tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Methyltransferase TYW3, putative

Species:

Leishmania mexicana

UniProt:

E9B3F3_LEIMU

TriTrypDb:

LmxM.31.3750

Length:

270

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B3F3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B3F3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0032259	methylation	2	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0001510	RNA methylation	4	1
GO:0006400	tRNA modification	6	1
GO:0009451	RNA modification	5	1
GO:0030488	tRNA methylation	5	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008168	methyltransferase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0008173	RNA methyltransferase activity	4	1
GO:0008175	tRNA methyltransferase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	188	190	PF00675	0.243
CLV_NRD_NRD_1	244	246	PF00675	0.285
CLV_NRD_NRD_1	265	267	PF00675	0.519
CLV_NRD_NRD_1	86	88	PF00675	0.265
CLV_PCSK_FUR_1	244	248	PF00082	0.386
CLV_PCSK_FUR_1	86	90	PF00082	0.326
CLV_PCSK_KEX2_1	244	246	PF00082	0.288
CLV_PCSK_KEX2_1	265	267	PF00082	0.544
CLV_PCSK_KEX2_1	86	88	PF00082	0.259
CLV_PCSK_PC1ET2_1	246	248	PF00082	0.324
CLV_PCSK_PC1ET2_1	88	90	PF00082	0.250
CLV_PCSK_SKI1_1	189	193	PF00082	0.270
CLV_PCSK_SKI1_1	49	53	PF00082	0.306
DEG_Nend_UBRbox_1	1	4	PF02207	0.278
DEG_SPOP_SBC_1	41	45	PF00917	0.250
DOC_MAPK_gen_1	244	252	PF00069	0.380
DOC_MAPK_MEF2A_6	145	153	PF00069	0.261
DOC_USP7_MATH_1	129	133	PF00917	0.363
DOC_USP7_MATH_1	73	77	PF00917	0.423
DOC_USP7_MATH_1	80	84	PF00917	0.227
DOC_WW_Pin1_4	211	216	PF00397	0.340
DOC_WW_Pin1_4	62	67	PF00397	0.442
DOC_WW_Pin1_4	74	79	PF00397	0.348
LIG_14-3-3_CanoR_1	127	136	PF00244	0.339
LIG_14-3-3_CanoR_1	189	198	PF00244	0.314
LIG_Clathr_ClatBox_1	198	202	PF01394	0.223
LIG_deltaCOP1_diTrp_1	121	126	PF00928	0.393
LIG_deltaCOP1_diTrp_1	209	218	PF00928	0.346
LIG_deltaCOP1_diTrp_1	221	226	PF00928	0.350
LIG_FHA_1	137	143	PF00498	0.281
LIG_FHA_1	190	196	PF00498	0.288
LIG_FHA_1	208	214	PF00498	0.431
LIG_FHA_1	226	232	PF00498	0.291
LIG_FHA_1	36	42	PF00498	0.414
LIG_FHA_2	109	115	PF00498	0.421
LIG_LIR_Gen_1	220	231	PF02991	0.393
LIG_LIR_Nem_3	220	226	PF02991	0.305
LIG_LIR_Nem_3	35	40	PF02991	0.277
LIG_MYND_3	117	121	PF01753	0.386
LIG_PTB_Apo_2	31	38	PF02174	0.265
LIG_SH2_GRB2like	32	35	PF00017	0.393
LIG_SH2_SRC	32	35	PF00017	0.393
LIG_SH2_STAT5	32	35	PF00017	0.313
LIG_SH2_STAT5	40	43	PF00017	0.251
LIG_SH3_3	198	204	PF00018	0.275
LIG_SH3_3	69	75	PF00018	0.245
LIG_TRAF2_1	119	122	PF00917	0.352
MOD_CK1_1	182	188	PF00069	0.284
MOD_CK1_1	35	41	PF00069	0.217
MOD_CK1_1	59	65	PF00069	0.409
MOD_CK1_1	76	82	PF00069	0.263
MOD_CK2_1	108	114	PF00069	0.331
MOD_CK2_1	79	85	PF00069	0.399
MOD_GlcNHglycan	170	173	PF01048	0.303
MOD_GlcNHglycan	175	178	PF01048	0.324
MOD_GSK3_1	108	115	PF00069	0.310
MOD_GSK3_1	173	180	PF00069	0.279
MOD_GSK3_1	189	196	PF00069	0.265
MOD_GSK3_1	207	214	PF00069	0.205
MOD_GSK3_1	236	243	PF00069	0.226
MOD_GSK3_1	35	42	PF00069	0.301
MOD_GSK3_1	4	11	PF00069	0.421
MOD_GSK3_1	57	64	PF00069	0.359
MOD_GSK3_1	76	83	PF00069	0.464
MOD_GSK3_1	93	100	PF00069	0.310
MOD_N-GLC_1	240	245	PF02516	0.278
MOD_N-GLC_1	33	38	PF02516	0.348
MOD_NEK2_1	227	232	PF00069	0.220
MOD_NEK2_2	93	98	PF00069	0.310
MOD_PIKK_1	35	41	PF00454	0.326
MOD_PKA_1	189	195	PF00069	0.243
MOD_PKA_1	88	94	PF00069	0.344
MOD_PKA_2	112	118	PF00069	0.254
MOD_PKA_2	182	188	PF00069	0.273
MOD_PKA_2	57	63	PF00069	0.205
MOD_PKA_2	88	94	PF00069	0.344
MOD_Plk_1	106	112	PF00069	0.393
MOD_Plk_1	14	20	PF00069	0.268
MOD_Plk_1	33	39	PF00069	0.183
MOD_Plk_4	112	118	PF00069	0.336
MOD_Plk_4	194	200	PF00069	0.370
MOD_Plk_4	222	228	PF00069	0.350
MOD_Plk_4	93	99	PF00069	0.326
MOD_ProDKin_1	211	217	PF00069	0.340
MOD_ProDKin_1	62	68	PF00069	0.442
MOD_ProDKin_1	74	80	PF00069	0.348
MOD_SUMO_for_1	144	147	PF00179	0.250
TRG_ER_diArg_1	244	247	PF00400	0.271
TRG_ER_diArg_1	86	89	PF00400	0.326
TRG_NLS_MonoExtN_4	86	91	PF00514	0.413
TRG_Pf-PMV_PEXEL_1	266	270	PF00026	0.423

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PB24	Leptomonas seymouri	69%	95%
A0A0S4JMK0	Bodo saltans	27%	83%
A0A1X0NRX8	Trypanosomatidae	53%	89%
A0A3R7NUS4	Trypanosoma rangeli	53%	98%
A0A3S7X653	Leishmania donovani	93%	100%
A4HL12	Leishmania braziliensis	79%	100%
A4I8J3	Leishmania infantum	92%	100%
D0AAM5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	91%
Q4Q4P7	Leishmania major	89%	100%
Q9UTA5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	100%
V5DMF2	Trypanosoma cruzi	53%	97%