Nfu_N domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Scaffold protein Nfu/NifU N terminal/NifU-like domain containing protein, putative

Species:

Leishmania mexicana

UniProt:

E9B3N3_LEIMU

TriTrypDb:

LmxM.32.0540

Length:

426

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	2
GO:0016020	membrane	2	1

Expansion

Sequence features

E9B3N3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B3N3

Function

Biological processes
Term	Name	Level	Count
GO:0006790	sulfur compound metabolic process	3	10
GO:0008152	metabolic process	1	10
GO:0009987	cellular process	1	10
GO:0016043	cellular component organization	3	10
GO:0016226	iron-sulfur cluster assembly	4	10
GO:0022607	cellular component assembly	4	10
GO:0031163	metallo-sulfur cluster assembly	5	10
GO:0044237	cellular metabolic process	2	10
GO:0071840	cellular component organization or biogenesis	2	10
GO:0006807	nitrogen compound metabolic process	2	1
GO:0019538	protein metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044238	primary metabolic process	2	1
GO:0051604	protein maturation	4	1
GO:0071704	organic substance metabolic process	2	1
GO:0097428	protein maturation by iron-sulfur cluster transfer	5	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	10
GO:0005506	iron ion binding	6	10
GO:0043167	ion binding	2	10
GO:0043169	cation binding	3	10
GO:0046872	metal ion binding	4	10
GO:0046914	transition metal ion binding	5	10
GO:0051536	iron-sulfur cluster binding	3	10
GO:0051540	metal cluster binding	2	10
GO:0051539	4 iron, 4 sulfur cluster binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	116	120	PF00656	0.264
CLV_C14_Caspase3-7	255	259	PF00656	0.511
CLV_NRD_NRD_1	22	24	PF00675	0.627
CLV_NRD_NRD_1	405	407	PF00675	0.681
CLV_PCSK_KEX2_1	113	115	PF00082	0.279
CLV_PCSK_KEX2_1	22	24	PF00082	0.627
CLV_PCSK_PC1ET2_1	113	115	PF00082	0.347
CLV_PCSK_SKI1_1	330	334	PF00082	0.264
CLV_PCSK_SKI1_1	45	49	PF00082	0.476
DEG_Nend_UBRbox_1	1	4	PF02207	0.470
DOC_CKS1_1	52	57	PF01111	0.338
DOC_MAPK_gen_1	308	317	PF00069	0.527
DOC_MAPK_MEF2A_6	2	10	PF00069	0.579
DOC_MAPK_RevD_3	98	114	PF00069	0.279
DOC_PP1_RVXF_1	283	290	PF00149	0.493
DOC_PP4_FxxP_1	69	72	PF00568	0.395
DOC_USP7_MATH_1	146	150	PF00917	0.479
DOC_USP7_MATH_1	209	213	PF00917	0.535
DOC_USP7_MATH_1	243	247	PF00917	0.560
DOC_USP7_MATH_1	347	351	PF00917	0.565
DOC_USP7_MATH_2	254	260	PF00917	0.491
DOC_USP7_UBL2_3	403	407	PF12436	0.560
DOC_WW_Pin1_4	14	19	PF00397	0.538
DOC_WW_Pin1_4	180	185	PF00397	0.746
DOC_WW_Pin1_4	194	199	PF00397	0.543
DOC_WW_Pin1_4	360	365	PF00397	0.587
DOC_WW_Pin1_4	384	389	PF00397	0.650
DOC_WW_Pin1_4	51	56	PF00397	0.340
DOC_WW_Pin1_4	84	89	PF00397	0.279
LIG_14-3-3_CanoR_1	305	315	PF00244	0.445
LIG_14-3-3_CanoR_1	337	345	PF00244	0.576
LIG_FHA_1	105	111	PF00498	0.312
LIG_FHA_1	229	235	PF00498	0.390
LIG_FHA_1	310	316	PF00498	0.540
LIG_FHA_1	364	370	PF00498	0.714
LIG_FHA_1	44	50	PF00498	0.439
LIG_FHA_2	138	144	PF00498	0.547
LIG_FHA_2	250	256	PF00498	0.622
LIG_FHA_2	311	317	PF00498	0.429
LIG_FHA_2	52	58	PF00498	0.279
LIG_FHA_2	90	96	PF00498	0.374
LIG_GBD_Chelix_1	89	97	PF00786	0.279
LIG_LIR_Apic_2	66	72	PF02991	0.374
LIG_LIR_Apic_2	82	88	PF02991	0.303
LIG_LIR_Gen_1	107	115	PF02991	0.341
LIG_LIR_Gen_1	216	226	PF02991	0.391
LIG_LIR_Gen_1	339	347	PF02991	0.521
LIG_LIR_Gen_1	46	55	PF02991	0.310
LIG_LIR_Nem_3	107	112	PF02991	0.341
LIG_LIR_Nem_3	216	222	PF02991	0.372
LIG_LIR_Nem_3	283	289	PF02991	0.444
LIG_LIR_Nem_3	339	345	PF02991	0.519
LIG_LIR_Nem_3	399	405	PF02991	0.653
LIG_LIR_Nem_3	46	51	PF02991	0.418
LIG_LIR_Nem_3	57	63	PF02991	0.328
LIG_MYND_1	180	184	PF01753	0.572
LIG_Pex14_2	127	131	PF04695	0.464
LIG_SH2_CRK	402	406	PF00017	0.642
LIG_SH2_STAT5	126	129	PF00017	0.509
LIG_SH2_STAT5	63	66	PF00017	0.381
LIG_SH2_STAT5	85	88	PF00017	0.347
LIG_SH3_3	12	18	PF00018	0.671
LIG_SH3_CIN85_PxpxPR_1	166	171	PF14604	0.467
LIG_SUMO_SIM_anti_2	258	267	PF11976	0.408
LIG_SUMO_SIM_anti_2	312	319	PF11976	0.498
LIG_SUMO_SIM_anti_2	7	12	PF11976	0.467
LIG_SUMO_SIM_par_1	258	267	PF11976	0.408
LIG_SUMO_SIM_par_1	312	319	PF11976	0.527
LIG_TRAF2_1	140	143	PF00917	0.609
MOD_CK1_1	108	114	PF00069	0.410
MOD_CK1_1	17	23	PF00069	0.480
MOD_CK1_1	197	203	PF00069	0.670
MOD_CK1_1	309	315	PF00069	0.438
MOD_CK1_1	32	38	PF00069	0.690
MOD_CK1_1	336	342	PF00069	0.515
MOD_CK1_1	363	369	PF00069	0.643
MOD_CK1_1	389	395	PF00069	0.660
MOD_CK1_1	81	87	PF00069	0.354
MOD_CK2_1	137	143	PF00069	0.606
MOD_CK2_1	197	203	PF00069	0.580
MOD_CK2_1	249	255	PF00069	0.573
MOD_CK2_1	337	343	PF00069	0.566
MOD_CK2_1	409	415	PF00069	0.628
MOD_CK2_1	89	95	PF00069	0.374
MOD_GlcNHglycan	226	229	PF01048	0.351
MOD_GlcNHglycan	235	238	PF01048	0.455
MOD_GlcNHglycan	339	342	PF01048	0.448
MOD_GlcNHglycan	393	396	PF01048	0.638
MOD_GlcNHglycan	398	401	PF01048	0.576
MOD_GlcNHglycan	411	414	PF01048	0.655
MOD_GlcNHglycan	420	423	PF01048	0.665
MOD_GSK3_1	101	108	PF00069	0.422
MOD_GSK3_1	174	181	PF00069	0.527
MOD_GSK3_1	192	199	PF00069	0.573
MOD_GSK3_1	224	231	PF00069	0.481
MOD_GSK3_1	24	31	PF00069	0.632
MOD_GSK3_1	303	310	PF00069	0.451
MOD_GSK3_1	333	340	PF00069	0.553
MOD_GSK3_1	359	366	PF00069	0.619
MOD_GSK3_1	380	387	PF00069	0.664
MOD_NEK2_1	224	229	PF00069	0.395
MOD_NEK2_1	268	273	PF00069	0.467
MOD_NEK2_1	6	11	PF00069	0.704
MOD_NEK2_1	79	84	PF00069	0.340
MOD_NEK2_1	89	94	PF00069	0.372
MOD_NEK2_2	303	308	PF00069	0.486
MOD_NEK2_2	43	48	PF00069	0.443
MOD_PIKK_1	146	152	PF00454	0.567
MOD_PIKK_1	197	203	PF00454	0.668
MOD_PKA_2	319	325	PF00069	0.488
MOD_PKA_2	32	38	PF00069	0.666
MOD_PKA_2	336	342	PF00069	0.515
MOD_Plk_1	101	107	PF00069	0.279
MOD_Plk_1	185	191	PF00069	0.647
MOD_Plk_1	333	339	PF00069	0.384
MOD_Plk_4	105	111	PF00069	0.446
MOD_Plk_4	209	215	PF00069	0.513
MOD_Plk_4	310	316	PF00069	0.523
MOD_Plk_4	32	38	PF00069	0.638
MOD_Plk_4	6	12	PF00069	0.513
MOD_Plk_4	73	79	PF00069	0.395
MOD_Plk_4	81	87	PF00069	0.395
MOD_Plk_4	89	95	PF00069	0.395
MOD_ProDKin_1	14	20	PF00069	0.540
MOD_ProDKin_1	180	186	PF00069	0.746
MOD_ProDKin_1	194	200	PF00069	0.543
MOD_ProDKin_1	360	366	PF00069	0.588
MOD_ProDKin_1	384	390	PF00069	0.647
MOD_ProDKin_1	51	57	PF00069	0.340
MOD_ProDKin_1	84	90	PF00069	0.279
MOD_SUMO_rev_2	216	224	PF00179	0.371
TRG_DiLeu_BaEn_1	260	265	PF01217	0.280
TRG_ENDOCYTIC_2	402	405	PF00928	0.643
TRG_ER_diArg_1	21	23	PF00400	0.633

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6M8	Leptomonas seymouri	64%	90%
A0A0S4J2M4	Bodo saltans	40%	94%
A0A3S7X6H5	Leishmania donovani	90%	95%
A4HL91	Leishmania braziliensis	75%	100%
A4I8R7	Leishmania infantum	90%	95%
D0A4Q8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	100%
Q4Q4F4	Leishmania major	91%	100%
V5B2F6	Trypanosoma cruzi	49%	94%