Putative RNA guanylyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA capping enzyme, cytoplasmic

Species:

Leishmania mexicana

UniProt:

E9B2R1_LEIMU

TriTrypDb:

LmxM.31.1360

Length:

684

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B2R1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B2R1

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	9
GO:0006370	7-methylguanosine mRNA capping	8	9
GO:0006396	RNA processing	6	9
GO:0006397	mRNA processing	7	9
GO:0006725	cellular aromatic compound metabolic process	3	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008152	metabolic process	1	9
GO:0009452	7-methylguanosine RNA capping	8	9
GO:0009987	cellular process	1	9
GO:0016070	RNA metabolic process	5	9
GO:0016071	mRNA metabolic process	6	9
GO:0034641	cellular nitrogen compound metabolic process	3	9
GO:0036260	RNA capping	7	9
GO:0043170	macromolecule metabolic process	3	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0046483	heterocycle metabolic process	3	9
GO:0071704	organic substance metabolic process	2	9
GO:0090304	nucleic acid metabolic process	4	9
GO:1901360	organic cyclic compound metabolic process	3	9

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	9
GO:0003824	catalytic activity	1	9
GO:0004484	mRNA guanylyltransferase activity	5	9
GO:0005488	binding	1	9
GO:0005524	ATP binding	5	9
GO:0008192	RNA guanylyltransferase activity	4	9
GO:0016740	transferase activity	2	9
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	9
GO:0016779	nucleotidyltransferase activity	4	9
GO:0017076	purine nucleotide binding	4	9
GO:0030554	adenyl nucleotide binding	5	9
GO:0032553	ribonucleotide binding	3	9
GO:0032555	purine ribonucleotide binding	4	9
GO:0032559	adenyl ribonucleotide binding	5	9
GO:0035639	purine ribonucleoside triphosphate binding	4	9
GO:0036094	small molecule binding	2	9
GO:0043167	ion binding	2	9
GO:0043168	anion binding	3	9
GO:0070568	guanylyltransferase activity	5	9
GO:0097159	organic cyclic compound binding	2	9
GO:0097367	carbohydrate derivative binding	2	9
GO:0140098	catalytic activity, acting on RNA	3	9
GO:0140640	catalytic activity, acting on a nucleic acid	2	9
GO:1901265	nucleoside phosphate binding	3	9
GO:1901363	heterocyclic compound binding	2	9
GO:0005525	GTP binding	5	4
GO:0019001	guanyl nucleotide binding	5	4
GO:0019002	GMP binding	4	1
GO:0032561	guanyl ribonucleotide binding	5	4
GO:0003909	DNA ligase activity	4	1
GO:0003910	DNA ligase (ATP) activity	5	1
GO:0016874	ligase activity	2	1
GO:0016886	ligase activity, forming phosphoric ester bonds	3	1
GO:0140097	catalytic activity, acting on DNA	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	296	300	PF00656	0.499
CLV_NRD_NRD_1	123	125	PF00675	0.684
CLV_NRD_NRD_1	240	242	PF00675	0.523
CLV_NRD_NRD_1	288	290	PF00675	0.398
CLV_PCSK_KEX2_1	240	242	PF00082	0.404
CLV_PCSK_KEX2_1	288	290	PF00082	0.398
CLV_PCSK_KEX2_1	304	306	PF00082	0.298
CLV_PCSK_KEX2_1	374	376	PF00082	0.429
CLV_PCSK_KEX2_1	475	477	PF00082	0.352
CLV_PCSK_PC1ET2_1	304	306	PF00082	0.425
CLV_PCSK_PC1ET2_1	374	376	PF00082	0.490
CLV_PCSK_PC1ET2_1	475	477	PF00082	0.426
CLV_PCSK_PC7_1	370	376	PF00082	0.399
CLV_PCSK_SKI1_1	472	476	PF00082	0.431
CLV_PCSK_SKI1_1	548	552	PF00082	0.311
CLV_PCSK_SKI1_1	660	664	PF00082	0.458
CLV_Separin_Metazoa	484	488	PF03568	0.303
DEG_APCC_DBOX_1	155	163	PF00400	0.457
DEG_SCF_FBW7_1	60	65	PF00400	0.530
DEG_SPOP_SBC_1	66	70	PF00917	0.579
DOC_CDC14_PxL_1	514	522	PF14671	0.303
DOC_CKS1_1	313	318	PF01111	0.405
DOC_CYCLIN_RxL_1	548	562	PF00134	0.353
DOC_CYCLIN_RxL_1	669	677	PF00134	0.401
DOC_CYCLIN_yCln2_LP_2	223	229	PF00134	0.292
DOC_CYCLIN_yCln2_LP_2	401	407	PF00134	0.303
DOC_MAPK_gen_1	191	200	PF00069	0.399
DOC_MAPK_gen_1	269	279	PF00069	0.491
DOC_MAPK_gen_1	304	311	PF00069	0.420
DOC_MAPK_gen_1	374	381	PF00069	0.303
DOC_MAPK_gen_1	644	652	PF00069	0.295
DOC_MAPK_MEF2A_6	374	383	PF00069	0.303
DOC_MAPK_MEF2A_6	609	618	PF00069	0.384
DOC_MAPK_RevD_3	274	289	PF00069	0.392
DOC_PP1_RVXF_1	670	677	PF00149	0.403
DOC_PP2B_LxvP_1	223	226	PF13499	0.293
DOC_PP4_FxxP_1	676	679	PF00568	0.420
DOC_PP4_MxPP_1	35	38	PF00568	0.537
DOC_USP7_MATH_1	10	14	PF00917	0.599
DOC_USP7_MATH_1	107	111	PF00917	0.454
DOC_USP7_MATH_1	128	132	PF00917	0.589
DOC_USP7_MATH_1	303	307	PF00917	0.577
DOC_USP7_MATH_1	354	358	PF00917	0.430
DOC_USP7_MATH_1	445	449	PF00917	0.255
DOC_USP7_MATH_1	451	455	PF00917	0.255
DOC_USP7_MATH_1	54	58	PF00917	0.633
DOC_USP7_MATH_1	62	66	PF00917	0.623
DOC_USP7_MATH_1	679	683	PF00917	0.459
DOC_USP7_MATH_1	91	95	PF00917	0.735
DOC_USP7_UBL2_3	548	552	PF12436	0.271
DOC_WW_Pin1_4	103	108	PF00397	0.666
DOC_WW_Pin1_4	23	28	PF00397	0.524
DOC_WW_Pin1_4	312	317	PF00397	0.396
DOC_WW_Pin1_4	474	479	PF00397	0.303
DOC_WW_Pin1_4	58	63	PF00397	0.755
LIG_14-3-3_CanoR_1	375	380	PF00244	0.263
LIG_14-3-3_CanoR_1	609	616	PF00244	0.387
LIG_Actin_WH2_2	224	242	PF00022	0.384
LIG_Actin_WH2_2	594	611	PF00022	0.402
LIG_APCC_ABBA_1	595	600	PF00400	0.381
LIG_APCC_Cbox_2	201	207	PF00515	0.398
LIG_BIR_II_1	1	5	PF00653	0.693
LIG_BIR_III_3	1	5	PF00653	0.609
LIG_BRCT_BRCA1_1	418	422	PF00533	0.245
LIG_EH1_1	528	536	PF00400	0.303
LIG_eIF4E_1	320	326	PF01652	0.379
LIG_eIF4E_1	411	417	PF01652	0.271
LIG_FHA_1	334	340	PF00498	0.437
LIG_FHA_1	380	386	PF00498	0.281
LIG_FHA_1	434	440	PF00498	0.204
LIG_FHA_1	631	637	PF00498	0.516
LIG_FHA_1	82	88	PF00498	0.476
LIG_FHA_2	146	152	PF00498	0.426
LIG_FHA_2	448	454	PF00498	0.221
LIG_FHA_2	574	580	PF00498	0.391
LIG_FHA_2	581	587	PF00498	0.563
LIG_FHA_2	661	667	PF00498	0.447
LIG_Integrin_RGD_1	245	247	PF01839	0.513
LIG_LIR_Apic_2	115	120	PF02991	0.623
LIG_LIR_Apic_2	26	31	PF02991	0.543
LIG_LIR_Gen_1	155	162	PF02991	0.455
LIG_LIR_Gen_1	321	329	PF02991	0.380
LIG_LIR_Gen_1	330	339	PF02991	0.280
LIG_LIR_Gen_1	377	385	PF02991	0.387
LIG_LIR_Gen_1	388	398	PF02991	0.387
LIG_LIR_Gen_1	412	421	PF02991	0.334
LIG_LIR_Gen_1	423	431	PF02991	0.321
LIG_LIR_Gen_1	465	474	PF02991	0.253
LIG_LIR_Gen_1	481	490	PF02991	0.253
LIG_LIR_Gen_1	576	584	PF02991	0.386
LIG_LIR_Gen_1	663	668	PF02991	0.444
LIG_LIR_Nem_3	155	160	PF02991	0.437
LIG_LIR_Nem_3	192	198	PF02991	0.415
LIG_LIR_Nem_3	253	258	PF02991	0.542
LIG_LIR_Nem_3	268	274	PF02991	0.267
LIG_LIR_Nem_3	321	326	PF02991	0.389
LIG_LIR_Nem_3	327	332	PF02991	0.339
LIG_LIR_Nem_3	377	383	PF02991	0.339
LIG_LIR_Nem_3	388	393	PF02991	0.356
LIG_LIR_Nem_3	396	401	PF02991	0.342
LIG_LIR_Nem_3	412	416	PF02991	0.243
LIG_LIR_Nem_3	423	429	PF02991	0.255
LIG_LIR_Nem_3	465	469	PF02991	0.253
LIG_LIR_Nem_3	477	483	PF02991	0.253
LIG_LIR_Nem_3	488	492	PF02991	0.233
LIG_LIR_Nem_3	576	581	PF02991	0.468
LIG_LIR_Nem_3	663	667	PF02991	0.451
LIG_MYND_1	518	522	PF01753	0.303
LIG_PCNA_APIM_2	546	552	PF02747	0.303
LIG_PCNA_TLS_4	543	550	PF02747	0.303
LIG_Pex14_2	403	407	PF04695	0.253
LIG_Pex14_2	413	417	PF04695	0.253
LIG_Pex14_2	422	426	PF04695	0.253
LIG_Pex14_2	549	553	PF04695	0.271
LIG_REV1ctd_RIR_1	477	486	PF16727	0.387
LIG_SH2_CRK	157	161	PF00017	0.434
LIG_SH2_CRK	195	199	PF00017	0.329
LIG_SH2_CRK	271	275	PF00017	0.338
LIG_SH2_CRK	503	507	PF00017	0.255
LIG_SH2_PTP2	380	383	PF00017	0.303
LIG_SH2_PTP2	511	514	PF00017	0.271
LIG_SH2_SRC	361	364	PF00017	0.372
LIG_SH2_SRC	390	393	PF00017	0.175
LIG_SH2_SRC	511	514	PF00017	0.253
LIG_SH2_STAP1	16	20	PF00017	0.606
LIG_SH2_STAP1	411	415	PF00017	0.428
LIG_SH2_STAP1	503	507	PF00017	0.255
LIG_SH2_STAT3	212	215	PF00017	0.414
LIG_SH2_STAT3	257	260	PF00017	0.433
LIG_SH2_STAT5	213	216	PF00017	0.281
LIG_SH2_STAT5	257	260	PF00017	0.584
LIG_SH2_STAT5	361	364	PF00017	0.345
LIG_SH2_STAT5	380	383	PF00017	0.341
LIG_SH2_STAT5	390	393	PF00017	0.230
LIG_SH2_STAT5	466	469	PF00017	0.269
LIG_SH2_STAT5	511	514	PF00017	0.253
LIG_SH3_1	310	316	PF00018	0.356
LIG_SH3_3	310	316	PF00018	0.478
LIG_SH3_3	45	51	PF00018	0.709
LIG_SH3_3	586	592	PF00018	0.490
LIG_SH3_3	639	645	PF00018	0.453
LIG_SUMO_SIM_par_1	610	617	PF11976	0.467
LIG_TRAF2_1	663	666	PF00917	0.369
LIG_TYR_ITIM	662	667	PF00017	0.446
LIG_UBA3_1	297	304	PF00899	0.479
MOD_CK1_1	106	112	PF00069	0.756
MOD_CK1_1	144	150	PF00069	0.460
MOD_CK1_1	347	353	PF00069	0.559
MOD_CK1_1	65	71	PF00069	0.796
MOD_CK1_1	94	100	PF00069	0.687
MOD_CK2_1	145	151	PF00069	0.483
MOD_CK2_1	447	453	PF00069	0.438
MOD_CK2_1	580	586	PF00069	0.571
MOD_CK2_1	648	654	PF00069	0.470
MOD_CK2_1	660	666	PF00069	0.288
MOD_Cter_Amidation	122	125	PF01082	0.539
MOD_GlcNHglycan	100	103	PF01048	0.698
MOD_GlcNHglycan	114	117	PF01048	0.617
MOD_GlcNHglycan	12	15	PF01048	0.599
MOD_GlcNHglycan	143	146	PF01048	0.342
MOD_GlcNHglycan	16	19	PF01048	0.573
MOD_GlcNHglycan	163	166	PF01048	0.144
MOD_GlcNHglycan	346	349	PF01048	0.447
MOD_GlcNHglycan	351	354	PF01048	0.364
MOD_GlcNHglycan	496	499	PF01048	0.395
MOD_GlcNHglycan	610	613	PF01048	0.406
MOD_GlcNHglycan	64	67	PF01048	0.704
MOD_GlcNHglycan	676	679	PF01048	0.373
MOD_GlcNHglycan	69	72	PF01048	0.701
MOD_GlcNHglycan	88	91	PF01048	0.616
MOD_GSK3_1	10	17	PF00069	0.603
MOD_GSK3_1	103	110	PF00069	0.564
MOD_GSK3_1	141	148	PF00069	0.480
MOD_GSK3_1	343	350	PF00069	0.424
MOD_GSK3_1	375	382	PF00069	0.289
MOD_GSK3_1	416	423	PF00069	0.221
MOD_GSK3_1	447	454	PF00069	0.307
MOD_GSK3_1	50	57	PF00069	0.753
MOD_GSK3_1	58	65	PF00069	0.790
MOD_GSK3_1	66	73	PF00069	0.557
MOD_GSK3_1	91	98	PF00069	0.662
MOD_N-GLC_1	344	349	PF02516	0.478
MOD_N-GLC_1	679	684	PF02516	0.523
MOD_N-GLC_2	219	221	PF02516	0.320
MOD_NEK2_1	160	165	PF00069	0.426
MOD_NEK2_1	379	384	PF00069	0.284
MOD_NEK2_1	405	410	PF00069	0.303
MOD_NEK2_1	416	421	PF00069	0.215
MOD_NEK2_1	447	452	PF00069	0.311
MOD_NEK2_1	608	613	PF00069	0.406
MOD_NEK2_1	674	679	PF00069	0.420
MOD_NEK2_1	96	101	PF00069	0.704
MOD_PIKK_1	145	151	PF00454	0.422
MOD_PIKK_1	630	636	PF00454	0.614
MOD_PK_1	293	299	PF00069	0.359
MOD_PKA_1	374	380	PF00069	0.303
MOD_PKA_2	141	147	PF00069	0.517
MOD_PKA_2	374	380	PF00069	0.303
MOD_PKA_2	608	614	PF00069	0.547
MOD_PKA_2	674	680	PF00069	0.428
MOD_Plk_1	191	197	PF00069	0.434
MOD_Plk_2-3	573	579	PF00069	0.556
MOD_Plk_4	293	299	PF00069	0.459
MOD_Plk_4	375	381	PF00069	0.303
MOD_Plk_4	416	422	PF00069	0.271
MOD_Plk_4	537	543	PF00069	0.303
MOD_Plk_4	573	579	PF00069	0.405
MOD_Plk_4	648	654	PF00069	0.391
MOD_ProDKin_1	103	109	PF00069	0.666
MOD_ProDKin_1	23	29	PF00069	0.529
MOD_ProDKin_1	312	318	PF00069	0.404
MOD_ProDKin_1	474	480	PF00069	0.303
MOD_ProDKin_1	58	64	PF00069	0.753
MOD_SUMO_rev_2	164	174	PF00179	0.454
MOD_SUMO_rev_2	366	376	PF00179	0.398
TRG_DiLeu_BaEn_1	155	160	PF01217	0.457
TRG_DiLeu_BaEn_1	481	486	PF01217	0.303
TRG_DiLeu_BaEn_1	488	493	PF01217	0.247
TRG_DiLeu_BaEn_1	516	521	PF01217	0.255
TRG_DiLeu_BaLyEn_6	130	135	PF01217	0.410
TRG_DiLeu_BaLyEn_6	456	461	PF01217	0.303
TRG_ENDOCYTIC_2	157	160	PF00928	0.434
TRG_ENDOCYTIC_2	195	198	PF00928	0.334
TRG_ENDOCYTIC_2	271	274	PF00928	0.364
TRG_ENDOCYTIC_2	320	323	PF00928	0.410
TRG_ENDOCYTIC_2	329	332	PF00928	0.280
TRG_ENDOCYTIC_2	380	383	PF00928	0.403
TRG_ENDOCYTIC_2	390	393	PF00928	0.238
TRG_ENDOCYTIC_2	466	469	PF00928	0.330
TRG_ENDOCYTIC_2	483	486	PF00928	0.330
TRG_ENDOCYTIC_2	503	506	PF00928	0.144
TRG_ENDOCYTIC_2	511	514	PF00928	0.245
TRG_ENDOCYTIC_2	664	667	PF00928	0.406
TRG_ER_diArg_1	239	241	PF00400	0.539
TRG_ER_diArg_1	288	290	PF00400	0.398
TRG_ER_diArg_1	639	642	PF00400	0.447
TRG_NES_CRM1_1	222	237	PF08389	0.369
TRG_NLS_Bipartite_1	288	308	PF00514	0.389
TRG_NLS_MonoExtN_4	303	308	PF00514	0.426

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4IM27	Bodo saltans	34%	95%
A0A1X0NUI5	Trypanosomatidae	39%	100%
A0A3S7X5E2	Leishmania donovani	91%	99%
A0A422P1D1	Trypanosoma rangeli	41%	100%
A4HKC4	Leishmania braziliensis	83%	100%
D0AA12	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
Q4Q5E6	Leishmania major	92%	100%
V5DHN7	Trypanosoma cruzi	42%	100%