LeishMANIAdb
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Phenylalanine--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:
Phenylalanine--tRNA ligase
Gene product:
phenylalanyl-tRNA synthetase alpha chain, putative
Species:
Leishmania mexicana
UniProt:
E9B2L1_LEIMU
TriTrypDb:
LmxM.31.0870
Length:
499

Localization

Secreted promastigote
Source Evidence on protein Close homologs
Cuervo et al. no yes: 0
Hassani et al. no yes: 0
Forrest at al. (metacyclic) no yes: 0
Forrest at al. (procyclic) no yes: 3
Silverman et al. no yes: 0
Pissara et al. no yes: 0
Secreted amastigote
Source Evidence on protein Close homologs
Pires et al. no yes: 0
Exosome
Source Evidence on protein Close homologs
Silverman et al. no yes: 0
Glycosome
Source Evidence on protein Close homologs
Jamdhade et al. no yes: 8
Predictions
Source Evidence on protein Close homologs
DeepLoc
SignalP6 no yes: 0, no: 11
NetGPI no yes: 0, no: 11
Cellular components
Term Name Level Count
GO:0005737 cytoplasm 2 12
GO:0110165 cellular anatomical entity 1 12
GO:0009328 phenylalanine-tRNA ligase complex 3 1
GO:0032991 protein-containing complex 1 1
GO:1902494 catalytic complex 2 1

Expansion

Sequence features

E9B2L1
Sequence
MSA
Disorder
Secondary
Topology
Domains
SignalP
GPI
Phosphorylations
ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B2L1

Function

Biological processes
Term Name Level Count
GO:0006082 organic acid metabolic process 3 12
GO:0006139 nucleobase-containing compound metabolic process 3 12
GO:0006399 tRNA metabolic process 7 12
GO:0006418 tRNA aminoacylation for protein translation 6 12
GO:0006432 phenylalanyl-tRNA aminoacylation 7 12
GO:0006520 amino acid metabolic process 3 12
GO:0006725 cellular aromatic compound metabolic process 3 12
GO:0006807 nitrogen compound metabolic process 2 12
GO:0008152 metabolic process 1 12
GO:0009987 cellular process 1 12
GO:0016070 RNA metabolic process 5 12
GO:0019752 carboxylic acid metabolic process 5 12
GO:0034641 cellular nitrogen compound metabolic process 3 12
GO:0034660 ncRNA metabolic process 6 12
GO:0043038 amino acid activation 4 12
GO:0043039 tRNA aminoacylation 5 12
GO:0043170 macromolecule metabolic process 3 12
GO:0043436 oxoacid metabolic process 4 12
GO:0044237 cellular metabolic process 2 12
GO:0044238 primary metabolic process 2 12
GO:0044281 small molecule metabolic process 2 12
GO:0046483 heterocycle metabolic process 3 12
GO:0071704 organic substance metabolic process 2 12
GO:0090304 nucleic acid metabolic process 4 12
GO:1901360 organic cyclic compound metabolic process 3 12
GO:1901564 organonitrogen compound metabolic process 3 12
Molecular functions
Term Name Level Count
GO:0000049 tRNA binding 5 12
GO:0000166 nucleotide binding 3 12
GO:0003676 nucleic acid binding 3 12
GO:0003723 RNA binding 4 12
GO:0003824 catalytic activity 1 12
GO:0004812 aminoacyl-tRNA ligase activity 4 12
GO:0004826 phenylalanine-tRNA ligase activity 5 12
GO:0005488 binding 1 12
GO:0005524 ATP binding 5 12
GO:0016874 ligase activity 2 12
GO:0016875 ligase activity, forming carbon-oxygen bonds 3 12
GO:0017076 purine nucleotide binding 4 12
GO:0030554 adenyl nucleotide binding 5 12
GO:0032553 ribonucleotide binding 3 12
GO:0032555 purine ribonucleotide binding 4 12
GO:0032559 adenyl ribonucleotide binding 5 12
GO:0035639 purine ribonucleoside triphosphate binding 4 12
GO:0036094 small molecule binding 2 12
GO:0043167 ion binding 2 12
GO:0043168 anion binding 3 12
GO:0097159 organic cyclic compound binding 2 12
GO:0097367 carbohydrate derivative binding 2 12
GO:0140098 catalytic activity, acting on RNA 3 12
GO:0140101 catalytic activity, acting on a tRNA 4 12
GO:0140640 catalytic activity, acting on a nucleic acid 2 12
GO:1901265 nucleoside phosphate binding 3 12
GO:1901363 heterocyclic compound binding 2 12

Putative motif mimicry

Leishmania From To Domain/Motif Score
CLV_NRD_NRD_1 162 164 PF00675 0.389
CLV_NRD_NRD_1 400 402 PF00675 0.251
CLV_PCSK_KEX2_1 162 164 PF00082 0.400
CLV_PCSK_KEX2_1 400 402 PF00082 0.251
CLV_PCSK_KEX2_1 492 494 PF00082 0.459
CLV_PCSK_PC1ET2_1 162 164 PF00082 0.400
CLV_PCSK_PC1ET2_1 492 494 PF00082 0.459
CLV_PCSK_SKI1_1 112 116 PF00082 0.320
CLV_PCSK_SKI1_1 122 126 PF00082 0.418
CLV_PCSK_SKI1_1 171 175 PF00082 0.389
CLV_PCSK_SKI1_1 186 190 PF00082 0.353
CLV_PCSK_SKI1_1 196 200 PF00082 0.366
CLV_PCSK_SKI1_1 207 211 PF00082 0.388
CLV_PCSK_SKI1_1 237 241 PF00082 0.234
CLV_PCSK_SKI1_1 317 321 PF00082 0.248
CLV_PCSK_SKI1_1 410 414 PF00082 0.209
CLV_PCSK_SKI1_1 489 493 PF00082 0.225
CLV_PCSK_SKI1_1 56 60 PF00082 0.451
DEG_APCC_DBOX_1 236 244 PF00400 0.512
DEG_Nend_UBRbox_2 1 3 PF02207 0.530
DOC_CKS1_1 306 311 PF01111 0.512
DOC_CYCLIN_yCln2_LP_2 446 452 PF00134 0.512
DOC_MAPK_gen_1 115 125 PF00069 0.488
DOC_MAPK_gen_1 95 105 PF00069 0.411
DOC_MAPK_MEF2A_6 237 245 PF00069 0.419
DOC_PP1_RVXF_1 344 350 PF00149 0.512
DOC_PP2B_LxvP_1 264 267 PF13499 0.451
DOC_PP4_FxxP_1 306 309 PF00568 0.512
DOC_USP7_MATH_1 13 17 PF00917 0.489
DOC_USP7_MATH_1 254 258 PF00917 0.464
DOC_USP7_MATH_1 284 288 PF00917 0.527
DOC_USP7_MATH_1 39 43 PF00917 0.409
DOC_USP7_UBL2_3 118 122 PF12436 0.433
DOC_USP7_UBL2_3 333 337 PF12436 0.457
DOC_WW_Pin1_4 305 310 PF00397 0.512
DOC_WW_Pin1_4 70 75 PF00397 0.328
LIG_14-3-3_CanoR_1 19 29 PF00244 0.533
LIG_14-3-3_CanoR_1 321 330 PF00244 0.409
LIG_14-3-3_CanoR_1 401 411 PF00244 0.515
LIG_14-3-3_CanoR_1 86 92 PF00244 0.399
LIG_Actin_WH2_2 46 62 PF00022 0.430
LIG_APCC_ABBA_1 413 418 PF00400 0.409
LIG_BRCT_BRCA1_1 110 114 PF00533 0.442
LIG_BRCT_BRCA1_1 326 330 PF00533 0.512
LIG_BRCT_BRCA1_1 438 442 PF00533 0.409
LIG_deltaCOP1_diTrp_1 201 209 PF00928 0.366
LIG_eIF4E_1 76 82 PF01652 0.470
LIG_FHA_1 152 158 PF00498 0.483
LIG_FHA_1 276 282 PF00498 0.422
LIG_FHA_1 284 290 PF00498 0.434
LIG_FHA_1 98 104 PF00498 0.420
LIG_FHA_2 114 120 PF00498 0.442
LIG_GBD_Chelix_1 105 113 PF00786 0.462
LIG_IBAR_NPY_1 414 416 PF08397 0.409
LIG_LIR_Apic_2 303 309 PF02991 0.512
LIG_LIR_Gen_1 242 249 PF02991 0.413
LIG_LIR_Gen_1 250 261 PF02991 0.437
LIG_LIR_Gen_1 373 382 PF02991 0.409
LIG_LIR_Gen_1 394 402 PF02991 0.419
LIG_LIR_Nem_3 235 239 PF02991 0.419
LIG_LIR_Nem_3 250 256 PF02991 0.427
LIG_LIR_Nem_3 257 261 PF02991 0.515
LIG_LIR_Nem_3 373 377 PF02991 0.409
LIG_LIR_Nem_3 394 398 PF02991 0.419
LIG_LRP6_Inhibitor_1 104 110 PF00058 0.504
LIG_Pex14_2 306 310 PF04695 0.419
LIG_Pex14_2 395 399 PF04695 0.422
LIG_Pex14_2 409 413 PF04695 0.480
LIG_REV1ctd_RIR_1 277 283 PF16727 0.409
LIG_REV1ctd_RIR_1 396 405 PF16727 0.409
LIG_SH2_NCK_1 416 420 PF00017 0.409
LIG_SH2_STAT5 176 179 PF00017 0.484
LIG_SH2_STAT5 416 419 PF00017 0.409
LIG_SH2_STAT5 48 51 PF00017 0.329
LIG_SH2_STAT5 76 79 PF00017 0.447
LIG_SUMO_SIM_anti_2 453 460 PF11976 0.419
LIG_WRC_WIRS_1 392 397 PF05994 0.427
MOD_CDK_SPxK_1 305 311 PF00069 0.512
MOD_CK1_1 305 311 PF00069 0.431
MOD_CK2_1 113 119 PF00069 0.426
MOD_CK2_1 12 18 PF00069 0.505
MOD_CK2_1 279 285 PF00069 0.523
MOD_CK2_1 48 54 PF00069 0.463
MOD_GlcNHglycan 15 18 PF01048 0.299
MOD_GlcNHglycan 214 217 PF01048 0.312
MOD_GlcNHglycan 327 330 PF01048 0.224
MOD_GlcNHglycan 438 441 PF01048 0.209
MOD_GlcNHglycan 70 73 PF01048 0.383
MOD_GSK3_1 275 282 PF00069 0.427
MOD_GSK3_1 321 328 PF00069 0.457
MOD_GSK3_1 44 51 PF00069 0.499
MOD_N-GLC_1 212 217 PF02516 0.267
MOD_NEK2_1 113 118 PF00069 0.440
MOD_NEK2_1 174 179 PF00069 0.387
MOD_NEK2_1 275 280 PF00069 0.417
MOD_NEK2_1 366 371 PF00069 0.409
MOD_PIKK_1 247 253 PF00454 0.475
MOD_PIKK_1 402 408 PF00454 0.512
MOD_PIKK_1 85 91 PF00454 0.450
MOD_PK_1 353 359 PF00069 0.409
MOD_PKA_2 138 144 PF00069 0.422
MOD_PKA_2 466 472 PF00069 0.409
MOD_PKA_2 85 91 PF00069 0.379
MOD_Plk_1 254 260 PF00069 0.414
MOD_Plk_1 302 308 PF00069 0.532
MOD_Plk_1 366 372 PF00069 0.410
MOD_Plk_1 384 390 PF00069 0.471
MOD_Plk_2-3 153 159 PF00069 0.511
MOD_Plk_4 254 260 PF00069 0.429
MOD_Plk_4 275 281 PF00069 0.420
MOD_Plk_4 394 400 PF00069 0.512
MOD_Plk_4 44 50 PF00069 0.435
MOD_Plk_4 454 460 PF00069 0.413
MOD_Plk_4 466 472 PF00069 0.403
MOD_ProDKin_1 305 311 PF00069 0.512
MOD_ProDKin_1 70 76 PF00069 0.329
MOD_SUMO_rev_2 119 124 PF00179 0.383
MOD_SUMO_rev_2 150 157 PF00179 0.375
MOD_SUMO_rev_2 340 347 PF00179 0.376
TRG_DiLeu_BaLyEn_6 225 230 PF01217 0.418
TRG_ENDOCYTIC_2 354 357 PF00928 0.409
TRG_ER_diArg_1 399 401 PF00400 0.451
TRG_NLS_MonoCore_2 160 165 PF00514 0.361
TRG_NLS_MonoExtN_4 161 166 PF00514 0.358
TRG_Pf-PMV_PEXEL_1 19 24 PF00026 0.225
TRG_Pf-PMV_PEXEL_1 273 277 PF00026 0.312

Homologs

Protein Taxonomy Sequence identity Coverage
A0A0N1IM48 Leptomonas seymouri 82% 100%
A0A0S4KIH9 Bodo saltans 61% 100%
A0A1X0NV30 Trypanosomatidae 62% 100%
A0A3Q8IG22 Leishmania donovani 95% 100%
A0A422N8Y7 Trypanosoma rangeli 62% 100%
A0LFC6 Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) 30% 100%
A1TR35 Acidovorax citrulli (strain AAC00-1) 28% 100%
A1W8I2 Acidovorax sp. (strain JS42) 28% 100%
A1WMG9 Verminephrobacter eiseniae (strain EF01-2) 28% 100%
A2SH03 Methylibium petroleiphilum (strain ATCC BAA-1232 / LMG 22953 / PM1) 26% 100%
A2ST81 Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) 32% 100%
A3MJU2 Burkholderia mallei (strain NCTC 10247) 27% 100%
A3MUA4 Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1) 28% 100%
A3NUI8 Burkholderia pseudomallei (strain 1106a) 27% 100%
A4FW27 Methanococcus maripaludis (strain C5 / ATCC BAA-1333) 32% 100%
A4HK68 Leishmania braziliensis 89% 100%
A4I7Q3 Leishmania infantum 96% 100%
A4JDU8 Burkholderia vietnamiensis (strain G4 / LMG 22486) 28% 100%
A4WHY5 Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6) 30% 100%
A4YIL1 Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) 30% 100%
A5K9S0 Plasmodium vivax (strain Salvador I) 38% 88%
A5W5D7 Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1) 26% 100%
A6UQD9 Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB) 33% 100%
A6VH81 Methanococcus maripaludis (strain C7 / ATCC BAA-1331) 32% 100%
A8FG17 Bacillus pumilus (strain SAFR-032) 26% 100%
A8ZZ70 Desulfococcus oleovorans (strain DSM 6200 / JCM 39069 / Hxd3) 27% 100%
A9C3D0 Delftia acidovorans (strain DSM 14801 / SPH-1) 27% 100%
B0KKR4 Pseudomonas putida (strain GB-1) 26% 100%
B0R806 Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) 36% 99%
B0TEV9 Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) 27% 100%
B3PL12 Cellvibrio japonicus (strain Ueda107) 28% 100%
B3PYE2 Rhizobium etli (strain CIAT 652) 26% 100%
B4E7I9 Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) 28% 100%
B5XKQ6 Streptococcus pyogenes serotype M49 (strain NZ131) 26% 100%
B6YTJ4 Thermococcus onnurineus (strain NA1) 29% 100%
B7XI98 Enterocytozoon bieneusi (strain H348) 37% 100%
B8FFU3 Desulfatibacillum aliphaticivorans 28% 100%
B8G0L8 Desulfitobacterium hafniense (strain DSM 10664 / DCB-2) 29% 100%
B9JYN0 Agrobacterium vitis (strain S4 / ATCC BAA-846) 26% 100%
B9MHY2 Acidovorax ebreus (strain TPSY) 28% 100%
C4V6K5 Nosema ceranae (strain BRL01) 39% 100%
C5A5Y9 Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) 29% 100%
C6A236 Thermococcus sibiricus (strain DSM 12597 / MM 739) 30% 99%
D0A9V8 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 61% 100%
O26837 Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) 29% 98%
O28324 Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) 33% 100%
O42870 Schizosaccharomyces pombe (strain 972 / ATCC 24843) 43% 100%
O58391 Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) 30% 100%
P0DG50 Streptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315) 26% 100%
P0DG51 Streptococcus pyogenes serotype M3 (strain SSI-1) 26% 100%
P15625 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) 43% 99%
P17921 Bacillus subtilis (strain 168) 27% 100%
P57693 Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) 30% 100%
P95961 Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) 29% 100%
Q0W0X9 Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50) 32% 100%
Q1JPX3 Danio rerio 44% 100%
Q1MMP3 Rhizobium leguminosarum bv. viciae (strain 3841) 25% 100%
Q1MRV5 Lawsonia intracellularis (strain PHE/MN1-00) 27% 100%
Q1QWK3 Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11) 27% 100%
Q21YS9 Albidiferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118) 28% 100%
Q251I2 Desulfitobacterium hafniense (strain Y51) 29% 100%
Q2FLL0 Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1) 32% 100%
Q2SVE2 Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264) 28% 100%
Q39H51 Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383) 29% 100%
Q3JT08 Burkholderia pseudomallei (strain 1710b) 27% 100%
Q46CQ6 Methanosarcina barkeri (strain Fusaro / DSM 804) 32% 100%
Q4J8P9 Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) 27% 100%
Q4Q5J5 Leishmania major 96% 100%
Q505J8 Rattus norvegicus 44% 98%
Q54KS8 Dictyostelium discoideum 45% 100%
Q57911 Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) 33% 100%
Q5NMC2 Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) 27% 100%
Q5RFA2 Pongo abelii 43% 98%
Q5XCX4 Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) 26% 100%
Q5ZJQ2 Gallus gallus 43% 100%
Q62KI6 Burkholderia mallei (strain ATCC 23344) 27% 100%
Q63TM6 Burkholderia pseudomallei (strain K96243) 27% 100%
Q6AZG6 Xenopus laevis 45% 100%
Q6LX56 Methanococcus maripaludis (strain S2 / LL) 35% 100%
Q76KA8 Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) 29% 100%
Q7SYV0 Xenopus laevis 45% 100%
Q88K23 Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) 26% 100%
Q891T7 Clostridium tetani (strain Massachusetts / E88) 26% 100%
Q89WI1 Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) 28% 100%
Q8C0C7 Mus musculus 44% 98%
Q8EPH4 Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831) 27% 100%
Q8P1K1 Streptococcus pyogenes serotype M18 (strain MGAS8232) 26% 100%
Q8PWV6 Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) 32% 100%
Q8SRG9 Encephalitozoon cuniculi (strain GB-M1) 42% 100%
Q8TUA2 Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) 33% 100%
Q8TYM5 Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) 34% 95%
Q8ZX61 Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2) 33% 100%
Q971D7 Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) 29% 100%
Q979U4 Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) 30% 100%
Q9A0I1 Streptococcus pyogenes serotype M1 26% 100%
Q9HMK4 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) 36% 99%
Q9T034 Arabidopsis thaliana 45% 100%
Q9UYX3 Pyrococcus abyssi (strain GE5 / Orsay) 28% 100%
Q9W3J5 Drosophila melanogaster 45% 100%
Q9Y285 Homo sapiens 43% 98%
Q9Y9I6 Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) 30% 100%
V5B123 Trypanosoma cruzi 61% 100%

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LeishMANIAdb - Server version: v0.0.2. - Database version: v0.0.1. - ChangeLog - © 2022-2025 Protein Bioinformatics Research Group, Institute of Enzymology, RCNS