Tubulin-tyrosine ligase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Protein of unknown function (DUF2946), putative

Species:

Leishmania mexicana

UniProt:

E9B373_LEIMU

TriTrypDb:

Length:

495

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005874	microtubule	6	1
GO:0005876	spindle microtubule	7	1
GO:0099080	supramolecular complex	2	1
GO:0099081	supramolecular polymer	3	1
GO:0099512	supramolecular fiber	4	1
GO:0099513	polymeric cytoskeletal fiber	5	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B373

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B373

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008152	metabolic process	1	9
GO:0019538	protein metabolic process	3	9
GO:0036211	protein modification process	4	9
GO:0043170	macromolecule metabolic process	3	9
GO:0043412	macromolecule modification	4	9
GO:0044238	primary metabolic process	2	9
GO:0071704	organic substance metabolic process	2	9
GO:1901564	organonitrogen compound metabolic process	3	9
GO:0000226	microtubule cytoskeleton organization	3	1
GO:0006996	organelle organization	4	1
GO:0007010	cytoskeleton organization	5	1
GO:0007017	microtubule-based process	2	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0004835	tubulin-tyrosine ligase activity	3	6
GO:0016874	ligase activity	2	9
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	6
GO:0016881	acid-amino acid ligase activity	4	6
GO:0140096	catalytic activity, acting on a protein	2	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	313	317	PF00656	0.473
CLV_NRD_NRD_1	239	241	PF00675	0.248
CLV_NRD_NRD_1	273	275	PF00675	0.178
CLV_NRD_NRD_1	66	68	PF00675	0.402
CLV_NRD_NRD_1	84	86	PF00675	0.578
CLV_PCSK_KEX2_1	239	241	PF00082	0.271
CLV_PCSK_KEX2_1	66	68	PF00082	0.402
CLV_PCSK_KEX2_1	71	73	PF00082	0.455
CLV_PCSK_KEX2_1	84	86	PF00082	0.578
CLV_PCSK_PC1ET2_1	239	241	PF00082	0.337
CLV_PCSK_PC1ET2_1	71	73	PF00082	0.549
CLV_PCSK_PC7_1	67	73	PF00082	0.520
CLV_PCSK_SKI1_1	120	124	PF00082	0.357
CLV_PCSK_SKI1_1	13	17	PF00082	0.514
CLV_PCSK_SKI1_1	207	211	PF00082	0.322
CLV_PCSK_SKI1_1	233	237	PF00082	0.273
CLV_PCSK_SKI1_1	275	279	PF00082	0.201
CLV_PCSK_SKI1_1	326	330	PF00082	0.279
CLV_PCSK_SKI1_1	460	464	PF00082	0.480
DEG_APCC_DBOX_1	206	214	PF00400	0.495
DEG_APCC_DBOX_1	311	319	PF00400	0.473
DEG_Nend_UBRbox_1	1	4	PF02207	0.421
DEG_SCF_FBW7_1	153	158	PF00400	0.463
DOC_CYCLIN_RxL_1	10	18	PF00134	0.497
DOC_CYCLIN_RxL_1	207	218	PF00134	0.519
DOC_CYCLIN_yClb5_NLxxxL_5	437	446	PF00134	0.338
DOC_CYCLIN_yClb5_NLxxxL_5	44	53	PF00134	0.355
DOC_MAPK_gen_1	320	327	PF00069	0.401
DOC_MAPK_MEF2A_6	178	187	PF00069	0.378
DOC_MAPK_MEF2A_6	465	472	PF00069	0.438
DOC_MAPK_MEF2A_6	91	100	PF00069	0.665
DOC_MAPK_NFAT4_5	465	473	PF00069	0.440
DOC_PP1_RVXF_1	109	116	PF00149	0.483
DOC_PP1_RVXF_1	209	216	PF00149	0.516
DOC_PP1_SILK_1	207	212	PF00149	0.495
DOC_PP2B_LxvP_1	98	101	PF13499	0.625
DOC_USP7_MATH_1	101	105	PF00917	0.461
DOC_USP7_MATH_1	155	159	PF00917	0.526
DOC_USP7_MATH_1	188	192	PF00917	0.456
DOC_USP7_MATH_1	412	416	PF00917	0.458
DOC_USP7_MATH_1	83	87	PF00917	0.581
DOC_WW_Pin1_4	151	156	PF00397	0.463
DOC_WW_Pin1_4	278	283	PF00397	0.544
DOC_WW_Pin1_4	337	342	PF00397	0.536
DOC_WW_Pin1_4	361	366	PF00397	0.494
DOC_WW_Pin1_4	92	97	PF00397	0.638
LIG_14-3-3_CanoR_1	120	125	PF00244	0.354
LIG_14-3-3_CanoR_1	20	26	PF00244	0.491
LIG_14-3-3_CanoR_1	266	271	PF00244	0.448
LIG_14-3-3_CanoR_1	27	32	PF00244	0.519
LIG_14-3-3_CanoR_1	274	282	PF00244	0.448
LIG_14-3-3_CanoR_1	84	89	PF00244	0.604
LIG_14-3-3_CanoR_1	91	96	PF00244	0.613
LIG_Actin_WH2_2	109	127	PF00022	0.373
LIG_APCC_ABBA_1	257	262	PF00400	0.473
LIG_CaM_NSCaTE_8	445	452	PF13499	0.385
LIG_eIF4E_1	230	236	PF01652	0.448
LIG_eIF4E_1	258	264	PF01652	0.448
LIG_FHA_1	117	123	PF00498	0.414
LIG_FHA_1	128	134	PF00498	0.516
LIG_FHA_1	265	271	PF00498	0.497
LIG_FHA_1	30	36	PF00498	0.565
LIG_FHA_1	322	328	PF00498	0.448
LIG_FHA_1	389	395	PF00498	0.484
LIG_FHA_1	426	432	PF00498	0.471
LIG_FHA_1	465	471	PF00498	0.393
LIG_FHA_1	477	483	PF00498	0.570
LIG_FHA_2	140	146	PF00498	0.466
LIG_FHA_2	38	44	PF00498	0.560
LIG_Integrin_RGD_1	203	205	PF01839	0.264
LIG_Integrin_RGD_1	348	350	PF01839	0.264
LIG_Integrin_RGD_1	373	375	PF01839	0.216
LIG_LIR_Apic_2	90	96	PF02991	0.587
LIG_LIR_Gen_1	18	26	PF02991	0.502
LIG_LIR_Gen_1	333	343	PF02991	0.378
LIG_LIR_Gen_1	426	437	PF02991	0.374
LIG_LIR_Gen_1	6	16	PF02991	0.441
LIG_LIR_LC3C_4	32	37	PF02991	0.523
LIG_LIR_Nem_3	18	24	PF02991	0.474
LIG_LIR_Nem_3	205	209	PF02991	0.566
LIG_LIR_Nem_3	253	258	PF02991	0.479
LIG_LIR_Nem_3	298	304	PF02991	0.458
LIG_LIR_Nem_3	333	338	PF02991	0.463
LIG_LIR_Nem_3	426	432	PF02991	0.394
LIG_NRBOX	48	54	PF00104	0.321
LIG_Pex14_2	432	436	PF04695	0.340
LIG_SH2_CRK	206	210	PF00017	0.495
LIG_SH2_CRK	335	339	PF00017	0.463
LIG_SH2_CRK	434	438	PF00017	0.297
LIG_SH2_CRK	93	97	PF00017	0.582
LIG_SH2_GRB2like	135	138	PF00017	0.409
LIG_SH2_GRB2like	357	360	PF00017	0.463
LIG_SH2_NCK_1	93	97	PF00017	0.582
LIG_SH2_PTP2	490	493	PF00017	0.362
LIG_SH2_SRC	304	307	PF00017	0.516
LIG_SH2_STAP1	255	259	PF00017	0.448
LIG_SH2_STAP1	335	339	PF00017	0.378
LIG_SH2_STAP1	434	438	PF00017	0.308
LIG_SH2_STAP1	8	12	PF00017	0.453
LIG_SH2_STAT5	146	149	PF00017	0.448
LIG_SH2_STAT5	230	233	PF00017	0.448
LIG_SH2_STAT5	258	261	PF00017	0.448
LIG_SH2_STAT5	335	338	PF00017	0.463
LIG_SH2_STAT5	475	478	PF00017	0.617
LIG_SH2_STAT5	490	493	PF00017	0.207
LIG_SH2_STAT5	93	96	PF00017	0.581
LIG_SH3_2	343	348	PF14604	0.463
LIG_SH3_2	453	458	PF14604	0.498
LIG_SH3_3	228	234	PF00018	0.495
LIG_SH3_3	340	346	PF00018	0.499
LIG_SH3_3	348	354	PF00018	0.463
LIG_SH3_3	415	421	PF00018	0.551
LIG_SH3_3	450	456	PF00018	0.473
LIG_SH3_3	471	477	PF00018	0.372
LIG_SUMO_SIM_par_1	390	395	PF11976	0.498
LIG_TRFH_1	151	155	PF08558	0.516
MOD_CDK_SPK_2	337	342	PF00069	0.499
MOD_CDK_SPxxK_3	278	285	PF00069	0.495
MOD_CDK_SPxxK_3	92	99	PF00069	0.641
MOD_CK1_1	127	133	PF00069	0.448
MOD_CK1_1	22	28	PF00069	0.687
MOD_CK1_1	337	343	PF00069	0.439
MOD_CK1_1	358	364	PF00069	0.515
MOD_CK1_1	87	93	PF00069	0.680
MOD_CK2_1	156	162	PF00069	0.595
MOD_CK2_1	299	305	PF00069	0.451
MOD_CK2_1	3	9	PF00069	0.416
MOD_GlcNHglycan	103	106	PF01048	0.517
MOD_GlcNHglycan	190	193	PF01048	0.285
MOD_GlcNHglycan	375	378	PF01048	0.264
MOD_GlcNHglycan	383	386	PF01048	0.244
MOD_GSK3_1	116	123	PF00069	0.368
MOD_GSK3_1	147	154	PF00069	0.463
MOD_GSK3_1	15	22	PF00069	0.567
MOD_GSK3_1	166	173	PF00069	0.371
MOD_GSK3_1	215	222	PF00069	0.483
MOD_GSK3_1	295	302	PF00069	0.458
MOD_GSK3_1	333	340	PF00069	0.463
MOD_GSK3_1	83	90	PF00069	0.638
MOD_N-GLC_1	358	363	PF02516	0.271
MOD_N-GLC_2	291	293	PF02516	0.248
MOD_NEK2_1	124	129	PF00069	0.448
MOD_NEK2_1	15	20	PF00069	0.526
MOD_NEK2_1	166	171	PF00069	0.459
MOD_NEK2_1	215	220	PF00069	0.529
MOD_NEK2_1	265	270	PF00069	0.461
MOD_NEK2_1	381	386	PF00069	0.378
MOD_NEK2_1	44	49	PF00069	0.683
MOD_NEK2_2	321	326	PF00069	0.495
MOD_PIKK_1	215	221	PF00454	0.473
MOD_PIKK_1	37	43	PF00454	0.585
MOD_PK_1	266	272	PF00069	0.448
MOD_PK_1	27	33	PF00069	0.550
MOD_PKA_1	84	90	PF00069	0.480
MOD_PKA_2	19	25	PF00069	0.571
MOD_PKA_2	265	271	PF00069	0.448
MOD_PKA_2	273	279	PF00069	0.448
MOD_PKA_2	355	361	PF00069	0.513
MOD_PKA_2	406	412	PF00069	0.510
MOD_PKA_2	83	89	PF00069	0.615
MOD_Plk_4	124	130	PF00069	0.468
MOD_Plk_4	299	305	PF00069	0.478
MOD_Plk_4	3	9	PF00069	0.459
MOD_Plk_4	334	340	PF00069	0.378
MOD_Plk_4	406	412	PF00069	0.498
MOD_ProDKin_1	151	157	PF00069	0.463
MOD_ProDKin_1	278	284	PF00069	0.544
MOD_ProDKin_1	337	343	PF00069	0.536
MOD_ProDKin_1	361	367	PF00069	0.494
MOD_ProDKin_1	92	98	PF00069	0.638
MOD_SUMO_for_1	330	333	PF00179	0.532
TRG_DiLeu_BaEn_1	162	167	PF01217	0.473
TRG_DiLeu_BaEn_4	305	311	PF01217	0.448
TRG_DiLeu_BaLyEn_6	231	236	PF01217	0.448
TRG_ENDOCYTIC_2	146	149	PF00928	0.473
TRG_ENDOCYTIC_2	206	209	PF00928	0.572
TRG_ENDOCYTIC_2	335	338	PF00928	0.463
TRG_ENDOCYTIC_2	434	437	PF00928	0.337
TRG_ENDOCYTIC_2	490	493	PF00928	0.328
TRG_ENDOCYTIC_2	8	11	PF00928	0.410
TRG_ER_diArg_1	66	68	PF00400	0.404
TRG_ER_diArg_1	73	76	PF00400	0.469
TRG_NES_CRM1_1	164	179	PF08389	0.425
TRG_Pf-PMV_PEXEL_1	42	46	PF00026	0.451

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8M9	Leptomonas seymouri	69%	77%
A0A1X0NRN8	Trypanosomatidae	42%	100%
A0A3Q8IH06	Leishmania donovani	95%	78%
A0A422NZN7	Trypanosoma rangeli	41%	96%
A4HKT5	Leishmania braziliensis	84%	100%
A4I8B4	Leishmania infantum	95%	100%
Q4Q4Y1	Leishmania major	92%	100%