Putative peptidase M20/M25/M40

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cytosolic nonspecific dipeptidase, putative

Species:

Leishmania mexicana

UniProt:

E9B3Z8_LEIMU

TriTrypDb:

LmxM.32.1610

Length:

472

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 6
Silverman et al.	no	yes: 3
Pissara et al.	yes	yes: 33

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 5

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 23
NetGPI	no	yes: 0, no: 23

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B3Z8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B3Z8

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	3
GO:0006807	nitrogen compound metabolic process	2	3
GO:0008152	metabolic process	1	3
GO:0019538	protein metabolic process	3	3
GO:0043170	macromolecule metabolic process	3	3
GO:0044238	primary metabolic process	2	3
GO:0071704	organic substance metabolic process	2	3
GO:1901564	organonitrogen compound metabolic process	3	3

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	24
GO:0016787	hydrolase activity	2	24
GO:0005488	binding	1	1
GO:0005506	iron ion binding	6	1
GO:0016491	oxidoreductase activity	2	1
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	1
GO:0043167	ion binding	2	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1
GO:0046914	transition metal ion binding	5	1
GO:0008233	peptidase activity	3	3
GO:0140096	catalytic activity, acting on a protein	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	295	299	PF00656	0.203
CLV_PCSK_SKI1_1	118	122	PF00082	0.243
CLV_PCSK_SKI1_1	210	214	PF00082	0.403
CLV_PCSK_SKI1_1	256	260	PF00082	0.379
CLV_PCSK_SKI1_1	450	454	PF00082	0.365
CLV_PCSK_SKI1_1	49	53	PF00082	0.442
DOC_CYCLIN_RxL_1	253	261	PF00134	0.363
DOC_CYCLIN_RxL_1	448	460	PF00134	0.403
DOC_MAPK_gen_1	181	189	PF00069	0.426
DOC_MAPK_gen_1	49	56	PF00069	0.436
DOC_MAPK_HePTP_8	302	314	PF00069	0.325
DOC_MAPK_MEF2A_6	183	191	PF00069	0.379
DOC_MAPK_MEF2A_6	305	314	PF00069	0.325
DOC_MAPK_MEF2A_6	330	339	PF00069	0.360
DOC_MAPK_MEF2A_6	77	85	PF00069	0.439
DOC_PP1_RVXF_1	254	260	PF00149	0.444
DOC_PP1_RVXF_1	365	371	PF00149	0.389
DOC_PP1_RVXF_1	451	458	PF00149	0.345
DOC_PP2B_LxvP_1	339	342	PF13499	0.300
DOC_PP4_FxxP_1	307	310	PF00568	0.426
DOC_USP7_MATH_1	141	145	PF00917	0.466
DOC_WW_Pin1_4	32	37	PF00397	0.439
DOC_WW_Pin1_4	378	383	PF00397	0.316
DOC_WW_Pin1_4	467	472	PF00397	0.453
DOC_WW_Pin1_4	75	80	PF00397	0.429
LIG_14-3-3_CanoR_1	330	336	PF00244	0.375
LIG_Actin_WH2_2	321	338	PF00022	0.403
LIG_AP2alpha_1	282	286	PF02296	0.203
LIG_APCC_ABBA_1	279	284	PF00400	0.246
LIG_BIR_II_1	1	5	PF00653	0.525
LIG_BRCT_BRCA1_1	167	171	PF00533	0.369
LIG_BRCT_BRCA1_1	34	38	PF00533	0.529
LIG_BRCT_BRCA1_1	453	457	PF00533	0.203
LIG_BRCT_BRCA1_1	67	71	PF00533	0.340
LIG_FHA_1	204	210	PF00498	0.368
LIG_FHA_1	211	217	PF00498	0.368
LIG_FHA_1	236	242	PF00498	0.409
LIG_FHA_1	252	258	PF00498	0.281
LIG_FHA_1	379	385	PF00498	0.298
LIG_FHA_1	42	48	PF00498	0.498
LIG_FHA_1	429	435	PF00498	0.361
LIG_FHA_1	454	460	PF00498	0.362
LIG_FHA_1	51	57	PF00498	0.440
LIG_FHA_1	76	82	PF00498	0.419
LIG_FHA_2	219	225	PF00498	0.379
LIG_LIR_Gen_1	133	142	PF02991	0.408
LIG_LIR_Gen_1	26	36	PF02991	0.413
LIG_LIR_Gen_1	261	271	PF02991	0.437
LIG_LIR_Gen_1	3	13	PF02991	0.433
LIG_LIR_Gen_1	445	452	PF02991	0.376
LIG_LIR_Gen_1	68	79	PF02991	0.466
LIG_LIR_Nem_3	133	139	PF02991	0.378
LIG_LIR_Nem_3	26	31	PF02991	0.410
LIG_LIR_Nem_3	261	266	PF02991	0.359
LIG_LIR_Nem_3	3	9	PF02991	0.435
LIG_LIR_Nem_3	445	449	PF02991	0.376
LIG_LIR_Nem_3	68	74	PF02991	0.390
LIG_LIR_Nem_3	78	83	PF02991	0.367
LIG_LRP6_Inhibitor_1	324	330	PF00058	0.446
LIG_MYND_1	105	109	PF01753	0.368
LIG_MYND_1	382	386	PF01753	0.376
LIG_PCNA_yPIPBox_3	421	434	PF02747	0.307
LIG_Pex14_2	282	286	PF04695	0.393
LIG_Pex14_2	38	42	PF04695	0.552
LIG_PTB_Apo_2	358	365	PF02174	0.343
LIG_PTB_Phospho_1	358	364	PF10480	0.343
LIG_SH2_CRK	400	404	PF00017	0.312
LIG_SH2_PTP2	28	31	PF00017	0.427
LIG_SH2_SRC	28	31	PF00017	0.441
LIG_SH2_STAP1	200	204	PF00017	0.368
LIG_SH2_STAP1	451	455	PF00017	0.203
LIG_SH2_STAT5	263	266	PF00017	0.437
LIG_SH2_STAT5	269	272	PF00017	0.437
LIG_SH2_STAT5	28	31	PF00017	0.427
LIG_SH3_1	103	109	PF00018	0.368
LIG_SH3_3	103	109	PF00018	0.368
LIG_SH3_3	254	260	PF00018	0.408
LIG_SH3_3	356	362	PF00018	0.293
LIG_SH3_3	369	375	PF00018	0.251
LIG_SH3_3	430	436	PF00018	0.298
LIG_SUMO_SIM_anti_2	143	149	PF11976	0.312
LIG_SUMO_SIM_par_1	157	163	PF11976	0.366
LIG_TRAF2_1	164	167	PF00917	0.360
LIG_TRAF2_2	32	37	PF00917	0.384
LIG_TYR_ITIM	198	203	PF00017	0.312
LIG_UBA3_1	388	397	PF00899	0.203
LIG_UBA3_1	55	60	PF00899	0.332
MOD_CDK_SPK_2	467	472	PF00069	0.457
MOD_CDK_SPxxK_3	378	385	PF00069	0.325
MOD_CK1_1	140	146	PF00069	0.309
MOD_CK1_1	225	231	PF00069	0.403
MOD_CK1_1	273	279	PF00069	0.418
MOD_CK1_1	293	299	PF00069	0.231
MOD_CK2_1	218	224	PF00069	0.379
MOD_GlcNHglycan	139	142	PF01048	0.316
MOD_GlcNHglycan	166	170	PF01048	0.379
MOD_GlcNHglycan	194	197	PF01048	0.371
MOD_GlcNHglycan	227	230	PF01048	0.367
MOD_GlcNHglycan	25	28	PF01048	0.459
MOD_GlcNHglycan	295	298	PF01048	0.445
MOD_GSK3_1	137	144	PF00069	0.498
MOD_GSK3_1	218	225	PF00069	0.493
MOD_GSK3_1	308	315	PF00069	0.496
MOD_GSK3_1	331	338	PF00069	0.375
MOD_GSK3_1	453	460	PF00069	0.381
MOD_N-GLC_1	198	203	PF02516	0.487
MOD_N-GLC_1	32	37	PF02516	0.451
MOD_N-GLC_2	377	379	PF02516	0.442
MOD_NEK2_1	198	203	PF00069	0.401
MOD_NEK2_1	205	210	PF00069	0.343
MOD_NEK2_1	222	227	PF00069	0.370
MOD_NEK2_1	312	317	PF00069	0.430
MOD_NEK2_1	335	340	PF00069	0.309
MOD_NEK2_1	428	433	PF00069	0.400
MOD_NEK2_1	457	462	PF00069	0.433
MOD_PIKK_1	198	204	PF00454	0.487
MOD_PIKK_1	348	354	PF00454	0.455
MOD_Plk_1	17	23	PF00069	0.427
MOD_Plk_1	198	204	PF00069	0.485
MOD_Plk_2-3	167	173	PF00069	0.325
MOD_Plk_4	143	149	PF00069	0.442
MOD_Plk_4	17	23	PF00069	0.414
MOD_Plk_4	453	459	PF00069	0.310
MOD_Plk_4	91	97	PF00069	0.438
MOD_ProDKin_1	32	38	PF00069	0.444
MOD_ProDKin_1	378	384	PF00069	0.316
MOD_ProDKin_1	75	81	PF00069	0.425
MOD_SUMO_rev_2	250	254	PF00179	0.402
TRG_ENDOCYTIC_2	136	139	PF00928	0.441
TRG_ENDOCYTIC_2	200	203	PF00928	0.375
TRG_ENDOCYTIC_2	263	266	PF00928	0.417
TRG_ENDOCYTIC_2	28	31	PF00928	0.427
TRG_ENDOCYTIC_2	69	72	PF00928	0.433
TRG_ENDOCYTIC_2	98	101	PF00928	0.368
TRG_NES_CRM1_1	182	194	PF08389	0.497

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1Q9	Leptomonas seymouri	83%	100%
A0A0N1PBD8	Leptomonas seymouri	74%	100%
A0A0S4JJE7	Bodo saltans	58%	100%
A0A1X0P1Q0	Trypanosomatidae	67%	100%
A0A381MQ29	Leishmania infantum	74%	100%
A0A3Q8IFP2	Leishmania donovani	74%	83%
A0A3R7KSZ4	Trypanosoma rangeli	63%	100%
A0A3S7X4P6	Leishmania donovani	74%	100%
A0A3S7X6U2	Leishmania donovani	95%	100%
A4HJG9	Leishmania braziliensis	74%	100%
A4HJH1	Leishmania braziliensis	74%	100%
A4HJI4	Leishmania braziliensis	73%	100%
A4HLM2	Leishmania braziliensis	86%	100%
A4I933	Leishmania infantum	95%	100%
B2KZE7	Corynebacterium striatum	29%	100%
C9ZQ48	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	99%
D0A536	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	99%
E9B1Y8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	77%	100%
E9B201	Leishmania mexicana (strain MHOM/GT/2001/U1103)	74%	100%
P43616	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	98%
Q3ZC84	Bos taurus	26%	99%
Q4Q426	Leishmania major	94%	100%
Q4Q660	Leishmania major	74%	100%
Q4Q673	Leishmania major	74%	100%
Q5R432	Pongo abelii	26%	99%
Q6Q0N1	Rattus norvegicus	27%	99%
Q96KP4	Homo sapiens	27%	99%
Q9D1A2	Mus musculus	27%	99%
V5BHA2	Trypanosoma cruzi	63%	99%