DNA polymerase alpha subunit B

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA polymerase alpha/epsilon subunit B, putative

Species:

Leishmania mexicana

UniProt:

E9B2U8_LEIMU

TriTrypDb:

LmxM.31.1720

Length:

726

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	11
GO:0043226	organelle	2	11
GO:0043227	membrane-bounded organelle	3	11
GO:0043229	intracellular organelle	3	11
GO:0043231	intracellular membrane-bounded organelle	4	11
GO:0110165	cellular anatomical entity	1	11
GO:0000428	DNA-directed RNA polymerase complex	4	1
GO:0005658	alpha DNA polymerase:primase complex	4	1
GO:0030880	RNA polymerase complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0042575	DNA polymerase complex	3	1
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	1
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	1
GO:0140513	nuclear protein-containing complex	2	1
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990234	transferase complex	3	1

Expansion

Sequence features

E9B2U8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B2U8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006260	DNA replication	5	11
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006270	DNA replication initiation	5	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003677	DNA binding	4	12
GO:0005488	binding	1	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003824	catalytic activity	1	1
GO:0016740	transferase activity	2	1
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	1
GO:0016779	nucleotidyltransferase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	113	117	PF00656	0.647
CLV_C14_Caspase3-7	559	563	PF00656	0.667
CLV_C14_Caspase3-7	611	615	PF00656	0.701
CLV_C14_Caspase3-7	658	662	PF00656	0.642
CLV_C14_Caspase3-7	666	670	PF00656	0.485
CLV_NRD_NRD_1	343	345	PF00675	0.300
CLV_NRD_NRD_1	492	494	PF00675	0.281
CLV_NRD_NRD_1	707	709	PF00675	0.462
CLV_NRD_NRD_1	71	73	PF00675	0.450
CLV_PCSK_KEX2_1	343	345	PF00082	0.300
CLV_PCSK_KEX2_1	492	494	PF00082	0.281
CLV_PCSK_KEX2_1	599	601	PF00082	0.587
CLV_PCSK_KEX2_1	707	709	PF00082	0.462
CLV_PCSK_KEX2_1	71	73	PF00082	0.447
CLV_PCSK_PC1ET2_1	599	601	PF00082	0.587
CLV_PCSK_SKI1_1	28	32	PF00082	0.662
CLV_PCSK_SKI1_1	283	287	PF00082	0.516
CLV_PCSK_SKI1_1	308	312	PF00082	0.677
CLV_PCSK_SKI1_1	351	355	PF00082	0.322
CLV_PCSK_SKI1_1	381	385	PF00082	0.325
CLV_PCSK_SKI1_1	413	417	PF00082	0.321
CLV_PCSK_SKI1_1	420	424	PF00082	0.239
CLV_PCSK_SKI1_1	599	603	PF00082	0.579
CLV_PCSK_SKI1_1	708	712	PF00082	0.469
CLV_PCSK_SKI1_1	79	83	PF00082	0.330
DEG_APCC_DBOX_1	282	290	PF00400	0.508
DEG_APCC_DBOX_1	380	388	PF00400	0.411
DEG_APCC_DBOX_1	412	420	PF00400	0.373
DEG_Nend_UBRbox_3	1	3	PF02207	0.620
DEG_SPOP_SBC_1	315	319	PF00917	0.536
DEG_SPOP_SBC_1	563	567	PF00917	0.593
DOC_CDC14_PxL_1	517	525	PF14671	0.518
DOC_CKS1_1	30	35	PF01111	0.594
DOC_CYCLIN_RxL_1	25	32	PF00134	0.508
DOC_CYCLIN_RxL_1	378	386	PF00134	0.302
DOC_CYCLIN_RxL_1	417	425	PF00134	0.411
DOC_MAPK_DCC_7	633	642	PF00069	0.438
DOC_MAPK_gen_1	595	603	PF00069	0.568
DOC_MAPK_gen_1	71	77	PF00069	0.358
DOC_MAPK_MEF2A_6	518	525	PF00069	0.523
DOC_MAPK_MEF2A_6	633	642	PF00069	0.438
DOC_MAPK_MEF2A_6	8	17	PF00069	0.512
DOC_MAPK_RevD_3	692	708	PF00069	0.442
DOC_PP1_RVXF_1	331	337	PF00149	0.342
DOC_PP2B_LxvP_1	503	506	PF13499	0.300
DOC_PP4_FxxP_1	294	297	PF00568	0.347
DOC_PP4_FxxP_1	371	374	PF00568	0.442
DOC_USP7_MATH_1	126	130	PF00917	0.537
DOC_USP7_MATH_1	203	207	PF00917	0.753
DOC_USP7_MATH_1	214	218	PF00917	0.720
DOC_USP7_MATH_1	221	225	PF00917	0.738
DOC_USP7_MATH_1	325	329	PF00917	0.614
DOC_USP7_MATH_1	374	378	PF00917	0.425
DOC_USP7_MATH_1	38	42	PF00917	0.598
DOC_USP7_MATH_1	487	491	PF00917	0.366
DOC_USP7_MATH_1	543	547	PF00917	0.634
DOC_USP7_MATH_1	563	567	PF00917	0.647
DOC_USP7_MATH_1	570	574	PF00917	0.557
DOC_USP7_MATH_1	628	632	PF00917	0.587
DOC_USP7_UBL2_3	595	599	PF12436	0.462
DOC_WW_Pin1_4	230	235	PF00397	0.527
DOC_WW_Pin1_4	29	34	PF00397	0.605
DOC_WW_Pin1_4	615	620	PF00397	0.732
DOC_WW_Pin1_4	624	629	PF00397	0.755
DOC_WW_Pin1_4	684	689	PF00397	0.582
LIG_14-3-3_CanoR_1	251	255	PF00244	0.358
LIG_14-3-3_CanoR_1	257	262	PF00244	0.390
LIG_14-3-3_CanoR_1	351	358	PF00244	0.421
LIG_14-3-3_CanoR_1	413	423	PF00244	0.167
LIG_14-3-3_CanoR_1	457	461	PF00244	0.300
LIG_14-3-3_CanoR_1	55	60	PF00244	0.513
LIG_14-3-3_CanoR_1	707	716	PF00244	0.432
LIG_AP2alpha_2	276	278	PF02296	0.499
LIG_BIR_III_4	114	118	PF00653	0.588
LIG_BIR_III_4	605	609	PF00653	0.612
LIG_BRCT_BRCA1_1	337	341	PF00533	0.266
LIG_BRCT_BRCA1_1	686	690	PF00533	0.564
LIG_Clathr_ClatBox_1	16	20	PF01394	0.576
LIG_Clathr_ClatBox_1	43	47	PF01394	0.551
LIG_DLG_GKlike_1	55	62	PF00625	0.505
LIG_eIF4E_1	410	416	PF01652	0.411
LIG_FHA_1	221	227	PF00498	0.605
LIG_FHA_1	285	291	PF00498	0.319
LIG_FHA_1	410	416	PF00498	0.416
LIG_FHA_1	444	450	PF00498	0.330
LIG_FHA_1	512	518	PF00498	0.517
LIG_FHA_1	632	638	PF00498	0.681
LIG_FHA_1	700	706	PF00498	0.534
LIG_FHA_2	101	107	PF00498	0.696
LIG_FHA_2	234	240	PF00498	0.500
LIG_FHA_2	388	394	PF00498	0.279
LIG_FHA_2	399	405	PF00498	0.266
LIG_FHA_2	511	517	PF00498	0.423
LIG_FHA_2	616	622	PF00498	0.580
LIG_FHA_2	92	98	PF00498	0.654
LIG_Integrin_isoDGR_2	500	502	PF01839	0.331
LIG_Integrin_RGD_1	427	429	PF01839	0.287
LIG_Integrin_RGD_1	560	562	PF01839	0.595
LIG_LIR_Apic_2	293	297	PF02991	0.486
LIG_LIR_Apic_2	80	86	PF02991	0.229
LIG_LIR_Gen_1	390	399	PF02991	0.325
LIG_LIR_Gen_1	573	583	PF02991	0.566
LIG_LIR_LC3C_4	672	677	PF02991	0.431
LIG_LIR_Nem_3	159	163	PF02991	0.373
LIG_LIR_Nem_3	186	192	PF02991	0.613
LIG_LIR_Nem_3	260	265	PF02991	0.388
LIG_LIR_Nem_3	390	394	PF02991	0.386
LIG_LIR_Nem_3	56	62	PF02991	0.607
LIG_LIR_Nem_3	573	579	PF02991	0.569
LIG_MYND_1	29	33	PF01753	0.682
LIG_NRBOX	12	18	PF00104	0.525
LIG_PALB2_WD40_1	257	265	PF16756	0.377
LIG_PDZ_Class_2	721	726	PF00595	0.539
LIG_Pex14_1	576	580	PF04695	0.677
LIG_Pex14_2	371	375	PF04695	0.411
LIG_SH2_CRK	508	512	PF00017	0.296
LIG_SH2_CRK	59	63	PF00017	0.601
LIG_SH2_CRK	83	87	PF00017	0.243
LIG_SH2_NCK_1	441	445	PF00017	0.411
LIG_SH2_PTP2	504	507	PF00017	0.287
LIG_SH2_SRC	441	444	PF00017	0.411
LIG_SH2_SRC	527	530	PF00017	0.614
LIG_SH2_STAP1	255	259	PF00017	0.396
LIG_SH2_STAT3	255	258	PF00017	0.516
LIG_SH2_STAT5	161	164	PF00017	0.491
LIG_SH2_STAT5	172	175	PF00017	0.406
LIG_SH2_STAT5	255	258	PF00017	0.377
LIG_SH2_STAT5	504	507	PF00017	0.337
LIG_SH2_STAT5	527	530	PF00017	0.563
LIG_SH2_STAT5	59	62	PF00017	0.478
LIG_SH3_2	300	305	PF14604	0.493
LIG_SH3_3	140	146	PF00018	0.528
LIG_SH3_3	294	300	PF00018	0.351
LIG_SH3_3	303	309	PF00018	0.480
LIG_SH3_3	431	437	PF00018	0.285
LIG_SH3_3	616	622	PF00018	0.792
LIG_SH3_3	694	700	PF00018	0.369
LIG_SUMO_SIM_anti_2	363	368	PF11976	0.281
LIG_SUMO_SIM_anti_2	672	678	PF11976	0.385
LIG_SUMO_SIM_par_1	40	47	PF11976	0.668
LIG_SUMO_SIM_par_1	420	425	PF11976	0.281
LIG_TRAF2_1	229	232	PF00917	0.724
LIG_TRAF2_1	240	243	PF00917	0.655
LIG_TRAF2_1	401	404	PF00917	0.302
LIG_TRAF2_1	94	97	PF00917	0.718
LIG_UBA3_1	415	420	PF00899	0.411
LIG_UBA3_1	534	538	PF00899	0.525
LIG_WRC_WIRS_1	395	400	PF05994	0.393
MOD_CDK_SPK_2	615	620	PF00069	0.513
MOD_CDK_SPK_2	684	689	PF00069	0.559
MOD_CDK_SPxxK_3	29	36	PF00069	0.519
MOD_CK1_1	147	153	PF00069	0.601
MOD_CK1_1	253	259	PF00069	0.362
MOD_CK1_1	260	266	PF00069	0.336
MOD_CK1_1	298	304	PF00069	0.483
MOD_CK1_1	37	43	PF00069	0.626
MOD_CK1_1	546	552	PF00069	0.747
MOD_CK1_1	566	572	PF00069	0.601
MOD_CK1_1	626	632	PF00069	0.616
MOD_CK1_1	66	72	PF00069	0.463
MOD_CK1_1	667	673	PF00069	0.457
MOD_CK1_1	683	689	PF00069	0.531
MOD_CK2_1	100	106	PF00069	0.644
MOD_CK2_1	233	239	PF00069	0.581
MOD_CK2_1	374	380	PF00069	0.318
MOD_CK2_1	387	393	PF00069	0.214
MOD_CK2_1	398	404	PF00069	0.281
MOD_CK2_1	527	533	PF00069	0.540
MOD_CK2_1	578	584	PF00069	0.713
MOD_CK2_1	629	635	PF00069	0.546
MOD_CK2_1	91	97	PF00069	0.589
MOD_GlcNHglycan	128	131	PF01048	0.598
MOD_GlcNHglycan	145	149	PF01048	0.575
MOD_GlcNHglycan	205	208	PF01048	0.708
MOD_GlcNHglycan	223	226	PF01048	0.728
MOD_GlcNHglycan	280	283	PF01048	0.493
MOD_GlcNHglycan	311	314	PF01048	0.631
MOD_GlcNHglycan	318	321	PF01048	0.589
MOD_GlcNHglycan	326	330	PF01048	0.438
MOD_GlcNHglycan	337	340	PF01048	0.189
MOD_GlcNHglycan	345	349	PF01048	0.266
MOD_GlcNHglycan	36	39	PF01048	0.579
MOD_GlcNHglycan	40	43	PF01048	0.581
MOD_GlcNHglycan	568	571	PF01048	0.685
MOD_GlcNHglycan	64	68	PF01048	0.574
MOD_GlcNHglycan	666	669	PF01048	0.510
MOD_GlcNHglycan	671	674	PF01048	0.409
MOD_GSK3_1	210	217	PF00069	0.708
MOD_GSK3_1	253	260	PF00069	0.472
MOD_GSK3_1	309	316	PF00069	0.543
MOD_GSK3_1	34	41	PF00069	0.655
MOD_GSK3_1	383	390	PF00069	0.284
MOD_GSK3_1	394	401	PF00069	0.252
MOD_GSK3_1	483	490	PF00069	0.285
MOD_GSK3_1	506	513	PF00069	0.200
MOD_GSK3_1	542	549	PF00069	0.650
MOD_GSK3_1	561	568	PF00069	0.603
MOD_GSK3_1	624	631	PF00069	0.660
MOD_GSK3_1	680	687	PF00069	0.546
MOD_GSK3_1	695	702	PF00069	0.287
MOD_GSK3_1	96	103	PF00069	0.707
MOD_N-GLC_1	546	551	PF02516	0.774
MOD_NEK2_1	209	214	PF00069	0.788
MOD_NEK2_1	220	225	PF00069	0.652
MOD_NEK2_1	278	283	PF00069	0.439
MOD_NEK2_1	311	316	PF00069	0.542
MOD_NEK2_1	349	354	PF00069	0.473
MOD_NEK2_1	387	392	PF00069	0.299
MOD_NEK2_1	402	407	PF00069	0.259
MOD_NEK2_1	422	427	PF00069	0.399
MOD_NEK2_1	450	455	PF00069	0.411
MOD_NEK2_1	54	59	PF00069	0.492
MOD_NEK2_2	487	492	PF00069	0.411
MOD_PIKK_1	138	144	PF00454	0.335
MOD_PIKK_1	150	156	PF00454	0.549
MOD_PIKK_1	352	358	PF00454	0.412
MOD_PIKK_1	428	434	PF00454	0.314
MOD_PIKK_1	527	533	PF00454	0.610
MOD_PIKK_1	91	97	PF00454	0.662
MOD_PKA_2	100	106	PF00069	0.588
MOD_PKA_2	183	189	PF00069	0.404
MOD_PKA_2	203	209	PF00069	0.584
MOD_PKA_2	214	220	PF00069	0.635
MOD_PKA_2	250	256	PF00069	0.348
MOD_PKA_2	298	304	PF00069	0.481
MOD_PKA_2	456	462	PF00069	0.300
MOD_PKA_2	48	54	PF00069	0.514
MOD_Plk_1	402	408	PF00069	0.328
MOD_Plk_1	428	434	PF00069	0.314
MOD_Plk_1	695	701	PF00069	0.363
MOD_Plk_4	100	106	PF00069	0.638
MOD_Plk_4	250	256	PF00069	0.348
MOD_Plk_4	257	263	PF00069	0.369
MOD_Plk_4	394	400	PF00069	0.295
MOD_Plk_4	436	442	PF00069	0.290
MOD_ProDKin_1	230	236	PF00069	0.524
MOD_ProDKin_1	29	35	PF00069	0.599
MOD_ProDKin_1	615	621	PF00069	0.733
MOD_ProDKin_1	624	630	PF00069	0.751
MOD_ProDKin_1	684	690	PF00069	0.571
MOD_SUMO_for_1	601	604	PF00179	0.607
MOD_SUMO_for_1	688	691	PF00179	0.514
MOD_SUMO_rev_2	530	540	PF00179	0.605
TRG_DiLeu_BaLyEn_6	26	31	PF01217	0.591
TRG_DiLeu_BaLyEn_6	306	311	PF01217	0.568
TRG_DiLeu_BaLyEn_6	411	416	PF01217	0.411
TRG_ENDOCYTIC_2	504	507	PF00928	0.266
TRG_ENDOCYTIC_2	59	62	PF00928	0.592
TRG_ENDOCYTIC_2	78	81	PF00928	0.201
TRG_ER_diArg_1	342	344	PF00400	0.300
TRG_ER_diArg_1	491	493	PF00400	0.281
TRG_ER_diArg_1	70	72	PF00400	0.412
TRG_ER_diArg_1	706	708	PF00400	0.470
TRG_NES_CRM1_1	592	605	PF08389	0.464
TRG_NLS_MonoExtC_3	598	604	PF00514	0.580
TRG_NLS_MonoExtN_4	597	603	PF00514	0.661

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IAT5	Leptomonas seymouri	65%	100%
A0A0S4JBE4	Bodo saltans	31%	100%
A0A1X0NW10	Trypanosomatidae	42%	100%
A0A3Q8IL35	Leishmania donovani	90%	100%
A0A3R7NGI8	Trypanosoma rangeli	42%	100%
A4HKF9	Leishmania braziliensis	77%	100%
A4I7Y7	Leishmania infantum	91%	100%
D0AA49	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
Q4Q5A8	Leishmania major	90%	100%
V5DFV4	Trypanosoma cruzi	41%	100%