SH3_3	280	286	PF00018	0.460
LIG_SH3_3	493	499	PF00018	0.530
LIG_SH3_3	54	60	PF00018	0.604
LIG_SH3_3	81	87	PF00018	0.664
LIG_Sin3_3	166	173	PF02671	0.260
LIG_SUMO_SIM_anti_2	14	20	PF11976	0.373
LIG_SUMO_SIM_par_1	165	171	PF11976	0.479
LIG_SUMO_SIM_par_1	522	533	PF11976	0.465
LIG_SUMO_SIM_par_1	540	548	PF11976	0.382
MOD_CK1_1	128	134	PF00069	0.271
MOD_CK1_1	290	296	PF00069	0.433
MOD_CK1_1	562	568	PF00069	0.510
MOD_CK1_1	599	605	PF00069	0.686
MOD_CK2_1	269	275	PF00069	0.522
MOD_CK2_1	516	522	PF00069	0.269
MOD_CK2_1	562	568	PF00069	0.611
MOD_Cter_Amidation	466	469	PF01082	0.269
MOD_GlcNHglycan	177	180	PF01048	0.520
MOD_GlcNHglycan	272	275	PF01048	0.596
MOD_GlcNHglycan	312	315	PF01048	0.413
MOD_GlcNHglycan	382	385	PF01048	0.514
MOD_GlcNHglycan	396	399	PF01048	0.404
MOD_GlcNHglycan	437	440	PF01048	0.287
MOD_GlcNHglycan	591	594	PF01048	0.545
MOD_GlcNHglycan	601	604	PF01048	0.533
MOD_GlcNHglycan	663	666	PF01048	0.650
MOD_GlcNHglycan	88	91	PF01048	0.463
MOD_GSK3_1	21	28	PF00069	0.426
MOD_GSK3_1	227	234	PF00069	0.489
MOD_GSK3_1	287	294	PF00069	0.575
MOD_GSK3_1	354	361	PF00069	0.364
MOD_GSK3_1	378	385	PF00069	0.526
MOD_GSK3_1	430	437	PF00069	0.385
MOD_GSK3_1	46	53	PF00069	0.658
MOD_GSK3_1	526	533	PF00069	0.362
MOD_GSK3_1	562	569	PF00069	0.517
MOD_GSK3_1	571	578	PF00069	0.603
MOD_N-GLC_1	369	374	PF02516	0.447
MOD_NEK2_1	127	132	PF00069	0.273
MOD_NEK2_1	192	197	PF00069	0.443
MOD_NEK2_1	288	293	PF00069	0.598
MOD_NEK2_1	354	359	PF00069	0.409
MOD_NEK2_1	443	448	PF00069	0.371
MOD_PIKK_1	453	459	PF00454	0.402
MOD_PIKK_1	46	52	PF00454	0.653
MOD_PIKK_1	530	536	PF00454	0.479
MOD_PIKK_1	575	581	PF00454	0.669
MOD_PIKK_1	610	616	PF00454	0.553
MOD_PKA_1	182	188	PF00069	0.255
MOD_PKA_1	226	232	PF00069	0.400
MOD_PKA_2	128	134	PF00069	0.276
MOD_PKA_2	182	188	PF00069	0.413
MOD_PKA_2	226	232	PF00069	0.496
MOD_PKA_2	434	440	PF00069	0.287
MOD_PKA_2	562	568	PF00069	0.552
MOD_PKA_2	596	602	PF00069	0.526
MOD_Plk_1	164	170	PF00069	0.453
MOD_Plk_1	231	237	PF00069	0.447
MOD_Plk_1	244	250	PF00069	0.351
MOD_Plk_1	260	266	PF00069	0.533
MOD_Plk_1	354	360	PF00069	0.400
MOD_Plk_1	97	103	PF00069	0.269
MOD_Plk_2-3	261	267	PF00069	0.450
MOD_Plk_4	164	170	PF00069	0.298
MOD_Plk_4	21	27	PF00069	0.264
MOD_ProDKin_1	291	297	PF00069	0.441
MOD_ProDKin_1	378	384	PF00069	0.461
MOD_ProDKin_1	516	522	PF00069	0.269
MOD_ProDKin_1	632	638	PF00069	0.490
MOD_SUMO_for_1	45	48	PF00179	0.501
MOD_SUMO_rev_2	34	41	PF00179	0.600
TRG_DiLeu_BaEn_1	275	280	PF01217	0.273
TRG_DiLeu_BaEn_1	300	305	PF01217	0.310
TRG_DiLeu_BaLyEn_6	284	289	PF01217	0.259
TRG_DiLeu_BaLyEn_6	551	556	PF01217	0.330
TRG_ENDOCYTIC_2	303	306	PF00928	0.370
TRG_ENDOCYTIC_2	324	327	PF00928	0.370
TRG_ENDOCYTIC_2	329	332	PF00928	0.378
TRG_ENDOCYTIC_2	452	455	PF00928	0.339
TRG_ENDOCYTIC_2	539	542	PF00928	0.283
TRG_ENDOCYTIC_2	625	628	PF00928	0.573
TRG_ER_diArg_1	174	176	PF00400	0.383
TRG_ER_diArg_1	182	184	PF00400	0.373
TRG_ER_diArg_1	205	208	PF00400	0.305
TRG_ER_diArg_1	226	228	PF00400	0.526
TRG_ER_diArg_1	422	425	PF00400	0.293
TRG_Pf-PMV_PEXEL_1	373	377	PF00026	0.290

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8D8	Leptomonas seymouri	57%	86%
A0A1X0P3T2	Trypanosomatidae	40%	100%
A0A3S5ISG6	Trypanosoma rangeli	41%	100%
A0A3S7X6Z1	Leishmania donovani	89%	100%
A4HLV3	Leishmania braziliensis	72%	100%
D0A670	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
E9AHQ9	Leishmania infantum	88%	87%
Q4Q3U6	Leishmania major	88%	100%
V5BP75	Trypanosoma cruzi	40%	100%