DNA-directed primase/polymerase protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

PrimPol-like protein 1, putative

Species:

Leishmania mexicana

UniProt:

E9B3I0_LEIMU

TriTrypDb:

LmxM.32.0030

Length:

556

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005759	mitochondrial matrix	5	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B3I0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B3I0

Function

Biological processes
Term	Name	Level	Count
GO:0000731	DNA synthesis involved in DNA repair	6	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006281	DNA repair	5	11
GO:0006301	postreplication repair	6	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009059	macromolecule biosynthetic process	4	11
GO:0009987	cellular process	1	11
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0019985	translesion synthesis	7	11
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034654	nucleobase-containing compound biosynthetic process	4	11
GO:0042276	error-prone translesion synthesis	8	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0046483	heterocycle metabolic process	3	11
GO:0050896	response to stimulus	1	11
GO:0051716	cellular response to stimulus	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0071897	DNA biosynthetic process	5	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11
GO:0006260	DNA replication	5	1
GO:0006261	DNA-templated DNA replication	6	1
GO:0006264	mitochondrial DNA replication	6	1
GO:0009314	response to radiation	3	1
GO:0009411	response to UV	5	1
GO:0009416	response to light stimulus	4	1
GO:0009628	response to abiotic stimulus	2	1
GO:0031297	replication fork processing	6	1
GO:0032042	mitochondrial DNA metabolic process	5	1
GO:0045005	DNA-templated DNA replication maintenance of fidelity	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0003887	DNA-directed DNA polymerase activity	5	11
GO:0003896	DNA primase activity	7	11
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	11
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016779	nucleotidyltransferase activity	4	11
GO:0034061	DNA polymerase activity	4	11
GO:0034062	5'-3' RNA polymerase activity	5	11
GO:0097747	RNA polymerase activity	4	11
GO:0140097	catalytic activity, acting on DNA	3	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:0003682	chromatin binding	2	1
GO:0005488	binding	1	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	173	177	PF00656	0.625
CLV_C14_Caspase3-7	234	238	PF00656	0.299
CLV_C14_Caspase3-7	433	437	PF00656	0.673
CLV_NRD_NRD_1	189	191	PF00675	0.609
CLV_NRD_NRD_1	204	206	PF00675	0.349
CLV_NRD_NRD_1	352	354	PF00675	0.457
CLV_NRD_NRD_1	530	532	PF00675	0.356
CLV_NRD_NRD_1	75	77	PF00675	0.577
CLV_NRD_NRD_1	86	88	PF00675	0.656
CLV_PCSK_KEX2_1	189	191	PF00082	0.609
CLV_PCSK_KEX2_1	204	206	PF00082	0.349
CLV_PCSK_KEX2_1	352	354	PF00082	0.454
CLV_PCSK_KEX2_1	530	532	PF00082	0.257
CLV_PCSK_KEX2_1	75	77	PF00082	0.569
CLV_PCSK_KEX2_1	86	88	PF00082	0.590
CLV_PCSK_PC1ET2_1	86	88	PF00082	0.590
CLV_PCSK_SKI1_1	108	112	PF00082	0.346
CLV_PCSK_SKI1_1	119	123	PF00082	0.358
CLV_PCSK_SKI1_1	190	194	PF00082	0.614
CLV_PCSK_SKI1_1	418	422	PF00082	0.538
DEG_APCC_DBOX_1	118	126	PF00400	0.472
DEG_Nend_Nbox_1	1	3	PF02207	0.406
DOC_CYCLIN_RxL_1	493	503	PF00134	0.462
DOC_MAPK_DCC_7	418	428	PF00069	0.453
DOC_MAPK_gen_1	137	146	PF00069	0.379
DOC_MAPK_gen_1	374	382	PF00069	0.342
DOC_MAPK_gen_1	418	428	PF00069	0.453
DOC_MAPK_gen_1	515	524	PF00069	0.467
DOC_MAPK_HePTP_8	136	148	PF00069	0.290
DOC_MAPK_MEF2A_6	139	148	PF00069	0.372
DOC_MAPK_MEF2A_6	518	526	PF00069	0.492
DOC_PP1_RVXF_1	187	194	PF00149	0.455
DOC_PP1_RVXF_1	496	503	PF00149	0.433
DOC_PP4_FxxP_1	325	328	PF00568	0.380
DOC_USP7_MATH_1	235	239	PF00917	0.428
DOC_USP7_MATH_1	307	311	PF00917	0.390
DOC_USP7_MATH_1	403	407	PF00917	0.450
DOC_USP7_MATH_1	55	59	PF00917	0.377
DOC_WW_Pin1_4	41	46	PF00397	0.347
DOC_WW_Pin1_4	544	549	PF00397	0.578
DOC_WW_Pin1_4	76	81	PF00397	0.682
DOC_WW_Pin1_4	98	103	PF00397	0.511
LIG_14-3-3_CanoR_1	344	348	PF00244	0.463
LIG_14-3-3_CanoR_1	453	462	PF00244	0.510
LIG_14-3-3_CanoR_1	498	503	PF00244	0.497
LIG_14-3-3_CanoR_1	530	534	PF00244	0.534
LIG_BRCT_BRCA1_1	50	54	PF00533	0.345
LIG_Clathr_ClatBox_1	178	182	PF01394	0.455
LIG_FAT_LD_1	407	415	PF03623	0.214
LIG_FHA_1	186	192	PF00498	0.441
LIG_FHA_1	226	232	PF00498	0.305
LIG_FHA_1	293	299	PF00498	0.441
LIG_FHA_1	430	436	PF00498	0.583
LIG_FHA_1	459	465	PF00498	0.594
LIG_FHA_1	467	473	PF00498	0.464
LIG_FHA_2	5	11	PF00498	0.471
LIG_Integrin_RGD_1	34	36	PF01839	0.343
LIG_LIR_Apic_2	246	250	PF02991	0.418
LIG_LIR_Apic_2	544	548	PF02991	0.451
LIG_LIR_Gen_1	198	208	PF02991	0.472
LIG_LIR_Gen_1	259	267	PF02991	0.411
LIG_LIR_Gen_1	44	55	PF02991	0.298
LIG_LIR_Gen_1	92	102	PF02991	0.516
LIG_LIR_Nem_3	198	203	PF02991	0.453
LIG_LIR_Nem_3	259	264	PF02991	0.420
LIG_LIR_Nem_3	346	350	PF02991	0.303
LIG_LIR_Nem_3	44	50	PF02991	0.279
LIG_LIR_Nem_3	92	97	PF02991	0.521
LIG_LYPXL_SIV_4	478	486	PF13949	0.257
LIG_PCNA_yPIPBox_3	18	30	PF02747	0.338
LIG_Pex14_1	331	335	PF04695	0.396
LIG_Pex14_2	261	265	PF04695	0.386
LIG_Pex14_2	301	305	PF04695	0.409
LIG_Pex14_2	50	54	PF04695	0.345
LIG_Pex14_2	8	12	PF04695	0.340
LIG_REV1ctd_RIR_1	322	327	PF16727	0.361
LIG_SH2_CRK	479	483	PF00017	0.397
LIG_SH2_CRK	545	549	PF00017	0.531
LIG_SH2_NCK_1	479	483	PF00017	0.466
LIG_SH2_SRC	133	136	PF00017	0.376
LIG_SH2_SRC	145	148	PF00017	0.280
LIG_SH2_STAP1	479	483	PF00017	0.415
LIG_SH2_STAT5	145	148	PF00017	0.318
LIG_SH2_STAT5	283	286	PF00017	0.287
LIG_SH2_STAT5	349	352	PF00017	0.441
LIG_SH2_STAT5	387	390	PF00017	0.387
LIG_SH2_STAT5	509	512	PF00017	0.471
LIG_SH2_STAT5	523	526	PF00017	0.416
LIG_SH3_2	348	353	PF14604	0.314
LIG_SH3_3	345	351	PF00018	0.338
LIG_SH3_3	486	492	PF00018	0.475
LIG_SUMO_SIM_anti_2	406	413	PF11976	0.282
LIG_SUMO_SIM_par_1	159	171	PF11976	0.640
LIG_SUMO_SIM_par_1	460	465	PF11976	0.481
LIG_TRAF2_1	171	174	PF00917	0.580
LIG_TRAF2_1	7	10	PF00917	0.343
LIG_UBA3_1	366	374	PF00899	0.217
LIG_WRC_WIRS_1	236	241	PF05994	0.378
LIG_WRC_WIRS_1	499	504	PF05994	0.484
LIG_WW_3	547	551	PF00397	0.379
MOD_CDC14_SPxK_1	547	550	PF00782	0.372
MOD_CDK_SPxK_1	544	550	PF00069	0.404
MOD_CK1_1	120	126	PF00069	0.411
MOD_CK1_1	167	173	PF00069	0.606
MOD_CK1_1	294	300	PF00069	0.425
MOD_CK1_1	314	320	PF00069	0.236
MOD_CK1_1	401	407	PF00069	0.373
MOD_CK1_1	41	47	PF00069	0.584
MOD_CK1_1	451	457	PF00069	0.657
MOD_CK1_1	544	550	PF00069	0.475
MOD_CK1_1	58	64	PF00069	0.449
MOD_CK2_1	120	126	PF00069	0.552
MOD_CK2_1	167	173	PF00069	0.712
MOD_CK2_1	388	394	PF00069	0.502
MOD_CK2_1	4	10	PF00069	0.469
MOD_CK2_1	446	452	PF00069	0.755
MOD_CK2_1	53	59	PF00069	0.310
MOD_GlcNHglycan	233	236	PF01048	0.508
MOD_GlcNHglycan	398	401	PF01048	0.490
MOD_GlcNHglycan	455	458	PF01048	0.601
MOD_GlcNHglycan	55	58	PF01048	0.404
MOD_GSK3_1	120	127	PF00069	0.448
MOD_GSK3_1	164	171	PF00069	0.638
MOD_GSK3_1	231	238	PF00069	0.381
MOD_GSK3_1	307	314	PF00069	0.343
MOD_GSK3_1	451	458	PF00069	0.717
MOD_GSK3_1	466	473	PF00069	0.333
MOD_GSK3_1	500	507	PF00069	0.330
MOD_GSK3_1	525	532	PF00069	0.523
MOD_GSK3_1	58	65	PF00069	0.478
MOD_LATS_1	496	502	PF00433	0.414
MOD_N-GLC_1	152	157	PF02516	0.575
MOD_N-GLC_1	314	319	PF02516	0.266
MOD_N-GLC_1	466	471	PF02516	0.307
MOD_N-GLC_2	227	229	PF02516	0.381
MOD_NEK2_1	291	296	PF00069	0.440
MOD_NEK2_1	410	415	PF00069	0.293
MOD_NEK2_1	471	476	PF00069	0.391
MOD_NEK2_1	500	505	PF00069	0.300
MOD_NEK2_2	369	374	PF00069	0.327
MOD_PIKK_1	101	107	PF00454	0.483
MOD_PIKK_1	277	283	PF00454	0.323
MOD_PIKK_1	314	320	PF00454	0.541
MOD_PIKK_1	466	472	PF00454	0.426
MOD_PIKK_1	477	483	PF00454	0.453
MOD_PKA_2	311	317	PF00069	0.266
MOD_PKA_2	343	349	PF00069	0.472
MOD_PKA_2	504	510	PF00069	0.482
MOD_PKA_2	529	535	PF00069	0.578
MOD_PKB_1	241	249	PF00069	0.505
MOD_Plk_1	152	158	PF00069	0.405
MOD_Plk_1	217	223	PF00069	0.450
MOD_Plk_1	314	320	PF00069	0.322
MOD_Plk_1	451	457	PF00069	0.717
MOD_Plk_1	466	472	PF00069	0.287
MOD_Plk_1	58	64	PF00069	0.542
MOD_Plk_1	92	98	PF00069	0.633
MOD_Plk_4	174	180	PF00069	0.500
MOD_Plk_4	235	241	PF00069	0.336
MOD_Plk_4	243	249	PF00069	0.388
MOD_Plk_4	256	262	PF00069	0.456
MOD_Plk_4	376	382	PF00069	0.384
MOD_Plk_4	410	416	PF00069	0.368
MOD_Plk_4	424	430	PF00069	0.429
MOD_ProDKin_1	41	47	PF00069	0.329
MOD_ProDKin_1	544	550	PF00069	0.578
MOD_ProDKin_1	76	82	PF00069	0.682
MOD_ProDKin_1	98	104	PF00069	0.503
TRG_DiLeu_BaEn_1	174	179	PF01217	0.502
TRG_DiLeu_BaEn_1	59	64	PF01217	0.520
TRG_DiLeu_BaEn_1	92	97	PF01217	0.641
TRG_DiLeu_BaEn_2	528	534	PF01217	0.480
TRG_DiLeu_BaLyEn_6	547	552	PF01217	0.375
TRG_ENDOCYTIC_2	133	136	PF00928	0.306
TRG_ENDOCYTIC_2	145	148	PF00928	0.318
TRG_ENDOCYTIC_2	200	203	PF00928	0.461
TRG_ENDOCYTIC_2	332	335	PF00928	0.428
TRG_ENDOCYTIC_2	479	482	PF00928	0.399
TRG_ENDOCYTIC_2	523	526	PF00928	0.449
TRG_ER_diArg_1	189	191	PF00400	0.501
TRG_ER_diArg_1	203	205	PF00400	0.295
TRG_ER_diArg_1	351	353	PF00400	0.449
TRG_ER_diArg_1	87	90	PF00400	0.583
TRG_NLS_Bipartite_1	75	91	PF00514	0.674
TRG_NLS_MonoCore_2	85	90	PF00514	0.434
TRG_NLS_MonoExtC_3	86	91	PF00514	0.583
TRG_NLS_MonoExtN_4	86	91	PF00514	0.428

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCE0	Leptomonas seymouri	56%	98%
A0A0S4IKK2	Bodo saltans	28%	83%
A0A3R7MZJ4	Trypanosoma rangeli	37%	86%
A0A3S5H7T6	Leishmania donovani	88%	100%
A4HL39	Leishmania braziliensis	76%	100%
A4I8L9	Leishmania infantum	89%	100%
C9ZPU3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	91%
Q4Q4M0	Leishmania major	88%	100%
V5DB07	Trypanosoma cruzi	35%	91%