TRUD domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase D (TruD), putative

Species:

Leishmania mexicana

UniProt:

E9B3C1_LEIMU

TriTrypDb:

LmxM.31.3380

Length:

719

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 8
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B3C1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B3C1

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	10
GO:0006139	nucleobase-containing compound metabolic process	3	10
GO:0006725	cellular aromatic compound metabolic process	3	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008152	metabolic process	1	10
GO:0009451	RNA modification	5	10
GO:0009987	cellular process	1	10
GO:0016070	RNA metabolic process	5	10
GO:0034641	cellular nitrogen compound metabolic process	3	10
GO:0043170	macromolecule metabolic process	3	10
GO:0043412	macromolecule modification	4	10
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	10
GO:0046483	heterocycle metabolic process	3	10
GO:0071704	organic substance metabolic process	2	10
GO:0090304	nucleic acid metabolic process	4	10
GO:1901360	organic cyclic compound metabolic process	3	10

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	10
GO:0003723	RNA binding	4	10
GO:0003824	catalytic activity	1	10
GO:0005488	binding	1	10
GO:0009982	pseudouridine synthase activity	4	10
GO:0016853	isomerase activity	2	10
GO:0016866	intramolecular transferase activity	3	10
GO:0097159	organic cyclic compound binding	2	10
GO:1901363	heterocyclic compound binding	2	10
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1
GO:0016829	lyase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	177	181	PF00656	0.640
CLV_NRD_NRD_1	123	125	PF00675	0.642
CLV_NRD_NRD_1	148	150	PF00675	0.419
CLV_NRD_NRD_1	156	158	PF00675	0.426
CLV_NRD_NRD_1	205	207	PF00675	0.666
CLV_NRD_NRD_1	239	241	PF00675	0.285
CLV_NRD_NRD_1	357	359	PF00675	0.299
CLV_NRD_NRD_1	389	391	PF00675	0.343
CLV_NRD_NRD_1	638	640	PF00675	0.441
CLV_NRD_NRD_1	673	675	PF00675	0.551
CLV_NRD_NRD_1	84	86	PF00675	0.392
CLV_PCSK_KEX2_1	123	125	PF00082	0.644
CLV_PCSK_KEX2_1	148	150	PF00082	0.419
CLV_PCSK_KEX2_1	156	158	PF00082	0.426
CLV_PCSK_KEX2_1	205	207	PF00082	0.666
CLV_PCSK_KEX2_1	241	243	PF00082	0.260
CLV_PCSK_KEX2_1	357	359	PF00082	0.343
CLV_PCSK_KEX2_1	389	391	PF00082	0.343
CLV_PCSK_KEX2_1	638	640	PF00082	0.563
CLV_PCSK_KEX2_1	672	674	PF00082	0.625
CLV_PCSK_PC1ET2_1	241	243	PF00082	0.260
CLV_PCSK_PC7_1	668	674	PF00082	0.383
CLV_PCSK_SKI1_1	250	254	PF00082	0.318
CLV_PCSK_SKI1_1	295	299	PF00082	0.356
CLV_PCSK_SKI1_1	384	388	PF00082	0.461
CLV_PCSK_SKI1_1	433	437	PF00082	0.175
CLV_PCSK_SKI1_1	500	504	PF00082	0.322
CLV_PCSK_SKI1_1	710	714	PF00082	0.333
CLV_PCSK_SKI1_1	85	89	PF00082	0.501
CLV_Separin_Metazoa	153	157	PF03568	0.483
DEG_APCC_DBOX_1	80	88	PF00400	0.490
DEG_MDM2_SWIB_1	424	432	PF02201	0.377
DOC_ANK_TNKS_1	122	129	PF00023	0.477
DOC_ANK_TNKS_1	175	182	PF00023	0.616
DOC_CYCLIN_RxL_1	292	300	PF00134	0.372
DOC_CYCLIN_yCln2_LP_2	309	315	PF00134	0.366
DOC_MAPK_gen_1	167	175	PF00069	0.321
DOC_MAPK_gen_1	270	277	PF00069	0.332
DOC_MAPK_gen_1	638	645	PF00069	0.372
DOC_MAPK_MEF2A_6	690	697	PF00069	0.352
DOC_MAPK_NFAT4_5	690	698	PF00069	0.281
DOC_MAPK_RevD_3	190	206	PF00069	0.463
DOC_PP1_RVXF_1	345	352	PF00149	0.377
DOC_PP1_RVXF_1	623	630	PF00149	0.443
DOC_PP4_FxxP_1	697	700	PF00568	0.302
DOC_USP7_MATH_1	127	131	PF00917	0.654
DOC_USP7_MATH_1	135	139	PF00917	0.636
DOC_USP7_MATH_1	140	144	PF00917	0.646
DOC_USP7_MATH_1	191	195	PF00917	0.603
DOC_USP7_MATH_1	315	319	PF00917	0.375
DOC_USP7_MATH_1	325	329	PF00917	0.370
DOC_USP7_MATH_1	431	435	PF00917	0.286
DOC_USP7_MATH_1	456	460	PF00917	0.157
DOC_USP7_MATH_1	481	485	PF00917	0.377
DOC_USP7_MATH_1	517	521	PF00917	0.329
DOC_USP7_MATH_1	555	559	PF00917	0.733
DOC_WW_Pin1_4	18	23	PF00397	0.574
DOC_WW_Pin1_4	216	221	PF00397	0.719
DOC_WW_Pin1_4	26	31	PF00397	0.516
DOC_WW_Pin1_4	359	364	PF00397	0.324
LIG_14-3-3_CanoR_1	170	174	PF00244	0.353
LIG_14-3-3_CanoR_1	193	202	PF00244	0.721
LIG_14-3-3_CanoR_1	279	283	PF00244	0.266
LIG_14-3-3_CanoR_1	295	304	PF00244	0.390
LIG_14-3-3_CanoR_1	324	334	PF00244	0.423
LIG_14-3-3_CanoR_1	407	415	PF00244	0.317
LIG_14-3-3_CanoR_1	470	474	PF00244	0.240
LIG_14-3-3_CanoR_1	62	66	PF00244	0.505
LIG_14-3-3_CanoR_1	628	635	PF00244	0.467
LIG_14-3-3_CanoR_1	71	77	PF00244	0.597
LIG_BIR_III_2	217	221	PF00653	0.610
LIG_BRCT_BRCA1_1	519	523	PF00533	0.260
LIG_deltaCOP1_diTrp_1	626	635	PF00928	0.420
LIG_FHA_1	221	227	PF00498	0.616
LIG_FHA_1	464	470	PF00498	0.380
LIG_FHA_1	501	507	PF00498	0.444
LIG_FHA_1	690	696	PF00498	0.494
LIG_FHA_1	704	710	PF00498	0.299
LIG_FHA_2	111	117	PF00498	0.512
LIG_FHA_2	132	138	PF00498	0.497
LIG_FHA_2	175	181	PF00498	0.666
LIG_FHA_2	205	211	PF00498	0.745
LIG_FHA_2	363	369	PF00498	0.391
LIG_LIR_Apic_2	696	700	PF02991	0.418
LIG_LIR_Gen_1	113	122	PF02991	0.517
LIG_LIR_Gen_1	318	327	PF02991	0.266
LIG_LIR_Gen_1	42	52	PF02991	0.369
LIG_LIR_Gen_1	491	502	PF02991	0.312
LIG_LIR_Gen_1	701	712	PF02991	0.308
LIG_LIR_Nem_3	113	118	PF02991	0.491
LIG_LIR_Nem_3	255	259	PF02991	0.347
LIG_LIR_Nem_3	318	322	PF02991	0.249
LIG_LIR_Nem_3	338	344	PF02991	0.372
LIG_LIR_Nem_3	396	402	PF02991	0.281
LIG_LIR_Nem_3	42	48	PF02991	0.414
LIG_LIR_Nem_3	472	476	PF02991	0.369
LIG_LIR_Nem_3	491	497	PF02991	0.312
LIG_LIR_Nem_3	520	525	PF02991	0.323
LIG_LIR_Nem_3	648	652	PF02991	0.453
LIG_LIR_Nem_3	701	707	PF02991	0.315
LIG_LIR_Nem_3	93	99	PF02991	0.508
LIG_LYPXL_S_1	158	162	PF13949	0.492
LIG_LYPXL_yS_3	159	162	PF13949	0.368
LIG_MLH1_MIPbox_1	519	523	PF16413	0.377
LIG_Pex14_2	399	403	PF04695	0.260
LIG_Pex14_2	424	428	PF04695	0.370
LIG_SH2_CRK	319	323	PF00017	0.377
LIG_SH2_CRK	704	708	PF00017	0.295
LIG_SH2_NCK_1	704	708	PF00017	0.295
LIG_SH2_PTP2	234	237	PF00017	0.332
LIG_SH2_SRC	234	237	PF00017	0.332
LIG_SH2_STAP1	319	323	PF00017	0.377
LIG_SH2_STAP1	704	708	PF00017	0.295
LIG_SH2_STAT3	402	405	PF00017	0.260
LIG_SH2_STAT5	234	237	PF00017	0.461
LIG_SH2_STAT5	256	259	PF00017	0.320
LIG_SH2_STAT5	402	405	PF00017	0.260
LIG_SH2_STAT5	45	48	PF00017	0.392
LIG_SH2_STAT5	476	479	PF00017	0.380
LIG_SH2_STAT5	509	512	PF00017	0.377
LIG_SH2_STAT5	529	532	PF00017	0.448
LIG_SH3_3	16	22	PF00018	0.681
LIG_SH3_3	309	315	PF00018	0.269
LIG_SH3_3	569	575	PF00018	0.473
LIG_SH3_3	640	646	PF00018	0.372
LIG_SH3_3	650	656	PF00018	0.323
MOD_CDK_SPK_2	18	23	PF00069	0.527
MOD_CK1_1	114	120	PF00069	0.623
MOD_CK1_1	194	200	PF00069	0.579
MOD_CK1_1	219	225	PF00069	0.675
MOD_CK1_1	326	332	PF00069	0.342
MOD_CK1_1	35	41	PF00069	0.635
MOD_CK1_1	362	368	PF00069	0.286
MOD_CK1_1	547	553	PF00069	0.548
MOD_CK1_1	557	563	PF00069	0.568
MOD_CK1_1	564	570	PF00069	0.714
MOD_CK1_1	90	96	PF00069	0.604
MOD_CK2_1	114	120	PF00069	0.520
MOD_CK2_1	131	137	PF00069	0.440
MOD_CK2_1	204	210	PF00069	0.730
MOD_CK2_1	362	368	PF00069	0.394
MOD_CK2_1	564	570	PF00069	0.598
MOD_CMANNOS	612	615	PF00535	0.456
MOD_GlcNHglycan	129	132	PF01048	0.449
MOD_GlcNHglycan	196	199	PF01048	0.713
MOD_GlcNHglycan	214	217	PF01048	0.715
MOD_GlcNHglycan	228	231	PF01048	0.582
MOD_GlcNHglycan	261	264	PF01048	0.370
MOD_GlcNHglycan	299	302	PF01048	0.267
MOD_GlcNHglycan	325	328	PF01048	0.301
MOD_GlcNHglycan	336	340	PF01048	0.293
MOD_GlcNHglycan	483	486	PF01048	0.380
MOD_GlcNHglycan	546	549	PF01048	0.756
MOD_GlcNHglycan	557	560	PF01048	0.666
MOD_GlcNHglycan	563	566	PF01048	0.593
MOD_GlcNHglycan	582	585	PF01048	0.314
MOD_GlcNHglycan	603	606	PF01048	0.610
MOD_GlcNHglycan	630	633	PF01048	0.499
MOD_GlcNHglycan	682	686	PF01048	0.518
MOD_GSK3_1	110	117	PF00069	0.620
MOD_GSK3_1	127	134	PF00069	0.451
MOD_GSK3_1	212	219	PF00069	0.576
MOD_GSK3_1	220	227	PF00069	0.590
MOD_GSK3_1	488	495	PF00069	0.375
MOD_GSK3_1	525	532	PF00069	0.394
MOD_GSK3_1	555	562	PF00069	0.728
MOD_GSK3_1	597	604	PF00069	0.539
MOD_GSK3_1	689	696	PF00069	0.388
MOD_GSK3_1	699	706	PF00069	0.330
MOD_N-GLC_1	182	187	PF02516	0.641
MOD_N-GLC_1	32	37	PF02516	0.569
MOD_N-GLC_1	488	493	PF02516	0.286
MOD_NEK2_1	110	115	PF00069	0.577
MOD_NEK2_1	297	302	PF00069	0.386
MOD_NEK2_1	323	328	PF00069	0.314
MOD_NEK2_1	469	474	PF00069	0.245
MOD_NEK2_1	525	530	PF00069	0.295
MOD_NEK2_1	627	632	PF00069	0.415
MOD_NEK2_2	169	174	PF00069	0.486
MOD_NEK2_2	191	196	PF00069	0.460
MOD_NEK2_2	431	436	PF00069	0.377
MOD_NEK2_2	517	522	PF00069	0.383
MOD_NEK2_2	703	708	PF00069	0.412
MOD_PK_1	273	279	PF00069	0.249
MOD_PKA_2	169	175	PF00069	0.389
MOD_PKA_2	204	210	PF00069	0.659
MOD_PKA_2	278	284	PF00069	0.260
MOD_PKA_2	323	329	PF00069	0.432
MOD_PKA_2	406	412	PF00069	0.311
MOD_PKA_2	469	475	PF00069	0.345
MOD_PKA_2	61	67	PF00069	0.540
MOD_PKA_2	627	633	PF00069	0.467
MOD_PKA_2	654	660	PF00069	0.452
MOD_PKA_2	689	695	PF00069	0.442
MOD_PKB_1	672	680	PF00069	0.406
MOD_Plk_1	182	188	PF00069	0.592
MOD_Plk_1	32	38	PF00069	0.567
MOD_Plk_1	598	604	PF00069	0.479
MOD_Plk_1	657	663	PF00069	0.571
MOD_Plk_4	32	38	PF00069	0.621
MOD_Plk_4	517	523	PF00069	0.301
MOD_Plk_4	525	531	PF00069	0.306
MOD_Plk_4	606	612	PF00069	0.277
MOD_Plk_4	657	663	PF00069	0.352
MOD_Plk_4	703	709	PF00069	0.338
MOD_ProDKin_1	18	24	PF00069	0.577
MOD_ProDKin_1	216	222	PF00069	0.718
MOD_ProDKin_1	26	32	PF00069	0.513
MOD_ProDKin_1	359	365	PF00069	0.324
TRG_DiLeu_BaEn_1	248	253	PF01217	0.260
TRG_DiLeu_BaEn_2	381	387	PF01217	0.264
TRG_DiLeu_BaEn_4	378	384	PF01217	0.217
TRG_ENDOCYTIC_2	159	162	PF00928	0.368
TRG_ENDOCYTIC_2	234	237	PF00928	0.332
TRG_ENDOCYTIC_2	256	259	PF00928	0.320
TRG_ENDOCYTIC_2	319	322	PF00928	0.264
TRG_ENDOCYTIC_2	443	446	PF00928	0.260
TRG_ENDOCYTIC_2	45	48	PF00928	0.349
TRG_ENDOCYTIC_2	704	707	PF00928	0.341
TRG_ER_diArg_1	122	124	PF00400	0.637
TRG_ER_diArg_1	147	149	PF00400	0.427
TRG_ER_diArg_1	155	157	PF00400	0.400
TRG_ER_diArg_1	175	178	PF00400	0.366
TRG_ER_diArg_1	205	208	PF00400	0.607
TRG_ER_diArg_1	239	242	PF00400	0.285
TRG_ER_diArg_1	344	347	PF00400	0.324
TRG_ER_diArg_1	357	359	PF00400	0.348
TRG_ER_diArg_1	389	391	PF00400	0.313
TRG_ER_diArg_1	638	640	PF00400	0.558
TRG_ER_diArg_1	672	674	PF00400	0.474
TRG_NES_CRM1_1	248	261	PF08389	0.285
TRG_NLS_MonoExtC_3	239	245	PF00514	0.260
TRG_Pf-PMV_PEXEL_1	250	255	PF00026	0.260
TRG_Pf-PMV_PEXEL_1	74	78	PF00026	0.571

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4F4	Leptomonas seymouri	49%	75%
A0A1X0NRV3	Trypanosomatidae	33%	100%
A0A3R7LD82	Trypanosoma rangeli	33%	100%
A0A3S7X658	Leishmania donovani	88%	100%
A4HKY3	Leishmania braziliensis	73%	100%
A4I8G2	Leishmania infantum	88%	77%
D0AAQ9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
Q08647	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	21%	100%
Q4Q4T4	Leishmania major	86%	98%