DNA helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

DNA helicase

Gene product:

TFIIH basal transcription factor complex helicase XPB subunit, putative

Species:

Leishmania mexicana

UniProt:

E9B3H0_LEIMU

TriTrypDb:

LmxM.31.3920

Length:

806

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000109	nucleotide-excision repair complex	3	1
GO:0000112	nucleotide-excision repair factor 3 complex	4	1
GO:0005667	transcription regulator complex	2	1
GO:0005675	transcription factor TFIIH holo complex	4	1
GO:0032806	carboxy-terminal domain protein kinase complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0032993	protein-DNA complex	2	1
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	1
GO:0090575	RNA polymerase II transcription regulator complex	3	1
GO:0097550	transcription preinitiation complex	3	1
GO:0140513	nuclear protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1902554	serine/threonine protein kinase complex	6	1
GO:1902911	protein kinase complex	5	1
GO:1990234	transferase complex	3	1

Expansion

Sequence features

E9B3H0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B3H0

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	5
GO:0006352	DNA-templated transcription initiation	6	5
GO:0006367	transcription initiation at RNA polymerase II promoter	7	5
GO:0006725	cellular aromatic compound metabolic process	3	5
GO:0006807	nitrogen compound metabolic process	2	5
GO:0008152	metabolic process	1	5
GO:0009058	biosynthetic process	2	5
GO:0009059	macromolecule biosynthetic process	4	5
GO:0009987	cellular process	1	5
GO:0016070	RNA metabolic process	5	5
GO:0018130	heterocycle biosynthetic process	4	5
GO:0019438	aromatic compound biosynthetic process	4	5
GO:0032774	RNA biosynthetic process	5	5
GO:0033683	obsolete nucleotide-excision repair, DNA incision	6	1
GO:0034641	cellular nitrogen compound metabolic process	3	5
GO:0034654	nucleobase-containing compound biosynthetic process	4	5
GO:0043170	macromolecule metabolic process	3	5
GO:0044237	cellular metabolic process	2	5
GO:0044238	primary metabolic process	2	5
GO:0044249	cellular biosynthetic process	3	5
GO:0044271	cellular nitrogen compound biosynthetic process	4	5
GO:0046483	heterocycle metabolic process	3	5
GO:0071704	organic substance metabolic process	2	5
GO:0090304	nucleic acid metabolic process	4	5
GO:0090305	nucleic acid phosphodiester bond hydrolysis	5	1
GO:1901360	organic cyclic compound metabolic process	3	5
GO:1901362	organic cyclic compound biosynthetic process	4	5
GO:1901576	organic substance biosynthetic process	3	5
GO:0006259	DNA metabolic process	4	4
GO:0006281	DNA repair	5	4
GO:0006289	nucleotide-excision repair	6	4
GO:0006950	response to stress	2	4
GO:0006974	DNA damage response	4	4
GO:0033554	cellular response to stress	3	4
GO:0044260	obsolete cellular macromolecule metabolic process	3	4
GO:0050896	response to stimulus	1	4
GO:0051716	cellular response to stimulus	2	4

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003676	nucleic acid binding	3	12
GO:0003677	DNA binding	4	12
GO:0003678	DNA helicase activity	3	12
GO:0003824	catalytic activity	1	12
GO:0004386	helicase activity	2	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0008094	ATP-dependent activity, acting on DNA	2	12
GO:0016787	hydrolase activity	2	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140097	catalytic activity, acting on DNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0140657	ATP-dependent activity	1	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:0043138	3'-5' DNA helicase activity	4	1
GO:0016462	pyrophosphatase activity	5	2
GO:0016817	hydrolase activity, acting on acid anhydrides	3	2
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	2
GO:0016887	ATP hydrolysis activity	7	2
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	225	229	PF00656	0.419
CLV_C14_Caspase3-7	294	298	PF00656	0.383
CLV_NRD_NRD_1	220	222	PF00675	0.414
CLV_NRD_NRD_1	36	38	PF00675	0.425
CLV_NRD_NRD_1	368	370	PF00675	0.320
CLV_NRD_NRD_1	589	591	PF00675	0.295
CLV_NRD_NRD_1	737	739	PF00675	0.588
CLV_NRD_NRD_1	741	743	PF00675	0.620
CLV_NRD_NRD_1	99	101	PF00675	0.356
CLV_PCSK_FUR_1	738	742	PF00082	0.657
CLV_PCSK_KEX2_1	220	222	PF00082	0.419
CLV_PCSK_KEX2_1	36	38	PF00082	0.447
CLV_PCSK_KEX2_1	370	372	PF00082	0.314
CLV_PCSK_KEX2_1	589	591	PF00082	0.295
CLV_PCSK_KEX2_1	739	741	PF00082	0.612
CLV_PCSK_KEX2_1	99	101	PF00082	0.356
CLV_PCSK_PC1ET2_1	370	372	PF00082	0.317
CLV_PCSK_PC1ET2_1	739	741	PF00082	0.631
CLV_PCSK_PC7_1	366	372	PF00082	0.356
CLV_PCSK_SKI1_1	14	18	PF00082	0.465
CLV_PCSK_SKI1_1	249	253	PF00082	0.419
CLV_PCSK_SKI1_1	43	47	PF00082	0.351
CLV_PCSK_SKI1_1	513	517	PF00082	0.383
CLV_PCSK_SKI1_1	633	637	PF00082	0.314
DEG_APCC_DBOX_1	399	407	PF00400	0.419
DEG_APCC_DBOX_1	42	50	PF00400	0.384
DEG_APCC_KENBOX_2	371	375	PF00400	0.419
DOC_CKS1_1	458	463	PF01111	0.301
DOC_CYCLIN_RxL_1	37	48	PF00134	0.157
DOC_CYCLIN_yCln2_LP_2	379	385	PF00134	0.314
DOC_MAPK_gen_1	259	267	PF00069	0.300
DOC_MAPK_gen_1	36	42	PF00069	0.438
DOC_MAPK_gen_1	479	489	PF00069	0.282
DOC_MAPK_gen_1	668	678	PF00069	0.721
DOC_MAPK_MEF2A_6	249	256	PF00069	0.299
DOC_MAPK_MEF2A_6	259	267	PF00069	0.299
DOC_MAPK_MEF2A_6	479	488	PF00069	0.335
DOC_MAPK_MEF2A_6	565	573	PF00069	0.299
DOC_PP1_RVXF_1	499	506	PF00149	0.337
DOC_PP2B_LxvP_1	190	193	PF13499	0.487
DOC_PP2B_LxvP_1	241	244	PF13499	0.370
DOC_PP2B_LxvP_1	287	290	PF13499	0.402
DOC_PP2B_LxvP_1	379	382	PF13499	0.419
DOC_PP2B_LxvP_1	531	534	PF13499	0.285
DOC_USP7_MATH_1	168	172	PF00917	0.514
DOC_USP7_MATH_1	355	359	PF00917	0.221
DOC_USP7_MATH_1	395	399	PF00917	0.419
DOC_USP7_MATH_1	651	655	PF00917	0.599
DOC_USP7_MATH_1	71	75	PF00917	0.316
DOC_USP7_MATH_1	773	777	PF00917	0.613
DOC_USP7_MATH_1	94	98	PF00917	0.445
DOC_USP7_UBL2_3	372	376	PF12436	0.419
DOC_USP7_UBL2_3	664	668	PF12436	0.702
DOC_WW_Pin1_4	148	153	PF00397	0.529
DOC_WW_Pin1_4	457	462	PF00397	0.288
DOC_WW_Pin1_4	62	67	PF00397	0.314
LIG_14-3-3_CanoR_1	36	40	PF00244	0.366
LIG_14-3-3_CanoR_1	43	53	PF00244	0.392
LIG_14-3-3_CanoR_1	766	772	PF00244	0.706
LIG_14-3-3_CanoR_1	787	795	PF00244	0.677
LIG_APCC_ABBA_1	153	158	PF00400	0.519
LIG_APCC_ABBA_1	546	551	PF00400	0.299
LIG_APCC_ABBAyCdc20_2	545	551	PF00400	0.299
LIG_BIR_III_4	300	304	PF00653	0.260
LIG_BIR_III_4	407	411	PF00653	0.333
LIG_BRCT_BRCA1_1	401	405	PF00533	0.384
LIG_Clathr_ClatBox_1	385	389	PF01394	0.299
LIG_Clathr_ClatBox_1	794	798	PF01394	0.582
LIG_EH_1	649	653	PF12763	0.280
LIG_FHA_1	206	212	PF00498	0.519
LIG_FHA_1	262	268	PF00498	0.299
LIG_FHA_1	329	335	PF00498	0.299
LIG_FHA_1	553	559	PF00498	0.299
LIG_FHA_1	632	638	PF00498	0.337
LIG_FHA_1	710	716	PF00498	0.534
LIG_FHA_1	81	87	PF00498	0.389
LIG_FHA_2	46	52	PF00498	0.333
LIG_Integrin_isoDGR_2	608	610	PF01839	0.445
LIG_LIR_Gen_1	24	35	PF02991	0.330
LIG_LIR_Gen_1	283	292	PF02991	0.299
LIG_LIR_Gen_1	336	344	PF02991	0.299
LIG_LIR_Gen_1	38	46	PF02991	0.275
LIG_LIR_Gen_1	427	437	PF02991	0.361
LIG_LIR_Gen_1	502	512	PF02991	0.219
LIG_LIR_Gen_1	681	692	PF02991	0.693
LIG_LIR_Gen_1	770	777	PF02991	0.660
LIG_LIR_LC3C_4	264	267	PF02991	0.402
LIG_LIR_Nem_3	24	30	PF02991	0.405
LIG_LIR_Nem_3	283	287	PF02991	0.299
LIG_LIR_Nem_3	336	341	PF02991	0.297
LIG_LIR_Nem_3	377	383	PF02991	0.305
LIG_LIR_Nem_3	38	42	PF02991	0.275
LIG_LIR_Nem_3	427	432	PF02991	0.364
LIG_LIR_Nem_3	502	508	PF02991	0.219
LIG_LIR_Nem_3	526	530	PF02991	0.297
LIG_LIR_Nem_3	613	619	PF02991	0.286
LIG_LIR_Nem_3	648	652	PF02991	0.401
LIG_LIR_Nem_3	681	687	PF02991	0.686
LIG_LIR_Nem_3	770	774	PF02991	0.656
LIG_Pex14_1	729	733	PF04695	0.450
LIG_SH2_CRK	338	342	PF00017	0.299
LIG_SH2_CRK	383	387	PF00017	0.314
LIG_SH2_CRK	617	621	PF00017	0.299
LIG_SH2_CRK	771	775	PF00017	0.656
LIG_SH2_NCK_1	617	621	PF00017	0.299
LIG_SH2_PTP2	27	30	PF00017	0.374
LIG_SH2_PTP2	39	42	PF00017	0.333
LIG_SH2_SRC	157	160	PF00017	0.499
LIG_SH2_SRC	39	42	PF00017	0.260
LIG_SH2_SRC	437	440	PF00017	0.513
LIG_SH2_SRC	89	92	PF00017	0.326
LIG_SH2_STAP1	338	342	PF00017	0.314
LIG_SH2_STAP1	437	441	PF00017	0.426
LIG_SH2_STAP1	642	646	PF00017	0.333
LIG_SH2_STAT5	27	30	PF00017	0.374
LIG_SH2_STAT5	39	42	PF00017	0.333
LIG_SH2_STAT5	418	421	PF00017	0.429
LIG_SH2_STAT5	449	452	PF00017	0.314
LIG_SH3_3	257	263	PF00018	0.314
LIG_SH3_3	455	461	PF00018	0.314
LIG_SH3_3	568	574	PF00018	0.299
LIG_SH3_3	689	695	PF00018	0.702
LIG_Sin3_3	483	490	PF02671	0.157
LIG_SUMO_SIM_anti_2	519	526	PF11976	0.299
LIG_SUMO_SIM_par_1	384	390	PF11976	0.419
LIG_TRFH_1	62	66	PF08558	0.419
LIG_TYR_ITIM	282	287	PF00017	0.384
LIG_UBA3_1	251	259	PF00899	0.325
LIG_UBA3_1	428	435	PF00899	0.373
LIG_UBA3_1	521	525	PF00899	0.318
LIG_WRC_WIRS_1	169	174	PF05994	0.479
MOD_CDK_SPxK_1	148	154	PF00069	0.473
MOD_CDK_SPxxK_3	457	464	PF00069	0.314
MOD_CK1_1	109	115	PF00069	0.426
MOD_CK1_1	123	129	PF00069	0.190
MOD_CK1_1	205	211	PF00069	0.472
MOD_CK1_1	212	218	PF00069	0.520
MOD_CK1_1	440	446	PF00069	0.402
MOD_CK1_1	54	60	PF00069	0.318
MOD_CK1_1	618	624	PF00069	0.302
MOD_CK1_1	655	661	PF00069	0.661
MOD_CK2_1	158	164	PF00069	0.571
MOD_CK2_1	17	23	PF00069	0.491
MOD_CK2_1	395	401	PF00069	0.218
MOD_CK2_1	45	51	PF00069	0.333
MOD_CK2_1	679	685	PF00069	0.659
MOD_CK2_1	724	730	PF00069	0.439
MOD_GlcNHglycan	23	26	PF01048	0.263
MOD_GlcNHglycan	237	240	PF01048	0.299
MOD_GlcNHglycan	5	8	PF01048	0.716
MOD_GlcNHglycan	696	699	PF01048	0.719
MOD_GlcNHglycan	700	703	PF01048	0.568
MOD_GlcNHglycan	757	760	PF01048	0.730
MOD_GlcNHglycan	783	786	PF01048	0.630
MOD_GSK3_1	144	151	PF00069	0.404
MOD_GSK3_1	17	24	PF00069	0.553
MOD_GSK3_1	173	180	PF00069	0.507
MOD_GSK3_1	201	208	PF00069	0.532
MOD_GSK3_1	31	38	PF00069	0.255
MOD_GSK3_1	336	343	PF00069	0.314
MOD_GSK3_1	351	358	PF00069	0.314
MOD_GSK3_1	395	402	PF00069	0.368
MOD_GSK3_1	547	554	PF00069	0.299
MOD_GSK3_1	581	588	PF00069	0.290
MOD_GSK3_1	651	658	PF00069	0.604
MOD_GSK3_1	679	686	PF00069	0.655
MOD_GSK3_1	694	701	PF00069	0.586
MOD_GSK3_1	709	716	PF00069	0.542
MOD_GSK3_1	720	727	PF00069	0.405
MOD_NEK2_1	1	6	PF00069	0.705
MOD_NEK2_1	106	111	PF00069	0.303
MOD_NEK2_1	17	22	PF00069	0.452
MOD_NEK2_1	173	178	PF00069	0.442
MOD_NEK2_1	340	345	PF00069	0.313
MOD_NEK2_1	35	40	PF00069	0.116
MOD_NEK2_1	399	404	PF00069	0.401
MOD_NEK2_1	414	419	PF00069	0.156
MOD_NEK2_1	45	50	PF00069	0.293
MOD_NEK2_1	471	476	PF00069	0.354
MOD_NEK2_1	552	557	PF00069	0.286
MOD_NEK2_1	652	657	PF00069	0.582
MOD_NEK2_1	683	688	PF00069	0.607
MOD_NEK2_1	85	90	PF00069	0.384
MOD_NEK2_2	209	214	PF00069	0.530
MOD_NEK2_2	395	400	PF00069	0.419
MOD_PIKK_1	212	218	PF00454	0.439
MOD_PIKK_1	276	282	PF00454	0.445
MOD_PIKK_1	443	449	PF00454	0.419
MOD_PIKK_1	537	543	PF00454	0.419
MOD_PK_1	202	208	PF00069	0.310
MOD_PKA_1	261	267	PF00069	0.299
MOD_PKA_1	740	746	PF00069	0.655
MOD_PKA_2	35	41	PF00069	0.345
MOD_PKA_2	399	405	PF00069	0.384
MOD_PKA_2	472	478	PF00069	0.351
MOD_PKA_2	54	60	PF00069	0.307
MOD_PKA_2	740	746	PF00069	0.668
MOD_PKA_2	786	792	PF00069	0.667
MOD_PKB_1	738	746	PF00069	0.668
MOD_Plk_1	123	129	PF00069	0.419
MOD_Plk_1	157	163	PF00069	0.493
MOD_Plk_1	202	208	PF00069	0.430
MOD_Plk_1	437	443	PF00069	0.376
MOD_Plk_1	567	573	PF00069	0.294
MOD_Plk_1	80	86	PF00069	0.419
MOD_Plk_2-3	158	164	PF00069	0.583
MOD_Plk_2-3	325	331	PF00069	0.299
MOD_Plk_2-3	8	14	PF00069	0.539
MOD_Plk_4	168	174	PF00069	0.489
MOD_Plk_4	247	253	PF00069	0.299
MOD_Plk_4	261	267	PF00069	0.299
MOD_Plk_4	336	342	PF00069	0.299
MOD_Plk_4	414	420	PF00069	0.332
MOD_Plk_4	54	60	PF00069	0.326
MOD_Plk_4	615	621	PF00069	0.299
MOD_Plk_4	679	685	PF00069	0.664
MOD_Plk_4	688	694	PF00069	0.700
MOD_ProDKin_1	148	154	PF00069	0.535
MOD_ProDKin_1	457	463	PF00069	0.288
MOD_ProDKin_1	62	68	PF00069	0.314
MOD_SUMO_for_1	678	681	PF00179	0.558
MOD_SUMO_for_1	774	777	PF00179	0.540
MOD_SUMO_rev_2	401	411	PF00179	0.330
MOD_SUMO_rev_2	421	426	PF00179	0.337
MOD_SUMO_rev_2	6	16	PF00179	0.333
TRG_DiLeu_BaEn_1	526	531	PF01217	0.299
TRG_DiLeu_BaEn_1	98	103	PF01217	0.383
TRG_DiLeu_BaEn_2	12	18	PF01217	0.483
TRG_DiLeu_BaEn_2	400	406	PF01217	0.274
TRG_DiLeu_BaEn_2	80	86	PF01217	0.356
TRG_DiLeu_BaEn_3	133	139	PF01217	0.419
TRG_DiLeu_BaLyEn_6	41	46	PF01217	0.219
TRG_ENDOCYTIC_2	169	172	PF00928	0.477
TRG_ENDOCYTIC_2	27	30	PF00928	0.374
TRG_ENDOCYTIC_2	284	287	PF00928	0.384
TRG_ENDOCYTIC_2	338	341	PF00928	0.299
TRG_ENDOCYTIC_2	383	386	PF00928	0.314
TRG_ENDOCYTIC_2	39	42	PF00928	0.333
TRG_ENDOCYTIC_2	527	530	PF00928	0.298
TRG_ENDOCYTIC_2	617	620	PF00928	0.299
TRG_ENDOCYTIC_2	649	652	PF00928	0.419
TRG_ENDOCYTIC_2	684	687	PF00928	0.693
TRG_ENDOCYTIC_2	771	774	PF00928	0.654
TRG_ENDOCYTIC_2	803	806	PF00928	0.580
TRG_ER_diArg_1	219	221	PF00400	0.359
TRG_ER_diArg_1	35	37	PF00400	0.425
TRG_ER_diArg_1	368	371	PF00400	0.321
TRG_ER_diArg_1	693	696	PF00400	0.630
TRG_ER_diArg_1	738	741	PF00400	0.652
TRG_NLS_MonoCore_2	737	742	PF00514	0.650
TRG_NLS_MonoExtC_3	737	742	PF00514	0.624
TRG_NLS_MonoExtN_4	738	743	PF00514	0.633
TRG_Pf-PMV_PEXEL_1	100	105	PF00026	0.389
TRG_Pf-PMV_PEXEL_1	118	122	PF00026	0.305
TRG_Pf-PMV_PEXEL_1	125	129	PF00026	0.335
TRG_Pf-PMV_PEXEL_1	501	506	PF00026	0.401

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IIS9	Leptomonas seymouri	76%	99%
A0A0S4J760	Bodo saltans	46%	75%
A0A1X0NS07	Trypanosomatidae	57%	100%
A0A3R7MM80	Trypanosoma rangeli	59%	100%
A0A3S7X6B2	Leishmania donovani	94%	100%
A4HH67	Leishmania braziliensis	29%	73%
A4HL29	Leishmania braziliensis	84%	100%
A4I8L0	Leishmania infantum	94%	100%
A6WE36	Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)	31%	100%
A9CRJ7	Enterocytozoon bieneusi (strain H348)	34%	100%
C4V922	Nosema ceranae (strain BRL01)	34%	100%
D0AAK6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	100%
O00835	Dictyostelium discoideum	33%	100%
O13768	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	34%	100%
O53873	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	29%	100%
P19447	Homo sapiens	34%	100%
P49135	Mus musculus	34%	100%
Q00578	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	34%	96%
Q1RMT1	Bos taurus	34%	100%
Q381F9	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	55%	100%
Q38861	Arabidopsis thaliana	33%	100%
Q4G005	Rattus norvegicus	34%	100%
Q4Q4N0	Leishmania major	93%	100%
Q5RA62	Pongo abelii	34%	100%
Q5ZKK7	Gallus gallus	34%	100%
Q60HG1	Macaca fascicularis	34%	100%
Q6E6J3	Antonospora locustae	35%	100%
Q7ZVV1	Danio rerio	33%	100%
Q8SSK1	Encephalitozoon cuniculi (strain GB-M1)	35%	100%
Q9FUG4	Arabidopsis thaliana	33%	100%
V5BDD7	Trypanosoma cruzi	58%	100%